Anti-Phospho-Akt1 (T450) antibody | St John's Laboratory STJ90168 product information

product summary

company name :

St John's Laboratory

product type :

antibody

product name :

Anti-Phospho-Akt1 (T450) antibody

catalog :

STJ90168

quantity :

100μl

price :

150 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

St John's Laboratory product webpage

image

image 1 :

Western Blot (WB) analysis of specific lysis using Phospho-Akt1 (T450) Polyclonal Antibody.

product information

Catalog Number :

STJ90168

Product Name :

Anti-Phospho-Akt1 (T450) antibody

Product Type :

Primary Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Antigen Modification :

phosphorylated

Isotype :

IgG

Size :

100μl

List Price :

150 USD

Product Description :

Akt1 is a protein encoded by the oncogene AKT1 which is approximately 55,7 kDa. Akt1 is localised to the cytoplasma, nucleus and cell membrane. It is involved in RET signalling, the regulation of lipid metabolism, insulin signalling-generic cascades, common cytokine receptor gamma-chain family signalling pathways and apoptotic pathways. This protein falls under the AKT kinase family. The AKT1 isoform has a specific role in cell motility and proliferation. By activating serine/threonine kinase AKT1 apoptosis can be suppressed by phosphorylating and therby inactivating components of the apoptotic machinery. Mutations in the AKT1 gene can result in breast cancer, colorectal cancer, Cowden syndrome 6 and Proteus syndrome which causes overgrowth. STJ90168 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Akt1 protein which only binds about T450 when T450 is phosphorylated.

CrossReactivity :

Human, Mouse, Rat

ALTnames :

AKT1 antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, Phospho-Akt1 (T450) antibody

Immunogen :

Synthesized peptide derived from human Akt1 around the phosphorylation site of T450.

Epitope :

390-470 aa

Localization :

Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles .

Specificity :

Phospho-Akt1 (T450) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450.

Purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer :

Liquid in PBS containing 50% glycerol, 0.5% BSA

Preservative :

0.02% sodium azide

Concentration :

1 mg/ml

Application Summary :

WB, IHC-p, ELISA

Storage :

Store at -20°C, and avoid repeat freeze-thaw cycles.

more info or order :

St John's Laboratory product webpage