| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot knockout validation; mouse; 1:10,000; fig s3a
| Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher |
- western blot; mouse; fig 2i
| Silva C, Peyre E, Adhikari M, Tielens S, Tanco S, Van Damme P, et al. Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell. 2018;172:1063-1078.e19 pubmed publisher |
- western blot; human; fig 1
| Kim D, Helfman D. Loss of MLCK leads to disruption of cell-cell adhesion and invasive behavior of breast epithelial cells via increased expression of EGFR and ERK/JNK signaling. Oncogene. 2016;35:4495-508 pubmed publisher |
- western blot; human; 1:1000
| Contreras T, Ricciardi E, Cremonini E, Oteiza P. (-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity. Arch Biochem Biophys. 2015;573:84-91 pubmed publisher |
| Wang Y, Zhao W, Zhang L, Zhao Y, Li F, Zhang Z, et al. Molecular and cellular basis of the regulation of lymphatic contractility and lymphatic absorption. Int J Biochem Cell Biol. 2014;53:134-40 pubmed publisher |
- western blot; rat; 1:5000
| Liu F, Wang X, Hu G, Wang Y, Zhou J. The transcription factor TEAD1 represses smooth muscle-specific gene expression by abolishing myocardin function. J Biol Chem. 2014;289:3308-16 pubmed publisher |
- immunocytochemistry; mouse
- western blot; mouse
| Xu J, Gao X, Ramchandran R, Zhao Y, Vogel S, Malik A. Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins. Nat Immunol. 2008;9:880-6 pubmed publisher |
| Weinberger M, Sim es F, Patient R, Sauka Spengler T, Riley P. Functional Heterogeneity within the Developing Zebrafish Epicardium. Dev Cell. 2020;: pubmed publisher |
| Schaub T, Gürgen D, Maus D, Lange C, Tarabykin V, Dragun D, et al. mTORC1 and mTORC2 Differentially Regulate Cell Fate Programs to Coordinate Osteoblastic Differentiation in Mesenchymal Stromal Cells. Sci Rep. 2019;9:20071 pubmed publisher |
| Wu Y, Zhen W, Geng Y, Wang Z, Guo Y. Pretreatment with probiotic Enterococcus faecium NCIMB 11181 ameliorates necrotic enteritis-induced intestinal barrier injury in broiler chickens. Sci Rep. 2019;9:10256 pubmed publisher |
| Graham W, He W, Marchiando A, Zha J, Singh G, Li H, et al. Intracellular MLCK1 diversion reverses barrier loss to restore mucosal homeostasis. Nat Med. 2019;25:690-700 pubmed publisher |
| Wang G, Sun G, Wang Y, Yu P, Wang X, Zhou B, et al. Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p-MLC signaling pathway. Exp Ther Med. 2019;17:107-114 pubmed publisher |
| Sarker M, Aki S, Yoshioka K, Kuno K, Okamoto Y, Ishimaru K, et al. Class II PI3Ks α and β Are Required for Rho-Dependent Uterine Smooth Muscle Contraction and Parturition in Mice. Endocrinology. 2019;160:235-248 pubmed publisher |
| Christie M, Romano T, Murphy R, Posterino G. The effect of intrauterine growth restriction on Ca2+ -activated force and contractile protein expression in the mesenteric artery of adult (6-month-old) male and female Wistar-Kyoto rats. Physiol Rep. 2018;6:e13954 pubmed publisher |
| Luh C, Feiler S, Frauenknecht K, Meyer S, Lubomirov L, Neulen A, et al. The Contractile Apparatus Is Essential for the Integrity of the Blood-Brain Barrier After Experimental Subarachnoid Hemorrhage. Transl Stroke Res. 2018;: pubmed publisher |
| Sabui S, Kapadia R, Ghosal A, Schneider M, Lambrecht N, Said H. Biotin and pantothenic acid oversupplementation to conditional SLC5A6 KO mice prevents the development of intestinal mucosal abnormalities and growth defects. Am J Physiol Cell Physiol. 2018;315:C73-C79 pubmed publisher |
| Guo L, Cai T, Chen K, Wang R, Wang J, Cui C, et al. Kindlin-2 regulates mesenchymal stem cell differentiation through control of YAP1/TAZ. J Cell Biol. 2018;217:1431-1451 pubmed publisher |
| Chopyk D, Kumar P, Raeman R, Liu Y, Smith T, Anania F. Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers. Physiol Rep. 2017;5: pubmed publisher |
| Nighot M, Al Sadi R, Guo S, Rawat M, Nighot P, Watterson M, et al. Lipopolysaccharide-Induced Increase in Intestinal Epithelial Tight Permeability Is Mediated by Toll-Like Receptor 4/Myeloid Differentiation Primary Response 88 (MyD88) Activation of Myosin Light Chain Kinase Expression. Am J Pathol. 2017;187:2698-2710 pubmed publisher |
| Kassianidou E, Hughes J, Kumar S. Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation. Mol Biol Cell. 2017;28:3832-3843 pubmed publisher |
| Wu R, Vazquez Roque M, Carlson P, Burton D, Grover M, Camilleri M, et al. Gluten-induced symptoms in diarrhea-predominant irritable bowel syndrome are associated with increased myosin light chain kinase activity and claudin-15 expression. Lab Invest. 2017;97:14-23 pubmed publisher |
| Sabui S, Bohl J, Kapadia R, Cogburn K, Ghosal A, Lambrecht N, et al. Role of the sodium-dependent multivitamin transporter (SMVT) in the maintenance of intestinal mucosal integrity. Am J Physiol Gastrointest Liver Physiol. 2016;311:G561-70 pubmed publisher |
| Xu F, Ahmed A, Kang X, Hu G, Liu F, Zhang W, et al. MicroRNA-15b/16 Attenuates Vascular Neointima Formation by Promoting the Contractile Phenotype of Vascular Smooth Muscle Through Targeting YAP. Arterioscler Thromb Vasc Biol. 2015;35:2145-52 pubmed publisher |
| Cheng X, Wang X, Wan Y, Zhou Q, Zhu H, Wang Y. Myosin light chain kinase inhibitor ML7 improves vascular endothelial dysfunction via tight junction regulation in a rabbit model of atherosclerosis. Mol Med Rep. 2015;12:4109-4116 pubmed publisher |
| Reho J, Zheng X, Asico L, Fisher S. Redox signaling and splicing dependent change in myosin phosphatase underlie early versus late changes in NO vasodilator reserve in a mouse LPS model of sepsis. Am J Physiol Heart Circ Physiol. 2015;308:H1039-50 pubmed publisher |
| Li Y, Sun M, Zhao Y, Fu X, Xu H, Liu J. Oridonin suppress cell migration via regulation of nonmuscle myosin IIA. Cytotechnology. 2016;68:389-97 pubmed publisher |
| Chen C, Tao T, Wen C, He W, Qiao Y, Gao Y, et al. Myosin light chain kinase (MLCK) regulates cell migration in a myosin regulatory light chain phosphorylation-independent mechanism. J Biol Chem. 2014;289:28478-88 pubmed publisher |
| Yu Z, Tan J, McMahon A, Iismaa S, Xiao X, Kesteven S, et al. RhoA/ROCK signaling and pleiotropic ?1A-adrenergic receptor regulation of cardiac contractility. PLoS ONE. 2014;9:e99024 pubmed publisher |
| Tsai M, Chang A, Huang J, He W, Sweeney H, Zhu M, et al. Constitutive phosphorylation of myosin phosphatase targeting subunit-1 in smooth muscle. J Physiol. 2014;592:3031-51 pubmed publisher |
| Hadeed J, Bond J, Selim M, Bergeron A, Levin L, Levinson H. Calcium-dependent signaling in Dupuytren's disease. Hand (N Y). 2011;6:159-64 pubmed publisher |
| Bhuwania R, Cornfine S, Fang Z, Kruger M, Luna E, Linder S. Supervillin couples myosin-dependent contractility to podosomes and enables their turnover. J Cell Sci. 2012;125:2300-14 pubmed publisher |
| Miao H, Crabb A, Hernandez M, Lukas T. Modulation of factors affecting optic nerve head astrocyte migration. Invest Ophthalmol Vis Sci. 2010;51:4096-103 pubmed publisher |
| Wang G, Yeh C, Jensen B, Mann M, Simpson P, Baker A. Heart failure switches the RV alpha1-adrenergic inotropic response from negative to positive. Am J Physiol Heart Circ Physiol. 2010;298:H913-20 pubmed publisher |
| Leguillette R, Laviolette M, Bergeron C, Zitouni N, Kogut P, Solway J, et al. Myosin, transgelin, and myosin light chain kinase: expression and function in asthma. Am J Respir Crit Care Med. 2009;179:194-204 pubmed publisher |
| Injeti E, Sandoval R, Williams J, Smolensky A, Ford L, Pearce W. Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries. Am J Physiol Heart Circ Physiol. 2008;295:H2289-98 pubmed publisher |
| Lukas T, Miao H, Chen L, Riordan S, Li W, Crabb A, et al. Susceptibility to glaucoma: differential comparison of the astrocyte transcriptome from glaucomatous African American and Caucasian American donors. Genome Biol. 2008;9:R111 pubmed publisher |
| Fairbank N, Connolly S, Mackinnon J, Wehry K, Deng L, Maksym G. Airway smooth muscle cell tone amplifies contractile function in the presence of chronic cyclic strain. Am J Physiol Lung Cell Mol Physiol. 2008;295:L479-88 pubmed publisher |
| Pucovský V, Harhun M, Povstyan O, Gordienko D, Moss R, Bolton T. Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell. J Cell Mol Med. 2007;11:764-75 pubmed |
| Sandoval R, Injeti E, Gerthoffer W, Pearce W. Postnatal maturation modulates relationships among cytosolic Ca2+, myosin light chain phosphorylation, and contractile tone in ovine cerebral arteries. Am J Physiol Heart Circ Physiol. 2007;293:H2183-92 pubmed |
| Zhang M, Fang H, Zhou J, Herring B. A novel role of Brg1 in the regulation of SRF/MRTFA-dependent smooth muscle-specific gene expression. J Biol Chem. 2007;282:25708-16 pubmed |
| Zhang H, Fisher S. Conditioning effect of blood flow on resistance artery smooth muscle myosin phosphatase. Circ Res. 2007;100:730-7 pubmed |
| Wyckoff J, Pinner S, Gschmeissner S, Condeelis J, Sahai E. ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo. Curr Biol. 2006;16:1515-23 pubmed |
| Taylor J, Zhu Q, Irwin B, Maghaydah Y, Tsimikas J, Pilbeam C, et al. Null mutation in macrophage migration inhibitory factor prevents muscle cell loss and fibrosis in partial bladder outlet obstruction. Am J Physiol Renal Physiol. 2006;291:F1343-53 pubmed |
| Yin F, Hoggatt A, Zhou J, Herring B. 130-kDa smooth muscle myosin light chain kinase is transcribed from a CArG-dependent, internal promoter within the mouse mylk gene. Am J Physiol Cell Physiol. 2006;290:C1599-609 pubmed |
| Goffin J, Pittet P, Csucs G, Lussi J, Meister J, Hinz B. Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers. J Cell Biol. 2006;172:259-68 pubmed |
| Goeckeler Z, Wysolmerski R. Myosin phosphatase and cofilin mediate cAMP/cAMP-dependent protein kinase-induced decline in endothelial cell isometric tension and myosin II regulatory light chain phosphorylation. J Biol Chem. 2005;280:33083-95 pubmed |
| Wang F, Graham W, Wang Y, Witkowski E, Schwarz B, Turner J. Interferon-gamma and tumor necrosis factor-alpha synergize to induce intestinal epithelial barrier dysfunction by up-regulating myosin light chain kinase expression. Am J Pathol. 2005;166:409-19 pubmed |
| Emmert D, Fee J, Goeckeler Z, Grojean J, Wakatsuki T, Elson E, et al. Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts. Am J Physiol Cell Physiol. 2004;286:C8-21 pubmed |
| Moir L, Leung S, Eynott P, McVicker C, Ward J, Chung K, et al. Repeated allergen inhalation induces phenotypic modulation of smooth muscle in bronchioles of sensitized rats. Am J Physiol Lung Cell Mol Physiol. 2003;284:L148-59 pubmed |
| Jindo T, Wine R, Li L, Chapin R. Protein kinase activity is central to rat germ cell apoptosis induced by methoxyacetic acid. Toxicol Pathol. 2001;29:607-16 pubmed |
| Gao Y, Ye L, Kishi H, Okagaki T, Samizo K, Nakamura A, et al. Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction. IUBMB Life. 2001;51:337-44 pubmed |
| Poperechnaya A, Varlamova O, Lin P, Stull J, Bresnick A. Localization and activity of myosin light chain kinase isoforms during the cell cycle. J Cell Biol. 2000;151:697-708 pubmed |
| Nguyen D, Catling A, Webb D, Sankovic M, Walker L, Somlyo A, et al. Myosin light chain kinase functions downstream of Ras/ERK to promote migration of urokinase-type plasminogen activator-stimulated cells in an integrin-selective manner. J Cell Biol. 1999;146:149-64 pubmed |
| Topouzis S, Majesky M. Smooth muscle lineage diversity in the chick embryo. Two types of aortic smooth muscle cell differ in growth and receptor-mediated transcriptional responses to transforming growth factor-beta. Dev Biol. 1996;178:430-45 pubmed |
