Anti-EB1 antibody produced in rabbit | MilliporeSigma E3406 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

antibody

product name :

Anti-EB1 antibody produced in rabbit

catalog :

E3406

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3c	Thomas G, Bandopadhyay K, Sutradhar S, Renjith M, Singh P, Gireesh K, et al. EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice. Nat Commun. 2016;7:11665 pubmed publisher
	Fang C, Kuo H, Hsu S, Yih L. HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes. Cell Div. 2019;14:4 pubmed publisher

product information

cat no :

E3406

brand :

SIGMA

name :

Anti-EB1 antibody produced in rabbit

prod type :

Chemical

name suffix :

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

storage :

-20C

storage temp :

-20C

physical form :

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

shipped in :

dry ice

syns :

Anti-APC binding protein EB1; Anti-End-Binding-1; Anti-Microtuble-associated protein RP/EB family member 1

mdl no :

MFCD01633308

unspsc code :

12352203

wgk :

form :

buffered aqueous solution

antibody form :

affinity isolated antibody

application(s) :

western blot: 0.5-1 mug/mL using cell lysate of the rat fibroblast cell line Rat1; indirect immunofluorescence: 1-2 mug/mL using rat fibroblast Rat1 cells; immunoprecipitation: 10-20 mug using cell lysate of the human epitheloid carcinoma HeLa cell line; indirect immunofluorescence: suitable

species reactivity :

rat, human, bovine, pig, canine, mouse

concentration :

~1.5 mg/mL

immunogen :

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

features and benefits :

Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

mol wt :

antigen mol wt 35 kDa