| Published Application/Species/Sample/Dilution | Reference | 
|---|
| western blot knockout validation; mouse; 1:1000; fig 7
 | Morris C, Stanton M, Manthey K, Oh K, Wagner K. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS ONE. 2012;7:e34308 pubmed  publisher | 
| western blot; human; 1:1000; loading ...; fig 1c
 | Xie F, Zhou X, Su P, Li H, Tu Y, Du J, et al . Breast cancer cell-derived extracellular vesicles promote CD8+ T cell exhaustion via TGF-β type II receptor signaling. Nat Commun. 2022;13:4461 pubmed  publisher | 
| western blot; mouse; 1:200; loading ...; fig 1a
 | Cheng Z, Naga Srikanth Garikipati V, Truongcao M, CIMINI M, Huang G, Wang C, et al . Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2. J Am Heart Assoc. 2021;10:e019755 pubmed  publisher | 
| immunocytochemistry; mouse; 1:100; loading ...; fig 7d
 | Little J, McNeely K, Michel N, Bott C, Lettieri K, Hecht M, et al . Loss of Coiled-Coil Protein Cep55 Impairs Neural Stem Cell Abscission and Results in p53-Dependent Apoptosis in Developing Cortex. J Neurosci. 2021;41:3344-3365 pubmed  publisher | 
| western blot; human; loading ...; fig 1a
 | Cordonnier M, Nardin C, Chanteloup G, Derangère V, Algros M, Arnould L, et al . Tracking the evolution of circulating exosomal-PD-L1 to monitor melanoma patients. J Extracell Vesicles. 2020;9:1710899 pubmed  publisher | 
| western blot; human; loading ...; fig 1b
 | Wan Z, Zhao L, Lu F, Gao X, Dong Y, Zhao Y, et al . Mononuclear phagocyte system blockade improves therapeutic exosome delivery to the myocardium. Theranostics. 2020;10:218-230 pubmed  publisher | 
| western blot; human; 1:100; loading ...; fig 2e
 | Geeurickx E, Tulkens J, Dhondt B, Van Deun J, Lippens L, Vergauwen G, et al . The generation and use of recombinant extracellular vesicles as biological reference material. Nat Commun. 2019;10:3288 pubmed  publisher | 
| western blot; human; 1:200; loading ...; fig 2f, 5c
 | Javidi Sharifi N, Martinez J, English I, Joshi S, Scopim Ribeiro R, Viola S, et al . FGF2-FGFR1 signaling regulates release of Leukemia-Protective exosomes from bone marrow stromal cells. elife. 2019;8: pubmed  publisher | 
| western blot; human; loading ...; fig s8
 | Skowyra M, Schlesinger P, Naismith T, Hanson P. Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Science. 2018;360: pubmed  publisher | 
| western blot; mouse; loading ...; fig 2i
 | Wan Y, Wang L, Zhu C, Zheng Q, Wang G, Tong J, et al . Aptamer-Conjugated Extracellular Nanovesicles for Targeted Drug Delivery. Cancer Res. 2018;78:798-808 pubmed  publisher | 
| western blot; human; 1:200; loading ...; fig 1g
 | Mrowczynski O, Madhankumar A, Slagle Webb B, Lee S, Zacharia B, Connor J. HFE genotype affects exosome phenotype in cancer. Biochim Biophys Acta Gen Subj. 2017;1861:1921-1928 pubmed  publisher | 
| western blot; human; 1:1000; loading ...; fig s2a
 | Bednash J, Weathington N, Londino J, Rojas M, Gulick D, Fort R, et al . Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nat Commun. 2017;8:15203 pubmed  publisher | 
| western blot; human; fig 7e
 | VALLABHAJOSYULA P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan C, et al . Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. J Clin Invest. 2017;127:1375-1391 pubmed  publisher | 
| western blot; human; 1:200; fig 2i
 | Chiang C, Flint M, Lin J, Spiropoulou C. Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells. PLoS ONE. 2016;11:e0164768 pubmed  publisher | 
| western blot; human; loading ...; fig 1e
 | Puhka M, Nordberg M, Valkonen S, Rannikko A, Kallioniemi O, Siljander P, et al . KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine. Eur J Pharm Sci. 2017;98:30-39 pubmed  publisher | 
| immunoprecipitation; human; loading ...; fig 5aimmunocytochemistry; human; loading ...; fig 3awestern blot; human; loading ...; fig 1a
 | Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al . ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed  publisher | 
| western blot; mouse; loading ...; fig 1b
 | Dinkins M, Enasko J, Hernandez C, Wang G, Kong J, Helwa I, et al . Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse. J Neurosci. 2016;36:8653-67 pubmed  publisher | 
| immunoprecipitation; mouse; loading ...; fig 6dwestern blot; mouse; loading ...; fig 5awestern blot; human; loading ...; fig 2c
 | Diaz Hidalgo L, Altuntas S, Rossin F, D Eletto M, Marsella C, Farrace M, et al . Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions. Biochim Biophys Acta. 2016;1863:2084-92 pubmed  publisher | 
| western blot; human; fig 1
 | Rider M, Hurwitz S, Meckes D. ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles. Sci Rep. 2016;6:23978 pubmed  publisher | 
| western blot; human; fig 1
 | Conigliaro A, Costa V, Lo Dico A, Saieva L, Buccheri S, Dieli F, et al . CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA. Mol Cancer. 2015;14:155 pubmed  publisher | 
| western blot; human; 1:1000; fig 1
 | Li H, Han L, Yang Z, Huang W, Zhang X, Gu Y, et al . Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS. Cell Physiol Biochem. 2015;36:1116-30 pubmed  publisher | 
| western blot; human; fig s3
 | Menck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, et al . Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN. J Mol Cell Biol. 2015;7:143-53 pubmed  publisher | 
| western blot; human; 1:1000
 | Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele J, et al . The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles. 2014;3: pubmed  publisher | 
| western blot; human; fig 1
 | Cypryk W, Ohman T, Eskelinen E, Matikainen S, Nyman T. Quantitative proteomics of extracellular vesicles released from human monocyte-derived macrophages upon ?-glucan stimulation. J Proteome Res. 2014;13:2468-77 pubmed  publisher | 
| immunocytochemistry; African green monkey; loading ...; fig s1a
 | Van Engelenburg S, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan S, et al . Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Science. 2014;343:653-6 pubmed  publisher | 
|  | Wu C, Li Z, Feng G, Wang L, Xie J, Jin Y, et al . Tumor suppressing role of serum-derived exosomal microRNA-15a in osteosarcoma cells through the GATA binding protein 2/murine double minute 2 axis and the p53 signaling pathway. Bioengineered. 2021;12:8378-8395 pubmed  publisher | 
|  | Coudert L, Osseni A, Gangloff Y, Schaeffer L, Leblanc P. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways. BMC Biol. 2021;19:153 pubmed  publisher | 
|  | Martínez Greene J, Hernández Ortega K, Quiroz Baez R, Resendis Antonio O, Pichardo Casas I, Sinclair D, et al . Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography. J Extracell Vesicles. 2021;10:e12087 pubmed  publisher | 
|  | Hettich B, Ben Yehuda Greenwald M, Werner S, Leroux J. Exosomes for Wound Healing: Purification Optimization and Identification of Bioactive Components. Adv Sci (Weinh). 2020;7:2002596 pubmed  publisher | 
|  | Lauritzen I, Bécot A, Bourgeois A, Pardossi Piquard R, Biferi M, Barkats M, et al . Targeting γ-secretase triggers the selective enrichment of oligomeric APP-CTFs in brain extracellular vesicles from Alzheimer cell and mouse models. Transl Neurodegener. 2019;8:35 pubmed  publisher | 
|  | Karasmanis E, Hwang D, Nakos K, Bowen J, Angelis D, Spiliotis E. A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. Curr Biol. 2019;: pubmed  publisher | 
|  | Fulzele S, Mendhe B, Khayrullin A, Johnson M, Kaiser H, Liu Y, et al . Muscle-derived miR-34a increases with age in circulating extracellular vesicles and induces senescence of bone marrow stem cells. Aging (Albany NY). 2019;11:1791-1803 pubmed  publisher | 
|  | Nehls J, Businger R, Hoffmann M, Brinkmann C, Fehrenbacher B, Schaller M, et al . Release of Immunomodulatory Ebola Virus Glycoprotein-Containing Microvesicles Is Suppressed by Tetherin in a Species-Specific Manner. Cell Rep. 2019;26:1841-1853.e6 pubmed  publisher | 
|  | Kim J, Afshari A, Sengupta R, Sebastiano V, Gupta A, Kim Y, et al . Replication study: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. elife. 2018;7: pubmed  publisher | 
|  | Myles I, Anderson E, Earland N, Zarember K, Sastalla I, Williams K, et al . TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome. J Clin Invest. 2018;128:3595-3604 pubmed  publisher | 
|  | Abdulrahman B, Abdelaziz D, Schatzl H. Autophagy regulates exosomal release of prions in neuronal cells. J Biol Chem. 2018;293:8956-8968 pubmed  publisher | 
|  | Zhang Y, Kim M, Jia B, Yan J, Zuniga Hertz J, Han C, et al . Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature. 2017;548:52-57 pubmed  publisher | 
|  | Mehta A, Cordero J, Dobersch S, Romero Olmedo A, Savai R, Bodner J, et al . Non-invasive lung cancer diagnosis by detection of GATA6 and NKX2-1 isoforms in exhaled breath condensate. EMBO Mol Med. 2016;8:1380-1389 pubmed  publisher | 
|  | Song X, Ding Y, Liu G, Yang X, Zhao R, Zhang Y, et al . Cancer Cell-derived Exosomes Induce Mitogen-activated Protein Kinase-dependent Monocyte Survival by Transport of Functional Receptor Tyrosine Kinases. J Biol Chem. 2016;291:8453-64 pubmed  publisher | 
|  | Lesnik J, ANTES T, Kim J, Griner E, Pedro L. Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. elife. 2016;5:e07383 pubmed  publisher | 
|  | Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, et al . Mechanism of Ca²âº-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun. 2014;5:5646 pubmed  publisher | 
|  | Faresse N, Debonneville A, Staub O. USP2-45 represses aldosterone mediated responses by decreasing mineralocorticoid receptor availability. Cell Physiol Biochem. 2013;31:462-72 pubmed  publisher | 
|  | Huang S, Wu C, Chang Y, Kwon Chung K, Brown R, Jong A. Cryptococcus neoformans-derived microvesicles enhance the pathogenesis of fungal brain infection. PLoS ONE. 2012;7:e48570 pubmed  publisher | 
|  | Lee H, Koo B, Kim Y, Jeon O, Kim D. Exosome release of ADAM15 and the functional implications of human macrophage-derived ADAM15 exosomes. FASEB J. 2012;26:3084-95 pubmed  publisher | 
|  | Ruiss R, Jochum S, Mocikat R, Hammerschmidt W, Zeidler R. EBV-gp350 confers B-cell tropism to tailored exosomes and is a neo-antigen in normal and malignant B cells--a new option for the treatment of B-CLL. PLoS ONE. 2011;6:e25294 pubmed  publisher | 
|  | Huang S, Wu C, Jiang S, Bahner I, Lossinsky A, Jong A. HIV-1 gp41 ectodomain enhances Cryptococcus neoformans binding to human brain microvascular endothelial cells via gp41 core-induced membrane activities. Biochem J. 2011;438:457-66 pubmed  publisher | 
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|  | Jong A, Wu C, Shackleford G, Kwon Chung K, Chang Y, Chen H, et al . Involvement of human CD44 during Cryptococcus neoformans infection of brain microvascular endothelial cells. Cell Microbiol. 2008;10:1313-26 pubmed  publisher | 
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