product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Monoclonal Anti-Dystrophin antibody produced in mouse
catalog :
D8168
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MANDYS8
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
citations: 45
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; 1:1000; fig 5D
Ousterout D, Kabadi A, Thakore P, Majoros W, Reddy T, Gersbach C. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy. Nat Commun. 2015;6:6244 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2
  • western blot; mouse; loading ...; fig 3a
Zhang Y, Li H, Min Y, Sanchez Ortiz E, Huang J, Mireault A, et al. Enhanced CRISPR-Cas9 correction of Duchenne muscular dystrophy in mice by a self-complementary AAV delivery system. Sci Adv. 2020;6:eaay6812 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Min Y, Li H, Rodriguez Caycedo C, Mireault A, Huang J, Shelton J, et al. CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells. Sci Adv. 2019;5:eaav4324 pubmed publisher
  • immunohistochemistry; human; 1:800; fig 3a
  • western blot; human; fig 3d
Long C, Li H, Tiburcy M, Rodriguez Caycedo C, Kyrychenko V, Zhou H, et al. Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing. Sci Adv. 2018;4:eaap9004 pubmed publisher
  • immunohistochemistry; mouse; 1:400; fig s8
Park S, Yun Y, Lim J, Kim M, Kim S, Kim J, et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun. 2016;7:10871 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:400; fig 3
Strauss J, Shaw C, Bradley H, Wilson O, Dorval T, Pilling J, et al. Immunofluorescence microscopy of SNAP23 in human skeletal muscle reveals colocalization with plasma membrane, lipid droplets, and mitochondria. Physiol Rep. 2016;4: pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig s20a
Nelson C, Hakim C, Ousterout D, Thakore P, Moreb E, Castellanos Rivera R, et al. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2016;351:403-7 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s4a, s13b
  • western blot; mouse; 1:1000; loading ...; fig s8a, s8c, s13c
Long C, Amoasii L, Mireault A, McAnally J, Li H, Sanchez Ortiz E, et al. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular dystrophy. Science. 2016;351:400-3 pubmed publisher
  • immunohistochemistry - frozen section; human
Molsted S, Andersen J, Harrison A, Eidemak I, Mackey A. Fiber type-specific response of skeletal muscle satellite cells to high-intensity resistance training in dialysis patients. Muscle Nerve. 2015;52:736-45 pubmed publisher
  • western blot; human; 1:1000
Ousterout D, Kabadi A, Thakore P, Perez Pinera P, Brown M, Majoros W, et al. Correction of dystrophin expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger nucleases. Mol Ther. 2015;23:523-32 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Gouspillou G, Sgarioto N, Norris B, Barbat Artigas S, Aubertin Leheudre M, Morais J, et al. The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans. PLoS ONE. 2014;9:e103044 pubmed publisher
Amoasii L, Li H, Zhang Y, Min Y, Sanchez Ortiz E, Shelton J, et al. In vivo non-invasive monitoring of dystrophin correction in a new Duchenne muscular dystrophy reporter mouse. Nat Commun. 2019;10:4537 pubmed publisher
Elia A, Wilson O, Lees M, Parker P, Barlow M, Cocks M, et al. Skeletal muscle, haematological and splenic volume characteristics of elite breath-hold divers. Eur J Appl Physiol. 2019;119:2499-2511 pubmed publisher
Soendenbroe C, Heisterberg M, Schjerling P, Karlsen A, Kjaer M, Andersen J, et al. Molecular indicators of denervation in aging human skeletal muscle. Muscle Nerve. 2019;60:453-463 pubmed publisher
Ito A, Ohnuki Y, Suita K, Ishikawa M, Mototani Y, Shiozawa K, et al. Role of β-adrenergic signaling in masseter muscle. PLoS ONE. 2019;14:e0215539 pubmed publisher
Nelson C, Wu Y, Gemberling M, Oliver M, Waller M, Bohning J, et al. Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy. Nat Med. 2019;25:427-432 pubmed publisher
Leduc Gaudet J, Reynaud O, Hussain S, Gouspillou G. Parkin overexpression protects from ageing-related loss of muscle mass and strength. J Physiol. 2019;597:1975-1991 pubmed publisher
Yuan J, Ma Y, Huang T, Chen Y, Peng Y, Li B, et al. Genetic Modulation of RNA Splicing with a CRISPR-Guided Cytidine Deaminase. Mol Cell. 2018;72:380-394.e7 pubmed publisher
Wang D, Mokhonova E, Kendall G, Becerra D, Naeini Y, Cantor R, et al. Repurposing Dantrolene for Long-Term Combination Therapy to Potentiate Antisense-Mediated DMD Exon Skipping in the mdx Mouse. Mol Ther Nucleic Acids. 2018;11:180-191 pubmed publisher
Morano M, Ronchi G, Nicolò V, Fornasari B, Crosio A, Perroteau I, et al. Modulation of the Neuregulin 1/ErbB system after skeletal muscle denervation and reinnervation. Sci Rep. 2018;8:5047 pubmed publisher
Ugwu F, Yu A, Sin T, Tam B, Lai C, Wong S, et al. Protective Effect of Unacylated Ghrelin on Compression-Induced Skeletal Muscle Injury Mediated by SIRT1-Signaling. Front Physiol. 2017;8:962 pubmed publisher
Mackey A, Kjaer M. The breaking and making of healthy adult human skeletal muscle in vivo. Skelet Muscle. 2017;7:24 pubmed publisher
Kyrychenko V, Kyrychenko S, Tiburcy M, Shelton J, Long C, Schneider J, et al. Functional correction of dystrophin actin binding domain mutations by genome editing. JCI Insight. 2017;2: pubmed publisher
Zhang Y, Long C, Li H, McAnally J, Baskin K, Shelton J, et al. CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice. Sci Adv. 2017;3:e1602814 pubmed publisher
Fuoco C, Rizzi R, Biondo A, Longa E, Mascaro A, Shapira Schweitzer K, et al. In vivo generation of a mature and functional artificial skeletal muscle. EMBO Mol Med. 2015;7:411-22 pubmed publisher
Chaudhuri O, Gu L, Darnell M, Klumpers D, Bencherif S, Weaver J, et al. Substrate stress relaxation regulates cell spreading. Nat Commun. 2015;6:6364 pubmed publisher
Kayman Kurekci G, Talim B, Korkusuz P, Sayar N, Sarioglu T, Oncel I, et al. Mutation in TOR1AIP1 encoding LAP1B in a form of muscular dystrophy: a novel gene related to nuclear envelopathies. Neuromuscul Disord. 2014;24:624-33 pubmed publisher
Buchholz B, Perez V, Siachoque N, Miksztowicz V, Berg G, Rodriguez M, et al. Dystrophin proteolysis: a potential target for MMP-2 and its prevention by ischemic preconditioning. Am J Physiol Heart Circ Physiol. 2014;307:H88-96 pubmed publisher
Gouspillou G, Sgarioto N, Kapchinsky S, Purves Smith F, Norris B, Pion C, et al. Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans. FASEB J. 2014;28:1621-33 pubmed publisher
Hollinger K, Yang C, Montz R, Nonneman D, Ross J, Selsby J. Dystrophin insufficiency causes selective muscle histopathology and loss of dystrophin-glycoprotein complex assembly in pig skeletal muscle. FASEB J. 2014;28:1600-9 pubmed publisher
Matsakas A, Yadav V, Lorca S, Narkar V. Muscle ERR? mitigates Duchenne muscular dystrophy via metabolic and angiogenic reprogramming. FASEB J. 2013;27:4004-16 pubmed publisher
Rougier J, Gavillet B, Abriel H. Proteasome inhibitor (MG132) rescues Nav1.5 protein content and the cardiac sodium current in dystrophin-deficient mdx (5cv) mice. Front Physiol. 2013;4:51 pubmed publisher
Rowan S, Rygiel K, Purves Smith F, Solbak N, Turnbull D, Hepple R. Denervation causes fiber atrophy and myosin heavy chain co-expression in senescent skeletal muscle. PLoS ONE. 2012;7:e29082 pubmed publisher
Teng B, Tam E, Benzie I, Siu P. Protective effect of caspase inhibition on compression-induced muscle damage. J Physiol. 2011;589:3349-69 pubmed publisher
Kielbasa O, Reynolds J, Wu C, Snyder C, Cho M, Weiler H, et al. Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration. FASEB J. 2011;25:2276-86 pubmed publisher
Thomas M, Khan W, Betik A, Wright K, Hepple R. Initiating exercise training in late middle age minimally protects muscle contractile function and increases myocyte oxidative damage in senescent rats. Exp Gerontol. 2010;45:856-67 pubmed publisher
Zanoteli E, van de Vlekkert D, Bonten E, Hu H, Mann L, Gomero E, et al. Muscle degeneration in neuraminidase 1-deficient mice results from infiltration of the muscle fibers by expanded connective tissue. Biochim Biophys Acta. 2010;1802:659-72 pubmed publisher
Siu P, Tam E, Teng B, Pei X, Ng J, Benzie I, et al. Muscle apoptosis is induced in pressure-induced deep tissue injury. J Appl Physiol (1985). 2009;107:1266-75 pubmed publisher
Oishi Y, Hayashida M, Tsukiashi S, Taniguchi K, Kami K, Roy R, et al. Heat stress increases myonuclear number and fiber size via satellite cell activation in rat regenerating soleus fibers. J Appl Physiol (1985). 2009;107:1612-21 pubmed publisher
Blitzblau R, Storer E, Jacob M. Dystrophin and utrophin isoforms are expressed in glia, but not neurons, of the avian parasympathetic ciliary ganglion. Brain Res. 2008;1218:21-34 pubmed publisher
Jansen K, Pavlath G. Mannose receptor regulates myoblast motility and muscle growth. J Cell Biol. 2006;174:403-13 pubmed
Jiang Y, Jahagirdar B, Reinhardt R, Schwartz R, Keene C, Ortiz Gonzalez X, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41-9 pubmed
Horsley V, Friday B, Matteson S, Kegley K, Gephart J, Pavlath G. Regulation of the growth of multinucleated muscle cells by an NFATC2-dependent pathway. J Cell Biol. 2001;153:329-38 pubmed
Sedgwick S, Nguyen T, Ellis J, Crowne H, Morris G. Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res. 1991;19:5889-94 pubmed
Nguyen thi Man -, Cartwright A, Morris G, Love D, Bloomfield J, Davies K. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett. 1990;262:237-40 pubmed
product information
Catalog Number :
D8168
Product Name :
Monoclonal Anti-Dystrophin antibody produced in mouse
Product Type :
ANTIBODIES - SIAL
Product Group :
Protein & Pathway Technologies
Host Species :
mouse
Clonality :
monoclonal
Conjugation :
unconjugated
Clone Name :
MANDYS8
Isotype :
IgG2b
Product Description :
clone MANDYS8, ascites fluid
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA