product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Raf-1 (E-10) Antibody
catalog :
sc-7267
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E-10
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, proximity ligation assay
citations: 31
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3b
Qi Z, Xu H, Zhang S, Xu J, Li S, Gao H, et al. RIPK4/PEBP1 axis promotes pancreatic cancer cell migration and invasion by activating RAF1/MEK/ERK signaling. Int J Oncol. 2018;52:1105-1116 pubmed publisher
  • western blot; human; loading ...; fig 4a
Ferraiuolo R, Tubman J, Sinha I, Hamm C, Porter L. The cyclin-like protein, SPY1, regulates the ER? and ERK1/2 pathways promoting tamoxifen resistance. Oncotarget. 2017;8:23337-23352 pubmed publisher
  • immunoprecipitation; human; 1:1000; fig 4
  • immunoprecipitation; mouse; 1:1000; fig 2
Bunda S, Burrell K, Heir P, Zeng L, Alamsahebpour A, Kano Y, et al. Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis. Nat Commun. 2015;6:8859 pubmed publisher
  • western blot; mouse; fig 4
Boswell B, Musil L. Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells. Mol Biol Cell. 2015;26:2561-72 pubmed publisher
  • proximity ligation assay; human; 1:100; fig 8
Brobeil A, Kämmerer F, Tag C, Steger K, Gattenlöhner S, Wimmer M. PTPIP51—A New RelA-tionship with the NFκB Signaling Pathway. Biomolecules. 2015;5:485-504 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 6g
Yang Z, Zheng B, Zhang Y, He M, Zhang X, Ma D, et al. miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells. Biochim Biophys Acta. 2015;1852:1477-89 pubmed publisher
  • proximity ligation assay; mouse
  • immunohistochemistry - paraffin section; mouse
Brobeil A, Viard M, Petri M, Steger K, Tag C, Wimmer M. Memory and PTPIP51--a new protein in hippocampus and cerebellum. Mol Cell Neurosci. 2015;64:61-73 pubmed publisher
  • western blot; human
Petti C, Picco G, Martelli M, Trisolini E, Bucci E, Perera T, et al. Truncated RAF kinases drive resistance to MET inhibition in MET-addicted cancer cells. Oncotarget. 2015;6:221-33 pubmed
  • proximity ligation assay; human; 1:100; loading ...; fig 9d
Brobeil A, Graf M, Eiber M, Wimmer M. Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules. 2012;2:122-42 pubmed publisher
Hinterseher I, Schworer C, Lillvis J, Stahl E, Erdman R, Gatalica Z, et al. Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci. 2015;16:11196-212 pubmed publisher
Wada M, Horinaka M, Yamazaki T, Katoh N, Sakai T. The dual RAF/MEK inhibitor CH5126766/RO5126766 may be a potential therapy for RAS-mutated tumor cells. PLoS ONE. 2014;9:e113217 pubmed publisher
Minna E, Romeo P, De Cecco L, Dugo M, Cassinelli G, Pilotti S, et al. miR-199a-3p displays tumor suppressor functions in papillary thyroid carcinoma. Oncotarget. 2014;5:2513-28 pubmed
Brobeil A, Bobrich M, Tag C, Wimmer M. PTPIP51 in protein interactions: regulation and in situ interacting partners. Cell Biochem Biophys. 2012;63:211-22 pubmed publisher
Zuco V, De Cesare M, Cincinelli R, Nannei R, Pisano C, Zaffaroni N, et al. Synergistic antitumor effects of novel HDAC inhibitors and paclitaxel in vitro and in vivo. PLoS ONE. 2011;6:e29085 pubmed publisher
Balgi A, Diering G, Donohue E, Lam K, Fonseca B, Zimmerman C, et al. Regulation of mTORC1 signaling by pH. PLoS ONE. 2011;6:e21549 pubmed publisher
Yang H, Higgins B, Kolinsky K, Packman K, Go Z, Iyer R, et al. RG7204 (PLX4032), a selective BRAFV600E inhibitor, displays potent antitumor activity in preclinical melanoma models. Cancer Res. 2010;70:5518-27 pubmed publisher
Halaban R, Zhang W, Bacchiocchi A, Cheng E, Parisi F, Ariyan S, et al. PLX4032, a selective BRAF(V600E) kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAF melanoma cells. Pigment Cell Melanoma Res. 2010;23:190-200 pubmed publisher
Shu F, Ramineni S, Hepler J. RGS14 is a multifunctional scaffold that integrates G protein and Ras/Raf MAPkinase signalling pathways. Cell Signal. 2010;22:366-76 pubmed publisher
Koch P, Petri M, Paradowska A, Stenzinger A, Sturm K, Steger K, et al. PTPIP51 mRNA and protein expression in tissue microarrays and promoter methylation of benign prostate hyperplasia and prostate carcinoma. Prostate. 2009;69:1751-62 pubmed publisher
Webster R, Giles K, Price K, Zhang P, Mattick J, Leedman P. Regulation of epidermal growth factor receptor signaling in human cancer cells by microRNA-7. J Biol Chem. 2009;284:5731-41 pubmed publisher
Zuco V, Zunino F. Cyclic pifithrin-alpha sensitizes wild type p53 tumor cells to antimicrotubule agent-induced apoptosis. Neoplasia. 2008;10:587-96 pubmed
Mao X, Orchard G, Mitchell T, Oyama N, Russell Jones R, Vermeer M, et al. A genomic and expression study of AP-1 in primary cutaneous T-cell lymphoma: evidence for dysregulated expression of JUNB and JUND in MF and SS. J Cutan Pathol. 2008;35:899-910 pubmed publisher
Mao X, Orchard G. Abnormal AP-1 protein expression in primary cutaneous B-cell lymphomas. Br J Dermatol. 2008;159:145-51 pubmed publisher
Engebretsen B, Irwin D, Valdez M, O Donovan M, Tucker A, van Patot M. Acute hypobaric hypoxia (5486 m) induces greater pulmonary HIF-1 activation in hilltop compared to madison rats. High Alt Med Biol. 2007;8:312-21 pubmed
Provot S, Nachtrab G, Paruch J, Chen A, Silva A, Kronenberg H. A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes. Mol Cell Biol. 2008;28:344-57 pubmed
Mao X, Orchard G, Russell Jones R, Whittaker S. Abnormal activator protein 1 transcription factor expression in CD30-positive cutaneous large-cell lymphomas. Br J Dermatol. 2007;157:914-21 pubmed
Methi T, Ngai J, Vang T, Torgersen K, Tasken K. Hypophosphorylated TCR/CD3zeta signals through a Grb2-SOS1-Ras pathway in Lck knockdown cells. Eur J Immunol. 2007;37:2539-48 pubmed
Yokoyama T, Takano K, Yoshida A, Katada F, Sun P, Takenawa T, et al. DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras-ERK pathway, is required for myogenic differentiation. J Cell Biol. 2007;177:781-93 pubmed
Romanenko A, Morimura K, Kinoshita A, Wanibuchi H, Takahashi S, Zaparin W, et al. Upregulation of fibroblast growth factor receptor 3 and epidermal growth factor receptors, in association with Raf-1, in urothelial dysplasia and carcinoma in situ after the Chernobyl accident. Cancer Sci. 2006;97:1168-74 pubmed
Adams D, Coffee R, Zhang H, Pelech S, Strack S, Wadzinski B. Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes. J Biol Chem. 2005;280:42644-54 pubmed
Mukherjee R, Bartlett J, Krishna N, Underwood M, Edwards J. Raf-1 expression may influence progression to androgen insensitive prostate cancer. Prostate. 2005;64:101-7 pubmed
product information
Product Name :
Raf-1 (E-10) Antibody
Gene Name :
RAF1
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-7267
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Raf-1
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
C-terminal (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA