product summary
company name :
St John's Laboratory
product type :
antibody
product name :
Anti-Rad51 antibody
catalog :
STJ99103
quantity :
100μl
price :
150 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4H10-G5-H4
reactivity :
human
application :
western blot, ELISA
image
image 1 :
St John's Laboratory STJ99103 image 1
Western blot detection of Rad51 in CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Rad51 mouse mAb (1:1000 diluted). Predicted band size: 37KDa. Observed band size: 37KDa.
product information
Catalog Number :
STJ99103
Product Name :
Anti-Rad51 antibody
Product Type :
Primary Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugation :
Unconjugated
Clone Name :
4H10-G5-H4
Isotype :
IgG2A
Size :
100μl
List Price :
150 USD
Product Description :
Rad51 is a protein encoded by the RAD51 gene which is approximately 35 kDa. Rad51 is localised to the nucleus, cytoplasm and mitochondrion matirx. It is involved in the resolution of D-loop structures through synthesis-dependent strand annealing, DNA double-strand break repair, ATM pathway and the BRCA1 pathway. It is known to be involved in the homologous strand exchange, a key step in DNA repair through homologous recombination and repair of DNA. It catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Rad51 is highly expressed in the testis and thymus and less expressed in the small intestine, placenta, colon, pancreas and ovary. Mutations in the RAD51 gene result in breast cancer and Fanconi anemia, a disorder affecting all bone marrow elements. STJ99103 was developed from clone 4H10-G5-H4. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and detects endogenous levels of Rad51 and does not cross-react with related proteins.
CrossReactivity :
Human, Mouse, Rat, Monkey
ALTnames :
RAD51 antibody, RAD51A antibody, RECA antibody, DNA repair protein RAD51 homolog 1 antibody, HsRAD51 antibody, hRAD51 antibody, RAD51 homolog A antibody
Immunogen :
Recombinant peptide derived from human Rad51.
Localization :
Nucleus Cytoplasm Cytoplasm, perinuclear region. Mitochondrion matrix Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
Specificity :
This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.
Purity :
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer :
Liquid in PBS containing 50% glycerol, 0.5% BSA
Preservative :
0.02% sodium azide
Concentration :
1 mg/ml
Application Summary :
WB, ELISA
Storage :
Store at -20°C, and avoid repeat freeze-thaw cycles.
company information
St John's Laboratory
Unit 31, 35 Evelyn Street
London, SE8 5RT
info@stjohnslabs.com
www.stjohnslabs.com
+44 (0)2072322321
headquarters: UK