Anti-Phospho-p70 S6 kinase α (S447) antibody | St John's Laboratory STJ90374 product information

product summary

company name :

St John's Laboratory

product type :

antibody

product name :

Anti-Phospho-p70 S6 kinase α (S447) antibody

catalog :

STJ90374

quantity :

100μl

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

St John's Laboratory product webpage

image

image 1 :

Western Blot (WB) analysis of specific cells using Phospho-p70 S6 kinase alpha (S447) Polyclonal Antibody.

product information

Catalog Number :

STJ90374

Product Name :

Anti-Phospho-p70 S6 kinase α (S447) antibody

Product Type :

Primary Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Antigen Modification :

phosphorylated

Isotype :

IgG

Size :

100μl

List Price :

150 USD

Product Description :

Phospho-p70 S6 kinase alpha is a protein encoded by the RPS6KB1 gene which is approximately 59,2 kDa. Phospho-p70 S6 kinase alpha is localised to the mitochondrion and cell junctions. It is involved in pathways such as the common cytokine receptor gamma-c family signalling pathway, regulation of lipid metabolism insulin signalling-generic cascades, RET signalling and mTOR signalling. This protein falls under the ribosomal S6 kinase family of serine/threonine kinases. It responds to mTOR signalling and is activated in response to growth factors and nutrients to promote protein synthesis, cell growth, cell cycle progression and cell proliferation. It regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Phospho-p70 S6 kinase alpha is expressed in the cells of the eye, nervous system, muscle, heart and liver. Mutations in the RPS6KB1 gene result in diseases such as tuberous sclerosis which manifests as hamartoma formation in multiple organ systems and glioblastoma multiforme which is a central nervous system tumour of grade IV histological malignancy. STJ90374 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of S447. This primary antibody specifically binds to endogenous p70 S6 kinase α protein which only binds about S447 when S447 is phosphorylated.

CrossReactivity :

Human, Mouse, Rat

ALTnames :

RPS6KB1 antibody, STK14A antibody, Ribosomal protein S6 kinase beta-1 antibody, S6K-beta-1 antibody, S6K1 antibody, 70 kDa ribosomal protein S6 kinase 1 antibody, P70S6K1 antibody, p70-S6K 1 antibody, Ribosomal protein S6 kinase I antibody, Serine/threonine-protein kinase 14A antibody, p70 ribosomal S6 kinase alpha antibody, p70 S6 kinase alpha antibody, p70 S6K-alpha antibody, p70 S6KA antibody, Phospho-p70 S6 kinase α (S447) antibody

Immunogen :

Synthesized peptide derived from human p70 S6 kinase α around the phosphorylation site of S447.

Epitope :

390-470 aa

Localization :

Cell junction, synapse, synaptosome Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion.; Isoform Alpha I: Nucleus. Cytoplasm.; Isoform Alpha II: Cytoplasm.

Specificity :

Phospho-p70 S6 kinase α (S447) Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein only when phosphorylated at S447.

Purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer :

Liquid in PBS containing 50% glycerol, 0.5% BSA

Preservative :

0.02% sodium azide

Concentration :

1 mg/ml

Application Summary :

WB, IHC-p, ELISA

Storage :

Store at -20°C, and avoid repeat freeze-thaw cycles.

more info or order :

St John's Laboratory product webpage