Anti-Phospho-IκB-α (Y305) antibody | St John's Laboratory STJ90912 product information

product summary

company name :

St John's Laboratory

product type :

antibody

product name :

Anti-Phospho-IκB-α (Y305) antibody

catalog :

STJ90912

quantity :

100μl

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

St John's Laboratory product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°,overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

product information

Catalog Number :

STJ90912

Product Name :

Anti-Phospho-IκB-α (Y305) antibody

Product Type :

Primary Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Antigen Modification :

phosphorylated

Isotype :

IgG

Size :

100μl

List Price :

150 USD

Product Description :

IκB-α is a protein encoded by the NFKBIA gene which is approximately 35,6 kDa. IκB-α is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, the 4-1BB pathway and toll-like receptor signalling pathways. This protein falls under the NF-kappa-B inhibitor family. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals, this process is involved in inflammatory responses. IκB-α is induced in adherent monocytes. Mutations in the NFKBIA gene may result in ectodermal dysplasia with T-cell immunodeficiency. STJ90912 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IκB-α protein which only binds about Y305 when Y305 is phosphorylated.

CrossReactivity :

Human, Mouse, Rat, Monkey

ALTnames :

NFKBIA antibody, IKBA antibody, MAD3 antibody, NFKBI antibody, NF-kappa-B inhibitor alpha antibody, I-kappa-B-alpha antibody, IkB-alpha antibody, IkappaBalpha antibody, Major histocompatibility complex enhancer-binding protein MAD3 antibody, Phospho-IκB-α (Y305) antibody

Immunogen :

Synthesized peptide derived from human IκB-α around the phosphorylation site of Y305.

Epitope :

240-320 aa

Localization :

Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Specificity :

Phospho-IκB-α (Y305) Polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at Y305.

Purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer :

Liquid in PBS containing 50% glycerol, 0.5% BSA

Preservative :

0.02% sodium azide

Concentration :

1 mg/ml

Application Summary :

WB, IHC-p, ELISA

Storage :

Store at -20°C, and avoid repeat freeze-thaw cycles.

more info or order :

St John's Laboratory product webpage