product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Dvl-3 (4D3) Antibody
catalog :
sc-8027
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4D3
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation
citations: 36
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; 1:1000; loading ...; fig 6f
Agajanian M, Walker M, Axtman A, Ruela de Sousa R, Serafin D, Rabinowitz A, et al. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Rep. 2019;26:79-93.e8 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 4d
  • western blot; human; loading ...; fig 4d
  • western blot; mouse; loading ...; fig 5c
Shami Shah A, Batrouni A, Kim D, Punyala A, Cao W, Han C, et al. PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling. Cell Rep. 2019;27:2157-2170.e8 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s2c
  • western blot; human; 1:200
Dass R, Sarshad A, Carson B, Feenstra J, Kaur A, Obrdlik A, et al. Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I. PLoS Genet. 2016;12:e1006217 pubmed publisher
  • western blot; rat; 1:1000; fig 3
Pan B, Huang X, Deng C. Chronic administration of aripiprazole activates GSK3β-dependent signalling pathways, and up-regulates GABAA receptor expression and CREB1 activity in rats. Sci Rep. 2016;6:30040 pubmed publisher
  • western blot; rat; 1:1000; fig 2
Pan B, Huang X, Deng C. Aripiprazole and Haloperidol Activate GSK3?-Dependent Signalling Pathway Differentially in Various Brain Regions of Rats. Int J Mol Sci. 2016;17:459 pubmed publisher
  • immunohistochemistry; mouse; fig 6
Liu C, Lin C, Gao C, May Simera H, Swaroop A, Li T. Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a. Biol Open. 2014;3:861-70 pubmed publisher
  • western blot; human; 1:500
Kilander M, Petersen J, Andressen K, Ganji R, Levy F, Schuster J, et al. Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6. FASEB J. 2014;28:2293-305 pubmed publisher
  • western blot; human
Kriz V, Pospichalova V, Masek J, Kilander M, Slavík J, Tanneberger K, et al. ?-arrestin promotes Wnt-induced low density lipoprotein receptor-related protein 6 (Lrp6) phosphorylation via increased membrane recruitment of Amer1 protein. J Biol Chem. 2014;289:1128-41 pubmed publisher
  • western blot; human; fig 6
Revet I, Huizenga G, Koster J, Volckmann R, van Sluis P, Versteeg R, et al. MSX1 induces the Wnt pathway antagonist genes DKK1, DKK2, DKK3, and SFRP1 in neuroblastoma cells, but does not block Wnt3 and Wnt5A signalling to DVL3. Cancer Lett. 2010;289:195-207 pubmed publisher
  • western blot; human
Bryja V, Schulte G, Rawal N, Grahn A, Arenas E. Wnt-5a induces Dishevelled phosphorylation and dopaminergic differentiation via a CK1-dependent mechanism. J Cell Sci. 2007;120:586-95 pubmed
Barbero G, Castro M, Villanueva M, Quezada M, Fernández N, Demorrow S, et al. An Autocrine Wnt5a Loop Promotes NF-κB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma. Cells. 2019;8: pubmed publisher
Harnoš J, Canizal M, Jurásek M, Kumar J, Holler C, Schambony A, et al. Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. Nat Commun. 2019;10:1804 pubmed publisher
Lo R, Leung C, Chan K, Ho D, Wong C, Lee T, et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/?-catenin pathway. Cell Death Differ. 2018;25:1426-1441 pubmed publisher
Paclíková P, Bernatik O, Radaszkiewicz T, Bryja V. The N-Terminal Part of the Dishevelled DEP Domain Is Required for Wnt/β-Catenin Signaling in Mammalian Cells. Mol Cell Biol. 2017;37: pubmed publisher
Halleskog C, Schulte G. Pertussis toxin-sensitive heterotrimeric G(?i/o) proteins mediate WNT/?-catenin and WNT/ERK1/2 signaling in mouse primary microglia stimulated with purified WNT-3A. Cell Signal. 2013;25:822-8 pubmed publisher
Cajanek L, Ganji R, Henriques Oliveira C, Theofilopoulos S, Konik P, Bryja V, et al. Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons. Mol Cell Biol. 2013;33:59-70 pubmed publisher
Wang Y, Wang H. Dvl3 translocates IPMK to the cell membrane in response to Wnt. Cell Signal. 2012;24:2389-95 pubmed publisher
Halleskog C, Dijksterhuis J, Kilander M, Becerril Ortega J, Villaescusa J, Lindgren E, et al. Heterotrimeric G protein-dependent WNT-5A signaling to ERK1/2 mediates distinct aspects of microglia proinflammatory transformation. J Neuroinflammation. 2012;9:111 pubmed
Bikkavilli R, Malbon C. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. J Cell Sci. 2012;125:2446-56 pubmed publisher
Yamagata K, Li X, Ikegaki S, Oneyama C, Okada M, Nishita M, et al. Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase (MMP-13) expression. J Biol Chem. 2012;287:1588-99 pubmed publisher
Bikkavilli R, Malbon C. Arginine methylation of G3BP1 in response to Wnt3a regulates ?-catenin mRNA. J Cell Sci. 2011;124:2310-20 pubmed publisher
Kilander M, Halleskog C, Schulte G. Recombinant WNTs differentially activate ?-catenin-dependent and -independent signalling in mouse microglia-like cells. Acta Physiol (Oxf). 2011;203:363-72 pubmed publisher
Liang J, Fu Y, Cruciat C, Jia S, Wang Y, Tong Z, et al. Transmembrane protein 198 promotes LRP6 phosphorylation and Wnt signaling activation. Mol Cell Biol. 2011;31:2577-90 pubmed publisher
Ren D, Minami Y, Nishita M. Critical role of Wnt5a-Ror2 signaling in motility and invasiveness of carcinoma cells following Snail-mediated epithelial-mesenchymal transition. Genes Cells. 2011;16:304-15 pubmed publisher
Bernatik O, Ganji R, Dijksterhuis J, Konik P, Cervenka I, Polonio T, et al. Sequential activation and inactivation of Dishevelled in the Wnt/beta-catenin pathway by casein kinases. J Biol Chem. 2011;286:10396-410 pubmed publisher
Nishita M, Itsukushima S, Nomachi A, Endo M, Wang Z, Inaba D, et al. Ror2/Frizzled complex mediates Wnt5a-induced AP-1 activation by regulating Dishevelled polymerization. Mol Cell Biol. 2010;30:3610-9 pubmed publisher
Sutton G, Madigan M, Roufas A, McAvoy J. Secreted frizzled-related protein 1 (SFRP1) is highly upregulated in keratoconus epithelium: a novel finding highlighting a new potential focus for keratoconus research and treatment. Clin Exp Ophthalmol. 2010;38:43-8 pubmed publisher
Witte F, Bernatik O, Kirchner K, Masek J, Mahl A, Krejci P, et al. Negative regulation of Wnt signaling mediated by CK1-phosphorylated Dishevelled via Ror2. FASEB J. 2010;24:2417-26 pubmed publisher
Chen M, Wang J, Lu J, Bond M, Ren X, Lyerly H, et al. The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling. Biochemistry. 2009;48:10267-74 pubmed publisher
Wolf V, Endo Y, Rubin J. Purification and Wnt-inhibitory activities of secreted frizzled-related proteins. Methods Mol Biol. 2008;468:31-44 pubmed publisher
Endo Y, Beauchamp E, Woods D, Taylor W, Toretsky J, Uren A, et al. Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism. Mol Cell Biol. 2008;28:2368-79 pubmed publisher
Zeng X, Huang H, Tamai K, Zhang X, Harada Y, Yokota C, et al. Initiation of Wnt signaling: control of Wnt coreceptor Lrp6 phosphorylation/activation via frizzled, dishevelled and axin functions. Development. 2008;135:367-75 pubmed
Bryja V, Cajanek L, Grahn A, Schulte G. Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation. Acta Physiol (Oxf). 2007;190:55-61 pubmed
Nishita M, Yoo S, Nomachi A, Kani S, Sougawa N, Ohta Y, et al. Filopodia formation mediated by receptor tyrosine kinase Ror2 is required for Wnt5a-induced cell migration. J Cell Biol. 2006;175:555-62 pubmed
Angers S, Thorpe C, Biechele T, Goldenberg S, Zheng N, MacCoss M, et al. The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation. Nat Cell Biol. 2006;8:348-57 pubmed
Endo Y, Wolf V, Muraiso K, Kamijo K, Soon L, Uren A, et al. Wnt-3a-dependent cell motility involves RhoA activation and is specifically regulated by dishevelled-2. J Biol Chem. 2005;280:777-86 pubmed
product information
Product Name :
Dvl-3 (4D3) Antibody
Gene Name :
DVL3
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-8027
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Dvl-3
reactive species  :
m, r, h
Application :
WB, IP
Epitope :
607-704 (m)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA