Anti-HIF-1α antibody | St John's Laboratory STJ93498 product information

product summary

company name :

St John's Laboratory

product type :

antibody

product name :

Anti-HIF-1α antibody

catalog :

STJ93498

quantity :

100μl

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

St John's Laboratory product webpage

image

image 1 :

Western blot (WB) analysis of HIF-1alpha Polyclonal Antibody.

product information

Catalog Number :

STJ93498

Product Name :

Anti-HIF-1α antibody

Product Type :

Primary Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Isotype :

IgG

Size :

100μl

List Price :

150 USD

Product Description :

HIF-1α is a protein encoded by the HIF1A gene which is approximately 92, 6 kDa. HIF-1α is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation, removal of Cdc6, HIF repressor pathways, signalling by PTK6 and the notch signalling pathway. It functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1α is expressed in most tissues with highest levels in kidney and heart. Mutations in the HIF1A gene may result in hypoxia and retinal ischemia. STJ93498 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of HIF-1α protein.

CrossReactivity :

Human, Mouse, Rat

ALTnames :

HIF1A antibody, BHLHE78 antibody, MOP1 antibody, PASD8 antibody, Hypoxia-inducible factor 1-alpha antibody, HIF-1-alpha antibody, HIF1-alpha antibody, ARNT-interacting protein antibody, Basic-helix-loop-helix-PAS protein MOP1 antibody, Class E basic helix-loop-helix protein 78 antibody, bHLHe78 antibody, Member of PAS protein 1 antibody, PAS domain-containing protein 8 antibody

Immunogen :

Synthesized peptide derived from human HIF-1α.

Epitope :

Internal

Localization :

Cytoplasm Nucleus Nucleus speckle Note=Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia .

Specificity :

HIF-1α Polyclonal Antibody detects endogenous levels of HIF-1α protein.

Purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer :

Liquid in PBS containing 50% glycerol, 0.5% BSA

Preservative :

0.02% sodium azide

Concentration :

1 mg/ml

Application Summary :

WB, IHC-p, ELISA

Storage :

Store at -20°C, and avoid repeat freeze-thaw cycles.

more info or order :

St John's Laboratory product webpage