This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
chemical
product name :
U2OS Cells GFP-NUP98
catalog :
CLL1136
product information
name :
U2OS Cells GFP-NUP98
cat no :
CLL1136
brand :
SIGMA
prod type :
Chemical
storage temp :
-196C
storage :
-196C
legal information :
CompoZr is a registered trademark of Sigma-Aldrich Co. LLC; 3xFLAG is a trademark of Sigma-Aldrich Co. LLC
general description :
U2OS GFP-NUP98 are bone osteosarcoma cells from a human 15 year old caucasian female having a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus.This cell line was derived from ATCC Catalog No. HTB-96.
features and benefits :
Zinc finger nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag just behind the start codon of the NUP98 gene on chromosome 11p15.4 to create a cell line exhibiting stable expression of the transgene tagged with GFP on the N-terminus of the protein.The U2OS cells are adherent, with a doubling time of approx. 29 hours.
application :
This product is a human U2OS cell line in which the genomic NUP98 gene has been endogenously tagged with a 3XFLAG(TM) epitope and a Green Fluorescent Protein (GFP) gene using CompoZr(R) Zinc Finger Nuclease technology. Integration resulted in endogenous expression of a fusion protein in which the 3XFLAG is attached to the N-terminus of GFP and the GFP is attached to the N-terminus of NUP98. Fluorescence imaging shows characteristic NUP98 nuclear expression. This stable cell line was expanded from a single clone. The target's gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
company information
MilliporeSigma
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