product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Cdc6 (180.2) Antibody
catalog :
sc-9964
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
180.2
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 47
Published Application/Species/Sample/DilutionReference
  • western blot; human; fig 5d
Gotovac J, Kader T, Milne J, Fujihara K, Lara Gonzalez L, Gorringe K, et al. Loss of SMAD4 Is Sufficient to Promote Tumorigenesis in a Model of Dysplastic Barrett's Esophagus. Cell Mol Gastroenterol Hepatol. 2021;12:689-713 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 4b
Lei T, Zhang P, Zhang X, Xiao X, Zhang J, Qiu T, et al. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation. Nat Commun. 2018;9:1876 pubmed publisher
  • western blot; human; 1:20,000; loading ...; fig s2e
Matson J, Dumitru R, Coryell P, Baxley R, Chen W, Twaroski K, et al. Rapid DNA replication origin licensing protects stem cell pluripotency. elife. 2017;6: pubmed publisher
  • western blot; human; 1:1500; fig 1
Huang Y, Amin A, Qin Y, Wang Z, Jiang H, Liang L, et al. A Role of hIPI3 in DNA Replication Licensing in Human Cells. PLoS ONE. 2016;11:e0151803 pubmed publisher
  • western blot; human; 1:1000; fig s7
Passerini V, Ozeri Galai E, de Pagter M, Donnelly N, Schmalbrock S, Kloosterman W, et al. The presence of extra chromosomes leads to genomic instability. Nat Commun. 2016;7:10754 pubmed publisher
  • western blot; human; loading ...; fig 1b
Preet R, Siddharth S, Satapathy S, Das S, Nayak A, Das D, et al. Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade. Biochem Pharmacol. 2016;105:23-33 pubmed publisher
  • western blot; human; fig 8
Yao M, Xie C, Kiang M, Teng Y, Harman D, Tiffen J, et al. Targeting of cytosolic phospholipase A2α impedes cell cycle re-entry of quiescent prostate cancer cells. Oncotarget. 2015;6:34458-74 pubmed publisher
  • western blot; mouse; 1:200; fig 2
Cardona M, López J, Serafín A, Rongvaux A, Inserte J, García Dorado D, et al. Executioner Caspase-3 and 7 Deficiency Reduces Myocyte Number in the Developing Mouse Heart. PLoS ONE. 2015;10:e0131411 pubmed publisher
  • western blot; human; fig 2a
Wu M, Lu W, Santos R, Frattini M, Kelly T. Geminin inhibits a late step in the formation of human pre-replicative complexes. J Biol Chem. 2014;289:30810-21 pubmed publisher
  • western blot; human; 1:500; fig 1
Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher
  • western blot; human; fig 2e
Wang J, Xu X, Alontaga A, Chen Y, Liu Y. Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction. Cell Rep. 2014;7:848-58 pubmed publisher
  • western blot; human; 1:1000; fig 2
Clijsters L, Wolthuis R. PIP-box-mediated degradation prohibits re-accumulation of Cdc6 during S phase. J Cell Sci. 2014;127:1336-45 pubmed publisher
  • western blot; human
Wei Q, Li J, Liu T, Tong X, Ye X. Phosphorylation of minichromosome maintenance protein 7 (MCM7) by cyclin/cyclin-dependent kinase affects its function in cell cycle regulation. J Biol Chem. 2013;288:19715-25 pubmed publisher
  • western blot; human; 1:1000
Benatti P, Dolfini D, Vigano A, Ravo M, Weisz A, Imbriano C. Specific inhibition of NF-Y subunits triggers different cell proliferation defects. Nucleic Acids Res. 2011;39:5356-68 pubmed publisher
Barua D, Sultana A, Islam M, Cox F, Gupta A, Gupta S. RRM2 and CDC6 are novel effectors of XBP1-mediated endocrine resistance and predictive markers of tamoxifen sensitivity. BMC Cancer. 2023;23:288 pubmed publisher
Kumar R, Rao G. Novel Role of Prereplication Complex Component Cell Division Cycle 6 in Retinal Neovascularization. Arterioscler Thromb Vasc Biol. 2022;42:407-427 pubmed publisher
Enrico T, Stallaert W, Wick E, Ngoi P, Wang X, Rubin S, et al. Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2. elife. 2021;10: pubmed publisher
van Schie J, Faramarz A, Balk J, Stewart G, Cantelli E, Oostra A, et al. Warsaw Breakage Syndrome associated DDX11 helicase resolves G-quadruplex structures to support sister chromatid cohesion. Nat Commun. 2020;11:4287 pubmed publisher
Matson J, House A, Grant G, Wu H, Perez J, Cook J. Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence. J Cell Biol. 2019;: pubmed publisher
Lemmens B, Hegarat N, Akopyan K, Sala Gaston J, Bartek J, Hochegger H, et al. DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Mol Cell. 2018;71:117-128.e3 pubmed publisher
Patel P, Suram A, Mirani N, Bischof O, Herbig U. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc Natl Acad Sci U S A. 2016;113:E5024-33 pubmed publisher
Kliszczak M, Sedlackova H, Pitchai G, Streicher W, Krejci L, Hickson I. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget. 2015;6:40464-79 pubmed publisher
Kalfalah F, Berg E, Christensen M, Linka R, Dirks W, Boege F, et al. Spatio-temporal regulation of the human licensing factor Cdc6 in replication and mitosis. Cell Cycle. 2015;14:1704-15 pubmed publisher
Clijsters L, Ogink J, Wolthuis R. The spindle checkpoint, APC/C(Cdc20), and APC/C(Cdh1) play distinct roles in connecting mitosis to S phase. J Cell Biol. 2013;201:1013-26 pubmed publisher
Booher K, Lin D, Borrego S, Kaiser P. Downregulation of Cdc6 and pre-replication complexes in response to methionine stress in breast cancer cells. Cell Cycle. 2012;11:4414-23 pubmed publisher
Moriyama K, Yoshizawa Sugata N, Obuse C, Tsurimoto T, Masai H. Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1. J Biol Chem. 2012;287:23977-94 pubmed publisher
Varma D, Chandrasekaran S, Sundin L, Reidy K, Wan X, Chasse D, et al. Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment. Nat Cell Biol. 2012;14:593-603 pubmed publisher
Isaacs J, D Antonio J, Chen S, Antony L, Dalrymple S, Ndikuyeze G, et al. Adaptive auto-regulation of androgen receptor provides a paradigm shifting rationale for bipolar androgen therapy (BAT) for castrate resistant human prostate cancer. Prostate. 2012;72:1491-505 pubmed publisher
Yao M, Xie C, Constantine M, Hua S, Hambly B, Jardine G, et al. How can food extracts consumed in the Mediterranean and East Asia suppress prostate cancer proliferation?. Br J Nutr. 2012;108:424-30 pubmed publisher
Chandrasekaran S, Tan T, Hall J, Cook J. Stress-stimulated mitogen-activated protein kinases control the stability and activity of the Cdt1 DNA replication licensing factor. Mol Cell Biol. 2011;31:4405-16 pubmed publisher
Li C, Nyman J, Braithwaite A, Eccles M. PAX8 promotes tumor cell growth by transcriptionally regulating E2F1 and stabilizing RB protein. Oncogene. 2011;30:4824-34 pubmed publisher
Munkley J, Copeland N, Moignard V, Knight J, Greaves E, Ramsbottom S, et al. Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Res. 2011;39:2671-7 pubmed publisher
Zhang J, Yu L, Wu X, Zou L, Sou K, Wei Z, et al. The interacting domains of hCdt1 and hMcm6 involved in the chromatin loading of the MCM complex in human cells. Cell Cycle. 2010;9:4848-57 pubmed
Ladstein R, Bachmann I, Straume O, Akslen L. Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma. BMC Cancer. 2010;10:140 pubmed publisher
Eliades A, Papadantonakis N, Ravid K. New roles for cyclin E in megakaryocytic polyploidization. J Biol Chem. 2010;285:18909-17 pubmed publisher
Di Paola D, Rampakakis E, Chan M, Arvanitis D, Zannis Hadjopoulos M. Increased origin activity in transformed versus normal cells: identification of novel protein players involved in DNA replication and cellular transformation. Nucleic Acids Res. 2010;38:2314-31 pubmed publisher
Lu X, Liu J, Legerski R. Cyclin E is stabilized in response to replication fork barriers leading to prolonged S phase arrest. J Biol Chem. 2009;284:35325-37 pubmed publisher
Gan F, Chua Y, Scarmagnani S, Palaniappan P, Franks M, Poobalasingam T, et al. Structure-activity analysis of 2'-modified cinnamaldehyde analogues as potential anticancer agents. Biochem Biophys Res Commun. 2009;387:741-7 pubmed publisher
Agherbi H, Gaussmann Wenger A, Verthuy C, Chasson L, Serrano M, Djabali M. Polycomb mediated epigenetic silencing and replication timing at the INK4a/ARF locus during senescence. PLoS ONE. 2009;4:e5622 pubmed publisher
Nevis K, Cordeiro Stone M, Cook J. Origin licensing and p53 status regulate Cdk2 activity during G(1). Cell Cycle. 2009;8:1952-63 pubmed
Salabat M, Melstrom L, Strouch M, Ding X, Milam B, Ujiki M, et al. Geminin is overexpressed in human pancreatic cancer and downregulated by the bioflavanoid apigenin in pancreatic cancer cell lines. Mol Carcinog. 2008;47:835-44 pubmed publisher
Barkley L, Hong H, Kingsbury S, James M, Stoeber K, Williams G. Cdc6 is a rate-limiting factor for proliferative capacity during HL60 cell differentiation. Exp Cell Res. 2007;313:3789-99 pubmed
Chew E, Poobalasingam T, Hawkey C, Hagen T. Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells--evidence for cullin dimerization. Cell Signal. 2007;19:1071-80 pubmed
Vassilev L, Tovar C, Chen S, Knezevic D, Zhao X, Sun H, et al. Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc Natl Acad Sci U S A. 2006;103:10660-5 pubmed
Fujii Yamamoto H, Kim J, Arai K, Masai H. Cell cycle and developmental regulations of replication factors in mouse embryonic stem cells. J Biol Chem. 2005;280:12976-87 pubmed
Anger M, Stein P, Schultz R. CDC6 requirement for spindle formation during maturation of mouse oocytes. Biol Reprod. 2005;72:188-94 pubmed
Martins S, Eikvar S, Furukawa K, Collas P. HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication. J Cell Biol. 2003;160:177-88 pubmed
product information
Catalog Number :
sc-9964
Product Name :
Cdc6 (180.2) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
180.2
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
Cdc6
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, IHC(P)
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA