product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
p38alpha/beta (A-12) Antibody
catalog :
sc-7972
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A-12
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, other, western blot knockout validation
citations: 75
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 2
Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
  • western blot knockout validation; mouse; 1:2000; fig 1
Jamaladdin S, Kelly R, O Regan L, Dovey O, Hodson G, Millard C, et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A. 2014;111:9840-5 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 5m
Wu Y, Guo Q, Ju X, Hu Z, Xia L, Deng Y, et al. HNRNPH1-stabilized LINC00662 promotes ovarian cancer progression by activating the GRP78/p38 pathway. Oncogene. 2021;40:4770-4782 pubmed publisher
  • flow cytometry; human; loading ...; fig 6b
Sun R, Hedl M, Abraham C. TNFSF15 Promotes Antimicrobial Pathways in Human Macrophages and These Are Modulated by TNFSF15 Disease-Risk Variants. Cell Mol Gastroenterol Hepatol. 2021;11:249-272 pubmed publisher
  • western blot; human; 1:1000; fig 3f
Park M, Kim A, Manandhar S, Oh S, Jang G, Kang L, et al. CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity. elife. 2019;8: pubmed publisher
  • western blot; human; loading ...; fig 1b
Qiang L, Wang J, Zhang Y, Ge P, Chai Q, Li B, et al. Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation. Cell Mol Immunol. 2018;: pubmed publisher
  • western blot; human; loading ...; fig 5a
Arrizabalaga O, Moreno Cugnon L, Auzmendi Iriarte J, Aldaz P, Ibanez de Caceres I, Garros Regulez L, et al. High expression of MKP1/DUSP1 counteracts glioma stem cell activity and mediates HDAC inhibitor response. Oncogenesis. 2017;6:401 pubmed publisher
  • western blot; human; loading ...; fig 3e
Moua P, Checketts M, Xu L, Shu H, Reyland M, Cusick J. RELT family members activate p38 and induce apoptosis by a mechanism distinct from TNFR1. Biochem Biophys Res Commun. 2017;491:25-32 pubmed publisher
  • other; rat; loading ...; fig 1
Kocic G, Veljkovic A, Kocic H, Colic M, Mihajlović D, Tomovic K, et al. Depurinized milk downregulates rat thymus MyD88/Akt/p38 function, NF-κB-mediated inflammation, caspase-1 activity but not the endonuclease pathway: in vitro/in vivo study. Sci Rep. 2017;7:41971 pubmed publisher
  • western blot; human; fig 5
Matias A, Manieri T, Cerchiaro G. Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation. Oxid Med Cell Longev. 2016;2016:6724585 pubmed publisher
  • western blot; human; loading ...; fig s1c
Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
  • western blot; mouse; fig 5
Chhibber Goel J, Coleman Vaughan C, Agrawal V, Sawhney N, Hickey E, Powell J, et al. γ-Secretase Activity Is Required for Regulated Intramembrane Proteolysis of Tumor Necrosis Factor (TNF) Receptor 1 and TNF-mediated Pro-apoptotic Signaling. J Biol Chem. 2016;291:5971-85 pubmed publisher
  • western blot; human
Kim H, Lim J, Kim J, Kim Y, Park S, Sohn J. Panaxydol, a component of Panax ginseng, induces apoptosis in cancer cells through EGFR activation and ER stress and inhibits tumor growth in mouse models. Int J Cancer. 2016;138:1432-41 pubmed publisher
  • western blot; mouse; fig 5
Kratochvill F, Gratz N, Qualls J, Van De Velde L, Chi H, Kovarik P, et al. Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner. Cancer Res. 2015;75:3054-64 pubmed publisher
  • western blot; human
Arana M, Tocchetti G, Domizi P, Arias A, Rigalli J, Ruiz M, et al. Coordinated induction of GST and MRP2 by cAMP in Caco-2 cells: Role of protein kinase A signaling pathway and toxicological relevance. Toxicol Appl Pharmacol. 2015;287:178-90 pubmed publisher
  • western blot; human; 1:500
Daniele S, Da Pozzo E, Zappelli E, Martini C. Trazodone treatment protects neuronal-like cells from inflammatory insult by inhibiting NF-?B, p38 and JNK. Cell Signal. 2015;27:1609-29 pubmed publisher
  • chromatin immunoprecipitation; human
  • western blot; human
Yi P, Chew L, Zhang Z, Ren H, Wang F, Cong X, et al. KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation. Mol Biol Cell. 2015;26:29-42 pubmed publisher
  • western blot; mouse; fig s3
Xu R, Hu Q, Ma Q, Liu C, Wang G. The protease Omi regulates mitochondrial biogenesis through the GSK3β/PGC-1α pathway. Cell Death Dis. 2014;5:e1373 pubmed publisher
  • western blot; human
Kim T, Kim J, Kim Z, Huang R, Chae Y, Wang R. Khz-cp (crude polysaccharide extract obtained from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis by increasing intracellular calcium levels and activating P38 and NADPH oxidase-dependent generation of reactive ox. BMC Complement Altern Med. 2014;14:236 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 8a
Clauzure M, Valdivieso A, Massip Copiz M, Schulman G, Teiber M, Santa Coloma T. Disruption of interleukin-1? autocrine signaling rescues complex I activity and improves ROS levels in immortalized epithelial cells with impaired cystic fibrosis transmembrane conductance regulator (CFTR) function. PLoS ONE. 2014;9:e99257 pubmed publisher
  • western blot; mouse; 1:200
Gladding C, Fan J, Zhang L, Wang L, Xu J, Li E, et al. Alterations in STriatal-Enriched protein tyrosine Phosphatase expression, activation, and downstream signaling in early and late stages of the YAC128 Huntington's disease mouse model. J Neurochem. 2014;130:145-59 pubmed publisher
  • western blot; mouse
De Sousa Coelho A, Relat J, Hondares E, Pérez Martí A, Ribas F, Villarroya F, et al. FGF21 mediates the lipid metabolism response to amino acid starvation. J Lipid Res. 2013;54:1786-97 pubmed publisher
Lee S, Ha J, Park J, Kang E, Jeon S, Han S, et al. Antioxidant and Anti-Inflammatory Effects of Bischofia javanica (Blume) Leaf Methanol Extracts through the Regulation of Nrf2 and TAK1. Antioxidants (Basel). 2021;10: pubmed publisher
Venkataraman B, Almarzooqi S, Raj V, Alhassani A, Alhassani A, Ahmed K, et al. Thymoquinone, a Dietary Bioactive Compound, Exerts Anti-Inflammatory Effects in Colitis by Stimulating Expression of the Colonic Epithelial PPAR-γ Transcription Factor. Nutrients. 2021;13: pubmed publisher
Yin K, Lee J, Liu Z, Kim H, Martin D, Wu D, et al. Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production. Cell Death Differ. 2021;28:2421-2435 pubmed publisher
Al Khayal K, Vaali Mohammed M, Elwatidy M, Bin Traiki T, Al Obeed O, Azam M, et al. A novel coordination complex of platinum (PT) induces cell death in colorectal cancer by altering redox balance and modulating MAPK pathway. BMC Cancer. 2020;20:685 pubmed publisher
Garcia Puga M, Sáenz Antoñanzas A, Fernandez Torron R, Munain A, Matheu A. Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metformin. Aging (Albany NY). 2020;12:6260-6275 pubmed publisher
Wu P, Lin T, Hou C, Chang Q, Wen K, Lin C, et al. 1,2-Bis[(3-Methoxyphenyl)Methyl]Ethane-1,2-Dicarboxylic Acid Reduces UVB-Induced Photodamage In Vitro and In Vivo. Antioxidants (Basel). 2019;8: pubmed publisher
Fan W, Gao X, Rao X, Shi Y, Liu X, Wang F, et al. Hsp70 Interacts with Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 To Regulate p38MAPK Stability and Myoblast Differentiation during Skeletal Muscle Regeneration. Mol Cell Biol. 2018;38: pubmed publisher
Lee S, Park S, Zou Y, Seo S, Jun C, Lee W, et al. Real-Time Monitoring of Cancer Cells in Live Mouse Bone Marrow. Front Immunol. 2018;9:1681 pubmed publisher
Lei F, Jin L, Liu X, Lai F, Yan X, Farrelly M, et al. RIP1 protects melanoma cells from apoptosis induced by BRAF/MEK inhibitors. Cell Death Dis. 2018;9:679 pubmed publisher
Li X, Shang E, Dong Q, Li Y, Zhang J, Xu S, et al. Small molecules capable of activating DNA methylation-repressed genes targeted by the p38 mitogen-activated protein kinase pathway. J Biol Chem. 2018;293:7423-7436 pubmed publisher
Yan J, Hedl M, Abraham C. An inflammatory bowel disease-risk variant in INAVA decreases pattern recognition receptor-induced outcomes. J Clin Invest. 2017;127:2192-2205 pubmed publisher
Guerra M, Kalwat M, McGlynn K, Cobb M. Sucralose activates an ERK1/2-ribosomal protein S6 signaling axis. FEBS Open Bio. 2017;7:174-186 pubmed publisher
Narsale A, Puppa M, Hardee J, VanderVeen B, Enos R, Murphy E, et al. Short-term pyrrolidine dithiocarbamate administration attenuates cachexia-induced alterations to muscle and liver in ApcMin/+ mice. Oncotarget. 2016;7:59482-59502 pubmed publisher
Magi S, Saeki Y, Kasamatsu M, Tashiro E, Imoto M. Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells. PLoS ONE. 2014;9:e96776 pubmed publisher
Bogazzi F, Raggi F, Russo D, Bohlooly Y M, Sardella C, Urbani C, et al. Growth hormone is necessary for the p53-mediated, obesity-induced insulin resistance in male C57BL/6J x CBA mice. Endocrinology. 2013;154:4226-36 pubmed publisher
Jiang Q, Li F, Shi K, Wu P, An J, Yang Y, et al. ATF4 activation by the p38MAPK-eIF4E axis mediates apoptosis and autophagy induced by selenite in Jurkat cells. FEBS Lett. 2013;587:2420-9 pubmed publisher
Lin F, Luo J, Gao W, Wu J, Shao Z, Wang Z, et al. COX-2 promotes breast cancer cell radioresistance via p38/MAPK-mediated cellular anti-apoptosis and invasiveness. Tumour Biol. 2013;34:2817-26 pubmed publisher
Liu H, Qu X, Yuan F, Zhang M, Fang W. A RTK-based functional RNAi screen reveals determinants of PTX-3 expression. Int J Clin Exp Pathol. 2013;6:660-8 pubmed
Oaks A, Frankfurt M, Finkelstein D, Sidhu A. Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function. PLoS ONE. 2013;8:e60378 pubmed publisher
Xiao L, Haack K, Zucker I. Angiotensin II regulates ACE and ACE2 in neurons through p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 signaling. Am J Physiol Cell Physiol. 2013;304:C1073-9 pubmed publisher
Schuchman E, Ge Y, Lai A, Borisov Y, Faillace M, Eliyahu E, et al. Pentosan polysulfate: a novel therapy for the mucopolysaccharidoses. PLoS ONE. 2013;8:e54459 pubmed publisher
Lu Q, Wainwright M, Harris V, Aggarwal S, Hou Y, Rau T, et al. Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures. Free Radic Biol Med. 2012;53:1139-51 pubmed publisher
Fan J, Gladding C, Wang L, Zhang L, Kaufman A, Milnerwood A, et al. P38 MAPK is involved in enhanced NMDA receptor-dependent excitotoxicity in YAC transgenic mouse model of Huntington disease. Neurobiol Dis. 2012;45:999-1009 pubmed publisher
Smith J, Xu Z, Tian J, Palmer D, Ng P, Byrnes A. The role of endosomal escape and mitogen-activated protein kinases in adenoviral activation of the innate immune response. PLoS ONE. 2011;6:e26755 pubmed publisher
Wang W, Pan K, Chen Y, Huang C, Zhang X. The acetylation of transcription factor HBP1 by p300/CBP enhances p16INK4A expression. Nucleic Acids Res. 2012;40:981-95 pubmed publisher
Bhatt K, Sodhi A, Chakraborty R. Role of mitogen-activated protein kinases in peptidoglycan-induced expression of inducible nitric oxide synthase and nitric oxide in mouse peritoneal macrophages: extracellular signal-related kinase, a negative regulator. Clin Vaccine Immunol. 2011;18:994-1001 pubmed publisher
Pillai V, Sundaresan N, Samant S, Wolfgeher D, Trivedi C, Gupta M. Acetylation of a conserved lysine residue in the ATP binding pocket of p38 augments its kinase activity during hypertrophy of cardiomyocytes. Mol Cell Biol. 2011;31:2349-63 pubmed publisher
Kim J, Yu S, Oh H, Lee J, Kim Y, Sohn J. Panaxydol induces apoptosis through an increased intracellular calcium level, activation of JNK and p38 MAPK and NADPH oxidase-dependent generation of reactive oxygen species. Apoptosis. 2011;16:347-58 pubmed publisher
Cervenka I, Wolf J, Masek J, Krejci P, Wilcox W, Kozubik A, et al. Mitogen-activated protein kinases promote WNT/beta-catenin signaling via phosphorylation of LRP6. Mol Cell Biol. 2011;31:179-89 pubmed publisher
Li B, Li X, Li Y, Guo H, Sun S, He Q, et al. The effects of hepatitis C virus non-structural protein 3 on cell growth mediated by extracellular signal-related kinase cascades in human hepatocytes in vitro. Int J Mol Med. 2010;26:273-9 pubmed
Adhikary G, Chew Y, Reece E, Eckert R. PKC-delta and -eta, MEKK-1, MEK-6, MEK-3, and p38-delta are essential mediators of the response of normal human epidermal keratinocytes to differentiating agents. J Invest Dermatol. 2010;130:2017-30 pubmed publisher
Andújar I, Recio M, Bacelli T, Giner R, Rios J. Shikonin reduces oedema induced by phorbol ester by interfering with IkappaBalpha degradation thus inhibiting translocation of NF-kappaB to the nucleus. Br J Pharmacol. 2010;160:376-88 pubmed publisher
Hendriks B, Seidl K, Chabot J. Two additive mechanisms impair the differentiation of 'substrate-selective' p38 inhibitors from classical p38 inhibitors in vitro. BMC Syst Biol. 2010;4:23 pubmed publisher
Yen A, Yang J. Cdc20 proteolysis requires p38 MAPK signaling and Cdh1-independent APC/C ubiquitination during spindle assembly checkpoint activation by cadmium. J Cell Physiol. 2010;223:327-34 pubmed publisher
Lowe J, Cha H, Yang Q, Fornace A. Nuclear factor-kappaB (NF-kappaB) is a novel positive transcriptional regulator of the oncogenic Wip1 phosphatase. J Biol Chem. 2010;285:5249-57 pubmed publisher
Gonzalo S, Grasa L, Arruebo M, Plaza M, Murillo M. Inhibition of p38 MAPK improves intestinal disturbances and oxidative stress induced in a rabbit endotoxemia model. Neurogastroenterol Motil. 2010;22:564-72, e123 pubmed publisher
Cui T, Ichikawa T, Yang M, Dong X, Li J, Cui T. An emerging role of deubiquitinating enzyme cylindromatosis (CYLD) in the tubulointerstitial inflammation of IgA nephropathy. Biochem Biophys Res Commun. 2009;390:307-12 pubmed publisher
Blander J. Analysis of the TLR/NF-kappaB pathway in antigen-presenting cells in malignancies promoted by inflammation. Methods Mol Biol. 2009;512:99-117 pubmed publisher
Knirsh R, Ben Dror I, Spangler B, Matthews G, Kuphal S, Bosserhoff A, et al. Loss of E-cadherin-mediated cell-cell contacts activates a novel mechanism for up-regulation of the proto-oncogene c-Jun. Mol Biol Cell. 2009;20:2121-9 pubmed publisher
Ho T, Chen S, Yang Y, Lo T, Hsieh J, Cheng H, et al. Cytosolic phospholipase A2-{alpha} is an early apoptotic activator in PEDF-induced endothelial cell apoptosis. Am J Physiol Cell Physiol. 2009;296:C273-84 pubmed publisher
Li Y, Qian H, Li X, Wang H, Yu J, Liu Y, et al. Adenoviral-mediated gene transfer of Gadd45a results in suppression by inducing apoptosis and cell cycle arrest in pancreatic cancer cell. J Gene Med. 2009;11:3-13 pubmed publisher
Papachristou D, Papadakou E, Basdra E, Baltopoulos P, Panagiotopoulos E, Papavassiliou A. Involvement of the p38 MAPK-NF-kappaB signal transduction pathway and COX-2 in the pathobiology of meniscus degeneration in humans. Mol Med. 2008;14:160-6 pubmed publisher
Rampalli S, Li L, Mak E, Ge K, Brand M, Tapscott S, et al. p38 MAPK signaling regulates recruitment of Ash2L-containing methyltransferase complexes to specific genes during differentiation. Nat Struct Mol Biol. 2007;14:1150-6 pubmed
Kim D, Park S, Kwon S, Na J, Huh C, Park K. Additive effects of heat and p38 MAPK inhibitor treatment on melanin synthesis. Arch Pharm Res. 2007;30:581-6 pubmed
Park E, Kim S, Na J, Ryu H, Youn S, Kim D, et al. Glutathione prevented dopamine-induced apoptosis of melanocytes and its signaling. J Dermatol Sci. 2007;47:141-9 pubmed
Yu J, Qian H, Li Y, Wang Y, Zhang X, Liang X, et al. Therapeutic effect of arsenic trioxide (As2O3) on cervical cancer in vitro and in vivo through apoptosis induction. Cancer Biol Ther. 2007;6:580-6 pubmed
Jack G, Cabrera M, Manning M, Slaughter S, Potts M, Helm R. Activated stress response pathways within multicellular aggregates utilize an autocrine component. Cell Signal. 2007;19:772-81 pubmed
Sakamoto K, Kuribayashi F, Nakamura M, Takeshige K. Involvement of p38 MAP kinase in not only activation of the phagocyte NADPH oxidase induced by formyl-methionyl-leucyl-phenylalanine but also determination of the extent of the activity. J Biochem. 2006;140:739-45 pubmed
Russ D, Lovering R. Influence of activation frequency on cellular signalling pathways during fatiguing contractions in rat skeletal muscle. Exp Physiol. 2006;91:957-66 pubmed
Sun X, Li W, Li Q, Zhang M, Xian X, Qi J, et al. Limb ischemic preconditioning induces brain ischemic tolerance via p38 MAPK. Brain Res. 2006;1084:165-74 pubmed
Kim D, Jeon S, Jeong Y, Kim S, Kwon S, Park K. Hydrogen peroxide is a mediator of indole-3-acetic acid/horseradish peroxidase-induced apoptosis. FEBS Lett. 2006;580:1439-46 pubmed
Schomisch S, Murdock D, Hedayati N, Carino J, Lesnefsky E, Cmolik B. Cardioplegia prevents ischemia-induced transcriptional alterations of cytoprotective genes in rat hearts: a DNA microarray study. J Thorac Cardiovasc Surg. 2005;130:1151 pubmed
Kim S, Kim D, Kwon S, Park E, Huh C, Youn S, et al. Protective effects of EGCG on UVB-induced damage in living skin equivalents. Arch Pharm Res. 2005;28:784-90 pubmed
product information
Product Name :
p38alpha/beta (A-12) Antibody
Gene Name :
MAPK14, MAPK11
Size :
200 µg/ml
Catalog Number :
sc-7972
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
p38alpha/beta
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
213-360 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA