This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Akt1/2/3 (H-136)
catalog :
sc-8312
conjugate :
nonconjugated
reactivity :
human, mouse, rat, zebrafish
application :
western blot
citations: 54
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 6a, 6b
Wang X, Zhao Y, Zhou D, Tian Y, Feng G, Lu Z. Gab2 deficiency suppresses high-fat diet-induced obesity by reducing adipose tissue inflammation and increasing brown adipose function in mice. Cell Death Dis. 2021;12:212 pubmed publisher
  • western blot; human; loading ...; fig 3h
Rigiracciolo D, Nohata N, Lappano R, Cirillo F, Talia M, Scordamaglia D, et al. IGF-1/IGF-1R/FAK/YAP Transduction Signaling Prompts Growth Effects in Triple-Negative Breast Cancer (TNBC) Cells. Cells. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 5c
Lin C, Lin W, Cho R, Yang C, Yeh Y, Hsiao L, et al. Induction of HO-1 by Mevastatin Mediated via a Nox/ROS-Dependent c-Src/PDGFRα/PI3K/Akt/Nrf2/ARE Cascade Suppresses TNF-α-Induced Lung Inflammation. J Clin Med. 2020;9: pubmed publisher
  • western blot; human; loading ...; fig 2
Xiao Z, Gaertner S, Morresi Hauf A, Genzel R, Duell T, Ullrich A, et al. Metformin Triggers Autophagy to Attenuate Drug-Induced Apoptosis in NSCLC Cells, with Minor Effects on Tumors of Diabetic Patients. Neoplasia. 2017;19:385-395 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 5a
Chang H, Chang Y, Lai S, Chen K, Wang K, Chiu T, et al. Naringenin inhibits migration of lung cancer cells via the inhibition of matrix metalloproteinases-2 and -9. Exp Ther Med. 2017;13:739-744 pubmed publisher
  • western blot; human; loading ...; fig 6a
Yang K, Chen Y, To K, Wang F, Li D, Chen L, et al. Alectinib (CH5424802) antagonizes ABCB1- and ABCG2-mediated multidrug resistance in vitro, in vivo and ex vivo. Exp Mol Med. 2017;49:e303 pubmed publisher
  • western blot; mouse; 1:1000; fig 13b
Koyama Y, Wang P, Liang S, Iwaisako K, Liu X, Xu J, et al. Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis. J Clin Invest. 2017;127:1254-1270 pubmed publisher
  • western blot; human; loading ...; fig 1b
Banerjee Mustafi S, Chakraborty P, Dwivedi S, Ding K, Moxley K, Mukherjee P, et al. BMI1, a new target of CK2?. Mol Cancer. 2017;16:56 pubmed publisher
  • western blot; human; loading ...; fig 5a
Kim H, Lee S, Kim C, Kim Y, Ju W, Kim S. Subcellular localization of FOXO3a as a potential biomarker of response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cancer cells. Oncotarget. 2017;8:6608-6622 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4a
Dragoj M, Milosević Z, Bankovic J, Tanic N, Pesic M, Stankovic T. Targeting CXCR4 and FAK reverses doxorubicin resistance and suppresses invasion in non-small cell lung carcinoma. Cell Oncol (Dordr). 2017;40:47-62 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 6a
Visuttijai K, Pettersson J, Mehrbani Azar Y, van den Bout I, Orndal C, Marcickiewicz J, et al. Lowered Expression of Tumor Suppressor Candidate MYO1C Stimulates Cell Proliferation, Suppresses Cell Adhesion and Activates AKT. PLoS ONE. 2016;11:e0164063 pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 8a
Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed
  • western blot; human; 1:1000; loading ...; fig 5a
Zeng Y, Yao X, Chen L, Yan Z, Liu J, Zhang Y, et al. Sphingosine-1-phosphate induced epithelial-mesenchymal transition of hepatocellular carcinoma via an MMP-7/ syndecan-1/TGF-β autocrine loop. Oncotarget. 2016;7:63324-63337 pubmed publisher
  • western blot; human; loading ...; fig 2b
Singh A, Fedele C, Lu H, Nevalainen M, Keen J, Languino L. Exosome-mediated Transfer of αvβ3 Integrin from Tumorigenic to Nontumorigenic Cells Promotes a Migratory Phenotype. Mol Cancer Res. 2016;14:1136-1146 pubmed
  • western blot; human; 1:2000; loading ...; fig 5d
Tisza M, Zhao W, Fuentes J, Prijic S, Chen X, Levental I, et al. Motility and stem cell properties induced by the epithelial-mesenchymal transition require destabilization of lipid rafts. Oncotarget. 2016;7:51553-51568 pubmed publisher
  • western blot; human; loading ...; fig 7
Rorsman C, Tsioumpekou M, Heldin C, Lennartsson J. The Ubiquitin Ligases c-Cbl and Cbl-b Negatively Regulate Platelet-derived Growth Factor (PDGF) BB-induced Chemotaxis by Affecting PDGF Receptor β (PDGFRβ) Internalization and Signaling. J Biol Chem. 2016;291:11608-18 pubmed publisher
  • western blot; zebrafish ; 1:1000; fig 2
Hirth S, Bühler A, Bührdel J, Rudeck S, Dahme T, Rottbauer W, et al. Paxillin and Focal Adhesion Kinase (FAK) Regulate Cardiac Contractility in the Zebrafish Heart. PLoS ONE. 2016;11:e0150323 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Chen C, Lee T, Kwok C, Hsu Y, Shih K, Lin Y, et al. Cannabinoid receptor type 1 mediates high-fat diet-induced insulin resistance by increasing forkhead box O1 activity in a mouse model of obesity. Int J Mol Med. 2016;37:743-54 pubmed publisher
  • western blot; mouse; loading ...; fig 7d
Chen Y, Zheng Y, You X, Yu M, Fu G, Su X, et al. Kras Is Critical for B Cell Lymphopoiesis. J Immunol. 2016;196:1678-85 pubmed publisher
  • western blot; rat; 1:500; loading ...
Romano López A, Méndez Díaz M, García F, Regalado Santiago C, Ruiz Contreras A, Prospero Garcia O. Maternal separation and early stress cause long-lasting effects on dopaminergic and endocannabinergic systems and alters dendritic morphology in the nucleus accumbens and frontal cortex in rats. Dev Neurobiol. 2016;76:819-31 pubmed publisher
  • western blot; human; fig 1
Gustavo Vazquez Jimenez J, Chavez Reyes J, Romero Garcia T, Zarain Herzberg A, Valdes Flores J, Manuel Galindo Rosales J, et al. Palmitic acid but not palmitoleic acid induces insulin resistance in a human endothelial cell line by decreasing SERCA pump expression. Cell Signal. 2016;28:53-9 pubmed publisher
  • western blot; human; fig 3
Mehner C, Oberg A, Kalli K, Nassar A, Hockla A, Pendlebury D, et al. Serine protease inhibitor Kazal type 1 (SPINK1) drives proliferation and anoikis resistance in a subset of ovarian cancers. Oncotarget. 2015;6:35737-54 pubmed publisher
  • western blot; mouse; 1:2000; fig 1b
  • western blot; human; 1:2000; fig 1c
Lee M, Jeong M, Lee H, Han H, Ko A, Hewitt S, et al. PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis. Nat Commun. 2015;6:7769 pubmed publisher
  • western blot; mouse; fig 6
Arruda A, Pers B, Parlakgül G, Güney E, Inouye K, Hotamisligil G. Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity. Nat Med. 2014;20:1427-35 pubmed publisher
Lee J, Lim Y, Kim J. UCH-L1 and UCH-L3 regulate the cancer stem cell-like properties through PI3 K/Akt signaling pathway in prostate cancer cells. Anim Cells Syst (Seoul). 2021;25:312-322 pubmed publisher
Cheng C, Liao W, Chen P, Yu J, Shiau H, Hsieh Y, et al. MiR-139 Modulates Cancer Stem Cell Function of Human Breast Cancer through Targeting CXCR4. Cancers (Basel). 2021;13: pubmed publisher
Sakai H, Shiina I, Shinomiya T, Nagahara Y. BRAP2 inhibits the Ras/Raf/MEK and PI3K/Akt pathways in leukemia cells, thereby inducing apoptosis and inhibiting cell growth. Exp Ther Med. 2021;21:463 pubmed publisher
Papke C, Smolen K, Swingle M, Cressey L, Heng R, Toporsian M, et al. A disorder-related variant (E420K) of a PP2A-regulatory subunit (PPP2R5D) causes constitutively active AKT-mTOR signaling and uncoordinated cell growth. J Biol Chem. 2021;296:100313 pubmed publisher
Garcia Puga M, Sáenz Antoñanzas A, Fernandez Torron R, Munain A, Matheu A. Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metformin. Aging (Albany NY). 2020;12:6260-6275 pubmed publisher
Nam K, Yi S, Nam G, Noh J, Park J, Lee M, et al. Identification of a novel S6K1 inhibitor, rosmarinic acid methyl ester, for treating cisplatin-resistant cervical cancer. BMC Cancer. 2019;19:773 pubmed publisher
Schreiber C, Saraswati S, Harkins S, Gruber A, Cremers N, Thiele W, et al. Loss of ASAP1 in mice impairs adipogenic and osteogenic differentiation of mesenchymal progenitor cells through dysregulation of FAK/Src and AKT signaling. PLoS Genet. 2019;15:e1008216 pubmed publisher
Sivaram N, McLaughlin P, Han H, Petrenko O, Jiang Y, Ballou L, et al. Tumor-intrinsic PIK3CA represses tumor immunogenecity in a model of pancreatic cancer. J Clin Invest. 2019;130: pubmed publisher
Hwang J, Kim A, Kim K, Il Park J, Oh H, Moon S, et al. TAZ couples Hippo/Wnt signalling and insulin sensitivity through Irs1 expression. Nat Commun. 2019;10:421 pubmed publisher
Fenizia C, Bottino C, Corbetta S, Fittipaldi R, Floris P, Gaudenzi G, et al. SMYD3 promotes the epithelial-mesenchymal transition in breast cancer. Nucleic Acids Res. 2019;47:1278-1293 pubmed publisher
Baek H, Kim S, Choi E, Kim J, Shin D, Park E, et al. Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1-associated Mammary Tumor Formation. Int J Biol Sci. 2018;14:1755-1768 pubmed publisher
Dai B, Li H, Fan J, Zhao Y, Yin Z, Nie X, et al. MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovasc Diabetol. 2018;17:123 pubmed publisher
García Gil A, Galán Enríquez C, Perez Lopez A, Nava P, Alpuche Aranda C, Ortiz Navarrete V. SopB activates the Akt-YAP pathway to promote Salmonella survival within B cells. Virulence. 2018;9:1390-1402 pubmed publisher
Cho R, Yang C, Tseng H, Hsiao L, Lin C, Yang C. Haem oxygenase-1 up-regulation by rosiglitazone via ROS-dependent Nrf2-antioxidant response elements axis or PPARγ attenuates LPS-mediated lung inflammation. Br J Pharmacol. 2018;175:3928-3946 pubmed publisher
Li X, Zhong L, Wang Z, Chen H, Liao D, Zhang R, et al. Phosphorylation of IRS4 by CK1γ2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway. Theranostics. 2018;8:3643-3653 pubmed publisher
Cho J, You Y, Yeom Y, Lee D, Kim B, Won M, et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis. 2018;9:587 pubmed publisher
Yu Y, Du H, Wei S, Feng L, Li J, Yao F, et al. Adipocyte-Derived Exosomal MiR-27a Induces Insulin Resistance in Skeletal Muscle Through Repression of PPARγ. Theranostics. 2018;8:2171-2188 pubmed publisher
Wang B, Luo Y, Zhou X, Li R. Trifluoperazine induces apoptosis through the upregulation of Bax/Bcl‑2 and downregulated phosphorylation of AKT in mesangial cells and improves renal function in lupus nephritis mice. Int J Mol Med. 2018;41:3278-3286 pubmed publisher
Tankiewicz Kwedlo A, Hermanowicz J, Domaniewski T, Pawlak K, Rusak M, Pryczynicz A, et al. Simultaneous use of erythropoietin and LFM-A13 as a new therapeutic approach for colorectal cancer. Br J Pharmacol. 2017;: pubmed publisher
Thaler S, Schmidt M, Roβwag S, Thiede G, Schad A, Sleeman J. Proteasome inhibitors prevent bi-directional HER2/estrogen-receptor cross-talk leading to cell death in endocrine and lapatinib-resistant HER2+/ER+ breast cancer cells. Oncotarget. 2017;8:72281-72301 pubmed publisher
Kong X, Kuilman T, Shahrabi A, Boshuizen J, Kemper K, Song J, et al. Cancer drug addiction is relayed by an ERK2-dependent phenotype switch. Nature. 2017;550:270-274 pubmed publisher
Skarra D, Hernandez Carretero A, Rivera A, Anvar A, Thackray V. Hyperandrogenemia Induced by Letrozole Treatment of Pubertal Female Mice Results in Hyperinsulinemia Prior to Weight Gain and Insulin Resistance. Endocrinology. 2017;158:2988-3003 pubmed publisher
Yang Z, Su Z, Dewitt J, Xie L, Chen Y, Li X, et al. Fluvastatin Prevents Lung Adenocarcinoma Bone Metastasis by Triggering Autophagy. EBioMedicine. 2017;19:49-59 pubmed publisher
Schmukler E, Wolfson E, Elazar Z, Kloog Y, Pinkas Kramarski R. Continuous treatment with FTS confers resistance to apoptosis and affects autophagy. PLoS ONE. 2017;12:e0171351 pubmed publisher
Fang Q, Guo S, Zhou H, Han R, Wu P, Han C. Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis. Sci Rep. 2017;7:41440 pubmed publisher
Wolfson E, Goldenberg M, Solomon S, Frishberg A, Pinkas Kramarski R. Nucleolin-binding by ErbB2 enhances tumorigenicity of ErbB2-positive breast cancer. Oncotarget. 2016;7:65320-65334 pubmed publisher
Song X, Ding Y, Liu G, Yang X, Zhao R, Zhang Y, et al. Cancer Cell-derived Exosomes Induce Mitogen-activated Protein Kinase-dependent Monocyte Survival by Transport of Functional Receptor Tyrosine Kinases. J Biol Chem. 2016;291:8453-64 pubmed publisher
Grassi D, Ghorbanpoor S, Acaz Fonseca E, Ruiz Palmero I, Garcia Segura L. The Selective Estrogen Receptor Modulator Raloxifene Regulates Arginine-Vasopressin Gene Expression in Human Female Neuroblastoma Cells Through G Protein-Coupled Estrogen Receptor and ERK Signaling. Endocrinology. 2015;156:3706-16 pubmed publisher
Khalil H, Peltzer N, Walicki J, Yang J, Dubuis G, Gardiol N, et al. Caspase-3 protects stressed organs against cell death. Mol Cell Biol. 2012;32:4523-33 pubmed publisher
Bulat N, Jaccard E, Peltzer N, Khalil H, Yang J, Dubuis G, et al. RasGAP-derived fragment N increases the resistance of beta cells towards apoptosis in NOD mice and delays the progression from mild to overt diabetes. PLoS ONE. 2011;6:e22609 pubmed publisher
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA