SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified | Sino Biological 40592-T62 product information

product summary

company name :

Sino Biological

product type :

antibody

product name :

SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified

catalog :

40592-T62

quantity :

100 µL

price :

299 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

SARS-CoV-2, SARS coronavirus, domestic rabbit

application :

western blot, ELISA

more info or order :

Sino Biological product webpage

citations: 59

Published Application/Species/Sample/Dilution	Reference
ELISA; SARS coronavirus western blot; SARS coronavirus	Kim J, Kim S, Park S, Kim D, Kim M, Baek K, et al. Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance. Microbes Infect. 2024;:105461 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Ojha R, Jiang A, M xe4 ntyl xe4 E, Quirin T, Modhira N, Witte R, et al. Dynamin independent endocytosis is an alternative cell entry mechanism for multiple animal viruses. PLoS Pathog. 2024;20:e1012690 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Huang J, Wang W, Li H, Bai Y, Song Y, Jiao C, et al. Three in one: An effective and universal vaccine expressing heterologous tandem RBD trimer by rabies virus vector protects mice against SARS-CoV-2. Antiviral Res. 2024;227:105905 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zhang Q, Tiwari S, Wen J, Wang S, Wang L, Li W, et al. Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domains in mice. PLoS ONE. 2024;19:e0300524 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Xing M, Hu G, Wang X, Wang Y, He F, Dai W, et al. An intranasal combination vaccine induces systemic and mucosal immunity against COVID-19 and influenza. NPJ Vaccines. 2024;9:64 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Roy A, Chan M, Banadyga L, He S, Zhu W, Chr xe9 tien M, et al. Quercetin inhibits SARS-CoV-2 infection and prevents syncytium formation by cells co-expressing the viral spike protein and human ACE2. Virol J. 2024;21:29 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Lusvarghi S, Stauft C, Vassell R, Williams B, Baha H, Wang W, et al. Effects of N-glycan modifications on spike expression, virus infectivity, and neutralization sensitivity in ancestral compared to Omicron SARS-CoV-2 variants. PLoS Pathog. 2023;19:e1011788 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Li Y, Mo J, Liu J, Liang Y, Deng C, Huang Z, et al. A micro-electroporation/electrophoresis-based vaccine screening system reveals the impact of vaccination orders on cross-protective immunity. iScience. 2023;26:108086 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Wang Z, Pan Q, Ma L, Zhao J, McIntosh F, Liu Z, et al. Anthracyclines inhibit SARS-CoV-2 infection. Virus Res. 2023;334:199164 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	McConnell S, Sachithanandham J, Mudrak N, Zhu X, Farhang P, Cordero R, et al. Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralization. Cell Chem Biol. 2023;30:726-738.e4 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zhang Y, Liu J, Li H, Yuan F, Jiang C, Cang T, et al. Comparison of the immunogenicity of nasal-spray rVSV vector, adenovirus vector, and inactivated COVID-19-based vaccines in rodent models. J Med Virol. 2023;95:e28806 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Sormunen A, Koivulehto E, Alitalo K, Saksela K, Laham Karam N, Yl xe4 Herttuala S. Comparison of Automated and Traditional Western Blotting Methods. Methods Protoc. 2023;6: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Xia S, Jiao F, Wang L, Yu X, Lu T, Fu Y, et al. SARS-CoV-2 Omicron XBB subvariants exhibit enhanced fusogenicity and substantial immune evasion in elderly population, but high sensitivity to pan-coronavirus fusion inhibitors. J Med Virol. 2023;95:e28641 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Ham Y, Cho N, Kim D, Kim J, Jo M, Jeong M, et al. The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction. Biosens Bioelectron. 2023;227:115169 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Azevedo P, Hojo Souza N, Faustino L, Fumagalli M, Hirako I, Oliveira E, et al. Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concern. NPJ Vaccines. 2023;8:15 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Radovic S, Meng W, Chen L, Paniz Mondolfi A, Bryce C, Grimes Z, et al. SARS-CoV-2 infection of kidney tissues from severe COVID-19 patients. J Med Virol. 2023;95:e28566 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Xia S, Wang L, Jiao F, Yu X, Xu W, Huang Z, et al. SARS-CoV-2 Omicron subvariants exhibit distinct fusogenicity, but similar sensitivity, to pan-CoV fusion inhibitors. Emerg Microbes Infect. 2023;12:2178241 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zhang H, Jin H, Yan F, Song Y, Dai J, Jiao C, et al. An inactivated recombinant rabies virus chimerically expressed RBD induces humoral and cellular immunity against SARS-CoV-2 and RABV. Virol Sin. 2023;38:244-256 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zhao H, Wang M, Muthelo P, L xf6 f L, Sterky F, Gallini R, et al. Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay. N Biotechnol. 2022;72:139-148 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Pszenny V, Tjhin E, Alves Ferreira E, Spada S, Bouamr F, Nair V, et al. Using the Sleeping Beauty (SB) Transposon to Generate Stable Cells Producing Enveloped Virus-Like Particles (eVLPs) Pseudotyped with SARS-CoV-2 Proteins for Vaccination. Curr Protoc. 2022;2:e575 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Meng W, Guo S, Cao S, Shuda M, Robinson McCarthy L, McCarthy K, et al. Development and characterization of a new monoclonal antibody against SARS-CoV-2 NSP12 (RdRp). J Med Virol. 2022;: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Ao Z, Ouyang M, Olukitibi T, Yao X. SARS-CoV-2 Delta spike protein enhances the viral fusogenicity and inflammatory cytokine production. iScience. 2022;25:104759 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zhang Q, Tiwari S, Wang S, Wang L, Li W, Zhang L, et al. Induction of neutralizing antibodies against SARS-CoV-2 variants by a multivalent mRNA-lipid nanoparticle vaccine encoding SARS-CoV-2/SARS-CoV Spike protein receptor-binding domains. bioRxiv. 2022;: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Zabaleta N, Bhatt U, H xe9 rate C, Maisonnasse P, Sanmiguel J, Diop C, et al. Durable immunogenicity, adaptation to emerging variants, and low-dose efficacy of an AAV-based COVID-19 vaccine platform in macaques. Mol Ther. 2022;30:2952-2967 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Slattery S, Giguere D, Stuckless E, Shrestha A, Briere L, Galbraith A, et al. Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics. Sci Rep. 2022;12:7010 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Badeti S, Jiang Q, Naghizadeh A, Tseng H, Bushkin Y, Marras S, et al. Development of a Novel Human CD147 Knock-in NSG Mouse Model to Test SARS-CoV-2 Viral Infection. Res Sq. 2022;: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Singh P, Mukherji S, Basak S, Hoffmann M, Das D. Dynamic Ca2+ sensitivity stimulates the evolved SARS-CoV-2 spike strain-mediated membrane fusion for enhanced entry. Cell Rep. 2022;39:110694 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Medigeshi G, Batra G, Murugesan D, Thiruvengadam R, Chattopadhyay S, Das B, et al. Sub-optimal neutralisation of omicron (B.1.1.529) variant by antibodies induced by vaccine alone or SARS-CoV-2 Infection plus vaccine (hybrid immunity) post 6-months. EBioMedicine. 2022;78:103938 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus ELISA; domestic rabbit western blot; domestic rabbit	Yang F, Liu L, NEUENSCHWANDER P, Idell S, Vankayalapati R, Jain K, et al. Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2. ACS Omega. 2022;7:3203-3211 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Son J, Huang S, Zeng Q, Bricker T, Case J, Zhou J, et al. JIB-04 Has Broad-Spectrum Antiviral Activity and Inhibits SARS-CoV-2 Replication and Coronavirus Pathogenesis. MBio. 2022;:e0337721 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Ding L, Zhang Y, Wen D, Ma J, Yuan H, Li H, et al. Growth, Antigenicity, and Immunogenicity of SARS-CoV-2 Spike Variants Revealed by a Live rVSV-SARS-CoV-2 Virus. Front Med (Lausanne). 2021;8:793437 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus ELISA; domestic rabbit western blot; domestic rabbit	Sung J, Liu Y, Wu K, Choi M, Ma C, Lin J, et al. Expression of SARS-CoV-2 Spike Protein Receptor Binding Domain on Recombinant B. subtilis on Spore Surface: A Potential COVID-19 Oral Vaccine Candidate. Vaccines (Basel). 2021;10: pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Wang Y, Wu Y, Wang Q, Zhu J, Shi W, Han Z, et al. Virucidal effect of povidone-iodine against SARS-CoV-2 in vitro. J Int Med Res. 2021;49:3000605211063695 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Kim G, Choi J, Wu K, Saeedian N, Yang E, Park H, et al. A vesicular stomatitis virus-based prime-boost vaccination strategy induces potent and protective neutralizing antibodies against SARS-CoV-2. PLoS Pathog. 2021;17:e1010092 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Dowall S, Salguero F, Wiblin N, Fotheringham S, Hatch G, Parks S, et al. Development of a Hamster Natural Transmission Model of SARS-CoV-2 Infection. Viruses. 2021;13: pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Berndt A, Smalley T, Ren B, Simkovsky R, Badary A, Sproles A, et al. Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii. PLoS ONE. 2021;16:e0257089 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Chen J, Wang B, Caserto J, Shariati K, Cao P, Pan Y, et al. Sustained Delivery of SARS-CoV-2 RBD Subunit Vaccine Using a High Affinity Injectable Hydrogel Scaffold. Adv Healthc Mater. 2021;:e2101714 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Lam B, Kung Y, Lin J, Tseng S, Tsai Y, He L, et al. In vivo characterization of emerging SARS-CoV-2 variant infectivity and human antibody escape potential. Cell Rep. 2021;37:109838 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Wu K, Chugh V, D Krishna V, Di Girolamo A, Wang Y, Saha R, et al. One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase. ACS Appl Mater Interfaces. 2021;13:44136-44146 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Puthenveetil R, Lun C, Murphy R, Healy L, Vilmen G, Christenson E, et al. S-acylation of SARS-CoV-2 spike protein: Mechanistic dissection, in vitro reconstitution and role in viral infectivity. J Biol Chem. 2021;297:101112 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Lu M, Zhang Y, Dravid P, Li A, Zeng C, Kc M, et al. A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters. J Virol. 2021;95:e0059221 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Nguyen N, Kim S, Lindemann G, Berry V. COVID-19 Spike Protein Induced Phononic Modification in Antibody-Coupled Graphene for Viral Detection Application. ACS Nano. 2021;15:11743-11752 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Ao Z, Chan M, Ouyang M, Olukitibi T, Mahmoudi M, Kobasa D, et al. Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry. PLoS ONE. 2021;16:e0251649 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Seephetdee C, Buasri N, Bhukhai K, Srisanga K, Manopwisedjaroen S, Lertjintanakit S, et al. Mice Immunized with the Vaccine Candidate HexaPro Spike Produce Neutralizing Antibodies against SARS-CoV-2. Vaccines (Basel). 2021;9: pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Watanabe Y, Mendonça L, Allen E, Howe A, Lee M, Allen J, et al. Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine. ACS Cent Sci. 2021;7:594-602 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Chai K, Tzeng T, Shen K, Liao H, Lin J, Chen M, et al. DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. PLoS Negl Trop Dis. 2021;15:e0009374 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Luo S, Zhang P, Liu B, Yang C, Liang C, Wang Q, et al. Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates. Emerg Microbes Infect. 2021;10:1002-1015 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Park B, Kim J, Park S, Kim D, Kim M, Baek K, et al. MERS-CoV and SARS-CoV-2 replication can be inhibited by targeting the interaction between the viral spike protein and the nucleocapsid protein. Theranostics. 2021;11:3853-3867 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Rusanen J, Kareinen L, Levanov L, Mero S, Pakkanen S, Kantele A, et al. A 10-Minute "Mix and Read" Antibody Assay for SARS-CoV-2. Viruses. 2021;13: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Liu R, Americo J, Cotter C, Earl P, Erez N, Peng C, et al. MVA Vector Vaccines Inhibit SARS CoV-2 Replication in Upper and Lower Respiratory Tracts of Transgenic Mice and Prevent Lethal Disease. bioRxiv. 2021;: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus ELISA; domestic rabbit western blot; domestic rabbit	Zang R, Case J, Yutuc E, Ma X, Shen S, Gomez Castro M, et al. Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion. Proc Natl Acad Sci U S A. 2020;117:32105-32113 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus; 1:1000; loading ...; fig s3f ELISA; domestic rabbit western blot; domestic rabbit	Daly J, Simonetti B, Klein K, Chen K, Williamson M, Antón Plágaro C, et al. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science. 2020;370:861-865 pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus ELISA; domestic rabbit western blot; domestic rabbit	McAndrews K, Dowlatshahi D, Dai J, Becker L, Hensel J, Snowden L, et al. Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity. JCI Insight. 2020;5: pubmed publisher
ELISA; SARS coronavirus western blot; SARS coronavirus	Cai Y, Zhang J, Xiao T, Peng H, Sterling S, Walsh R, et al. Distinct conformational states of SARS-CoV-2 spike protein. Science. 2020;369:1586-1592 pubmed publisher
ELISA; domestic rabbit western blot; domestic rabbit ELISA; SARS coronavirus western blot; SARS coronavirus	Yu J, Tostanoski L, Peter L, Mercado N, McMahan K, Mahrokhian S, et al. DNA vaccine protection against SARS-CoV-2 in rhesus macaques. Science. 2020;: pubmed publisher
	Khan I, Li S, Tao L, Wang C, Ye B, Li H, et al. Tubeimosides are pan-coronavirus and filovirus inhibitors that can block their fusion protein binding to Niemann-Pick C1. Nat Commun. 2024;15:162 pubmed publisher
	Weklak D, Tisborn J, Mangold M, Scheu R, Wodrich H, Hagedorn C, et al. Insights from the Construction of Adenovirus-Based Vaccine Candidates against SARS-CoV-2: Expecting the Unexpected. Viruses. 2023;15: pubmed publisher
	Ma Y, Wang Y, Dong C, Gonzalez G, Zhu W, Kim J, et al. SARS-CoV-2 Spike Stem Protein Nanoparticles Elicited Broad ADCC and Robust Neutralization against Variants in Mice. Small. 2022;18:e2200836 pubmed publisher
	Brune K, Lieknina I, Sutov G, Morris A, Jovicevic D, Kalnins G, et al. N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone. Chembiochem. 2021;22:3199-3207 pubmed publisher

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image 1 :

product information

Catalog Number :

40592-T62

Product Name :

SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified

Product Type :

Antibody

Host Species :

Rabbit

Clonality :

polyclonal

Conjugation :

Unconjugated

Isotype :

IgG

Size :

100 µL

List Price :

299 USD

Product Description :

Produced in rabbits immunized with purified, recombinant SARS-CoV-2 / 2019-nCoV Spike/RBD Protein (YP_009724390.1; Arg319-Phe541). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Spike/RBD affinity chromatography.

SpeciesSummary :

SARS-CoV-2

ALTnames :

Spike

Immunogen :

Recombinant SARS-CoV-2 / 2019-nCoV Spike/RBD Protein

Purity :

Protein A & Antigen Affinity

Buffer :

0.2 μm filtered solution in PBS

Application Summary :

WB,ELISA

Storage :

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

more info or order :

Sino Biological product webpage