| Published Application/Species/Sample/Dilution | Reference |
|---|
| Huang R, Spliedt M, Kaufman T, Gorlatov S, Barat B, Shah K, et al. A Strategy for Simultaneous Engineering of Interspecies Cross-Reactivity, Thermostability, and Expression of a Bispecific 5T4 x CD3 DART® Molecule for Treatment of Solid Tumors. Antibodies (Basel). 2025;14: pubmed publisher |
| Abba Moussa D, Vázquez M, Chable Bessia C, Roux Portalez V, Tamagnini E, Pedotti M, et al. Discovery of a pan anti-SARS-CoV-2 monoclonal antibody with highly efficient infected cell killing capacity for novel immunotherapeutic approaches. Emerg Microbes Infect. 2025;14:2432345 pubmed publisher |
| Murali T, Gu Y, Minhat R, Yap J, Wood K, WANG C, et al. Antigen-antibody complex density and antibody-induced HLA protein unfolding influence Fc-mediated antibody effector function. Front Immunol. 2024;15:1438285 pubmed publisher |
| Meira M, Frey A, Chekkat N, Rybczynska M, Sellam Z, Park J, et al. Targeting RGMb interactions: Discovery and preclinical characterization of potent anti-RGMb antibodies blocking multiple ligand bindings. MAbs. 2024;16:2432403 pubmed publisher |
| Martyn G, Kalagiri R, Veggiani G, Stanfield R, Choudhuri I, Sala M, et al. Using phage display for rational engineering of a higher affinity humanized 3' phosphohistidine-specific antibody. bioRxiv. 2024;: pubmed publisher |
| Ferrara F, Fanni A, Teixeira A, Molina E, Leal Lopes C, DeAguero A, et al. A next-generation Fab library platform directly yielding drug-like antibodies with high affinity, diversity, and developability. MAbs. 2024;16:2394230 pubmed publisher |
| Zhang L, Zheng B, Lu J, Wu H, Wu H, Zhang Q, et al. Evaluation of human antibodies from vaccinated volunteers for protection against Yersinia pestis infection. Microbiol Spectr. 2024;12:e0105424 pubmed publisher |
| Yang H, Chae J, Kim H, Noh J, Chung J. Selection of Antigen Binders from a Chicken Single-Chain Variable Fragment Library. Cold Spring Harb Protoc. 2024;: pubmed publisher |
| Zhu L, Huang B, Wang X, Ni F, Ao M, Wang R, et al. Highly potent and broadly neutralizing anti-CD4 trimeric nanobodies inhibit HIV-1 infection by inducing CD4 conformational alteration. Nat Commun. 2024;15:6961 pubmed publisher |
| Xiang S, Zhu C, Zhou Y, Wu W, Zhang Y, Chen C, et al. Facile Generation of Neutralizing Antibodies on Tyrosine Phosphorylated IRS1 by Epitope-Directed Elicitation. ACS Chem Biol. 2024;19:2050-2059 pubmed publisher |
| Ahmad M, Amorim C, Abu Qatouseh L, Montenegro M. Nanobody-based immunosensor for the detection of H. pylori in saliva. Biosens Bioelectron. 2024;260:116423 pubmed publisher |
| Lin K, Xia B, Wang X, He X, Zhou M, Lin Y, et al. Development of nanobodies targeting hepatocellular carcinoma and application of nanobody-based CAR-T technology. J Transl Med. 2024;22:349 pubmed publisher |
| Chen L, Sun M, Zhang H, Zhang X, Yao Y, Li M, et al. Potent human neutralizing antibodies against Nipah virus derived from two ancestral antibody heavy chains. Nat Commun. 2024;15:2987 pubmed publisher |
| Roodink I, van Erp M, Li A, Potter S, van Duijnhoven S, Smits M, et al. Broad Epitope Coverage of Therapeutic Multi-Antibody Combinations Targeting SARS-CoV-2 Boosts In Vivo Protection and Neutralization Potency to Corner an Immune-Evading Virus. Biomedicines. 2024;12: pubmed publisher |
| Rivera de Torre E, Lampadariou S, M xf8 iniche M, Bohn M, Kazemi S, Laustsen A. Discovery of broadly-neutralizing antibodies against brown recluse spider and Gadim scorpion sphingomyelinases using consensus toxins as antigens. Protein Sci. 2024;33:e4901 pubmed publisher |
| Smyth P, Ferguson L, Burrows J, Burden R, Tracey S, Herron x, et al. Evaluation of variable new antigen receptors (vNARs) as a novel cathepsin S (CTSS) targeting strategy. Front Pharmacol. 2023;14:1296567 pubmed publisher |
| Ganji M, Safarzadeh Kozani P, Rahbarizadeh F. Characterization of novel CD19-specific VHHs isolated from a camelid immune library by phage display. J Transl Med. 2023;21:891 pubmed publisher |
| Li D, Wang R, Liang T, Ren H, Park C, Tai C, et al. Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumours. Nat Commun. 2023;14:5920 pubmed publisher |
| Amir A, Taussig D, Bitton A, Nahary L, Vaisman Mentesh A, Benhar I, et al. Antibody Isolation from Human Synthetic Libraries of Single-Chain Antibodies and Analysis Using NGS. Methods Mol Biol. 2023;2702:347-372 pubmed publisher |
| Grahn A, Allen G, Kay B. Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly. Methods Mol Biol. 2023;2702:191-203 pubmed publisher |
| Rossotti M, Trempe F, van Faassen H, Hussack G, Arbabi Ghahroudi M. Isolation and Characterization of Single-Domain Antibodies from Immune Phage Display Libraries. Methods Mol Biol. 2023;2702:107-147 pubmed publisher |
| Liu C, Yang Q, Lin H, Cao L, Wang K, Sui J. Biopanning, Heterologous Expression, and Characterization of a Shark-Derived Single-Domain Antibody Fusion Protein against Pancreatic Lipase. ACS Biomater Sci Eng. 2023;9:3219-3226 pubmed publisher |
| Bai M, Wang Y, Zhang C, Wang Y, Wei J, Liao X, et al. Nanobody-based immunomagnetic separation platform for rapid isolation and detection of Salmonella enteritidis in food samples. Food Chem. 2023;424:136416 pubmed publisher |
| Kliche J, Garvanska D, Simonetti L, Badgujar D, Dobritzsch D, Nilsson J, et al. Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions. Mol Syst Biol. 2023;19:e11164 pubmed publisher |
| Yang H, Kim M, Raju C, Cho C, Park T, Park J. Highly sensitive and label-free electrochemical detection of C-reactive protein on a peptide receptor-gold nanoparticle-black phosphorous nanocomposite modified electrode. Biosens Bioelectron. 2023;234:115382 pubmed publisher |
| Li W, Wang F, Li Y, Yan L, Liu L, Zhu W, et al. Potent NTD-Targeting Neutralizing Antibodies against SARS-CoV-2 Selected from a Synthetic Immune System. Vaccines (Basel). 2023;11: pubmed publisher |
| Jiang X, Sun S, Li Z, Chen M. Isolation and Characterization of Targeting-HBsAg VNAR Single Domain Antibodies from Whitespotted Bamboo Sharks (Chiloscyllium plagiosum). Mar Drugs. 2023;21: pubmed publisher |
| Wang X, Zhu X, Wang D, Li X, Wang J, Yin G, et al. Identification of a Specific Phage as Growth Factor Alternative Promoting the Recruitment and Differentiation of MSCs in Bone Tissue Regeneration. ACS Biomater Sci Eng. 2023;9:2426-2437 pubmed publisher |
| Kim J, Shin J, Park B, Cho C, Huh Y, Choi C, et al. Harnessing protein sensing ability of electrochemical biosensors via a controlled peptide receptor-electrode interface. J Nanobiotechnology. 2023;21:100 pubmed publisher |
| Qin Y, Wu G, Jin J, Wang H, Zhang J, Liu L, et al. A fully human connective tissue growth factor blocking monoclonal antibody ameliorates experimental rheumatoid arthritis through inhibiting angiogenesis. BMC Biotechnol. 2023;23:6 pubmed publisher |
| Altendorf T, Gering I, Santiago Sch xfc bel B, Aghabashlou Saisan S, Tamg xfc ney G, Tusche M, et al. Stabilization of Monomeric Tau Protein by All D-Enantiomeric Peptide Ligands as Therapeutic Strategy for Alzheimer's Disease and Other Tauopathies. Int J Mol Sci. 2023;24: pubmed publisher |
| Zhao J, Sun Y, Yuan C, Li T, Liang Y, Zou H, et al. Quercetin ameliorates hepatic fat accumulation in high-fat diet-induced obese mice via PPARs. Food Funct. 2023;14:1674-1684 pubmed publisher |
| Jaiswal D, Kumar U, Gaur V, Salunke D. Epitope-directed anti-SARS-CoV-2 scFv engineered against the key spike protein region could block membrane fusion. Protein Sci. 2023;32:e4575 pubmed publisher |
| Hjelm L, Lindberg H, St xe5 hl S, L xf6 fblom J. Construction and Validation of a New Naïve Sequestrin Library for Directed Evolution of Binders against Aggregation-Prone Peptides. Int J Mol Sci. 2023;24: pubmed publisher |
| Yonehara R, Kumachi S, Kashiwagi K, Wakabayashi Nakao K, Motohashi M, Murakami T, et al. A novel agonist with homobivalent single-domain antibodies that bind the FGF receptor 1 domain III functions as an FGF2 ligand. J Biol Chem. 2023;299:102804 pubmed publisher |
| Ge Q, Teng M, Li X, Guo Q, Tao Y. An epitope-directed selection strategy facilitating the identification of Frizzled receptor selective antibodies. Structure. 2023;31:33-43.e5 pubmed publisher |
| Wang P, Yu G, Wei J, Liao X, Zhang Y, Ren Y, et al. A single thiolated-phage displayed nanobody-based biosensor for label-free detection of foodborne pathogen. J Hazard Mater. 2023;443:130157 pubmed publisher |
| Zhang T, Liu C, Zheng H, Han X, Lin H, Cao L, et al. The specific biopanning of single-domain antibody against haptens based on a functionalized cryogel. J Mol Recognit. 2023;36:e2999 pubmed publisher |
| Ma J, Xu X, Fu C, Xia P, Tian M, Zheng L, et al. CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin. Biomater Res. 2022;26:64 pubmed publisher |
| Jeong S, Zhang H, Yamaki S, Yang C, McKemy D, Lieber M, et al. Immunoglobulin somatic hypermutation in a defined biochemical system recapitulates affinity maturation and permits antibody optimization. Nucleic Acids Res. 2022;50:11738-11754 pubmed publisher |
| Zhao D, Liu L, Liu X, Zhang J, Yin Y, Luan L, et al. A potent synthetic nanobody with broad-spectrum activity neutralizes SARS-CoV-2 virus and the Omicron variant BA.1 through a unique binding mode. J Nanobiotechnology. 2022;20:411 pubmed publisher |
| Seo H, Lubis A, Lee S. A Novel Specific Single-Chain Variable Fragment Diagnostic System for Viral Hemorrhagic Septicemia Virus. Mar Biotechnol (NY). 2022;24:979-990 pubmed publisher |
| HOANG P, Luong Q, Cho S, Lee Y, Na K, Ayun R, et al. Enhancing neutralizing activity against influenza H1N1/PR8 by engineering a single-domain VL-M2 specific into a bivalent form. PLoS ONE. 2022;17:e0273934 pubmed publisher |
| Liu J, Ma P, Yu H, Wang M, Yin P, Pang S, et al. Discovery of a Phage Peptide Specifically Binding to the SARS-CoV-2 Spike S1 Protein for the Sensitive Phage-Based Enzyme-Linked Chemiluminescence Immunoassay of the SARS-CoV-2 Antigen. Anal Chem. 2022;94:11591-11599 pubmed publisher |
| Hu X, Zhang X, Liu Y, Gao M, Lin M, Xie Y, et al. Generation of Human Domain Antibody Fragments as Potential Insecticidal Agents against Helicoverpa armigera by Cadherin-Based Screening. J Agric Food Chem. 2022;70:11510-11519 pubmed publisher |
| Kim J, Shin J, Cho C, Hwang J, Kweon D, Park T, et al. Dual synergistic response for the electrochemical detection of H1N1 virus and viral proteins using high affinity peptide receptors. Talanta. 2022;248:123613 pubmed publisher |
| Zhao L, Chen M, Wang X, Kang S, Xue W, Li Z. Identification of Anti-TNFα VNAR Single Domain Antibodies from Whitespotted Bambooshark (Chiloscyllium plagiosum). Mar Drugs. 2022;20: pubmed publisher |
| Zhao H, Ren J, Wu S, Guo H, Du Y, Wan B, et al. HRP-conjugated-nanobody-based cELISA for rapid and sensitive clinical detection of ASFV antibodies. Appl Microbiol Biotechnol. 2022;106:4269-4285 pubmed publisher |
| Wang Z, Hu N, Zhou Y, Shi N, Shen B, Luo L, et al. Structure-guided affinity maturation of a novel human antibody targeting the SARS-CoV-2 nucleocapsid protein. Sci Rep. 2022;12:8469 pubmed publisher |
| Hu C, Chang Y, Liu C, Yu Y, Mou K. Development of a TNF-α-mediated Trojan Horse for bacteria-based cancer therapy. Mol Ther. 2022;30:2522-2536 pubmed publisher |
| Zhu Q, Hu X, Liu Y, Xie Y, Xu C, Lin M, et al. Identification of single domain antibodies with insect cytotoxicity using phage-display antibody library screening and Plutella xylostella ATP-binding cassette transporter subfamily C member 2 (ABCC2) -based insect cell expression system. Int J Biol Macromol. 2022;209:586-596 pubmed publisher |
| Shin J, Gul A, Hyun M, Choi C, Park T, Park J. Electrochemical detection of caspase-3 based on a chemically modified M13 phage virus. Bioelectrochemistry. 2022;145:108090 pubmed publisher |
| Laughlin T, Horn J. Engineering pH-Sensitive Single-Domain Antibodies. Methods Mol Biol. 2022;2446:269-298 pubmed publisher |
| Ge S, Wu R, Zhou T, Liu X, Zhu J, Zhang X. Specific anti-SARS-CoV-2 S1 IgY-scFv is a promising tool for recognition of the virus. AMB Express. 2022;12:18 pubmed publisher |
| Benz C, Ali M, Krystkowiak I, Simonetti L, Sayadi A, Mihalic F, et al. Proteome-scale mapping of binding sites in the unstructured regions of the human proteome. Mol Syst Biol. 2022;18:e10584 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Yuan T, Garg P, Wang L, Willis J, Kwan E, Hernandez A, et al. Rapid discovery of diverse neutralizing SARS-CoV-2 antibodies from large-scale synthetic phage libraries. MAbs. 2022;14:2002236 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Gransagne M, Aymé G, Brier S, Chauveau Le Friec G, Mériaux V, Nowakowski M, et al. Development of a highly specific and sensitive VHH-based sandwich immunoassay for the detection of the SARS-CoV-2 nucleoprotein. J Biol Chem. 2022;298:101290 pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Tan T, Patton E, Munro C, Corzo León D, Porter A, Palliyil S. Monoclonal Human Antibodies That Recognise the Exposed N and C Terminal Regions of the Often-Overlooked SARS-CoV-2 ORF3a Transmembrane Protein. Viruses. 2021;13: pubmed publisher |
| Qiang M, Ma P, Li Y, Liu H, Harding A, Min C, et al. Neutralizing Antibodies to SARS-CoV-2 Selected from a Human Antibody Library Constructed Decades Ago. Adv Sci (Weinh). 2021;:e2102181 pubmed publisher |
| Sulong P, Anudit N, Nuanualsuwan S, Mariela S, Khantasup K. Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2. PLoS ONE. 2021;16:e0258931 pubmed publisher |
| Tang J, Veggiani G, Singer A, Teyra J, Chung J, Sidhu S. A Panel of Engineered Ubiquitin Variants Targeting the Family of Domains Found in Ubiquitin Specific Proteases (DUSPs). J Mol Biol. 2021;433:167300 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Wu X, Cheng L, Fu M, Huang B, Zhu L, Xu S, et al. A potent bispecific nanobody protects hACE2 mice against SARS-CoV-2 infection via intranasal administration. Cell Rep. 2021;37:109869 pubmed publisher |
| Wang R, Zhang H, Peng C, Shi J, Zhang H, Gong R. Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus. Virol Sin. 2021;36:1600-1610 pubmed publisher |
| Zhao S, Zhang H, Yang X, Zhang H, Chen Y, Zhan Y, et al. Identification of potent human neutralizing antibodies against SARS-CoV-2 implications for development of therapeutics and prophylactics. Nat Commun. 2021;12:4887 pubmed publisher |
| Wang H, Chang Y, Hu C, Kao C, Yu Y, Lim S, et al. Development of a Novel Cytokine Vehicle Using Filamentous Phage Display for Colorectal Cancer Treatment. ACS Synth Biol. 2021;10:2087-2095 pubmed publisher |
| Santur K, Reinartz E, Lien Y, Tusche M, Altendorf T, Sevenich M, et al. Ligand-Induced Stabilization of the Native Human Superoxide Dismutase 1. ACS Chem Neurosci. 2021;12:2520-2528 pubmed publisher |
| Zhou J, Li Y, Liu Z, Qian W, Chen Y, Qi Y, et al. Induction of anti-Zearalenone immune response with mimotopes identified from a phage display peptide library. Toxicon. 2021;199:1-6 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Noy Porat T, Mechaly A, Levy Y, Makdasi E, Alcalay R, Gur D, et al. Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice. iScience. 2021;24:102479 pubmed publisher |
| Zhao S, Li C, Gao Y, Qian L, Dong J, Zhai L, et al. Screening and Identification of a Specific Binding Peptide to Ovarian Cancer Cells from a Phage-Displayed Peptide Library. Int J Pept Res Ther. 2021;27:1741-1749 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Xu M, Lei G, Chen M, Wang K, Lv W, Zhang P, et al. Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy. EBioMedicine. 2021;65:103250 pubmed publisher |
| Chang Y, Kao C, Tang H, Huang M, Mou K. Direct Antibody Isolation on Cells Using Affinity-Tag-Guided Proximity Selection. Biochemistry. 2020;59:4285-4293 pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Du P, Wang X, Yin T, Zhang X, Zhang Z, Yu W, et al. Anti-tumor effect of single-chain antibody to Reg3a in colorectal cancer. Exp Cell Res. 2020;396:112278 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Noy Porat T, Makdasi E, Alcalay R, Mechaly A, Levy Y, Bercovich Kinori A, et al. A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes. Nat Commun. 2020;11:4303 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Wu X, Li Y, Huang B, Ma X, Zhu L, Zheng N, et al. A single-domain antibody inhibits SFTSV and mitigates virus-induced pathogenesis in vivo. JCI Insight. 2020;5: pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Cho S, Kil E, Cho S, Byun H, Kang E, Choi H, et al. Development of novel detection system for sweet potato leaf curl virus using recombinant scFv. Sci Rep. 2020;10:8039 pubmed publisher |
- ELISA; unidentified
- ELISA; mouse
| Feng R, Wang R, Hong J, Dower C, Croix B, Ho M. Isolation of rabbit single domain antibodies to B7-H3 via protein immunization and phage display. Antib Ther. 2020;3:10-17 pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Dong S, Gao M, Bo Z, Guan L, Hu X, Zhang H, et al. Production and characterization of a single-chain variable fragment antibody from a site-saturation mutagenesis library derived from the anti-Cry1A monoclonal antibody. Int J Biol Macromol. 2020;149:60-69 pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Kim Y, Lee H, Park K, Park S, Lim J, So M, et al. Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning. Antibodies (Basel). 2019;8: pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Tong Y, Zhong S, Shan Z, Yao W, Tian H. A novel human anti-BAFF neutralizing monoclonal antibody derived from in vitro immunization. Biomed Pharmacother. 2019;119:109430 pubmed publisher |
- ELISA; mouse
- ELISA; unidentified
| Xing L, Xu Y, Sun K, Wang H, Zhang F, Zhou Z, et al. Identification of a peptide for folate receptor alpha by phage display and its tumor targeting activity in ovary cancer xenograft. Sci Rep. 2018;8:8426 pubmed publisher |
| Benz C, Maassen L, Simonetti L, Mihalic F, Lindqvist R, Tsitsa I, et al. Defining short linear motif binding determinants by phage display-based deep mutational scanning. Protein Sci. 2025;34:e70174 pubmed publisher |
| Wei Z, Yuan J, Chen Q, Pang J, Shi Q, Peng B, et al. Bifunctional chemokine-nanobody fusion protein enhances neutrophil recruitment to impede Acanthamoeba immune evasion. EBioMedicine. 2025;115:105685 pubmed publisher |
