mouse monoclonal (8A7)
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
citations: 1
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS
citations: 1
Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (SMC-114). Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (SMC-114) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation. Courtesy of: Mike Welsh, Umich.
quantity: 100 µg
price: 254 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, dogs
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS
reactivity: human, mouse, dogs
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-106). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-106) at 1:250 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
quantity: 200 µg
price: 250 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~27kDa Hsp27 protein using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263). Lane 1 and Lane 2: Human HeLa, Hsp27 Antibody (Ser15). Lane 3 and Lane 4: Human HeLa, Hsp27 Antibody (pSer15). Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-1263) at 1:1000. Predicted/Observed Size: ~27kDa.
quantity: 100 µl
price: 260 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~27kDa HSP27 protein using Rabbit Anti-HSP27 Polyclonal Antibody (SPC-1264). Lane 1 and Lane 2: Human HeLa untreated or treated with Ca2+, Hsp27 Antibody (Ser82). Lane 3 and Lane 4: Human HeLa untreated or treated with Ca2+, Hsp27 Antibody (pSer82). Primary Antibody: Rabbit Anti-HSP27 Polyclonal Antibody (SPC-1264) at 1:1000. Predicted/Observed Size: ~27kDa.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~27kDa HSP27 protein using Rabbit Anti-HSP27 Polyclonal Antibody (SPC-1264). Lane 1 and Lane 2: Human HeLa untreated or treated with Ca2+, Hsp27 Antibody (Ser82). Lane 3 and Lane 4: Human HeLa untreated or treated with Ca2+, Hsp27 Antibody (pSer82). Primary Antibody: Rabbit Anti-HSP27 Polyclonal Antibody (SPC-1264) at 1:1000. Predicted/Observed Size: ~27kDa.
quantity: 100 µl
price: 260 USD
to the supplier