protein

Human CLDN9 -Nanodisc Protein(His & AVI Tag), Biotinylated

21185-H49H-NA-B

100 µg

2198 USD

Human CLDN9 -Nanodisc Protein(His & AVI Tag), Biotinylated

Protein

HEK293 Cells

C-His-AVI

100 µg

2198 USD

O95484

A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.

Human

DFNB116

>80% as determined by SDS-PAGE.

Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.

It is supplied and shipped as liquid with dry ice.

Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.