product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Pol II (8WG16) Antibody
catalog :
sc-56767
quantity :
200 µg/ml
price :
333 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8WG16
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay
citations: 30
Published Application/Species/Sample/DilutionReference
  • proximity ligation assay; human; 1:100; loading ...; fig 4g
Park K, Ryoo J, Jeong H, Kim M, Lee S, Hwang S, et al. Aicardi-Goutières syndrome-associated gene SAMHD1 preserves genome integrity by preventing R-loop formation at transcription-replication conflict regions. PLoS Genet. 2021;17:e1009523 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 3c
  • western blot; human; loading ...; fig 3a
Yu D, Cattoglio C, Xue Y, Zhou Q. A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Nucleic Acids Res. 2019;47:4462-4475 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 4h
Blank M, Chen S, Poetz F, Schnolzer M, Voit R, Grummt I. SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription. Nucleic Acids Res. 2017;45:2675-2686 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig s2a
Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed publisher
  • western blot; mouse; fig 7
Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res. 2016;44:8933-8950 pubmed
  • chromatin immunoprecipitation; mouse
Perdigão Henriques R, Petrocca F, Altschuler G, Thomas M, Le M, Tan S, et al. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Oncogene. 2016;35:158-72 pubmed publisher
  • chromatin immunoprecipitation; human
Sengupta S, Biarnes M, Clarke R, Jordan V. Inhibition of BET proteins impairs estrogen-mediated growth and transcription in breast cancers by pausing RNA polymerase advancement. Breast Cancer Res Treat. 2015;150:265-78 pubmed publisher
  • chromatin immunoprecipitation; mouse
Gjidoda A, Tagore M, McAndrew M, Woods A, Floer M. Nucleosomes are stably evicted from enhancers but not promoters upon induction of certain pro-inflammatory genes in mouse macrophages. PLoS ONE. 2014;9:e93971 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Morales J, Richard P, Rommel A, Fattah F, Motea E, Patidar P, et al. Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair. Nucleic Acids Res. 2014;42:4996-5006 pubmed publisher
Chen Y, Zhou T, Liao Z, Gao W, Wu J, Zhang S, et al. Hnrnpk is essential for embryonic limb bud development as a transcription activator and a collaborator of insulator protein Ctcf. Cell Death Differ. 2023;30:2293-2308 pubmed publisher
Patel P, Algouneh A, Krishnan R, Reynolds J, Nixon K, Hao J, et al. Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancers. Nucleic Acids Res. 2023;51:4341-4362 pubmed publisher
Offley S, Pfleiderer M, Zucco A, Fraudeau A, Welsh S, Razew M, et al. A combinatorial approach to uncover an additional Integrator subunit. Cell Rep. 2023;42:112244 pubmed publisher
Kim H, Jang S, Lee Y. The m6A(m)-independent role of FTO in regulating WNT signaling pathways. Life Sci Alliance. 2022;5: pubmed publisher
Kant S, Tran K, Kvandová M, Caliz A, Yoo H, Learnard H, et al. PGC1α Regulates the Endothelial Response to Fluid Shear Stress via Telomerase Reverse Transcriptase Control of Heme Oxygenase-1. Arterioscler Thromb Vasc Biol. 2022;42:19-34 pubmed publisher
Hancock M, Meyer R, Mistry M, Khetani R, Wagschal A, Shin T, et al. Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression. Cell. 2019;177:722-736.e22 pubmed publisher
Wu D, De Wever V, Derua R, Winkler C, Beullens M, Van Eynde A, et al. A substrate-trapping strategy for protein phosphatase PP1 holoenzymes using hypoactive subunit fusions. J Biol Chem. 2018;293:15152-15162 pubmed publisher
Hsu E, Pennella M, Zemke N, Eng C, Berk A. Adenovirus E1A Activation Domain Regulates H3 Acetylation Affecting Varied Steps in Transcription at Different Viral Promoters. J Virol. 2018;92: pubmed publisher
Teves S, An L, Bhargava Shah A, Xie L, Darzacq X, Tjian R. A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes. elife. 2018;7: pubmed publisher
Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, et al. Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nat Cell Biol. 2017;19:1400-1411 pubmed publisher
Hanssen L, Kassouf M, Oudelaar A, Biggs D, Preece C, Downes D, et al. Tissue-specific CTCF-cohesin-mediated chromatin architecture delimits enhancer interactions and function in vivo. Nat Cell Biol. 2017;19:952-961 pubmed publisher
Gómez Herreros F, Margaritis T, Rodríguez Galán O, Pelechano V, Begley V, Millán Zambrano G, et al. The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation. Nucleic Acids Res. 2017;45:9302-9318 pubmed publisher
Banyai G, Szilagyi Z, Baraznenok V, Khorosjutina O, Gustafsson C. Cyclin C influences the timing of mitosis in fission yeast. Mol Biol Cell. 2017;28:1738-1744 pubmed publisher
Garavís M, González Polo N, Allepuz Fuster P, Louro J, Fernández Tornero C, Calvo O. Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis. Nucleic Acids Res. 2017;45:2458-2471 pubmed publisher
Esnault C, Stewart A, Gualdrini F, East P, Horswell S, Matthews N, et al. Rho-actin signaling to the MRTF coactivators dominates the immediate transcriptional response to serum in fibroblasts. Genes Dev. 2014;28:943-58 pubmed publisher
Li Z, Tian T, Lv F, Chang Y, Wang X, Zhang L, et al. Six1 promotes proliferation of pancreatic cancer cells via upregulation of cyclin D1 expression. PLoS ONE. 2013;8:e59203 pubmed publisher
Adhikary T, Brandt D, Kaddatz K, Stockert J, Naruhn S, Meissner W, et al. Inverse PPAR?/? agonists suppress oncogenic signaling to the ANGPTL4 gene and inhibit cancer cell invasion. Oncogene. 2013;32:5241-52 pubmed publisher
Schwer B, Sanchez A, Shuman S. Punctuation and syntax of the RNA polymerase II CTD code in fission yeast. Proc Natl Acad Sci U S A. 2012;109:18024-9 pubmed publisher
Tolde O, Folk P. Stress-induced expression of p53 target genes is insensitive to SNW1/SKIP downregulation. Cell Mol Biol Lett. 2011;16:373-84 pubmed publisher
O Shea J, Perkins N. Thr435 phosphorylation regulates RelA (p65) NF-kappaB subunit transactivation. Biochem J. 2010;426:345-54 pubmed publisher
Xiong H, Qian J, He T, Li F. Independent transcription of miR-281 in the intron of ODA in Drosophila melanogaster. Biochem Biophys Res Commun. 2009;378:883-9 pubmed publisher
product information
Catalog Number :
sc-56767
Product Name :
Pol II (8WG16) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
8WG16
Isotype :
IgG2a
Size :
200 µg/ml
List Price :
333 USD
Immunogen :
RNA polymerase II
Specificity :
mouse, rat, human, Xenopus, C. elegans, yeast, T. aestivum, bovine
Uses :
WB, IP, IF
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA