product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
VE-cadherin (F-8) Antibody
catalog :
sc-9989
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F-8
reactivity :
human, mouse, rat, pigs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunocytochemistry knockout validation
citations: 63
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; human; 1:200; loading ...; fig s6c
  • western blot; human; 1:200; fig 1g
Polacheck W, Kutys M, Yang J, Eyckmans J, Wu Y, Vasavada H, et al. A non-canonical Notch complex regulates adherens junctions and vascular barrier function. Nature. 2017;552:258-262 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2e
Yuan X, Duan X, Li Z, Yao B, Enhejirigala -, Song W, et al. Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo. Burns Trauma. 2022;10:tkac035 pubmed publisher
  • immunocytochemistry; human; 1:250; loading ...; fig s2a
  • western blot; human; 1:1000; loading ...; fig s2b
Fernandes H, Zonnari A, Abreu R, Aday S, Bar xe3 o M, Albino I, et al. Extracellular vesicles enriched with an endothelial cell pro-survival microRNA affects skin tissue regeneration. Mol Ther Nucleic Acids. 2022;28:307-327 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 5g
Qu K, Wang C, Huang L, Qin X, Zhang K, Zhong Y, et al. TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis. Int J Biol Sci. 2022;18:2163-2180 pubmed publisher
  • western blot; human; loading ...; fig 4f
  • immunocytochemistry; mouse; fig 4d
Peroutka R, Buzza M, Mukhopadhyay S, Johnson T, Driesbaugh K, Antalis T. Testisin/Prss21 deficiency causes increased vascular permeability and a hemorrhagic phenotype during luteal angiogenesis. PLoS ONE. 2020;15:e0234407 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2c
Carvalho J, Fortunato I, Fonseca C, Pezzarossa A, Barbacena P, Domínguez Cejudo M, et al. Non-canonical Wnt signaling regulates junctional mechanocoupling during angiogenic collective cell migration. elife. 2019;8: pubmed publisher
  • western blot; mouse; loading ...
Hancock M, Hafstad A, Nabeebaccus A, Catibog N, Logan A, Smyrnias I, et al. Myocardial NADPH oxidase-4 regulates the physiological response to acute exercise. elife. 2018;7: pubmed publisher
  • western blot; human; 1:500; fig 1c
Delgado Bellido D, Fernández Cortés M, Rodriguez M, Serrano Saenz S, Carracedo A, García Díaz A, et al. VE-cadherin promotes vasculogenic mimicry by modulating kaiso-dependent gene expression. Cell Death Differ. 2019;26:348-361 pubmed publisher
  • western blot; human; loading ...; fig 1c
  • western blot; mouse; loading ...; fig 8d
Chattopadhyay R, Raghavan S, Rao G. Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function. Redox Biol. 2017;12:438-455 pubmed publisher
  • immunohistochemistry - frozen section; pigs ; loading ...; fig 1f
Kober A, Manavalan A, Tam Amersdorfer C, Holmér A, Saeed A, Fanaee Danesh E, et al. Implications of cerebrovascular ATP-binding cassette transporter G1 (ABCG1) and apolipoprotein M in cholesterol transport at the blood-brain barrier. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862:573-588 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 5a
Smith C, Mont S, Traver G, Sekhar K, Crooks P, Freeman M. Targeting Enox1 in tumor stroma increases the efficacy of fractionated radiotherapy. Oncotarget. 2016;7:77926-77936 pubmed publisher
  • western blot; human; loading ...; fig 1a
Bruinsma W, Aprelia M, García Santisteban I, Kool J, Xu Y, Medema R. Inhibition of Polo-like kinase 1 during the DNA damage response is mediated through loss of Aurora A recruitment by Bora. Oncogene. 2017;36:1840-1848 pubmed publisher
  • western blot; human; 1:100; loading ...; fig 2b
Scotti L, Di Pietro M, Pascuali N, Irusta G, I de Zúñiga -, Gomez Peña M, et al. Sphingosine-1-phosphate restores endothelial barrier integrity in ovarian hyperstimulation syndrome. Mol Hum Reprod. 2016;22:852-866 pubmed
  • immunocytochemistry; human; 1:100; loading ...; tbl 1
Bao X, Lian X, Palecek S. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions. Methods Mol Biol. 2016;1481:183-96 pubmed publisher
  • immunocytochemistry; human; 1:25; fig 2c
Clark P, Al Ahmad A, Qian T, Zhang R, Wilson H, Weichert J, et al. Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model. Mol Pharm. 2016;13:3341-9 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Dorland Y, Malinova T, van Stalborch A, Grieve A, van Geemen D, Jansen N, et al. The F-BAR protein pacsin2 inhibits asymmetric VE-cadherin internalization from tensile adherens junctions. Nat Commun. 2016;7:12210 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 9d
Ebert L, Tan L, Johan M, Min K, Cockshell M, Parham K, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis. 2016;19:463-86 pubmed publisher
  • western blot; mouse; fig 7
Chatterjee I, Baruah J, Lurie E, Wary K. Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development. Cardiovasc Res. 2016;111:105-18 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 3b
  • western blot; human; 1:1000; loading ...; fig 1c
Choi S, Kim M, Lee H, Kim E, Kim C, Lee Y. Effects of NOX1 on fibroblastic changes of endothelial cells in radiation?induced pulmonary fibrosis. Mol Med Rep. 2016;13:4135-42 pubmed publisher
  • immunocytochemistry; rat; 1:100; fig 2
Lu W, Su L, Yu Z, Zhang S, Miao J. The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial-Like Cells. Stem Cells Int. 2016;2016:2539781 pubmed publisher
  • western blot; human; fig 1
Li J, Pan Q, Rowan P, Trotter T, Peker D, Regal K, et al. Heparanase promotes myeloma progression by inducing mesenchymal features and motility of myeloma cells. Oncotarget. 2016;7:11299-309 pubmed publisher
  • western blot; human; 1:500; fig 3
Marchi S, Corricelli M, Trapani E, Bravi L, Pittaro A, Delle Monache S, et al. Defective autophagy is a key feature of cerebral cavernous malformations. EMBO Mol Med. 2015;7:1403-17 pubmed publisher
  • immunocytochemistry; mouse
Fu Y, Huang C, Xu X, Gu H, Ye Y, Jiang C, et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails. Cell Res. 2015;25:1013-24 pubmed publisher
  • western blot; rat; 1:200; fig 6
Nishimatsu H, Suzuki E, Saito Y, Niimi A, Nomiya A, Yamada D, et al. Neuromedin B Restores Erectile Function by Protecting the Cavernous Body and the Nitrergic Nerves from Injury in a Diabetic Rat Model. PLoS ONE. 2015;10:e0133874 pubmed publisher
  • western blot; human; 1:200; fig 5
Nishimatsu H, Suzuki E, Saito Y, Niimi A, Nomiya A, Fukuhara H, et al. Senescent Cells Impair Erectile Function through Induction of Endothelial Dysfunction and Nerve Injury in Mice. PLoS ONE. 2015;10:e0124129 pubmed publisher
  • immunohistochemistry - paraffin section; pigs
Charest J, Okamoto T, Kitano K, Yasuda A, Gilpin S, Mathisen D, et al. Design and validation of a clinical-scale bioreactor for long-term isolated lung culture. Biomaterials. 2015;52:79-87 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 3
Horváth L, Umehara Y, Jud C, Blank F, Petri Fink A, Rothen Rutishauser B. Engineering an in vitro air-blood barrier by 3D bioprinting. Sci Rep. 2015;5:7974 pubmed publisher
  • immunohistochemistry - paraffin section; human
  • western blot; human; 1:200
Li Y, Zhao Y, Zou Q, Zhang K, Wu Y, Zhou C, et al. Preeclampsia does not alter vascular growth and expression of CD31 and vascular endothelial cadherin in human placentas. J Histochem Cytochem. 2015;63:22-31 pubmed publisher
  • immunocytochemistry; human
Guerrero J, Oliveira H, Catros S, Siadous R, Derkaoui S, Bareille R, et al. The use of total human bone marrow fraction in a direct three-dimensional expansion approach for bone tissue engineering applications: focus on angiogenesis and osteogenesis. Tissue Eng Part A. 2015;21:861-74 pubmed publisher
  • immunocytochemistry; human; fig 2, 3, 4
Menhofer M, Bartel D, Liebl J, Kubisch R, Busse J, Wagner E, et al. In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor. Cardiovasc Res. 2014;104:303-14 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 2
Dogan A, Demirci S, Sahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell Biol Int. 2015;39:94-103 pubmed publisher
  • immunocytochemistry; human; 1:50
Malin D, Strekalova E, Petrovic V, Deal A, Al Ahmad A, Adamo B, et al. ?B-crystallin: a novel regulator of breast cancer metastasis to the brain. Clin Cancer Res. 2014;20:56-67 pubmed publisher
  • immunocytochemistry; human
  • immunohistochemistry - frozen section; mouse
Zeng L, Wang G, Ummarino D, Margariti A, Xu Q, Xiao Q, et al. Histone deacetylase 3 unconventional splicing mediates endothelial-to-mesenchymal transition through transforming growth factor ?2. J Biol Chem. 2013;288:31853-66 pubmed publisher
Tiruppathi C, Wang D, Ansari M, Bano S, Tsukasaki Y, Mukhopadhyay A, et al. Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions. Nat Commun. 2023;14:6582 pubmed publisher
Liu J, Zhao C, Xiao X, Li A, Liu Y, Zhao J, et al. Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation. Nat Commun. 2023;14:6457 pubmed publisher
Zhao X, Kiyozuka K, Konishi A, Kawabata Iwakawa R, Minamishima Y, Obinata H. Actin-binding protein filamin B regulates the cell-surface retention of endothelial sphingosine 1-phosphate receptor 1. J Biol Chem. 2023;299:104851 pubmed publisher
Schrenk S, Bischoff L, Goines J, Cai Y, Vemaraju S, Odaka Y, et al. MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ. Nat Commun. 2023;14:1929 pubmed publisher
Suzuki S, Ando F, Kitagawa S, Hara Y, Fujiki T, Mandai S, et al. ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells. Commun Biol. 2023;6:29 pubmed publisher
Gómez Ferrer M, Amaro Prellezo E, Dorronsoro A, Sánchez Sánchez R, Vicente A, Cosin Roger J, et al. HIF-Overexpression and Pro-Inflammatory Priming in Human Mesenchymal Stromal Cells Improves the Healing Properties of Extracellular Vesicles in Experimental Crohn's Disease. Int J Mol Sci. 2021;22: pubmed publisher
Lee Y, Wang H, Su C, Lee H, Chou Y, Hsieh C, et al. Deferoxamine accelerates endothelial progenitor cell senescence and compromises angiogenesis. Aging (Albany NY). 2021;13:21364-21384 pubmed publisher
Nitzsche A, Pietilä R, Love D, Testini C, Ninchoji T, Smith R, et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis. EMBO Rep. 2021;22:e50218 pubmed publisher
Sindi H, Russomanno G, Satta S, Abdul Salam V, Jo K, Qazi Chaudhry B, et al. Therapeutic potential of KLF2-induced exosomal microRNAs in pulmonary hypertension. Nat Commun. 2020;11:1185 pubmed publisher
Chung J, Kim K, An S, Lee S, Lim B, Kang S, et al. Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress. Exp Mol Med. 2019;51:144 pubmed publisher
Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
Gu A, Kho D, Johnson R, Graham E, O Carroll S. In Vitro Wounding Models Using the Electric Cell-Substrate Impedance Sensing (ECIS)-Zθ Technology. Biosensors (Basel). 2018;8: pubmed publisher
Robilliard L, Kho D, Johnson R, Anchan A, O Carroll S, Graham E. The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier. Biosensors (Basel). 2018;8: pubmed publisher
Lakshmikanthan S, Sobczak M, Li Calzi S, Shaw L, Grant M, Chrzanowska Wodnicka M. Rap1B promotes VEGF-induced endothelial permeability and is required for dynamic regulation of the endothelial barrier. J Cell Sci. 2018;131: pubmed publisher
Baruah J, Hitzman R, Zhang J, Chaudhuri S, Mastej V, Wary K. The allosteric glycogen synthase kinase-3 inhibitor NP12 limits myocardial remodeling and promotes angiogenesis in an acute myocardial infarction model. J Biol Chem. 2017;292:20785-20798 pubmed publisher
Hirano M, Hirano K. Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci Rep. 2016;6:20989 pubmed publisher
Wu Z, Li S, Liu J, Shi Y, Wang J, Chen D, et al. RNAi-mediated silencing of AQP1 expression inhibited the proliferation, invasion and tumorigenesis of osteosarcoma cells. Cancer Biol Ther. 2015;16:1332-40 pubmed publisher
Mehta V, Abi Nader K, Shangaris P, Shaw S, Filippi E, Benjamin E, et al. Local over-expression of VEGF-D?N?C in the uterine arteries of pregnant sheep results in long-term changes in uterine artery contractility and angiogenesis. PLoS ONE. 2014;9:e100021 pubmed publisher
Galas R, Liu J. Surface density of vascular endothelial growth factor modulates endothelial proliferation and differentiation. J Cell Biochem. 2014;115:111-20 pubmed publisher
Ferro F, Spelat R, D Aurizio F, Puppato E, Pandolfi M, Beltrami A, et al. Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. PLoS ONE. 2012;7:e41774 pubmed publisher
Bevan H, Slater S, Clarke H, Cahill P, Mathieson P, Welsh G, et al. Acute laminar shear stress reversibly increases human glomerular endothelial cell permeability via activation of endothelial nitric oxide synthase. Am J Physiol Renal Physiol. 2011;301:F733-42 pubmed publisher
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Sun X, Ma S, Wade M, Flores C, Pino Yanes M, Moitra J, et al. Functional variants of the sphingosine-1-phosphate receptor 1 gene associate with asthma susceptibility. J Allergy Clin Immunol. 2010;126:241-9, 249.e1-3 pubmed publisher
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product information
Catalog Number :
sc-9989
Product Name :
VE-cadherin (F-8) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
F-8
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
VE-cadherin
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, FCM, ELISA
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA