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company name :
Sino Biological
product type :
protein
product name :
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein
catalog :
40589-V08B1
quantity :
100 µg
price :
498 USD
more info or order :
citations: 93
Published Application/Species/Sample/DilutionReference
  • ELISA; SARS coronavirus
Acharya P, Williams W, Henderson R, Janowska K, Manne K, Parks R, et al. A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies. bioRxiv. 2020;: pubmed publisher
  • ELISA; SARS coronavirus
Yee J, Van Rompay K, Carpenter A, Nham P, Halley B, Iyer S, et al. SARS-CoV-2 surveillance for a non-human primate breeding research facility. J Med Primatol. 2020;: pubmed publisher
  • ELISA; SARS coronavirus
Lei H, Ye F, Liu X, Huang Z, Ling S, Jiang Z, et al. SARS-CoV-2 environmental contamination associated with persistently infected COVID-19 patients. Influenza Other Respir Viruses. 2020;: pubmed publisher
Wickenhagen A, Flagg M, Port J, Yinda C, Goldin K, Gallogly S, et al. Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology. Nat Commun. 2025;16:594 pubmed publisher
Zhang Z, Zhang Y, Cheng L, Zhang L, Yan Q, Liu X, et al. Defining the features and structure of neutralizing antibody targeting the silent face of the SARS-CoV-2 spike N-terminal domain. MedComm (2020). 2024;5:e70008 pubmed publisher
Deng W, Hu X, Tian X, Zhang Y, Shang W, Zhang L, et al. Peptidomimetic Analogues Act as Effective Inhibitors against SARS-CoV-2 by Blocking the Function of Cathepsin L. J Med Chem. 2024;67:17124-17143 pubmed publisher
Hud xe1 k A, Pusztai D, Letoha A, Letoha T. Mutual Inhibition of Antithrombin III and SARS-CoV-2 Cellular Attachment to Syndecans: Implications for COVID-19 Treatment and Vaccination. Int J Mol Sci. 2024;25: pubmed publisher
Geanes E, McLennan R, Pierce S, Menden H, Paul O, Sampath V, et al. SARS-CoV-2 envelope protein regulates innate immune tolerance. iScience. 2024;27:109975 pubmed publisher
Khalil B, Sharif Askari N, Hafezi S, Sharif Askari F, Al Anouti F, Hamid Q, et al. Vitamin D regulates COVID-19 associated severity by suppressing the NLRP3 inflammasome pathway. PLoS ONE. 2024;19:e0302818 pubmed publisher
Liu R, Natekar J, Kim K, Pathak H, Bhatnagar N, Raha J, et al. Multivalent and Sequential Heterologous Spike Protein Vaccinations Effectively Induce Protective Humoral Immunity against SARS-CoV-2 Variants. Vaccines (Basel). 2024;12: pubmed publisher
Prakash S, Dhanushkodi N, Singer M, Quadiri A, Zayou L, Vahed H, et al. A Broad-Spectrum Multi-Antigen mRNA/LNP-Based Pan-Coronavirus Vaccine Induced Potent Cross-Protective Immunity Against Infection and Disease Caused by Highly Pathogenic and Heavily Spike-Mutated SARS-CoV-2 Variants of Concern in the Syrian Hamster Mo. bioRxiv. 2024;: pubmed publisher
Bae M, Choi S, Kim J, Seo G, Lee Y. Temperature-insensitive label-free SARS-CoV-2 spike protein detection based on complementary refractive index and temperature dependence of multi-mode interference and grating resonance. Talanta. 2024;266:125091 pubmed publisher
Zak A, Hoang T, Yee C, Rizvi S, Prabhu P, Wen F. Pseudotyping Improves the Yield of Functional SARS-CoV-2 Virus-like Particles (VLPs) as Tools for Vaccine and Therapeutic Development. Int J Mol Sci. 2023;24: pubmed publisher
Chiuppesi F, Ortega Francisco S, Gutiérrez M, Li J, Ly M, Faircloth K, et al. Stimulation of Potent Humoral and Cellular Immunity via Synthetic Dual-Antigen MVA-Based COVID-19 Vaccine COH04S1 in Cancer Patients Post Hematopoietic Cell Transplantation and Cellular Therapy. Vaccines (Basel). 2023;11: pubmed publisher
Liang T, Xiao S, Wu Z, Lv X, Liu S, Hu M, et al. Phenothiazines Inhibit SARS-CoV-2 Entry through Targeting Spike Protein. Viruses. 2023;15: pubmed publisher
Morita R, Kubota Koketsu R, Lu X, Sasaki T, Nakayama E, Liu Y, et al. COVID-19 relapse associated with SARS-CoV-2 evasion from CD4+ T-cell recognition in an agammaglobulinemia patient. iScience. 2023;26:106685 pubmed publisher
Wussow F, Kha M, Kim T, Ly M, Yll Pico M, Kar S, et al. Synthetic multiantigen MVA vaccine COH04S1 and variant-specific derivatives protect Syrian hamsters from SARS-CoV-2 Omicron subvariants. NPJ Vaccines. 2023;8:41 pubmed publisher
Jia T, Wu Y, Liao G, Lei Y, Wu Z, Yang F, et al. Stable and durable antibody responses in SARS-recovered donors vaccinated with inactivated SARS-CoV-2 vaccine. J Med Virol. 2023;95:e28662 pubmed publisher
Palestra F, Poto R, Ciardi R, Opromolla G, Secondo A, Tedeschi V, et al. SARS-CoV-2 Spike Protein Activates Human Lung Macrophages. Int J Mol Sci. 2023;24: pubmed publisher
Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, et al. Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge. Biomaterials. 2023;292:121907 pubmed publisher
Kiyan Y, Schultalbers A, Chernobrivaia E, Tkachuk S, Rong S, Shushakova N, et al. Calcium dobesilate reduces SARS-CoV-2 entry into endothelial cells by inhibiting virus binding to heparan sulfate. Sci Rep. 2022;12:16878 pubmed publisher
Oh C, Nakamura T, Beutler N, Zhang X, Pi xf1 a Crespo J, Talantova M, et al. Targeted protein S-nitrosylation of ACE2 inhibits SARS-CoV-2 infection. Nat Chem Biol. 2022;: pubmed publisher
Ebenig A, Muraleedharan S, Kazmierski J, Todt D, Auste A, Anzaghe M, et al. Vaccine-associated enhanced respiratory pathology in COVID-19 hamsters after TH2-biased immunization. Cell Rep. 2022;40:111214 pubmed publisher
Han J, Lee S, Kim J, Seo G, Lee Y. SARS-CoV-2 spike protein detection using slightly tapered no-core fiber-based optical transducer. Mikrochim Acta. 2022;189:321 pubmed publisher
Peng L, Fang Z, Renauer P, McNamara A, Park J, Lin Q, et al. Multiplexed LNP-mRNA vaccination against pathogenic coronavirus species. Cell Rep. 2022;40:111160 pubmed publisher
Chiuppesi F, Zaia J, Faircloth K, Johnson D, Ly M, Karpinski V, et al. Vaccine-induced spike- and nucleocapsid-specific cellular responses maintain potent cross-reactivity to SARS-CoV-2 Delta and Omicron variants. iScience. 2022;25:104745 pubmed publisher
Sun X, Yi C, Zhu Y, Ding L, Xia S, Chen X, et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2. Nat Microbiol. 2022;7:1063-1074 pubmed publisher
Gregoire C, Spinelli L, Villazala Merino S, Gil L, Holgado M, Moussa M, et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity. 2022;55:1216-1233.e9 pubmed publisher
Song Y, Zhang H, Zhu Y, Zhao X, Lei Y, Zhou W, et al. Lysozyme Protects Against Severe Acute Respiratory Syndrome Coronavirus 2 Infection and Inflammation in Human Corneal Epithelial Cells. Invest Ophthalmol Vis Sci. 2022;63:16 pubmed publisher
Wussow F, Kha M, Faircloth K, Nguyen V, Iniguez A, Martinez J, et al. COH04S1 and beta sequence-modified vaccine protect hamsters from SARS-CoV-2 variants. iScience. 2022;25:104457 pubmed publisher
Ma Y, Wang Y, Dong C, Gonzalez G, Zhu W, Kim J, et al. SARS-CoV-2 Spike Stem Protein Nanoparticles Elicited Broad ADCC and Robust Neutralization against Variants in Mice. Small. 2022;18:e2200836 pubmed publisher
Nakayama E, Kubota Koketsu R, Sasaki T, Suzuki K, Uno K, Shimizu J, et al. Anti-nucleocapsid antibodies enhance the production of IL-6 induced by SARS-CoV-2 N protein. Sci Rep. 2022;12:8108 pubmed publisher
Walters J, Schouest B, Patel A, Reuschel E, Schultheis K, Parzych E, et al. Prime-boost vaccination regimens with INO-4800 and INO-4802 augment and broaden immune responses against SARS-CoV-2 in nonhuman primates. Vaccine. 2022;40:2960-2969 pubmed publisher
Wen K, Bai C, Zhao H, Zhou P, Gao S, Guan H, et al. Low dose radiation therapy attenuates ACE2 depression and inflammatory cytokines induction by COVID-19 viral spike protein in human bronchial epithelial cells. Int J Radiat Biol. 2022;98:1532-1541 pubmed publisher
Li C, Lee A, Grigoryan L, Arunachalam P, Scott M, Trisal M, et al. Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine. Nat Immunol. 2022;23:543-555 pubmed publisher
Wu P, Yang Q, Zhao X, Liu Q, Xi J, Zhang F, et al. A SARS-CoV-2 nanobody that can bind to the RBD region may be used for treatment in COVID-19 in animals. Res Vet Sci. 2022;145:46-49 pubmed publisher
Li M, Liu J, Lu R, Zhang Y, Du M, Xing M, et al. Longitudinal immune profiling reveals dominant epitopes mediating long-term humoral immunity in COVID-19-convalescent individuals. J Allergy Clin Immunol. 2022;149:1225-1241 pubmed publisher
Chiuppesi F, Nguyen V, Park Y, Contreras H, Karpinski V, Faircloth K, et al. Synthetic multiantigen MVA vaccine COH04S1 protects against SARS-CoV-2 in Syrian hamsters and non-human primates. NPJ Vaccines. 2022;7:7 pubmed publisher
Hudák A, Veres G, Letoha A, Szilak L, Letoha T. Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission. Int J Mol Sci. 2022;23: pubmed publisher
Mironov V, Shchugoreva I, Artyushenko P, Morozov D, Borbone N, Oliviero G, et al. Structure- and Interaction-Based Design of Anti-SARS-CoV-2 Aptamers. Chemistry. 2022;28:e202104481 pubmed publisher
Cecon E, Dam J, Jockers R. Detection of SARS-CoV-2 spike protein binding to ACE2 in living cells by TR-FRET. STAR Protoc. 2022;3:101024 pubmed publisher
Frasca D, Reidy L, Romero M, Diaz A, Cray C, Kahl K, et al. The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing. Int J Obes (Lond). 2022;46:427-432 pubmed publisher
Wang W, Li S, Xu X, Yang C, Niu X, Yin S, et al. Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation. Acta Pharmacol Sin. 2022;43:771-780 pubmed publisher
Lassauniere R, Polacek C, Gram G, Frische A, Tingstedt J, Krüger M, et al. Preclinical evaluation of a candidate naked plasmid DNA vaccine against SARS-CoV-2. NPJ Vaccines. 2021;6:156 pubmed publisher
Amanat F, Strohmeier S, Meade P, Dambrauskas N, Muhlemann B, Smith D, et al. Vaccination with SARS-CoV-2 variants of concern protects mice from challenge with wild-type virus. PLoS Biol. 2021;19:e3001384 pubmed publisher
Pan T, Chen R, He X, Yuan Y, Deng X, Li R, et al. Infection of wild-type mice by SARS-CoV-2 B.1.351 variant indicates a possible novel cross-species transmission route. Signal Transduct Target Ther. 2021;6:420 pubmed publisher
Griffin B, Warner B, Chan M, Valcourt E, Tailor N, Banadyga L, et al. Host parameters and mode of infection influence outcome in SARS-CoV-2-infected hamsters. iScience. 2021;24:103530 pubmed publisher
Zhong C, Xia H, Adam A, Wang B, Hajnik R, Liang Y, et al. Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies. NPJ Vaccines. 2021;6:139 pubmed publisher
Li J, Xu D, Wang L, Zhang M, Zhang G, Li E, et al. Glycyrrhizic Acid Inhibits SARS-CoV-2 Infection by Blocking Spike Protein-Mediated Cell Attachment. Molecules. 2021;26: pubmed publisher
Jiang Z, Zhao T, Li C, Li Y, Huang C. 2D MOF-Based Photoelectrochemical Aptasensor for SARS-CoV-2 Spike Glycoprotein Detection. ACS Appl Mater Interfaces. 2021;13:49754-49761 pubmed publisher
Warner B, Santry L, Leacy A, Chan M, Pham P, Vendramelli R, et al. Intranasal vaccination with a Newcastle disease virus-vectored vaccine protects hamsters from SARS-CoV-2 infection and disease. iScience. 2021;24:103219 pubmed publisher
An X, Martinez Paniagua M, Rezvan A, Sefat S, Fathi M, Singh S, et al. Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2. iScience. 2021;24:103037 pubmed publisher
Nagai M, Moriyama M, Ichinohe T. Oral Bacteria Combined with an Intranasal Vaccine Protect from Influenza A Virus and SARS-CoV-2 Infection. MBio. 2021;12:e0159821 pubmed publisher
Stampfer S, Goldwater M, Jew S, Bujarski S, Regidor B, Daniely D, et al. Response to mRNA vaccination for COVID-19 among patients with multiple myeloma. Leukemia. 2021;35:3534-3541 pubmed publisher
Cecon E, Burridge M, Cao L, Carter L, Ravichandran R, Dam J, et al. SARS-COV-2 spike binding to ACE2 in living cells monitored by TR-FRET. Cell Chem Biol. 2021;: pubmed publisher
Garrido C, Hurst J, Lorang C, Aquino J, Rodriguez J, Pfeiffer T, et al. Asymptomatic or mild symptomatic SARS-CoV-2 infection elicits durable neutralizing antibody responses in children and adolescents. JCI Insight. 2021;6: pubmed publisher
Ku Z, Xie X, Hinton P, Liu X, Ye X, Muruato A, et al. Nasal delivery of an IgM offers broad protection from SARS-CoV-2 variants. Nature. 2021;595:718-723 pubmed publisher
Williams W, Meyerhoff R, Edwards R, Li H, Manne K, Nicely N, et al. Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. Cell. 2021;184:2955-2972.e25 pubmed publisher
Garrett M, Galloway J, Chu H, Itell H, Stoddard C, Wolf C, et al. High-resolution profiling of pathways of escape for SARS-CoV-2 spike-binding antibodies. Cell. 2021;184:2927-2938.e11 pubmed publisher
Low J, Vaqueirinho D, Mele F, Foglierini M, Jerak J, Perotti M, et al. Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2. Science. 2021;372:1336-1341 pubmed publisher
Luo S, Zhang P, Liu B, Yang C, Liang C, Wang Q, et al. Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates. Emerg Microbes Infect. 2021;10:1002-1015 pubmed publisher
Guo Y, Huang L, Zhang G, Yao Y, Zhou H, Shen S, et al. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. Nat Commun. 2021;12:2623 pubmed publisher
Harbour J, Lyski Z, Schell J, Thomas A, Messer W, Slifka M, et al. Cellular and Humoral Immune Responses in Mice Immunized with Vaccinia Virus Expressing the SARS-CoV-2 Spike Protein. J Immunol. 2021;206:2596-2604 pubmed publisher
Kalnin K, Plitnik T, Kishko M, Zhang J, Zhang D, Beauvais A, et al. Immunogenicity and efficacy of mRNA COVID-19 vaccine MRT5500 in preclinical animal models. NPJ Vaccines. 2021;6:61 pubmed publisher
Verma S, Joshi C, Silverstein R, He M, Carter E, Mysorekar I. SARS-CoV-2 colonization of maternal and fetal cells of the human placenta promotes alteration of local renin-angiotensin system. Med (N Y). 2021;2:575-590.e5 pubmed publisher
Akhter J, Quéromès G, Pillai K, Kepenekian V, Badar S, Mekkawy A, et al. The Combination of Bromelain and Acetylcysteine (BromAc) Synergistically Inactivates SARS-CoV-2. Viruses. 2021;13: pubmed publisher
Bollavaram K, Leeman T, Lee M, Kulkarni A, Upshaw S, Yang J, et al. Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V. Protein Sci. 2021;30:1131-1143 pubmed publisher
Zhao M, Yang W, Yang F, Zhang L, Huang W, Hou W, et al. Cathepsin L plays a key role in SARS-CoV-2 infection in humans and humanized mice and is a promising target for new drug development. Signal Transduct Target Ther. 2021;6:134 pubmed publisher
Ren W, Zhu Y, Wang Y, Shi H, Yu Y, Hu G, et al. Comparative analysis reveals the species-specific genetic determinants of ACE2 required for SARS-CoV-2 entry. PLoS Pathog. 2021;17:e1009392 pubmed publisher
Frasca D, Reidy L, Cray C, Diaz A, Romero M, Kahl K, et al. Influence of obesity on serum levels of SARS-CoV-2-specific antibodies in COVID-19 patients. PLoS ONE. 2021;16:e0245424 pubmed publisher
Bläckberg A, Fernström N, Sarbrant E, Rasmussen M, Sunnerhagen T. Antibody kinetics and clinical course of COVID-19 a prospective observational study. PLoS ONE. 2021;16:e0248918 pubmed publisher
Cotugno N, Ruggiero A, Bonfante F, Petrara M, Zicari S, Pascucci G, et al. Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies. Cell Rep. 2021;34:108852 pubmed publisher
Bertoglio F, Meier D, Langreder N, Steinke S, Rand U, Simonelli L, et al. SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface. Nat Commun. 2021;12:1577 pubmed publisher
He X, Chandrashekar A, Zahn R, Wegmann F, Yu J, Mercado N, et al. Low-Dose Ad26.COV2.S Protection Against SARS-CoV-2 Challenge in Rhesus Macaques. bioRxiv. 2021;: pubmed publisher
Lu S, Xie X, Zhao L, Wang B, Zhu J, Yang T, et al. The immunodominant and neutralization linear epitopes for SARS-CoV-2. Cell Rep. 2021;34:108666 pubmed publisher
Nunes Santos C, Kuehn H, Rosenzweig S. N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects. J Clin Immunol. 2021;41:335-344 pubmed publisher
Wang D, Baudys J, Bundy J, Solano M, Keppel T, Barr J. Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry. Anal Chem. 2020;92:14730-14739 pubmed publisher
Chang A, Croix M, Kenney P, Baron S, Hicar M. Serum Responses of Children With Kawasaki Disease Against Severe Acute Respiratory Syndrome Coronavirus 2 Proteins. Pediatr Infect Dis J. 2020;39:e366-e367 pubmed publisher
Du S, Cao Y, Zhu Q, Yu P, Qi F, Wang G, et al. Structurally Resolved SARS-CoV-2 Antibody Shows High Efficacy in Severely Infected Hamsters and Provides a Potent Cocktail Pairing Strategy. Cell. 2020;183:1013-1023.e13 pubmed publisher
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image
image 1 :
Sino Biological 40589-V08B1 image 1
product information
Catalog Number :
40589-V08B1
Product Name :
SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein
Product Type :
Protein
Host Species :
Baculovirus-Insect Cells
Conjugation :
C-His
Size :
100 µg
List Price :
498 USD
Gene ID :
YP_009724390.1
Product Description :
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Val 16-Pro1213) was expressed with a polyhistidine tag at the C-terminus.
SpeciesSummary :
SARS-CoV-2
ALTnames :
Spike
Purity :
> 90 % as determined by SDS-PAGE.
Buffer :
Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Packaging :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
more info or order :
company information
Sino Biological
Building 9, No.18 Kechuang 10th St, BDA, Beijing, 100176
marketing@sinobiological.com
https://www.sinobiological.com
86 400-890-9989
headquarters: China
Sino Biological is an international reagent supplier and service provider. The company specializes in recombinant protein production and antibody development. All of Sino Biological's products are independently developed and produced, including recombinant proteins, antibodies and cDNA clones. Sino Biological is the researchers' one-stop technical services shop for the advanced technology platforms they need to make advancements. In addition, Sino Biological offer pharmaceutical companies and biotechnology firms pre-clinical production technology services for hundreds of monoclonal antibody drug candidates.