product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
NFkappaB p50 (E-10) Antibody
catalog :
sc-8414
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E-10
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 70
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; fig 7b
Botto S, Abraham J, Mizuno N, Pryke K, Gall B, Landais I, et al. Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome. MBio. 2019;10: pubmed publisher
  • western blot; mouse; loading ...; fig 2d
Cong Q, Jia H, Li P, Qiu S, Yeh J, Wang Y, et al. p38α MAPK regulates proliferation and differentiation of osteoclast progenitors and bone remodeling in an aging-dependent manner. Sci Rep. 2017;7:45964 pubmed publisher
  • western blot; human; loading ...; fig 5b
Labrousse Arias D, Martínez Alonso E, Corral Escariz M, Bienes Martínez R, Berridy J, Serrano Oviedo L, et al. VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1. J Cell Biol. 2017;216:835-847 pubmed publisher
  • western blot; mouse; loading ...; fig 6b
Rackov G, Hernandez Jimenez E, Shokri R, Carmona Rodríguez L, Manes S, Alvarez Mon M, et al. p21 mediates macrophage reprogramming through regulation of p50-p50 NF-?B and IFN-?. J Clin Invest. 2016;126:3089-103 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 9a
  • western blot; mouse; loading ...; fig 9d
Shen Z, Liu Y, Dewidar B, Hu J, Park O, Feng T, et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. Am J Pathol. 2016;186:1874-1889 pubmed publisher
  • western blot; mouse; fig 6
Lee H, Sanada S, An S, Ye B, Lee J, Seo Y, et al. LPS-induced NF?B enhanceosome requires TonEBP/NFAT5 without DNA binding. Sci Rep. 2016;6:24921 pubmed publisher
  • western blot; human; fig 6b
Urban B, Collard T, Eagle C, Southern S, Greenhough A, Hamdollah Zadeh M, et al. BCL-3 expression promotes colorectal tumorigenesis through activation of AKT signalling. Gut. 2016;65:1151-64 pubmed publisher
  • western blot; human; 1:1000
Lan N, Luo G, Yang X, Cheng Y, Zhang Y, Wang X, et al. 25-Hydroxyvitamin D3-deficiency enhances oxidative stress and corticosteroid resistance in severe asthma exacerbation. PLoS ONE. 2014;9:e111599 pubmed publisher
  • western blot; human
Brenne A, Fagerli U, Shaughnessy J, Våtsveen T, Rø T, Hella H, et al. High expression of BCL3 in human myeloma cells is associated with increased proliferation and inferior prognosis. Eur J Haematol. 2009;82:354-63 pubmed publisher
  • chromatin immunoprecipitation; human
Gao Z, Chiao P, Zhang X, Zhang X, Lazar M, Seto E, et al. Coactivators and corepressors of NF-kappaB in IkappaB alpha gene promoter. J Biol Chem. 2005;280:21091-8 pubmed
  • western blot; mouse
Chen F, Harrison L. Ciglitazone induces early cellular proliferation and NF-kappaB transcriptional activity in colon cancer cells through p65 phosphorylation. Int J Biochem Cell Biol. 2005;37:645-54 pubmed
Jiang W, Yan Y, Chen M, Luo G, Hao J, Pan J, et al. Aspirin enhances the sensitivity of colon cancer cells to cisplatin by abrogating the binding of NF-κB to the COX-2 promoter. Aging (Albany NY). 2020;12:611-627 pubmed publisher
Waters M, Gupta A, Mockenhaupt K, Brown L, Biswas D, Kordula T. RelB acts as a molecular switch driving chronic inflammation in glioblastoma multiforme. Oncogenesis. 2019;8:37 pubmed publisher
Gupta A, Waters M, Biswas D, Brown L, Surace M, Floros C, et al. RelB controls adaptive responses of astrocytes during sterile inflammation. Glia. 2019;67:1449-1461 pubmed publisher
Choi E, Ren Y, Chen Y, Liu S, Wu W, Ren J, et al. Exchange Proteins Directly Activated by cAMP and Their Roles in Respiratory Syncytial Virus Infection. J Virol. 2018;92: pubmed publisher
Reißig S, Tang Y, Nikolaev A, Gerlach K, Wolf C, Davari K, et al. Elevated levels of Bcl-3 inhibits Treg development and function resulting in spontaneous colitis. Nat Commun. 2017;8:15069 pubmed publisher
Srinivasan S, Su M, Ravishankar S, Moore J, Head P, Dixon J, et al. TLR-exosomes exhibit distinct kinetics and effector function. Sci Rep. 2017;7:41623 pubmed publisher
Zhang X, Shi Y, Weng Y, Lai Q, Luo T, Zhao J, et al. The truncate mutation of Notch2 enhances cell proliferation through activating the NF-κB signal pathway in the diffuse large B-cell lymphomas. PLoS ONE. 2014;9:e108747 pubmed publisher
Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks S, Gunnarsson J, et al. MALT1 auto-proteolysis is essential for NF-κB-dependent gene transcription in activated lymphocytes. PLoS ONE. 2014;9:e103774 pubmed publisher
Voce D, Schmitt A, Uppal A, McNerney M, Bernal G, Cahill K, et al. Nfkb1 is a haploinsufficient DNA damage-specific tumor suppressor. Oncogene. 2015;34:2807-13 pubmed publisher
Zhang J, Herreros Villanueva M, Herreros Vilanueva M, Koenig A, Deng Z, de Narvajas A, et al. Differential activity of GSK-3 isoforms regulates NF-?B and TRAIL- or TNF? induced apoptosis in pancreatic cancer cells. Cell Death Dis. 2014;5:e1142 pubmed publisher
Morales Cano D, Calviño E, Rubio V, Herráez A, Sancho P, Tejedor M, et al. Apoptosis induced by paclitaxel via Bcl-2, Bax and caspases 3 and 9 activation in NB4 human leukaemia cells is not modulated by ERK inhibition. Exp Toxicol Pathol. 2013;65:1101-8 pubmed publisher
Longoni N, Sarti M, Albino D, Civenni G, Malek A, Ortelli E, et al. ETS transcription factor ESE1/ELF3 orchestrates a positive feedback loop that constitutively activates NF-?B and drives prostate cancer progression. Cancer Res. 2013;73:4533-47 pubmed publisher
Kwak S, Jeong M, Choi J, Kim D, Min H, Yoon Y, et al. Amelioration of behavioral abnormalities in BH(4)-deficient mice by dietary supplementation of tyrosine. PLoS ONE. 2013;8:e60803 pubmed publisher
Nakamura H, Aoki K, Masuda W, Alles N, Nagano K, Fukushima H, et al. Disruption of NF-?B1 prevents bone loss caused by mechanical unloading. J Bone Miner Res. 2013;28:1457-67 pubmed publisher
Yang H, Kim J, Kim S, Choe C, Jang Y. Pro-IL-16 is associated with MHC class II-mediated negative regulation of mouse resting B cell activation through MAP kinases, NF-?B and Skp2-dependent p27kip regulation. Scand J Immunol. 2013;77:177-86 pubmed publisher
Tsutsuki H, Yahiro K, Suzuki K, Suto A, Ogura K, Nagasawa S, et al. Subtilase cytotoxin enhances Escherichia coli survival in macrophages by suppression of nitric oxide production through the inhibition of NF-?B activation. Infect Immun. 2012;80:3939-51 pubmed publisher
Iofrida C, Melissari E, Mariotti V, Guglielmi C, Guidugli L, Caligo M, et al. Effects on human transcriptome of mutated BRCA1 BRCT domain: a microarray study. BMC Cancer. 2012;12:207 pubmed publisher
Huang T, Chang W, Wang M, Yang Y, Hsu Y. Effect of sulforaphane on growth inhibition in human brain malignant glioma GBM 8401 cells by means of mitochondrial- and MEK/ERK-mediated apoptosis pathway. Cell Biochem Biophys. 2012;63:247-59 pubmed publisher
Huang T, Hsu C, Chang W, Wang M, Wu J, Hsu Y. Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells. Evid Based Complement Alternat Med. 2012;2012:396573 pubmed publisher
Kliem C, Merling A, Giaisi M, Kohler R, Krammer P, Li Weber M. Curcumin suppresses T cell activation by blocking Ca2+ mobilization and nuclear factor of activated T cells (NFAT) activation. J Biol Chem. 2012;287:10200-9 pubmed publisher
Herkenham M, Rathore P, Brown P, Listwak S. Cautionary notes on the use of NF-?B p65 and p50 antibodies for CNS studies. J Neuroinflammation. 2011;8:141 pubmed publisher
Southern S, Collard T, Urban B, Skeen V, Smartt H, Hague A, et al. BAG-1 interacts with the p50-p50 homodimeric NF-?B complex: implications for colorectal carcinogenesis. Oncogene. 2012;31:2761-72 pubmed publisher
Chen M, Tang W, Hsu C, Tsai Y, Wu J, Lin C, et al. Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane. Evid Based Complement Alternat Med. 2012;2012:415231 pubmed publisher
Kwak S, Suk J, Choi J, Yang S, Kim J, Sohn S, et al. Autophagy induction by tetrahydrobiopterin deficiency. Autophagy. 2011;7:1323-34 pubmed publisher
Della Chiara G, Crotti A, Liboi E, Giacca M, Poli G, Lusic M. Negative regulation of HIV-1 transcription by a heterodimeric NF-?B1/p50 and C-terminally truncated STAT5 complex. J Mol Biol. 2011;410:933-43 pubmed publisher
Toscano M, Campagna L, Molinero L, Cerliani J, Croci D, Ilarregui J, et al. Nuclear factor (NF)-?B controls expression of the immunoregulatory glycan-binding protein galectin-1. Mol Immunol. 2011;48:1940-9 pubmed publisher
Chen J, Liu D, Bai Q, Song J, Guan J, Gao J, et al. Celecoxib attenuates liver steatosis and inflammation in non-alcoholic steatohepatitis induced by high-fat diet in rats. Mol Med Rep. 2011;4:811-6 pubmed publisher
Gizard F, Heywood E, Findeisen H, Zhao Y, Jones K, Cudejko C, et al. Telomerase activation in atherosclerosis and induction of telomerase reverse transcriptase expression by inflammatory stimuli in macrophages. Arterioscler Thromb Vasc Biol. 2011;31:245-52 pubmed publisher
Gomes N, Brunialti M, Mendes M, Freudenberg M, Galanos C, Salomao R. Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood. Braz J Med Biol Res. 2010;43:853-8 pubmed
Kuliková L, Mikes J, Hyzdalova M, Palumbo G, Fedorocko P. NF-?B is not directly responsible for photoresistance induced by fractionated light delivery in HT-29 colon adenocarcinoma cells. Photochem Photobiol. 2010;86:1285-93 pubmed publisher
Kim K, Kim Y, Koh B, Hwang S, Kim S, Lepine F, et al. HHQ and PQS, two Pseudomonas aeruginosa quorum-sensing molecules, down-regulate the innate immune responses through the nuclear factor-kappaB pathway. Immunology. 2010;129:578-88 pubmed publisher
Rodella L, Filippini F, Bonomini F, Bresciani R, Reiter R, Rezzani R. Beneficial effects of melatonin on nicotine-induced vasculopathy. J Pineal Res. 2010;48:126-32 pubmed publisher
Yang H, Kim J, Lee K, Jang Y. Rac/ROS-related protein kinase C and phosphatidylinositol-3-kinase signaling are involved in a negative regulating cascade in B cell activation by antibody-mediated cross-linking of MHC class II molecules. Mol Immunol. 2010;47:706-12 pubmed publisher
Maruyama T, Fukushima H, Nakao K, Shin M, Yasuda H, Weih F, et al. Processing of the NF-kappa B2 precursor p100 to p52 is critical for RANKL-induced osteoclast differentiation. J Bone Miner Res. 2010;25:1058-67 pubmed publisher
Rhoads M, Kandarian S, Pacelli F, Doglietto G, Bossola M. Expression of NF-kappaB and IkappaB proteins in skeletal muscle of gastric cancer patients. Eur J Cancer. 2010;46:191-7 pubmed publisher
Schmuckli Maurer J, Kinnaird J, Pillai S, Hermann P, McKellar S, Weir W, et al. Modulation of NF-kappaB activation in Theileria annulata-infected cloned cell lines is associated with detection of parasite-dependent IKK signalosomes and disruption of the actin cytoskeleton. Cell Microbiol. 2010;12:158-73 pubmed publisher
Olsnes A, Ersvaer E, Ryningen A, Paulsen K, Hampson P, Lord J, et al. The protein kinase C agonist PEP005 increases NF-kappaB expression, induces differentiation and increases constitutive chemokine release by primary acute myeloid leukaemia cells. Br J Haematol. 2009;145:761-74 pubmed publisher
Choi Y, Hur J, Lee H, Jeong S. The RNA aptamer disrupts protein-protein interaction between beta-catenin and nuclear factor-kappaB p50 and regulates the expression of C-reactive protein. FEBS Lett. 2009;583:1415-21 pubmed publisher
Kim M, Rajapakse N, Kim S. Anti-inflammatory effect of Ishige okamurae ethanolic extract via inhibition of NF-kappaB transcription factor in RAW 264.7 cells. Phytother Res. 2009;23:628-34 pubmed publisher
Paz Priel I, Ghosal A, Kowalski J, Friedman A. C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters. Leukemia. 2009;23:365-74 pubmed publisher
Fiorillo C, Becatti M, Pensalfini A, Cecchi C, Lanzilao L, Donzelli G, et al. Curcumin protects cardiac cells against ischemia-reperfusion injury: effects on oxidative stress, NF-kappaB, and JNK pathways. Free Radic Biol Med. 2008;45:839-46 pubmed publisher
Yang Y, Lu N, Zhou J, Chen Z, Zhu P. Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis. Rheumatology (Oxford). 2008;47:1299-310 pubmed publisher
McMillan R, Sikes M. Differential activation of dual promoters alters Dbeta2 germline transcription during thymocyte development. J Immunol. 2008;180:3218-28 pubmed
Notebaert S, Demon D, Vanden Berghe T, Vandenabeele P, Meyer E. Inflammatory mediators in Escherichia coli-induced mastitis in mice. Comp Immunol Microbiol Infect Dis. 2008;31:551-65 pubmed publisher
Mendis E, Kim M, Rajapakse N, Kim S. Sulfated glucosamine inhibits oxidation of biomolecules in cells via a mechanism involving intracellular free radical scavenging. Eur J Pharmacol. 2008;579:74-85 pubmed
Golks A, Tran T, Goetschy J, Guerini D. Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation. EMBO J. 2007;26:4368-79 pubmed
Choi Y, Hur J, Jeong S. Beta-catenin binds to the downstream region and regulates the expression C-reactive protein gene. Nucleic Acids Res. 2007;35:5511-9 pubmed
Lambert C, Li J, Jonscher K, Yang T, Reigan P, Quintana M, et al. Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain. J Biol Chem. 2007;282:19666-75 pubmed
Momose I, Iijima M, Kawada M, Ikeda D. A new proteasome inhibitor, TP-110, induces apoptosis in human prostate cancer PC-3 cells. Biosci Biotechnol Biochem. 2007;71:1036-43 pubmed
Chiu J, Chen L, Lee C, Lee P, Lee D, Tsai M, et al. Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress. Blood. 2007;110:519-28 pubmed
Hafeez B, Ahmed S, Wang N, Gupta S, Zhang A, Haqqi T. Green tea polyphenols-induced apoptosis in human osteosarcoma SAOS-2 cells involves a caspase-dependent mechanism with downregulation of nuclear factor-kappaB. Toxicol Appl Pharmacol. 2006;216:11-9 pubmed
Palao G, Santiago B, Galindo M, Rullas J, Alcami J, Ramirez J, et al. Fas activation of a proinflammatory program in rheumatoid synoviocytes and its regulation by FLIP and caspase 8 signaling. Arthritis Rheum. 2006;54:1473-81 pubmed
Vacca A, Felli M, Palermo R, Di Mario G, Calce A, Di Giovine M, et al. Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia. EMBO J. 2006;25:1000-8 pubmed
Li Weber M, Treiber M, Giaisi M, Palfi K, Stephan N, Parg S, et al. Ultraviolet irradiation suppresses T cell activation via blocking TCR-mediated ERK and NF-kappa B signaling pathways. J Immunol. 2005;175:2132-43 pubmed
Wang J, Li T, Lu Z. A method for fabricating uni-dsDNA microarray chip for analyzing DNA-binding proteins. J Biochem Biophys Methods. 2005;63:100-10 pubmed
Notebaert S, Duchateau L, Meyer E. NF-kappaB inhibition accelerates apoptosis of bovine neutrophils. Vet Res. 2005;36:229-40 pubmed
Kimura M, Hosokawa H, Yamashita M, Hasegawa A, Iwamura C, Watarai H, et al. Regulation of T helper type 2 cell differentiation by murine Schnurri-2. J Exp Med. 2005;201:397-408 pubmed
Riggins R, Zwart A, Nehra R, Clarke R. The nuclear factor kappa B inhibitor parthenolide restores ICI 182,780 (Faslodex; fulvestrant)-induced apoptosis in antiestrogen-resistant breast cancer cells. Mol Cancer Ther. 2005;4:33-41 pubmed
Cherukuri D, Goulet A, Inoue H, Nelson M. Selenomethionine regulates cyclooxygenase-2 (COX-2) expression through nuclear factor-kappa B (NF-kappaB) in colon cancer cells. Cancer Biol Ther. 2005;4:175-80 pubmed
product information
Product Name :
NFkappaB p50 (E-10) Antibody
Gene Name :
NFKB1
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-8414
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
NFkappaB p50
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
120-239 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA