Monoclonal Anti-alpha-Adaptin antibody produced in mouse | MilliporeSigma A4325 product information

product summary

company name :

MilliporeSigma

other brands :

FLUKA, Sigma-Aldrich, Roche Applied Science

product type :

antibody

product name :

Monoclonal Anti-alpha-Adaptin antibody produced in mouse

catalog :

A4325

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

100/2

The same clone is also sold as:

Boster: MA1105
Santa Cruz Biotechnology: sc-58215

reactivity :

human, mouse, rat, bovine

application :

western blot

more info or order :

MilliporeSigma product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 3a	Boonen M, Staudt C, Gilis F, Oorschot V, Klumperman J, Jadot M. Cathepsin D and its newly identified transport receptor SEZ6L2 can modulate neurite outgrowth. J Cell Sci. 2016;129:557-68 pubmed publisher
western blot; rat	Petralia R, Yao P. AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles. J Comp Neurol. 2007;504:314-27 pubmed
	Tower Gilchrist C, Zlatic S, Yu D, Chang Q, Wu H, Lin X, et al. Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear. Mol Biol Cell. 2019;:mbcE16080592 pubmed publisher
	Pascolutti R, Algisi V, Conte A, Raimondi A, Pasham M, Upadhyayula S, et al. Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling. Cell Rep. 2019;27:3049-3061.e6 pubmed publisher
	Zlatic S, Tornieri K, L hernault S, Faundez V. Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells. Mol Biol Cell. 2011;22:1699-715 pubmed publisher
	Santolini E, Puri C, Salcini A, Gagliani M, Pelicci P, Tacchetti C, et al. Numb is an endocytic protein. J Cell Biol. 2000;151:1345-52 pubmed

product information

cat no :

A4325

brand :

SIGMA

name :

Monoclonal Anti-alpha-Adaptin antibody produced in mouse

prod type :

Chemical

name suffix :

clone 100/2, ascites fluid

storage :

-20C

storage temp :

-20C

shipped in :

dry ice

syns :

Anti-AP-2

mdl no :

MFCD00212881

unspsc code :

12352203

wgk :

antibody form :

ascites fluid

application(s) :

western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex

species reactivity :

bovine, human, rat

immunogen :

AP-2 adaptor polypeptides from bovine brain.

clone :

100/2

application :

The antibody reacts with the 105 and 110 kDa alpha-subunits of adaptor complex AP-2 in bovine and rat brain preparations. It reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact alpha-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of alpha-fragments obtained by trypsin cleavage of the alpha-subunit molecules.; Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.1

more info or order :

MilliporeSigma product webpage