product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Smad4 (B-8) Antibody
catalog :
sc-7966
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B-8
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, reverse phase protein lysate microarray, proximity ligation assay, other, western blot knockout validation
citations: 165
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; human; 1:1000; loading ...; fig 1b
Gori I, George R, Purkiss A, Strohbuecker S, Randall R, Ogrodowicz R, et al. Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-β responses through SKI stabilization. elife. 2021;10: pubmed publisher
  • western blot knockout validation; mouse; fig 1
Cao J, Zhang X, Wang Q, Qiu G, Hou C, Wang J, et al. Smad4 represses the generation of memory-precursor effector T cells but is required for the differentiation of central memory T cells. Cell Death Dis. 2015;6:e1984 pubmed publisher
  • western blot knockout validation; human; fig 2
Drost J, van Jaarsveld R, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, et al. Sequential cancer mutations in cultured human intestinal stem cells. Nature. 2015;521:43-7 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
  • immunocytochemistry; mouse; fig 1d
  • western blot; mouse; fig 1e
Eritja N, Navaridas R, Ruiz Mitjana A, Vidal Sabanés M, Egea J, Encinas M, et al. Endometrial PTEN Deficiency Leads to SMAD2/3 Nuclear Translocation. Cancers (Basel). 2021;13: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1d
Tan X, Tong L, Li L, Xu J, Xie S, Ji L, et al. Loss of Smad4 promotes aggressive lung cancer metastasis by de-repression of PAK3 via miRNA regulation. Nat Commun. 2021;12:4853 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 6b
Niu B, Liu J, Lv B, Lin J, Li X, Wu C, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12:2809 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4e
Beel S, Kolloch L, Apken L, Jürgens L, Bolle A, Sudhof N, et al. κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis. Nat Commun. 2020;11:3409 pubmed publisher
  • proximity ligation assay; human; 1:200; loading ...; fig 3e
Gibbs Z, Reza L, Cheng C, Westcott J, McGlynn K, Whitehurst A. The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer. elife. 2020;9: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3e
Matsumoto S, Yamamichi T, Shinzawa K, Kasahara Y, Nojima S, Kodama T, et al. GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling. Nat Commun. 2019;10:3882 pubmed publisher
  • western blot; human; loading ...; fig 4c
Jin H, Xue L, Mo L, Zhang D, Guo X, Xu J, et al. Downregulation of miR-200c stabilizes XIAP mRNA and contributes to invasion and lung metastasis of bladder cancer. Cell Adh Migr. 2019;13:236-248 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 3b
Tsai C, Tsai C, Yi J, Kao H, Huang Y, Wang C, et al. Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells. Sci Rep. 2019;9:5197 pubmed publisher
  • western blot; mouse; loading ...; fig 5b
Biffi G, Oni T, Spielman B, Hao Y, Elyada E, Park Y, et al. IL1-Induced JAK/STAT Signaling Is Antagonized by TGFβ to Shape CAF Heterogeneity in Pancreatic Ductal Adenocarcinoma. Cancer Discov. 2019;9:282-301 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50
Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s3a
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
  • western blot; human; loading ...; fig 4a
Hiramoto H, Muramatsu T, Ichikawa D, Tanimoto K, Yasukawa S, Otsuji E, et al. miR-509-5p and miR-1243 increase the sensitivity to gemcitabine by inhibiting epithelial-mesenchymal transition in pancreatic cancer. Sci Rep. 2017;7:4002 pubmed publisher
  • western blot; mouse; fig s16
Wassermann Dozorets R, Rubinstein M. C/EBPβ LIP augments cell death by inducing osteoglycin. Cell Death Dis. 2017;8:e2733 pubmed publisher
  • reverse phase protein lysate microarray; human; loading ...; fig st6
Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  • western blot; human; loading ...; fig s1b
Fumagalli A, Drost J, Suijkerbuijk S, van Boxtel R, de Ligt J, Offerhaus G, et al. Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids. Proc Natl Acad Sci U S A. 2017;114:E2357-E2364 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 1a
Whitaker A, Berthet E, Cantu A, Laird D, Alliston T. Smad4 regulates growth plate matrix production and chondrocyte polarity. Biol Open. 2017;6:358-364 pubmed publisher
  • reverse phase protein lysate microarray; human; loading ...; fig 3a
. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
  • immunohistochemistry; human; loading ...; fig s1h
Dey P, Baddour J, Muller F, Wu C, Wang H, Liao W, et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature. 2017;542:119-123 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Kocsis T, Trencsenyi G, Szabó K, Baán J, Müller G, Mendler L, et al. Myostatin propeptide mutation of the hypermuscular Compact mice decreases the formation of myostatin and improves insulin sensitivity. Am J Physiol Endocrinol Metab. 2017;312:E150-E160 pubmed publisher
  • flow cytometry; mouse; fig 1a
Paris N, Soroka A, Klose A, Liu W, Chakkalakal J. Smad4 restricts differentiation to promote expansion of satellite cell derived progenitors during skeletal muscle regeneration. elife. 2016;5: pubmed publisher
  • western blot; human; loading ...; fig 7e
Chan D, Hui W, Wang J, Yung M, Hui L, Qin Y, et al. DLX1 acts as a crucial target of FOXM1 to promote ovarian cancer aggressiveness by enhancing TGF-β/SMAD4 signaling. Oncogene. 2017;36:1404-1416 pubmed publisher
  • immunohistochemistry; human; 1:1000; loading ...; fig 4a
  • western blot; human; 1:200; loading ...; fig 3a
Kim D, Chang M, Yoon C, Middeldorp J, Martinez O, Byeon S, et al. Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells. Oncotarget. 2016;7:82213-82227 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:400; fig s1
Fessler E, Drost J, van Hooff S, Linnekamp J, Wang X, Jansen M, et al. TGFβ signaling directs serrated adenomas to the mesenchymal colorectal cancer subtype. EMBO Mol Med. 2016;8:745-60 pubmed publisher
  • western blot; human; 1:1000; fig 3
Watanabe Y, Papoutsoglou P, Maturi V, Tsubakihara Y, Hottiger M, Heldin C, et al. Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation. J Biol Chem. 2016;291:12706-23 pubmed publisher
  • western blot; human; fig 2
Liu L, Liu X, Ren X, Tian Y, Chen Z, Xu X, et al. Smad2 and Smad3 have differential sensitivity in relaying TGFβ signaling and inversely regulate early lineage specification. Sci Rep. 2016;6:21602 pubmed publisher
  • immunocytochemistry; human; fig 5
  • western blot; human; fig 4
Braun D, Sadowski C, Kohl S, Lovric S, Astrinidis S, Pabst W, et al. Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome. Nat Genet. 2016;48:457-65 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
Bacallao K, Plaza Parrochia F, Cerda A, Gabler F, Romero C, Vantman D, et al. Levels of Regulatory Proteins Associated With Cell Proliferation in Endometria From Untreated Patients Having Polycystic Ovarian Syndrome With and Without Endometrial Hyperplasia. Reprod Sci. 2016;23:211-8 pubmed publisher
  • proximity ligation assay; mouse; fig s12
Yoon J, Sudo K, Kuroda M, Kato M, Lee I, Han J, et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun. 2015;6:7600 pubmed publisher
  • immunohistochemistry; human; 1:250; loading ...; fig 1e
Kassem L, Deygas M, Fattet L, Lopez J, Goulvent T, Lavergne E, et al. TIF1γ interferes with TGFβ1/SMAD4 signaling to promote poor outcome in operable breast cancer patients. BMC Cancer. 2015;15:453 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
Bian C, Li Z, Xu Y, Wang J, Xu L, Shen H. Clinical outcome and expression of mutant P53, P16, and Smad4 in lung adenocarcinoma: a prospective study. World J Surg Oncol. 2015;13:128 pubmed publisher
  • western blot; human
Maity G, De A, Das A, Banerjee S, Sarkar S, Banerjee S. Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition. Lab Invest. 2015;95:702-17 pubmed publisher
  • western blot; human; fig s5b
Li Y, Drabsch Y, Pujuguet P, Ren J, van Laar T, Zhang L, et al. Genetic depletion and pharmacological targeting of αv integrin in breast cancer cells impairs metastasis in zebrafish and mouse xenograft models. Breast Cancer Res. 2015;17:28 pubmed publisher
  • western blot; human
Krzeminski P, Sarasquete M, Misiewicz Krzeminska I, Corral R, Corchete L, Martín A, et al. Insights into epigenetic regulation of microRNA-155 expression in multiple myeloma. Biochim Biophys Acta. 2015;1849:353-66 pubmed publisher
  • immunohistochemistry; human; 1:400
Voorneveld P, Kodach L, Jacobs R, van Noesel C, Peppelenbosch M, Korkmaz K, et al. The BMP pathway either enhances or inhibits the Wnt pathway depending on the SMAD4 and p53 status in CRC. Br J Cancer. 2015;112:122-30 pubmed publisher
  • chromatin immunoprecipitation; mouse; 2.0 ug
Strand D, Liang Y, Yang F, Barron D, Ressler S, Schauer I, et al. TGF-β induction of FGF-2 expression in stromal cells requires integrated smad3 and MAPK pathways. Am J Clin Exp Urol. 2014;2:239-48 pubmed
  • western blot; mouse; 1:1000; fig 1c
Feuerborn A, Mathow D, Srivastava P, Gretz N, Grone H. Basonuclin-1 modulates epithelial plasticity and TGF-?1-induced loss of epithelial cell integrity. Oncogene. 2015;34:1185-95 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200
Zhang X, Liu X, Joseph L, Zhao L, Hart J, Xiao S. Pancreatic ductal adenocarcinoma with autoimmune pancreatitis-like histologic and immunohistochemical features. Hum Pathol. 2014;45:621-7 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X, et al. Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-? signaling. J Biol Chem. 2014;289:2072-83 pubmed publisher
  • western blot; rat
Giampieri S, Manning C, Hooper S, Jones L, Hill C, Sahai E. Localized and reversible TGFbeta signalling switches breast cancer cells from cohesive to single cell motility. Nat Cell Biol. 2009;11:1287-96 pubmed publisher
  • western blot; human
Sergeeva A, Kolonin M, Molldrem J, Pasqualini R, Arap W. Display technologies: application for the discovery of drug and gene delivery agents. Adv Drug Deliv Rev. 2006;58:1622-54 pubmed
Kim J, Park J, Jeon E, Leem J, Park K. Melatonin Prevents Transforming Growth Factor-β1-Stimulated Transdifferentiation of Renal Interstitial Fibroblasts to Myofibroblasts by Suppressing Reactive Oxygen Species-Dependent Mechanisms. Antioxidants (Basel). 2020;9: pubmed publisher
Chen L, Toke N, Luo S, Vasoya R, Fullem R, Parthasarathy A, et al. A reinforcing HNF4-SMAD4 feed-forward module stabilizes enterocyte identity. Nat Genet. 2019;51:777-785 pubmed publisher
Fenizia C, Bottino C, Corbetta S, Fittipaldi R, Floris P, Gaudenzi G, et al. SMYD3 promotes the epithelial-mesenchymal transition in breast cancer. Nucleic Acids Res. 2019;47:1278-1293 pubmed publisher
Roh V, Abramowski P, Hiou Feige A, Cornils K, Rivals J, Zougman A, et al. Cellular Barcoding Identifies Clonal Substitution as a Hallmark of Local Recurrence in a Surgical Model of Head and Neck Squamous Cell Carcinoma. Cell Rep. 2018;25:2208-2222.e7 pubmed publisher
Zöller T, Schneider A, Kleimeyer C, Masuda T, Potru P, Pfeifer D, et al. Silencing of TGFβ signalling in microglia results in impaired homeostasis. Nat Commun. 2018;9:4011 pubmed publisher
Perekatt A, Shah P, Cheung S, Jariwala N, Wu A, Gandhi V, et al. SMAD4 Suppresses WNT-Driven Dedifferentiation and Oncogenesis in the Differentiated Gut Epithelium. Cancer Res. 2018;78:4878-4890 pubmed publisher
Yu S, Li L, Tian W, Nie D, Mu W, Qiu F, et al. PEP06 polypeptide 30 exerts antitumour effect in colorectal carcinoma via inhibiting epithelial-mesenchymal transition. Br J Pharmacol. 2018;175:3111-3130 pubmed publisher
Albers R, Selesniemi K, Natale D, Brown T. TGF-β induces Smad2 Phosphorylation, ARE Induction, and Trophoblast Differentiation. Int J Stem Cells. 2018;11:111-120 pubmed publisher
Vion A, Alt S, Klaus Bergmann A, Szymborska A, Zheng T, Perovic T, et al. Primary cilia sensitize endothelial cells to BMP and prevent excessive vascular regression. J Cell Biol. 2018;217:1651-1665 pubmed publisher
Sundqvist A, Morikawa M, Ren J, Vasilaki E, Kawasaki N, Kobayashi M, et al. JUNB governs a feed-forward network of TGF? signaling that aggravates breast cancer invasion. Nucleic Acids Res. 2017;: pubmed publisher
Ojiaku C, Cao G, Zhu W, Yoo E, Shumyatcher M, Himes B, et al. TGF-β1 Evokes Human Airway Smooth Muscle Cell Shortening and Hyperresponsiveness via Smad3. Am J Respir Cell Mol Biol. 2017;: pubmed publisher
Woo T, Yoon M, Hong S, Choi J, Ha N, Sun H, et al. Anti-cancer effect of novel PAK1 inhibitor via induction of PUMA-mediated cell death and p21-mediated cell cycle arrest. Oncotarget. 2017;8:23690-23701 pubmed publisher
Liu T, Zhao M, Liu J, He Z, Zhang Y, You H, et al. Tumor suppressor bromodomain-containing protein 7 cooperates with Smads to promote transforming growth factor-β responses. Oncogene. 2017;36:362-372 pubmed publisher
Kang Y, Roife D, Lee Y, Lv H, Suzuki R, Ling J, et al. Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res. 2016;22:4934-4946 pubmed
Li N, Zhang M, Drummen G, Zhao Y, Tan Y, Luo S, et al. Sika Deer Antler Collagen Type I-Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway. Evid Based Complement Alternat Med. 2016;2016:2109204 pubmed publisher
Lima C, Geraldo M, Fuziwara C, Kimura E, Santos M. MiRNA-146b-5p upregulates migration and invasion of different Papillary Thyroid Carcinoma cells. BMC Cancer. 2016;16:108 pubmed publisher
Park J, Park J, Park D, Kim D, Kim H. Stem Cells Antigen-1 Enriches for a Cancer Stem Cell-Like Subpopulation in Mouse Gastric Cancer. Stem Cells. 2016;34:1177-87 pubmed publisher
Park C, Skarra D, Rivera A, Arriola D, Thackray V. Constitutively active FOXO1 diminishes activin induction of Fshb transcription in immortalized gonadotropes. PLoS ONE. 2014;9:e113839 pubmed publisher
Kang Y, Ling J, Suzuki R, Roife D, Chopin Laly X, Truty M, et al. SMAD4 regulates cell motility through transcription of N-cadherin in human pancreatic ductal epithelium. PLoS ONE. 2014;9:e107948 pubmed publisher
Kowno M, Watanabe Susaki K, Ishimine H, Komazaki S, Enomoto K, Seki Y, et al. Prohibitin 2 regulates the proliferation and lineage-specific differentiation of mouse embryonic stem cells in mitochondria. PLoS ONE. 2014;9:e81552 pubmed publisher
Zhang J, Zhang Z, Zhang D, Zhu J, Zhang T, Wang C. microRNA 126 inhibits the transition of endothelial progenitor cells to mesenchymal cells via the PIK3R2-PI3K/Akt signalling pathway. PLoS ONE. 2013;8:e83294 pubmed publisher
Liu N, Xi Y, Callaghan M, Fribley A, Moore Smith L, Zimmerman J, et al. SMAD4 is a potential prognostic marker in human breast carcinomas. Tumour Biol. 2014;35:641-50 pubmed publisher
Zong J, Salim M, Zhou H, Bian Z, Dai J, Yuan Y, et al. NOD2 deletion promotes cardiac hypertrophy and fibrosis induced by pressure overload. Lab Invest. 2013;93:1128-36 pubmed publisher
Wang X, Liao P, Fan X, Wan Y, Wang Y, Li Y, et al. CXXC5 Associates with Smads to Mediate TNF-? Induced Apoptosis. Curr Mol Med. 2013;13:1385-96 pubmed
Gao Y, Lui W. Transforming growth factor-?1 (TGF-?1) regulates cell junction restructuring via Smad-mediated repression and clathrin-mediated endocytosis of nectin-like molecule 2 (Necl-2). PLoS ONE. 2013;8:e64316 pubmed publisher
Xia P, Gou W, Zhao S, Zheng H. Crizotinib may be used in Lewis lung carcinoma: a novel use for crizotinib. Oncol Rep. 2013;30:139-48 pubmed publisher
Kelly C, Thymiakou E, Dixon J, Tanaka S, Godwin J, Episkopou V. Rnf165/Ark2C enhances BMP-Smad signaling to mediate motor axon extension. PLoS Biol. 2013;11:e1001538 pubmed publisher
Zhao W, Wang K. NGX6 expression improves the sensitivity of tamoxifen-resistant MCF-7 cells through modulation of the Smad signaling pathway. Int J Oncol. 2013;42:2060-8 pubmed publisher
Su H, Weng C, Hsiao P, Chen L, Kuo T, Chen Y, et al. Stem cell marker nestin is critical for TGF-?1-mediated tumor progression in pancreatic cancer. Mol Cancer Res. 2013;11:768-79 pubmed publisher
Li X, Xu Y, Chen Y, Chen S, Jia X, Sun T, et al. SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/?-catenin signal network. Cancer Lett. 2013;336:379-89 pubmed publisher
Waldmeier L, Meyer Schaller N, Diepenbruck M, Christofori G. Py2T murine breast cancer cells, a versatile model of TGF?-induced EMT in vitro and in vivo. PLoS ONE. 2012;7:e48651 pubmed publisher
de Kruijf E, Dekker T, Hawinkels L, Putter H, Smit V, Kroep J, et al. The prognostic role of TGF-? signaling pathway in breast cancer patients. Ann Oncol. 2013;24:384-90 pubmed publisher
Lovric V, Chen D, Yu Y, Oliver R, Genin F, Walsh W. Effects of demineralized bone matrix on tendon-bone healing in an intra-articular rodent model. Am J Sports Med. 2012;40:2365-74 pubmed
Xu Y, Man X, Lv Z, Li D, Sun Z, Chen H, et al. Loss of heterozygosity at chromosomes 1p35-pter, 4q, and 18q and protein expression differences between adenocarcinomas of the distal stomach and gastric cardia. Hum Pathol. 2012;43:2308-17 pubmed publisher
Camilo V, Barros R, Sousa S, Magalhães A, Lopes T, Mário Santos A, et al. Helicobacter pylori and the BMP pathway regulate CDX2 and SOX2 expression in gastric cells. Carcinogenesis. 2012;33:1985-92 pubmed publisher
Guo W, Dong Z, Guo Y, Kuang G, Yang Z, Shan B. Concordant repression and aberrant methylation of transforming growth factor-beta signaling pathway genes occurs early in gastric cardia adenocarcinoma. Mol Biol Rep. 2012;39:9453-62 pubmed publisher
Perez Mancera P, Rust A, van der Weyden L, Kristiansen G, Li A, Sarver A, et al. The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma. Nature. 2012;486:266-70 pubmed publisher
Daroqui M, Vazquez P, Bal de Kier Joffe E, Bakin A, Puricelli L. TGF-? autocrine pathway and MAPK signaling promote cell invasiveness and in vivo mammary adenocarcinoma tumor progression. Oncol Rep. 2012;28:567-75 pubmed publisher
Kopf J, Petersen A, Duda G, Knaus P. BMP2 and mechanical loading cooperatively regulate immediate early signalling events in the BMP pathway. BMC Biol. 2012;10:37 pubmed publisher
Zieba A, Pardali K, Söderberg O, Lindbom L, Nyström E, Moustakas A, et al. Intercellular variation in signaling through the TGF-? pathway and its relation to cell density and cell cycle phase. Mol Cell Proteomics. 2012;11:M111.013482 pubmed publisher
Ueberham U, Hilbrich I, Ueberham E, Rohn S, Glöckner P, Dietrich K, et al. Transcriptional control of cell cycle-dependent kinase 4 by Smad proteins--implications for Alzheimer's disease. Neurobiol Aging. 2012;33:2827-40 pubmed publisher
Ottenhof N, Morsink F, Ten Kate F, Van Noorden C, Offerhaus G. Multivariate analysis of immunohistochemical evaluation of protein expression in pancreatic ductal adenocarcinoma reveals prognostic significance for persistent Smad4 expression only. Cell Oncol (Dordr). 2012;35:119-26 pubmed publisher
Lampropoulos P, Zizi Sermpetzoglou A, Rizos S, Kostakis A, Nikiteas N, Papavassiliou A. Prognostic significance of transforming growth factor beta (TGF-?) signaling axis molecules and E-cadherin in colorectal cancer. Tumour Biol. 2012;33:1005-14 pubmed publisher
Chiu C, Kuo K, Kuo T, Lee K, Cheng K. The activation of MEK/ERK signaling pathway by bone morphogenetic protein 4 to increase hepatocellular carcinoma cell proliferation and migration. Mol Cancer Res. 2012;10:415-27 pubmed publisher
García Solano J, Conesa Zamora P, Trujillo Santos J, Torres Moreno D, Mäkinen M, Pérez Guillermo M. Immunohistochemical expression profile of ?-catenin, E-cadherin, P-cadherin, laminin-5?2 chain, and SMAD4 in colorectal serrated adenocarcinoma. Hum Pathol. 2012;43:1094-102 pubmed publisher
Jain S, Singhal S, Francis F, Hajdu C, Wang J, Suriawinata A, et al. Association of overexpression of TIF1? with colorectal carcinogenesis and advanced colorectal adenocarcinoma. World J Gastroenterol. 2011;17:3994-4000 pubmed publisher
Mohri D, Asaoka Y, Ijichi H, Miyabayashi K, Kudo Y, Seto M, et al. Different subtypes of intraductal papillary mucinous neoplasm in the pancreas have distinct pathways to pancreatic cancer progression. J Gastroenterol. 2012;47:203-13 pubmed publisher
Ying H, Elpek K, Vinjamoori A, Zimmerman S, Chu G, Yan H, et al. PTEN is a major tumor suppressor in pancreatic ductal adenocarcinoma and regulates an NF-?B-cytokine network. Cancer Discov. 2011;1:158-69 pubmed publisher
Geraldo M, Yamashita A, Kimura E. MicroRNA miR-146b-5p regulates signal transduction of TGF-? by repressing SMAD4 in thyroid cancer. Oncogene. 2012;31:1910-22 pubmed publisher
He S, Zhao Z, Wang Y, Zhao J, Wang L, Hou F, et al. Reduced expression of SMAD4 in gliomas correlates with progression and survival of patients. J Exp Clin Cancer Res. 2011;30:70 pubmed publisher
Pérez Torras S, Vidal Pla A, Miquel R, Almendro V, Fernandez Cruz L, Navarro S, et al. Characterization of human pancreatic orthotopic tumor xenografts suitable for drug screening. Cell Oncol (Dordr). 2011;34:511-21 pubmed publisher
Quere R, Karlsson G, Hertwig F, Rissler M, Lindqvist B, Fioretos T, et al. Smad4 binds Hoxa9 in the cytoplasm and protects primitive hematopoietic cells against nuclear activation by Hoxa9 and leukemia transformation. Blood. 2011;117:5918-30 pubmed publisher
Zhang L, Zhou F, van Laar T, Zhang J, van Dam H, Ten Dijke P. Fas-associated factor 1 antagonizes Wnt signaling by promoting ?-catenin degradation. Mol Biol Cell. 2011;22:1617-24 pubmed publisher
Thymiakou E, Episkopou V. Detection of signaling effector-complexes downstream of bmp4 using PLA, a proximity ligation assay. J Vis Exp. 2011;: pubmed publisher
Leeuwis J, Nguyen T, Chuva de Sousa Lopes S, van der Giezen D, van der Ven K, Rouw P, et al. Direct visualization of Smad1/5/8-mediated transcriptional activity identifies podocytes and collecting ducts as major targets of BMP signalling in healthy and diseased kidneys. J Pathol. 2011;224:121-32 pubmed publisher
Ding Z, Wu C, Chu G, Xiao Y, Ho D, Zhang J, et al. SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression. Nature. 2011;470:269-73 pubmed publisher
Moutasim K, Jenei V, Sapienza K, Marsh D, Weinreb P, Violette S, et al. Betel-derived alkaloid up-regulates keratinocyte alphavbeta6 integrin expression and promotes oral submucous fibrosis. J Pathol. 2011;223:366-77 pubmed publisher
Fukunishi N, Katoh I, Tomimori Y, Tsukinoki K, Hata R, Nakao A, et al. Induction of ΔNp63 by the newly identified keratinocyte-specific transforming growth factor β Signaling Pathway with Smad2 and IκB Kinase α in squamous cell carcinoma. Neoplasia. 2010;12:969-79 pubmed
Proietti S, Cucina A, D Anselmi F, Dinicola S, Pasqualato A, Lisi E, et al. Melatonin and vitamin D3 synergistically down-regulate Akt and MDM2 leading to TGF?-1-dependent growth inhibition of breast cancer cells. J Pineal Res. 2011;50:150-8 pubmed publisher
Brandl M, Seidler B, Haller F, Adamski J, Schmid R, Saur D, et al. IKK(?) controls canonical TGF(ß)-SMAD signaling to regulate genes expressing SNAIL and SLUG during EMT in panc1 cells. J Cell Sci. 2010;123:4231-9 pubmed publisher
Ali N, McKay M, Molloy M. Proteomics of Smad4 regulated transforming growth factor-beta signalling in colon cancer cells. Mol Biosyst. 2010;6:2332-8 pubmed publisher
Song K, Wang H, Krebs T, Wang B, Kelley T, Danielpour D. DHT selectively reverses Smad3-mediated/TGF-beta-induced responses through transcriptional down-regulation of Smad3 in prostate epithelial cells. Mol Endocrinol. 2010;24:2019-29 pubmed publisher
Liu Y, Kawai K, Khashabi S, Deng C, Liu Y, Yiu S. Inactivation of Smad4 leads to impaired ocular development and cataract formation. Biochem Biophys Res Commun. 2010;400:476-82 pubmed publisher
Langeveld D, van Hattem W, de Leng W, Morsink F, ten Kate F, Giardiello F, et al. SMAD4 immunohistochemistry reflects genetic status in juvenile polyposis syndrome. Clin Cancer Res. 2010;16:4126-34 pubmed publisher
Monceau V, Pasinetti N, Schupp C, Pouzoulet F, Opolon P, Vozenin M. Modulation of the Rho/ROCK pathway in heart and lung after thorax irradiation reveals targets to improve normal tissue toxicity. Curr Drug Targets. 2010;11:1395-404 pubmed
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product information
Product Name :
Smad4 (B-8) Antibody
Gene Name :
SMAD4
Size :
200 µg/ml
Catalog Number :
sc-7966
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Smad4
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
FL (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA