Tau Antibody (pSer202/ pThr205)
StressMarq Biosciences
catalog: SMC-601

domestic rabbit monoclonal (AH36)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 3

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36 (SMC-601). Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody (SMC-601) at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 μm stacks.
quantity: 100 µg
price:
to the supplier

Tau Antibody
StressMarq Biosciences
catalog: SMC-607

mouse monoclonal (1D5)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
citations: 2

quantity: 100 µg
price:
to the supplier

Tau Antibody
StressMarq Biosciences
catalog: SMC-608

mouse monoclonal (3D4)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
citations: 3

quantity: 100 µg
price:
to the supplier

Tau Antibody (pSer396)
StressMarq Biosciences
catalog: SPC-1436

domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, ELISA
citations: 1

Western blot analysis of Mouse Brain cell lysates showing detection of ~47-70 kDa Tau (pSer396) protein using Rabbit Anti-Tau (pSer396) Polyclonal Antibody (SPC-1436). Lane 1: Mouse brain cell lysates and antibody incubated with the immunizing peptide. Lane 2: Mouse brain cell lysates. Load: 10 µg. Primary Antibody: Rabbit Anti-Tau (pSer396) Polyclonal Antibody (SPC-1436) at 1:1000. Predicted/Observed Size: ~47-70 kDa.

Western blot analysis of Mouse Brain cell lysates showing detection of ~47-70 kDa Tau (pSer396) protein using Rabbit Anti-Tau (pSer396) Polyclonal Antibody (SPC-1436). Lane 1: Mouse brain cell lysates and antibody incubated with the immunizing peptide. Lane 2: Mouse brain cell lysates. Load: 10 µg. Primary Antibody: Rabbit Anti-Tau (pSer396) Polyclonal Antibody (SPC-1436) at 1:1000. Predicted/Observed Size: ~47-70 kDa.
quantity: 100 µl
price:
to the supplier

Tau Antibody (pSer422)
StressMarq Biosciences
catalog: SPC-1437

domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry
citations: 1

quantity: 100 µl
price:
to the supplier

Tau Antibody
StressMarq Biosciences
catalog: SPC-801

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Western blot analysis of Human SH-SY5Y cell lysate showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (SPC-801). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human SH-SY5Y cell lysate. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (SPC-801) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.

Western blot analysis of Human SH-SY5Y cell lysate showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (SPC-801). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human SH-SY5Y cell lysate. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (SPC-801) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.
quantity: 100 µg
price:
to the supplier

Tau Antibody
StressMarq Biosciences
catalog: SPC-802

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry

Western blot analysis of Human SH-SY5Y cell lysate showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (SPC-802). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human SH-SY5Y cell lysate. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (SPC-802) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.

Western blot analysis of Human SH-SY5Y cell lysate showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody (SPC-802). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human SH-SY5Y cell lysate. Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody (SPC-802) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa.
quantity: 100 µg
price:
to the supplier

Tau Antibody
StressMarq Biosciences
catalog: SPC-806

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

quantity: 100 µg
price:
to the supplier

Tau Antibody (pThr217)
StressMarq Biosciences
catalog: SMC-615

mouse monoclonal (15B7)
reactivity: human, mouse, rat
application: western blot

Dot Blot analysis of Human, Mouse, Rat Tau Peptide (pThr217) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (SMC-615). Panel A: hTau 2N4R monomer (pSer214) (2 ug). Panel B: Tau peptide (pThr217) (2 ug). Panel C: Tau peptide (non phospho) (2 ug). Load: 2ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-615) at 1:1000 for 1 hour at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:H&L at 1:4000 for 1/2 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Phospho peptide sequence: SRTPSLP-pT-PPTREPK. Non-phospho peptide sequence: SRTPSLPTPPTREPK. This sequence is the same for human, mouse, and rat.

Dot Blot analysis of Human, Mouse, Rat Tau 2N4R P301S (phospho & non-phospho) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (SMC-615). Lane 1: hTau 2N4R P301S Monomer(0.5ug). Lane 2: hTau 2N4R P301S Monomer(1.0ug). Lane 3: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(0.5ug). Lane 4: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(1.0ug). Load: 0.5ug, 1.0ug. Block: 5% BSA in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-615) at 1:4 for Overnight, 4C, with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Dot Blot performed with supernatants.

Dot Blot analysis of Human, Mouse, Rat Tau 2N4R P301S (phospho & non-phospho) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (SMC-615). Lane 1: hTau 2N4R P301S Monomer(0.5ug). Lane 2: hTau 2N4R P301S Monomer(1.0ug). Lane 3: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(0.5ug). Lane 4: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(1.0ug). Load: 0.5ug, 1.0ug. Block: 5% BSA in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-615) at 1:4 for Overnight, 4C, with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Dot Blot performed with supernatants.
quantity: 100 µg
price:
to the supplier