product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
SCP-3 (D-1) Antibody
catalog :
sc-74569
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D-1
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; fig 2b
Rivero Hinojosa S, Pugacheva E, Kang S, Mendez Catala C, Kovalchuk A, Strunnikov A, et al. The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis. Nat Commun. 2021;12:3846 pubmed publisher
  • immunocytochemistry; rat; fig 7e
  • western blot; rat; 1:50; fig 1b
Mihola O, Landa V, Pratto F, Brick K, Kobets T, Kusari F, et al. Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility. BMC Biol. 2021;19:86 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig s2-1d
Felipe Medina N, Caburet S, Sánchez Sáez F, Condezo Y, de Rooij D, Gómez H L, et al. A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. elife. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250; loading ...; fig 2s2f
Kojima M, de Rooij D, Page D. Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7c
Katsumata O, Mori M, Sawane Y, Niimura T, Ito A, Okamoto H, et al. Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues. Histochem Cell Biol. 2017;148:577-596 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 3c
Liu W, Wang F, Xu Q, Shi J, Zhang X, Lu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3g
Hansen R, Mund A, Poulsen S, Sandoval M, Klement K, Tsouroula K, et al. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016;18:1357-1366 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4a
Gómez H L, Felipe Medina N, Sanchez Martin M, Davies O, Ramos I, García Tuñón I, et al. C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility. Nat Commun. 2016;7:13298 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 5
Li M, Cole F, Patel D, Misenko S, Her J, Malhowski A, et al. 53BP1 ablation rescues genomic instability in mice expressing 'RING-less' BRCA1. EMBO Rep. 2016;17:1532-1541 pubmed
  • immunocytochemistry; mouse; 1:50; fig 5
Fu C, Begum K, Jordan P, He Y, Overbeek P. Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS ONE. 2016;11:e0159800 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 5a
Mak W, Fang C, Holden T, Dratver M, Lin H. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline. Biol Reprod. 2016;94:134 pubmed publisher
  • immunocytochemistry; mouse; fig 3b
Liu Y, Liu C, Chang Z, Wadas B, Brower C, Song Z, et al. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016;291:7426-38 pubmed publisher
  • immunohistochemistry; mouse
Davies B, Hatton E, Altemose N, Hussin J, Pratto F, Zhang G, et al. Re-engineering the zinc fingers of PRDM9 reverses hybrid sterility in mice. Nature. 2016;530:171-176 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 4
  • immunocytochemistry; mouse; 1:200; fig 4
Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, et al. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. PLoS ONE. 2015;10:e0143148 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4a
Faieta M, Di Cecca S, de Rooij D, Luchetti A, Murdocca M, Di Giacomo M, et al. A surge of late-occurring meiotic double-strand breaks rescues synapsis abnormalities in spermatocytes of mice with hypomorphic expression of SPO11. Chromosoma. 2016;125:189-203 pubmed publisher
  • immunocytochemistry; mouse; fig 4
Baker C, Petkova P, Walker M, Flachs P, Mihola O, Trachtulec Z, et al. Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots. PLoS Genet. 2015;11:e1005512 pubmed publisher
  • western blot; mouse; 1:1000; fig 5
Tassinari V, Campolo F, Cesarini V, Todaro F, Dolci S, Rossi P. Fgf9 inhibition of meiotic differentiation in spermatogonia is mediated by Erk-dependent activation of Nodal-Smad2/3 signaling and is antagonized by Kit Ligand. Cell Death Dis. 2015;6:e1688 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig 1a
Vrooman L, Oatley J, Griswold J, Hassold T, Hunt P. Estrogenic exposure alters the spermatogonial stem cells in the developing testis, permanently reducing crossover levels in the adult. PLoS Genet. 2015;11:e1004949 pubmed publisher
  • immunocytochemistry; human; 1:100
Petit F, Kervarrec C, Jamin S, Smagulova F, Hao C, Becker E, et al. Combining RNA and protein profiling data with network interactions identifies genes associated with spermatogenesis in mouse and human. Biol Reprod. 2015;92:71 pubmed publisher
  • immunohistochemistry - paraffin section; human
Simhadri S, Peterson S, Patel D, Huo Y, Cai H, Bowman Colin C, et al. Male fertility defect associated with disrupted BRCA1-PALB2 interaction in mice. J Biol Chem. 2014;289:24617-29 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Jansa P, Homolka D, Blatny R, Mistrik M, Bartek J, Forejt J. Dosage compensation of an aneuploid genome in mouse spermatogenic cells. Biol Reprod. 2014;90:124 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200
Okumura L, Lesch B, Page D. The ligand binding domain of GCNF is not required for repression of pluripotency genes in mouse fetal ovarian germ cells. PLoS ONE. 2013;8:e66062 pubmed publisher
Brick K, Thibault Sennett S, Smagulova F, Lam K, Pu Y, Pratto F, et al. Extensive sex differences at the initiation of genetic recombination. Nature. 2018;561:338-342 pubmed publisher
Gregorova S, Gergelits V, Chvatalova I, Bhattacharyya T, Valiskova B, Fotopulosova V, et al. Modulation of Prdm9-controlled meiotic chromosome asynapsis overrides hybrid sterility in mice. elife. 2018;7: pubmed publisher
Wang L, Tu Z, Liu C, Liu H, Kaldis P, Chen Z, et al. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ. 2018;25:1174-1188 pubmed publisher
Kent T, Arnold S, Fasnacht R, Rowsey R, Mitchell D, Hogarth C, et al. ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle, and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes. Biol Reprod. 2016;94:12 pubmed publisher
Urriola Muñoz P, Lagos Cabré R, Moreno R. A mechanism of male germ cell apoptosis induced by bisphenol-A and nonylphenol involving ADAM17 and p38 MAPK activation. PLoS ONE. 2014;9:e113793 pubmed publisher
Uechi H, Hamazaki J, Murata S. Characterization of the testis-specific proteasome subunit ?4s in mammals. J Biol Chem. 2014;289:12365-74 pubmed publisher
Dowdle J, Mehta M, Kass E, Vuong B, Inagaki A, Egli D, et al. Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. PLoS Genet. 2013;9:e1003945 pubmed publisher
Ngo D, Cheng Q, O Connor A, Deboer K, Lo C, Beaulieu E, et al. Glucocorticoid-induced leucine zipper (GILZ) regulates testicular FOXO1 activity and spermatogonial stem cell (SSC) function. PLoS ONE. 2013;8:e59149 pubmed publisher
Jamsai D, O Connor A, Deboer K, Clark B, Smith S, Browne C, et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS ONE. 2013;8:e56955 pubmed publisher
Cole F, Kauppi L, Lange J, Roig I, Wang R, Keeney S, et al. Homeostatic control of recombination is implemented progressively in mouse meiosis. Nat Cell Biol. 2012;14:424-30 pubmed publisher
Fukuda T, Pratto F, Schimenti J, Turner J, Camerini Otero R, Hoog C. Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. PLoS Genet. 2012;8:e1002485 pubmed publisher
product information
Product Name :
SCP-3 (D-1) Antibody
Size :
200 µg/ml
Catalog Number :
sc-74569
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
SCP-3
reactive species  :
m, r
Application :
WB, IP, IF, ELISA
Epitope :
FL (m)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA