product summary
company name :
MilliporeSigma
other brands :
FLUKA, Sigma-Aldrich, Roche Applied Science
product type :
antibody
product name :
Anti-γ-Actin antibody, Mouse monoclonal
catalog :
A8481
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2-2.1.14.17
reactivity :
human, mouse, chicken, bovine
application :
western blot, immunohistochemistry knockout validation
more info or order :
citations: 10
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; human; 1:2000; loading ...; fig 4b
Nagasaki A, Kato Y, Meguro K, Yamagishi A, Nakamura C, Uyeda T. A genome editing vector that enables easy selection and identification of knockout cells. Plasmid. 2018;98:37-44 pubmed publisher
  • western blot; human; loading ...; fig 3j
Zhang B, Chen H, Ouyang J, Xie Y, Chen L, Tan Q, et al. SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect. Autophagy. 2019;:1-17 pubmed publisher
  • western blot; human; loading ...; fig 2d
Ni Z, Kuang L, Chen H, Xie Y, Zhang B, Ouyang J, et al. The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis. Cell Death Dis. 2019;10:522 pubmed publisher
  • western blot; human; 1:1000; fig 4
Li J, Yang Z, Chen Z, Bao Y, Zhang H, Fang X, et al. ATF3 suppresses ESCC via downregulation of ID1. Oncol Lett. 2016;12:1642-1648 pubmed
Stackpole E, Akins M, Ivshina M, Murthy A, Fawzi N, Fallon J. EGFP insertional mutagenesis reveals multiple FXR2P fibrillar states with differing ribosome association in neurons. Biol Open. 2019;8: pubmed publisher
Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
Philimonenko V, Philimonenko A, Sloufová I, Hruby M, Novotny F, Halbhuber Z, et al. Simultaneous detection of multiple targets for ultrastructural immunocytochemistry. Histochem Cell Biol. 2014;141:229-39 pubmed publisher
Benz P, Merkel C, Offner K, Abeßer M, Ullrich M, Fischer T, et al. Mena/VASP and ?II-Spectrin complexes regulate cytoplasmic actin networks in cardiomyocytes and protect from conduction abnormalities and dilated cardiomyopathy. Cell Commun Signal. 2013;11:56 pubmed publisher
Gokhin D, Fowler V. Cytoplasmic gamma-actin and tropomodulin isoforms link to the sarcoplasmic reticulum in skeletal muscle fibers. J Cell Biol. 2011;194:105-20 pubmed publisher
Burrows C, Abd Latip N, Lam S, Carpenter L, Sawicka K, Tzolovsky G, et al. The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration. Nucleic Acids Res. 2010;38:5542-53 pubmed publisher
product information
Product No. :
A8481
Product Name :
Anti-γ-Actin antibody, Mouse monoclonal
Host :
mouse
Conjugate :
unconjugated
Antibody Type :
monoclonal
Clone Number (if monoclonal) :
2-2.1.14.17
Applications/Description :
Suitable for immunohistochemistry; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells; indirect ELISA
Species Reactivity :
bovine, human, canine, mouse, hamster, chicken
Isotype :
IgG1
Concentration :
~2 mg/mL
Storage :
Store at -20°C
more info or order :
company information
MilliporeSigma
PO Box 14508
St. Louis, MO 63178
https://www.sigmaaldrich.com
1-800-325-3010
headquarters: USA