product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Chk1 (G-4) Antibody
catalog :
sc-8408
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
G-4
reactivity :
African green monkey, human, mouse, rat, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, reverse phase protein lysate microarray, other, western blot knockout validation
citations: 187
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 3
Niida H, Katsuno Y, Banerjee B, Hande M, Nakanishi M. Specific role of Chk1 phosphorylations in cell survival and checkpoint activation. Mol Cell Biol. 2007;27:2572-81 pubmed
  • western blot; human; 1:200; loading ...; fig 13e
Barger C, Chee L, Albahrani M, Munoz Trujillo C, Boghean L, Branick C, et al. Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer. elife. 2021;10: pubmed publisher
  • western blot; human; 1:100; loading ...; fig 1a, 1b
Ho K, Luo H, Zhu W, Tang Y. Critical role of SMG7 in activation of the ATR-CHK1 axis in response to genotoxic stress. Sci Rep. 2021;11:7502 pubmed publisher
  • western blot; human; loading ...; fig 4a
Ditano J, Donahue K, Tafe L, McCleery C, Eastman A. Sensitivity of cells to ATR and CHK1 inhibitors requires hyperactivation of CDK2 rather than endogenous replication stress or ATM dysfunction. Sci Rep. 2021;11:7077 pubmed publisher
  • western blot; mouse; loading ...; fig s2c
Sewastianik T, Straubhaar J, Zhao J, Samur M, Adler K, Tanton H, et al. miR-15a/16-1 deletion in activated B cells promotes plasma cell and mature B-cell neoplasms. Blood. 2021;137:1905-1919 pubmed publisher
  • western blot; mouse; loading ...; fig s3a
Wang Y, Luo M, Chen Y, Wang Y, Zhang B, Ren Z, et al. ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth. Cancer Res. 2021;81:174-186 pubmed publisher
  • western blot; human; loading ...; fig 7d
Pothuraju R, Rachagani S, Krishn S, Chaudhary S, Nimmakayala R, Siddiqui J, et al. Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance. Mol Cancer. 2020;19:37 pubmed publisher
  • western blot; human; 1:500; loading ...; fig s2d
Sonego M, Pellarin I, Costa A, Vinciguerra G, Coan M, Kraut A, et al. USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Sci Adv. 2019;5:eaav3235 pubmed publisher
  • western blot; human; loading ...; fig 1a
Li S, Lavagnino Z, Lemaçon D, Kong L, Ustione A, Ng X, et al. Ca2+-Stimulated AMPK-Dependent Phosphorylation of Exo1 Protects Stressed Replication Forks from Aberrant Resection. Mol Cell. 2019;74:1123-1137.e6 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 4e
Kim D, Kim S, Oh Y, Park S, Jeon Y, Kim H, et al. AIMP3 Deletion Induces Acute Radiation Syndrome-like Phenotype in Mice. Sci Rep. 2018;8:15025 pubmed publisher
  • western blot; human; loading ...; fig s15a
Saldivar J, Hamperl S, Bocek M, Chung M, Bass T, Cisneros Soberanis F, et al. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018;361:806-810 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Mirman Z, Lottersberger F, Takai H, Kibe T, Gong Y, Takai K, et al. 53BP1-RIF1-shieldin counteracts DSB resection through CST- and Polα-dependent fill-in. Nature. 2018;560:112-116 pubmed publisher
  • western blot; human; loading ...; fig 6f
Huang T, Fowler F, Chen C, Shen Z, SLECKMAN B, Tyler J. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell. 2018;69:879-892.e5 pubmed publisher
  • western blot; human; fig s10
Okazaki A, Gameiro P, Christodoulou D, Laviollette L, Schneider M, Chaves F, et al. Glutaminase and poly(ADP-ribose) polymerase inhibitors suppress pyrimidine synthesis and VHL-deficient renal cancers. J Clin Invest. 2017;127:1631-1645 pubmed publisher
  • western blot; human; loading ...; fig 2g
Leung J, Makharashvili N, Agarwal P, Chiu L, Pourpre R, Cammarata M, et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes Dev. 2017;31:260-274 pubmed publisher
  • western blot; human; loading ...; fig 2c
Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s4c
Xu H, Di Antonio M, McKinney S, Mathew V, Ho B, O Neil N, et al. CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours. Nat Commun. 2017;8:14432 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 2A
  • western blot; human; 1:500; loading ...; fig 1D; 2G
Tu Y, Liu H, Zhu X, Shen H, Ma X, Wang F, et al. Ataxin-3 promotes genome integrity by stabilizing Chk1. Nucleic Acids Res. 2017;45:4532-4549 pubmed publisher
  • western blot; mouse; loading ...; fig 7a
  • western blot; human; loading ...; fig 2a
Gong Y, Handa N, Kowalczykowski S, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev. 2017;31:46-58 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig S1D
Zanini I, Soneson C, Lorenzi L, Azzalin C. Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion. J Cell Sci. 2017;130:767-778 pubmed publisher
  • western blot; human; 1:500; loading ...; fig s6a
Zhang H, Sun L, Wang K, Wu D, Trappio M, Witting C, et al. Loss of H3K9me3 Correlates with ATM Activation and Histone H2AX Phosphorylation Deficiencies in Hutchinson-Gilford Progeria Syndrome. PLoS ONE. 2016;11:e0167454 pubmed publisher
  • western blot; human; loading ...; fig s5b
Murai J, Feng Y, Yu G, Ru Y, Tang S, Shen Y, et al. Resistance to PARP inhibitors by SLFN11 inactivation can be overcome by ATR inhibition. Oncotarget. 2016;7:76534-76550 pubmed publisher
  • western blot; human; fig 9c
Kehrli K, Phelps M, Lazarchuk P, Chen E, Monnat R, Sidorova J. Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest. J Biol Chem. 2016;291:24487-24503 pubmed
  • western blot; mouse; 1:200; fig s1
Yang C, Suzuki M, Yamakawa S, Uno S, Ishii A, Yamazaki S, et al. Claspin recruits Cdc7 kinase for initiation of DNA replication in human cells. Nat Commun. 2016;7:12135 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 5b
Adriaens C, Standaert L, Barra J, Latil M, Verfaillie A, Kalev P, et al. p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity. Nat Med. 2016;22:861-8 pubmed publisher
  • western blot; human; 1:800; fig 3
Ikeuchi M, Fukumoto Y, Honda T, Kuga T, Saito Y, Yamaguchi N, et al. v-Src Causes Chromosome Bridges in a Caffeine-Sensitive Manner by Generating DNA Damage. Int J Mol Sci. 2016;17: pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Brosh R, Hrynyk I, Shen J, Waghray A, Zheng N, Lemischka I. A dual molecular analogue tuner for dissecting protein function in mammalian cells. Nat Commun. 2016;7:11742 pubmed publisher
  • western blot; human; 1:500; loading ...; fig s5a
Zhang Y, Lai J, Du Z, Gao J, Yang S, Gorityala S, et al. Targeting radioresistant breast cancer cells by single agent CHK1 inhibitor via enhancing replication stress. Oncotarget. 2016;7:34688-702 pubmed publisher
  • western blot; human; fig 5
Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  • western blot; human; fig 2
Ribeyre C, Zellweger R, Chauvin M, Bec N, Larroque C, Lopes M, et al. Nascent DNA Proteomics Reveals a Chromatin Remodeler Required for Topoisomerase I Loading at Replication Forks. Cell Rep. 2016;15:300-9 pubmed publisher
  • western blot; human; fig 1a
Kemp M, Sancar A. ATR Kinase Inhibition Protects Non-cycling Cells from the Lethal Effects of DNA Damage and Transcription Stress. J Biol Chem. 2016;291:9330-42 pubmed publisher
  • western blot; human; fig 6
Cekan P, Hasegawa K, Pan Y, Tubman E, Odde D, Chen J, et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol Biol Cell. 2016;27:1346-57 pubmed publisher
  • western blot; human; 1:2000; fig 6
Soriano A, París Coderch L, Jubierre L, Martínez A, Zhou X, Piskareva O, et al. MicroRNA-497 impairs the growth of chemoresistant neuroblastoma cells by targeting cell cycle, survival and vascular permeability genes. Oncotarget. 2016;7:9271-87 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig st3
Nassour J, Martien S, Martin N, Deruy E, Tomellini E, Malaquin N, et al. Defective DNA single-strand break repair is responsible for senescence and neoplastic escape of epithelial cells. Nat Commun. 2016;7:10399 pubmed publisher
  • western blot; human; fig 1
Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • western blot; human; fig 2d
Baude A, Aaes T, Zhai B, Al Nakouzi N, Oo H, Daugaard M, et al. Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Nucleic Acids Res. 2016;44:2214-26 pubmed publisher
  • western blot; human; fig 3
Hoffmann S, Smedegaard S, Nakamura K, Mortuza G, Räschle M, Ibañez de Opakua A, et al. TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress. J Cell Biol. 2016;212:63-75 pubmed publisher
  • immunoprecipitation; mouse; fig 5
  • western blot; mouse; fig 5
Su X, Yan H, Huang Y, Yun H, Zeng B, Wang E, et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep. 2015;5:18588 pubmed publisher
  • western blot; human; 1:1000; fig 4
Harley M, Murina O, Leitch A, Higgs M, Bicknell L, Yigit G, et al. TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nat Genet. 2016;48:36-43 pubmed publisher
  • western blot; human; fig 1
Funauchi Y, Tanikawa C, Yi Lo P, Mori J, Daigo Y, Takano A, et al. Regulation of iron homeostasis by the p53-ISCU pathway. Sci Rep. 2015;5:16497 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5d
Kanu N, Zhang T, Burrell R, Chakraborty A, Cronshaw J, DaCosta C, et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene. 2016;35:4009-19 pubmed publisher
  • western blot; human; fig s5
Graindorge D, Martineau S, Machon C, Arnoux P, Guitton J, Francesconi S, et al. Singlet Oxygen-Mediated Oxidation during UVA Radiation Alters the Dynamic of Genomic DNA Replication. PLoS ONE. 2015;10:e0140645 pubmed publisher
  • western blot; mouse; fig 4a
Meyer S, Krebs S, Thirion C, Blum H, Krause S, Pfaffl M. Tumor Necrosis Factor Alpha and Insulin-Like Growth Factor 1 Induced Modifications of the Gene Expression Kinetics of Differentiating Skeletal Muscle Cells. PLoS ONE. 2015;10:e0139520 pubmed publisher
  • western blot; human; fig 3
Castella M, Jacquemont C, Thompson E, Yeo J, Cheung R, Huang J, et al. FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2. PLoS Genet. 2015;11:e1005563 pubmed publisher
  • western blot; human; 1:500; fig s5
Lee K, Im J, Shibata E, Park J, Handa N, Kowalczykowski S, et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun. 2015;6:7744 pubmed publisher
  • western blot; human
Kiyonari S, Iimori M, Matsuoka K, Watanabe S, Morikawa Ichinose T, Miura D, et al. The 1,2-Diaminocyclohexane Carrier Ligand in Oxaliplatin Induces p53-Dependent Transcriptional Repression of Factors Involved in Thymidylate Biosynthesis. Mol Cancer Ther. 2015;14:2332-42 pubmed publisher
  • western blot; human; 1:1000; fig 9
Simpson D, Lemonie N, Morgan D, Gaddameedhi S, Kaufmann W. Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity. Cancers (Basel). 2015;7:1072-90 pubmed publisher
  • western blot; human; fig s3e
Petroni M, Sardina F, Heil C, Sahún Roncero M, Colicchia V, Veschi V, et al. The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress. Cell Death Differ. 2016;23:197-206 pubmed publisher
  • western blot; human; fig 1
Hollingworth R, Skalka G, Stewart G, Hislop A, Blackbourn D, Grand R. Activation of DNA Damage Response Pathways during Lytic Replication of KSHV. Viruses. 2015;7:2908-27 pubmed publisher
  • western blot; human; fig 5
Xu Y, Wu X, Her C. hMSH5 Facilitates the Repair of Camptothecin-induced Double-strand Breaks through an Interaction with FANCJ. J Biol Chem. 2015;290:18545-58 pubmed publisher
  • western blot; human; fig 1
Kemp M, Lindsey Boltz L, Sancar A. UV Light Potentiates STING (Stimulator of Interferon Genes)-dependent Innate Immune Signaling through Deregulation of ULK1 (Unc51-like Kinase 1). J Biol Chem. 2015;290:12184-94 pubmed publisher
  • western blot; human
Wang B, Wu S, Tang S, Lai C, Ou C, Wu M, et al. Benzo[a]pyrene-induced cell cycle progression occurs via ERK-induced Chk1 pathway activation in human lung cancer cells. Mutat Res. 2015;773:1-8 pubmed publisher
  • western blot; human; fig 4
Zellweger R, Dalcher D, Mutreja K, Berti M, Schmid J, Herrador R, et al. Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells. J Cell Biol. 2015;208:563-79 pubmed publisher
  • reverse phase protein lysate microarray; human; 1:1000; fig 4
Thangavel S, Berti M, Levikova M, Pinto C, Gomathinayagam S, Vujanovic M, et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol. 2015;208:545-62 pubmed publisher
  • western blot; human
Nakahara T, Tanaka K, Ohno S, Egawa N, Yugawa T, Kiyono T. Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1. J Virol. 2015;89:5040-59 pubmed publisher
  • western blot; human; fig s7
Ekumi K, Paculova H, Lenasi T, Pospichalova V, Bösken C, Rybarikova J, et al. Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex. Nucleic Acids Res. 2015;43:2575-89 pubmed publisher
  • western blot; human; fig 4
Lindsey Boltz L, Kemp M, Capp C, Sancar A. RHINO forms a stoichiometric complex with the 9-1-1 checkpoint clamp and mediates ATR-Chk1 signaling. Cell Cycle. 2015;14:99-108 pubmed publisher
  • western blot; human; fig 1
Kehrli K, Sidorova J. Mitomycin C reduces abundance of replication forks but not rates of fork progression in primary and transformed human cells. Oncoscience. 2014;1:540-555 pubmed
  • western blot; human; 1:1000; fig s7
Ochi T, Blackford A, Coates J, Jhujh S, Mehmood S, Tamura N, et al. DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015;347:185-188 pubmed publisher
  • western blot; human; fig 3
Smith Roe S, Nakamura J, Holley D, Chastain P, Rosson G, Simpson D, et al. SWI/SNF complexes are required for full activation of the DNA-damage response. Oncotarget. 2015;6:732-45 pubmed
  • western blot; human; fig 6
Ray Chaudhuri A, Ahuja A, Herrador R, Lopes M. Poly(ADP-ribosyl) glycohydrolase prevents the accumulation of unusual replication structures during unperturbed S phase. Mol Cell Biol. 2015;35:856-65 pubmed publisher
  • western blot; human
Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T. Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication. Mol Cell Biol. 2015;35:699-715 pubmed publisher
  • western blot; African green monkey; fig 4a
Sowd G, Mody D, Eggold J, Cortez D, Friedman K, Fanning E. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS Pathog. 2014;10:e1004536 pubmed publisher
  • western blot; human
Kawasumi M, Bradner J, Tolliday N, Thibodeau R, Sloan H, Brummond K, et al. Identification of ATR-Chk1 pathway inhibitors that selectively target p53-deficient cells without directly suppressing ATR catalytic activity. Cancer Res. 2014;74:7534-45 pubmed publisher
  • western blot; human; fig 1b
Zhu M, Zhao H, Liao J, Xu X. HERC2/USP20 coordinates CHK1 activation by modulating CLASPIN stability. Nucleic Acids Res. 2014;42:13074-81 pubmed publisher
  • western blot; human; loading ...; fig 3e
Smith S, Petrova A, Madden M, Wang H, Pan Y, Warren M, et al. A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response. Nucleic Acids Res. 2014;42:11517-27 pubmed publisher
  • western blot; mouse; 1:1000; fig 4c
  • immunoprecipitation; human; 1:100
  • western blot; human; 1:1000; fig 4a
Zhang P, Wei Y, Wang L, Debeb B, Yuan Y, Zhang J, et al. ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol. 2014;16:864-75 pubmed publisher
  • western blot; human; 1:1000; fig s2
Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher
  • western blot; human
Yang X, Xu W, Hu Z, Zhang Y, Xu N. Chk1 is required for the metaphase-anaphase transition via regulating the expression and localization of Cdc20 and Mad2. Life Sci. 2014;106:12-8 pubmed publisher
  • western blot; chicken; fig 2
Frankenberger S, Davari K, Fischer Burkart S, Böttcher K, Tomi N, Zimber Strobl U, et al. Checkpoint kinase 1 negatively regulates somatic hypermutation. Nucleic Acids Res. 2014;42:3666-74 pubmed publisher
  • western blot; mouse; 1:2000; fig 2b
Lindsey Boltz L, Kemp M, Reardon J, DEROCCO V, Iyer R, Modrich P, et al. Coupling of human DNA excision repair and the DNA damage checkpoint in a defined in vitro system. J Biol Chem. 2014;289:5074-82 pubmed publisher
  • immunocytochemistry; mouse
  • western blot; mouse
Luebben S, Kawabata T, Akre M, Lee W, Johnson C, O Sullivan M, et al. Helq acts in parallel to Fancc to suppress replication-associated genome instability. Nucleic Acids Res. 2013;41:10283-97 pubmed publisher
  • western blot; human; fig 5d
Tomida J, Itaya A, Shigechi T, Unno J, Uchida E, Ikura M, et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res. 2013;41:6930-41 pubmed publisher
  • western blot; human
Hassan B, Lindsey Boltz L, Kemp M, Sancar A. Direct role for the replication protein treslin (Ticrr) in the ATR kinase-mediated checkpoint response. J Biol Chem. 2013;288:18903-10 pubmed publisher
  • western blot; human
  • western blot; chicken
Tuul M, Kitao H, Iimori M, Matsuoka K, Kiyonari S, Saeki H, et al. Rad9, Rad17, TopBP1 and claspin play essential roles in heat-induced activation of ATR kinase and heat tolerance. PLoS ONE. 2013;8:e55361 pubmed publisher
  • western blot; mouse; 1:200
Rass E, Chandramouly G, Zha S, Alt F, Xie A. Ataxia telangiectasia mutated (ATM) is dispensable for endonuclease I-SceI-induced homologous recombination in mouse embryonic stem cells. J Biol Chem. 2013;288:7086-95 pubmed publisher
  • western blot; human
Sidorova J, Kehrli K, Mao F, Monnat R. Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling. DNA Repair (Amst). 2013;12:128-39 pubmed publisher
  • western blot; human
Jullien L, Mestre M, Roux P, Gire V. Eroded human telomeres are more prone to remain uncapped and to trigger a G2 checkpoint response. Nucleic Acids Res. 2013;41:900-11 pubmed publisher
  • western blot; human
Sakasai R, Sakai A, Iimori M, Kiyonari S, Matsuoka K, Kakeji Y, et al. CtIP- and ATR-dependent FANCJ phosphorylation in response to DNA strand breaks mediated by DNA replication. Genes Cells. 2012;17:962-70 pubmed publisher
  • western blot; human
Cotta Ramusino C, McDonald E, Hurov K, Sowa M, Harper J, Elledge S. A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling. Science. 2011;332:1313-7 pubmed publisher
  • western blot; human
Day T, Palle K, Barkley L, Kakusho N, Zou Y, Tateishi S, et al. Phosphorylated Rad18 directs DNA polymerase η to sites of stalled replication. J Cell Biol. 2010;191:953-66 pubmed publisher
  • western blot; human
Mao F, Sidorova J, Lauper J, Emond M, Monnat R. The human WRN and BLM RecQ helicases differentially regulate cell proliferation and survival after chemotherapeutic DNA damage. Cancer Res. 2010;70:6548-55 pubmed publisher
  • western blot; mouse; loading ...; fig 4a
  • western blot; human; loading ...; fig 4c
Li D, Ohshiro K, Khan M, Kumar R. Requirement of MTA1 in ATR-mediated DNA damage checkpoint function. J Biol Chem. 2010;285:19802-12 pubmed publisher
  • immunocytochemistry; human; fig 4
Sakasai R, Teraoka H, Takagi M, Tibbetts R. Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison. J Biol Chem. 2010;285:15201-8 pubmed publisher
  • western blot; human; fig 2
Sakasai R, Teraoka H, Tibbetts R. Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin. DNA Repair (Amst). 2010;9:76-82 pubmed publisher
  • western blot; human
Solier S, Sordet O, Kohn K, Pommier Y. Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways. Mol Cell Biol. 2009;29:68-82 pubmed publisher
  • western blot; mouse; 1:1000
Li L, Dutra A, Pak E, Labrie J, Gerstein R, Pandolfi P, et al. EGFRvIII expression and PTEN loss synergistically induce chromosomal instability and glial tumors. Neuro Oncol. 2009;11:9-21 pubmed publisher
  • western blot; human
Sakasai R, Tibbetts R. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. J Biol Chem. 2008;283:13549-55 pubmed publisher
  • western blot; human
Adamson A, Beardsley D, Kim W, Gao Y, Baskaran R, Brown K. Methylator-induced, mismatch repair-dependent G2 arrest is activated through Chk1 and Chk2. Mol Biol Cell. 2005;16:1513-26 pubmed
Chakraborty A, Jenjaroenpun P, Li J, El Hilali S, McCulley A, Haarer B, et al. Replication Stress Induces Global Chromosome Breakage in the Fragile X Genome. Cell Rep. 2020;32:108179 pubmed publisher
Bai G, Kermi C, Stoy H, Schiltz C, Bacal J, Zaino A, et al. HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis. Mol Cell. 2020;78:1237-1251.e7 pubmed publisher
Chen J, Li P, Song L, Bai L, Huen M, Liu Y, et al. 53BP1 loss rescues embryonic lethality but not genomic instability of BRCA1 total knockout mice. Cell Death Differ. 2020;: pubmed publisher
Yang C, Kato H, Shindo M, Masai H. Cdc7 activates replication checkpoint by phosphorylating the Chk1-binding domain of Claspin in human cells. elife. 2019;8: pubmed publisher
Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, et al. FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops. Sci Rep. 2019;9:19110 pubmed publisher
Hustedt N, Alvarez Quilón A, McEwan A, Yuan J, Cho T, Koob L, et al. A consensus set of genetic vulnerabilities to ATR inhibition. Open Biol. 2019;9:190156 pubmed publisher
Fernández Antorán D, Piedrafita G, Murai K, Ong S, Herms A, Frezza C, et al. Outcompeting p53-Mutant Cells in the Normal Esophagus by Redox Manipulation. Cell Stem Cell. 2019;25:329-341.e6 pubmed publisher
Lyu X, Chastain M, Chai W. Genome-wide mapping and profiling of γH2AX binding hotspots in response to different replication stress inducers. BMC Genomics. 2019;20:579 pubmed publisher
Coulombe P, Nassar J, Peiffer I, Stanojcic S, Sterkers Y, Delamarre A, et al. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing. Nat Commun. 2019;10:2426 pubmed publisher
West K, Kelliher J, Xu Z, An L, Reed M, Eoff R, et al. LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation. Nucleic Acids Res. 2019;: pubmed publisher
Leimbacher P, Jones S, Shorrocks A, De Marco Zompit M, Day M, Blaauwendraad J, et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell. 2019;74:571-583.e8 pubmed publisher
Su H, Hu J, Huang L, Yang Y, Thenoz M, Kuchmiy A, et al. SHQ1 regulation of RNA splicing is required for T-lymphoblastic leukemia cell survival. Nat Commun. 2018;9:4281 pubmed publisher
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product information
Product Name :
Chk1 (G-4) Antibody
Gene Name :
CHEK1
Size :
200 µg/ml
Catalog Number :
sc-8408
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Chk1
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
FL (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA