product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Oct-3/4 (C-10) Antibody
catalog :
sc-5279
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C-10
reactivity :
African green monkey, human, mouse, rat, dogs, bovine, pigs , Xenopus laevis, domestic rabbit, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, ChIP-Seq, In-Cell Western, RNA immunoprecipitation, other, western blot knockout validation
citations: 714
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:1000; fig s3
  • RNA immunoprecipitation; mouse; fig 7
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig 4
  • immunohistochemistry; mouse; 1:100; fig 1
Boo K, Bhin J, Jeon Y, Kim J, Shin H, Park J, et al. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells. Nat Commun. 2015;6:6810 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig s2c
Han Y, Tan L, Zhou T, Yang L, Carrau L, Lacko L, et al. A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants. Cell Stem Cell. 2022;29:1475-1490.e6 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:20; loading ...; fig 1a
Chen Y, Lu C, Cheng W, Kuo K, Yu C, Ho H, et al. An experimental model for ovarian cancer: propagation of ovarian cancer initiating cells and generation of ovarian cancer organoids. BMC Cancer. 2022;22:967 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1e
Meng Y, Wang G, He H, Lau K, Hurt A, Bixler B, et al. Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenome. Nat Cell Biol. 2022;24:1141-1153 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 6e
Chao J, Feng L, Ye P, Chen X, Cui Q, Sun G, et al. Therapeutic development for Canavan disease using patient iPSCs introduced with the wild-type ASPA gene. iScience. 2022;25:104391 pubmed publisher
  • immunocytochemistry; human; 1:250; loading ...; fig s2b
Schembs L, Willems A, Hasenpusch Theil K, Cooper J, Whiting K, Burr K, et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. Cell Rep. 2022;39:110811 pubmed publisher
  • immunocytochemistry; human; fig 5c
Liu F, Han Q, Zhang T, Chang F, Deng J, Huang X, et al. CRL4-DCAF8L1 Regulates BRCA1 and BARD1 Protein Stability. Int J Biol Sci. 2022;18:1434-1450 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1b
  • western blot; human; loading ...; fig s4j
Sivakumar S, Qi S, Cheng N, Sathe A, Kanchwala M, Kumar A, et al. TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling. Cell Rep. 2022;38:110395 pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 2i
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2b, 2c
  • western blot; mouse; loading ...; fig 4g
Han D, Wu G, Chen R, Drexler H, MacCarthy C, Kim K, et al. A balanced Oct4 interactome is crucial for maintaining pluripotency. Sci Adv. 2022;8:eabe4375 pubmed publisher
  • western blot; human; 1:100; fig 3d
Castillo P, Aisagbonhi O, Saenz C, ElShamy W. Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: the case for longer breastfeeding. Am J Cancer Res. 2022;12:396-426 pubmed
  • western blot; pigs ; fig 3c
Yu S, Zhang R, Shen Q, Zhu Z, Zhang J, Wu X, et al. ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2. Front Cell Dev Biol. 2021;9:712224 pubmed publisher
  • immunohistochemistry; human; 1:50; fig 2a
Martin Inaraja M, Ferreira M, Taelman J, Eguizabal C, Chuva de Sousa Lopes S. Improving In Vitro Culture of Human Male Fetal Germ Cells. Cells. 2021;10: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2c
Deguchi S, Shintani T, Harada K, Okamoto T, Takemura A, Hirata K, et al. In Vitro Model for a Drug Assessment of Cytochrome P450 Family 3 Subfamily A Member 4 Substrates Using Human Induced Pluripotent Stem Cells and Genome Editing Technology. Hepatol Commun. 2021;5:1385-1399 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4e
Olbrich T, Vega Sendino M, Tillo D, Wu W, Zolnerowich N, Pavani R, et al. CTCF is a barrier for 2C-like reprogramming. Nat Commun. 2021;12:4856 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
Gholami S, Mazidi Z, Pahlavan S, Moslem F, Hosseini M, Taei A, et al. A Novel Insight into Endothelial and Cardiac Cells Phenotype in Systemic Sclerosis Using Patient-Derived Induced Pluripotent Stem Cell. Cell J. 2021;23:273-287 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 1b
Takeda H, Dondzillo A, Randall J, Gubbels S. Selective ablation of cochlear hair cells promotes engraftment of human embryonic stem cell-derived progenitors in the mouse organ of Corti. Stem Cell Res Ther. 2021;12:352 pubmed publisher
  • immunocytochemistry; mouse; 1:2000; loading ...; fig s2-1a
Minati L, Firrito C, Del Piano A, Peretti A, Sidoli S, Peroni D, et al. One-shot analysis of translated mammalian lncRNAs with AHARIBO. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...
Tang C, Han J, Dalvi S, Manian K, Winschel L, Volland S, et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol. 2021;4:161 pubmed publisher
  • western blot; human; 1:200; fig 2c
B xf6 hnke J, Pinkert S, Schmidt M, Binder H, Bilz N, Jung M, et al. Coxsackievirus B3 Infection of Human iPSC Lines and Derived Primary Germ-Layer Cells Regarding Receptor Expression. Int J Mol Sci. 2021;22: pubmed publisher
  • immunohistochemistry; human; 1:50; fig s1-1c
Ortiz Cordero C, Magli A, Dhoke N, Kuebler T, Selvaraj S, Oliveira N, et al. NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
Mo J, Anastasaki C, Chen Z, Shipman T, Papke J, Yin K, et al. Humanized neurofibroma model from induced pluripotent stem cells delineates tumor pathogenesis and developmental origins. J Clin Invest. 2020;: pubmed publisher
  • immunohistochemistry; mouse; 1:400; loading ...; fig 4f
Kyprianou C, Christodoulou N, Hamilton R, Nahaboo W, Boomgaard D, Amadei G, et al. Basement membrane remodelling regulates mouse embryogenesis. Nature. 2020;582:253-258 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1c
Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, et al. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Res. 2020;48:6001-6018 pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 2f
  • western blot; human; loading ...; fig 2g
Guo C, Ma X, Xing Y, Zheng C, Xu Y, Shan L, et al. Distinct Processing of lncRNAs Contributes to Non-conserved Functions in Stem Cells. Cell. 2020;181:621-636.e22 pubmed publisher
  • western blot; mouse; loading ...
Atashpaz S, Samadi Shams S, Gonzalez J, Sebestyén E, Arghavanifard N, Gnocchi A, et al. ATR expands embryonic stem cell fate potential in response to replication stress. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; 1:1000; loading ...; fig 1b
Choi I, Lim H, Cho H, Oh Y, Chou B, Bai H, et al. Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1e
Kawai K, Negoro R, Ichikawa M, Yamashita T, Deguchi S, Harada K, et al. Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies. Mol Ther Methods Clin Dev. 2020;17:49-57 pubmed publisher
  • immunohistochemistry; mouse; fig 3b
Sozen B, Cox A, De Jonghe J, Bao M, Hollfelder F, Glover D, et al. Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid. Dev Cell. 2019;51:698-712.e8 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 3s1b
Selvaraj S, Mondragón González R, Xu B, Magli A, Kim H, Laine J, et al. Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3d
Bredenkamp N, Yang J, Clarke J, Stirparo G, von Meyenn F, Dietmann S, et al. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports. 2019;13:1083-1098 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig e2a, e2d
  • western blot; mouse; 1:200; loading ...; fig e1c
Strebinger D, Deluz C, Friman E, Govindan S, Alber A, Suter D. Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions. Mol Syst Biol. 2019;15:e9002 pubmed publisher
  • immunohistochemistry; human; 1:100; fig 1b
Malerba N, Benzoni P, Squeo G, Milanesi R, Giannetti F, Sadleir L, et al. Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line. Stem Cell Res. 2019;40:101547 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s2c
Lee J, Termglinchan V, Diecke S, Itzhaki I, Lam C, Garg P, et al. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy. Nature. 2019;572:335-340 pubmed publisher
  • western blot; human; loading ...; fig 4g
Huang X, Wei C, Li F, Jia L, Zeng P, Li J, et al. PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions. Protein Cell. 2019;: pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1b
Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1d
Fu L, Hu Y, Song M, Liu Z, Zhang W, Yu F, et al. Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biol. 2019;17:e3000201 pubmed publisher
  • flow cytometry; human; 1:20; loading ...; fig s2c, s4b
Yap L, Wang J, Moreno Moral A, Chong L, Sun Y, Harmston N, et al. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Cell Rep. 2019;26:3231-3245.e9 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s1b
  • immunoprecipitation; human; loading ...; fig s2a
  • immunocytochemistry; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 1d
Wei X, Guo J, Li Q, Jia Q, Jing Q, Li Y, et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv. 2019;5:eaau7887 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 5h
  • western blot; human; 1:1000; loading ...; fig 2i
Mathieu J, Detraux D, Kuppers D, Wang Y, Cavanaugh C, Sidhu S, et al. Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency. Nat Commun. 2019;10:632 pubmed publisher
  • flow cytometry; human; 1:50; fig 1e
  • immunocytochemistry; human; 1:100; fig 1f
Xue Y, Liao B, Xie Y, Li S, Ma X, Sun X. Establishment of an ectodermal dysplasia related gene EDA Knockout human embryonic stem cell line (WAe001-A-22) by CRISPR-Cas9 technology. Stem Cell Res. 2019;34:101379 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig 3b
Kaindl J, Meiser I, Majer J, Sommer A, Krach F, Katsen Globa A, et al. Zooming in on Cryopreservation of hiPSCs and Neural Derivatives: A Dual-Center Study Using Adherent Vitrification. Stem Cells Transl Med. 2019;8:247-259 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 3a
Kim A, Lee E, Lee E, Kim J, Suk K, Lee E, et al. SIRT2 is required for efficient reprogramming of mouse embryonic fibroblasts toward pluripotency. Cell Death Dis. 2018;9:893 pubmed publisher
  • western blot; mouse; loading ...; fig s4c
Sutherland L, Ruhe M, Gattegno Ho D, Mann K, Greaves J, Koscielniak M, et al. LIF-dependent survival of embryonic stem cells is regulated by a novel palmitoylated Gab1 signalling protein. J Cell Sci. 2018;131: pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 1d
Klein T, Günther K, Kwok C, Edenhofer F, Uceyler N. Generation of the human induced pluripotent stem cell line (UKWNLi001-A) from skin fibroblasts of a woman with Fabry disease carrying the X-chromosomal heterozygous c.708 G > C (W236C) missense mutation in exon 5 of the alpha-galactosidase-A gene. Stem Cell Res. 2018;31:222-226 pubmed publisher
  • immunohistochemistry; mouse; 1:50; loading ...; fig 4a
Edwards N, Watson A, Betts D. Knockdown of p66Shc alters lineage-associated transcription factor expression in mouse blastocysts. Stem Cells Dev. 2018;: pubmed publisher
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 1d
Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H. Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells. Development. 2018;145: pubmed publisher
  • immunocytochemistry; human; loading ...; fig s6c
Wang C, Najm R, Xu Q, Jeong D, Walker D, Balestra M, et al. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. Nat Med. 2018;24:647-657 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 1b
Marrone L, Bus C, Schöndorf D, Fitzgerald J, Kübler M, Schmid B, et al. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease. PLoS ONE. 2018;13:e0192497 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 1f
Jansch C, Günther K, Waider J, Ziegler G, Forero A, Kollert S, et al. Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3. Stem Cell Res. 2018;28:136-140 pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 5c
Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 5a
Meng Y, Moore R, Tao W, Smith E, Tse J, Caslini C, et al. GATA6 phosphorylation by Erk1/2 propels exit from pluripotency and commitment to primitive endoderm. Dev Biol. 2018;436:55-65 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4g
Zhou Z, Wang L, Ge F, Gong P, Wang H, Wang F, et al. Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis. Nucleic Acids Res. 2018;46:3468-3486 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1e
Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y, et al. Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Stem Cell Res. 2018;26:67-71 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 1c
Tang L, Wang M, Liu D, Gong M, Ying Q, Ye S. Sp5 induces the expression of Nanog to maintain mouse embryonic stem cell self-renewal. PLoS ONE. 2017;12:e0185714 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig s10a
Chen X, Janssen J, Liu J, Maggio I, t Jong A, Mikkers H, et al. In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Nat Commun. 2017;8:657 pubmed publisher
  • immunocytochemistry; human; 1:60; fig 1g
Alonso Barroso E, Brasil S, Briso Montiano Á, Navarrete R, Perez Cerda C, Ugarte M, et al. Generation and characterization of a human iPSC line from a patient with propionic acidemia due to defects in the PCCA gene. Stem Cell Res. 2017;23:173-177 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s7a
Takahashi Y, Wu J, Suzuki K, Martínez Redondo P, Li M, Liao H, et al. Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells. Science. 2017;356:503-508 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig s1a
Cha Y, Han M, Cha H, Zoldan J, Burkart A, Jung J, et al. Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol. 2017;19:445-456 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 1f
Jung Klawitter S, Ebersold J, Göhring G, Blau N, Opladen T. Generation of an iPSC line from a patient with GTP cyclohydrolase 1 (GCH1) deficiency: HDMC0061i-GCH1. Stem Cell Res. 2017;20:38-41 pubmed publisher
  • immunocytochemistry; mouse; fig s2a
  • immunocytochemistry; human; fig s1d
Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell. 2017;169:243-257.e25 pubmed publisher
  • immunocytochemistry; mouse; 1:50; loading ...; fig 3c
Itakura G, Kawabata S, Ando M, Nishiyama Y, Sugai K, Ozaki M, et al. Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives. Stem Cell Reports. 2017;8:673-684 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:800; loading ...; fig st12
Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  • immunocytochemistry; human; fig s2i
Ercan E, Han J, Di Nardo A, Winden K, Han M, Hoyo L, et al. Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. J Exp Med. 2017;214:681-697 pubmed publisher
  • western blot; pigs ; loading ...; fig 2c
Genovese N, Domeier T, Telugu B, Roberts R. Enhanced Development of Skeletal Myotubes from Porcine Induced Pluripotent Stem Cells. Sci Rep. 2017;7:41833 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 4b
Wu J, Platero Luengo A, Sakurai M, Sugawara A, Gil M, Yamauchi T, et al. Interspecies Chimerism with Mammalian Pluripotent Stem Cells. Cell. 2017;168:473-486.e15 pubmed publisher
  • immunocytochemistry; mouse; fig S1b
Maltabe V, Barka E, Kontonika M, Florou D, Kouvara Pritsouli M, Roumpi M, et al. Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int. 2016;2016:8305624 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 6d
Price A, Huang E, Sebastiano V, Dunn A. A semi-interpenetrating network of polyacrylamide and recombinant basement membrane allows pluripotent cell culture in a soft, ligand-rich microenvironment. Biomaterials. 2017;121:179-192 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig s1q
Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, et al. Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 2017;18:68-81 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 7c
Dormiani K, Mir Mohammad Sadeghi H, Sadeghi Aliabadi H, Forouzanfar M, Baharvand H, Ghaedi K, et al. Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming. Cell J. 2017;18:565-581 pubmed
  • immunocytochemistry; mouse; fig 1b
Lin J, Khan M, Zapiec B, Mombaerts P. Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos. Sci Rep. 2016;6:39457 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3e
  • western blot; human; loading ...; fig 2f
Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, et al. PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. Cell Stem Cell. 2017;20:274-289.e7 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 1d
Jung Klawitter S, Blau N, Sebe A, Ebersold J, Göhring G, Opladen T. Generation of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC. Stem Cell Res. 2016;17:580-583 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s2c
Zeltner N, Fattahi F, Dubois N, Saurat N, Lafaille F, Shang L, et al. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia. Nat Med. 2016;22:1421-1427 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig s7b
Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H. Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice. PLoS Genet. 2016;12:e1006375 pubmed publisher
  • western blot; mouse; 1:5000; fig 1a
Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto A, Yang Y, Ntziachristos P, et al. Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nat Cell Biol. 2016;18:1127-1138 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1e
Zheng X, Yang P, Lackford B, Bennett B, Wang L, Li H, et al. CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State. Stem Cell Reports. 2016;7:897-910 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1
Okata S, Yuasa S, Suzuki T, Ito S, Makita N, Yoshida T, et al. Embryonic type Na+ channel ?-subunit, SCN3B masks the disease phenotype of Brugada syndrome. Sci Rep. 2016;6:34198 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1C
  • western blot; human; loading ...; fig 1E; 3F
Yoffe Y, David M, Kalaora R, Povodovski L, Friedlander G, Feldmesser E, et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev. 2016;30:1991-2004 pubmed publisher
  • immunocytochemistry; human; 1:500; fig s2
Borgs L, Peyre E, Alix P, Hanon K, Grobarczyk B, Godin J, et al. Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects. Sci Rep. 2016;6:33377 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; tbl 1
Bao X, Lian X, Palecek S. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions. Methods Mol Biol. 2016;1481:183-96 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1A
Tancos Z, Varga E, Kovacs E, Dinnyes A, Kobolak J. Establishment of induced pluripotent stem cell (iPSC) line from a 75-year old patient with late onset Alzheimer's disease (LOAD). Stem Cell Res. 2016;17:81-83 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1A
Chandrasekaran A, Varga E, Nemes C, Tancos Z, Kobolak J, Dinnyes A. Establishment of induced pluripotent stem cell (iPSC) line from a 63-year old patient with late onset Alzheimer's disease (LOAD). Stem Cell Res. 2016;17:78-80 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1A
Tancos Z, Varga E, Kovacs E, Dinnyes A, Kobolak J. Establishment of induced pluripotent stem cell (iPSC) line from an 84-year old patient with late onset Alzheimer's disease (LOAD). Stem Cell Res. 2016;17:75-77 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1A
Ochalek A, Nemes C, Varga E, Tancos Z, Kobolak J, Dinnyes A. Establishment of induced pluripotent stem cell (iPSC) line from a 57-year old patient with sporadic Alzheimer's disease. Stem Cell Res. 2016;17:72-74 pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig s1b
Hofbauer P, Jung J, McArdle T, Ogle B. Simple Monolayer Differentiation of Murine Cardiomyocytes via Nutrient Deprivation-Mediated Activation of β-Catenin. Stem Cell Rev. 2016;12:731-743 pubmed
  • immunocytochemistry; mouse; 1:100; fig 2
Martin Gonzalez J, Morgani S, Bone R, Bonderup K, Abelchian S, Brakebusch C, et al. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016;7:177-91 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; tbl 1
Vega Crespo A, Truong B, Hermann K, Awe J, Chang K, Lee P, et al. Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells. Mol Ther Methods Clin Dev. 2016;3:16050 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 2
Wang J, Liu X, Jiang Z, Li L, Cui Z, Gao Y, et al. A novel method to limit breast cancer stem cells in states of quiescence, proliferation or differentiation: Use of gel stress in combination with stem cell growth factors. Oncol Lett. 2016;12:1355-1360 pubmed
  • immunocytochemistry; mouse; loading ...; fig s5a
Itahana Y, Zhang J, Göke J, Vardy L, Han R, Iwamoto K, et al. Histone modifications and p53 binding poise the p21 promoter for activation in human embryonic stem cells. Sci Rep. 2016;6:28112 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig s1b
Desrochers L, Bordeleau F, Reinhart King C, Cerione R, Antonyak M. Microvesicles provide a mechanism for intercellular communication by embryonic stem cells during embryo implantation. Nat Commun. 2016;7:11958 pubmed publisher
  • western blot; mouse; fig 7
Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
  • flow cytometry; human; 1:50; fig 2
Tomasello L, Musso R, Cillino G, Pitrone M, Pizzolanti G, Coppola A, et al. Donor age and long-term culture do not negatively influence the stem potential of limbal fibroblast-like stem cells. Stem Cell Res Ther. 2016;7:83 pubmed publisher
  • ChIP-Seq; human; fig 4a
Tu S, Narendra V, Yamaji M, Vidal S, Rojas L, Wang X, et al. Co-repressor CBFA2T2 regulates pluripotency and germline development. Nature. 2016;534:387-90 pubmed publisher
  • immunohistochemistry; human; 1:500; loading ...; fig s9c
Hyslop L, Blakeley P, Craven L, Richardson J, Fogarty N, Fragouli E, et al. Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease. Nature. 2016;534:383-6 pubmed publisher
  • immunocytochemistry; human; fig 5
  • western blot; human; fig 5
Qi D, Wang Q, Yu M, Lan R, Li S, Lu F. Mitotic phosphorylation of SOX2 mediated by Aurora kinase A is critical for the stem-cell like cell maintenance in PA-1 cells. Cell Cycle. 2016;15:2009-18 pubmed publisher
  • western blot; human; fig 1
Jia M, Wei Z, Liu P, Zhao X. Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells. J Korean Med Sci. 2016;31:836-42 pubmed publisher
  • western blot; mouse; fig 1
Zhang W, Ni P, Mou C, Zhang Y, Guo H, Zhao T, et al. Cops2 promotes pluripotency maintenance by Stabilizing Nanog Protein and Repressing Transcription. Sci Rep. 2016;6:26804 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig s1b
Ninomiya Y, Zhao W, Saga Y. GBIQ: a non-arbitrary, non-biased method for quantification of fluorescent images. Sci Rep. 2016;6:26454 pubmed publisher
  • immunocytochemistry; human; fig 1
Reboun M, Rybová J, Dobrovolny R, Vcelak J, Veselková T, Storkanova G, et al. X-Chromosome Inactivation Analysis in Different Cell Types and Induced Pluripotent Stem Cells Elucidates the Disease Mechanism in a Rare Case of Mucopolysaccharidosis Type II in a Female. Folia Biol (Praha). 2016;62:82-9 pubmed
  • immunocytochemistry; mouse; 1:200; fig 1
Pandolfini L, Luzi E, Bressan D, Ucciferri N, Bertacchi M, Brandi R, et al. RISC-mediated control of selected chromatin regulators stabilizes ground state pluripotency of mouse embryonic stem cells. Genome Biol. 2016;17:94 pubmed publisher
  • western blot; human; fig 5
Bie Q, Sun C, Gong A, Li C, Su Z, Zheng D, et al. Non-tumor tissue derived interleukin-17B activates IL-17RB/AKT/β-catenin pathway to enhance the stemness of gastric cancer. Sci Rep. 2016;6:25447 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Chen P, Hsiao J, Sirois C, Chamberlain S. RBFOX1 and RBFOX2 are dispensable in iPSCs and iPSC-derived neurons and do not contribute to neural-specific paternal UBE3A silencing. Sci Rep. 2016;6:25368 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 2b
Shahbazi M, Jedrusik A, Vuoristo S, Recher G, Hupalowska A, Bolton V, et al. Self-organization of the human embryo in the absence of maternal tissues. Nat Cell Biol. 2016;18:700-708 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 5
  • western blot; human; fig 5
Kushwaha R, Jagadish N, Kustagi M, Mendiratta G, Seandel M, Soni R, et al. Mechanism and Role of SOX2 Repression in Seminoma: Relevance to Human Germline Specification. Stem Cell Reports. 2016;6:772-783 pubmed publisher
  • flow cytometry; human; fig s1
Li D, Wang L, Hou J, Shen Q, Chen Q, Wang X, et al. Optimized Approaches for Generation of Integration-free iPSCs from Human Urine-Derived Cells with Small Molecules and Autologous Feeder. Stem Cell Reports. 2016;6:717-728 pubmed publisher
  • immunocytochemistry; mouse; 1:250; loading ...; fig s3c
Sharova L, Sharov A, Piao Y, Stagg C, Amano T, Qian Y, et al. Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment. In Vitro Cell Dev Biol Anim. 2016;52:616-24 pubmed publisher
  • immunohistochemistry - frozen section; African green monkey; 1:400; fig 2
Seita Y, Tsukiyama T, Iwatani C, Tsuchiya H, Matsushita J, Azami T, et al. Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body. Sci Rep. 2016;6:24868 pubmed publisher
  • immunocytochemistry; human; 1:200; fig s1
Burridge P, Li Y, Matsa E, Wu H, Ong S, Sharma A, et al. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the predilection of breast cancer patients to doxorubicin-induced cardiotoxicity. Nat Med. 2016;22:547-56 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 2
Sadeghian Nodoushan F, Aflatoonian R, Borzouie Z, Akyash F, Fesahat F, Soleimani M, et al. Pluripotency and differentiation of cells from human testicular sperm extraction: An investigation of cell stemness. Mol Reprod Dev. 2016;83:312-23 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 1
Conway A, Van Nostrand E, Pratt G, Aigner S, Wilbert M, Sundararaman B, et al. Enhanced CLIP Uncovers IMP Protein-RNA Targets in Human Pluripotent Stem Cells Important for Cell Adhesion and Survival. Cell Rep. 2016;15:666-679 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2g
Saito H, Okita K, Fusaki N, Sabel M, Chang A, Ito F. Reprogramming of Melanoma Tumor-Infiltrating Lymphocytes to Induced Pluripotent Stem Cells. Stem Cells Int. 2016;2016:8394960 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 2
Sa Ngiamsuntorn K, Wongkajornsilp A, Phanthong P, Borwornpinyo S, Kitiyanant N, Chantratita W, et al. A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host. Virol J. 2016;13:59 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3a
  • western blot; mouse; 1:1000; fig 1d
Navarra A, Musto A, Gargiulo A, Petrosino G, Pierantoni G, Fusco A, et al. Hmga2 is necessary for Otx2-dependent exit of embryonic stem cells from the pluripotent ground state. BMC Biol. 2016;14:24 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 3a
Meng G, Poon A, Liu S, Rancourt D. An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells. Methods Mol Biol. 2016;1516:47-56 pubmed publisher
  • immunocytochemistry; human; fig s3a
  • western blot; human; fig s3b
Vicidomini C, Ponzoni L, Lim D, Schmeisser M, Reim D, Morello N, et al. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Mol Psychiatry. 2017;22:689-702 pubmed publisher
  • western blot; human; fig 7
Ananthula S, Sinha A, El Gassim M, Batth S, Marshall G, Gardner L, et al. Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers. Oncotarget. 2016;7:20869-89 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 8
Ghosheh N, Olsson B, Edsbagge J, Küppers Munther B, van Giezen M, Asplund A, et al. Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines. Stem Cells Int. 2016;2016:8648356 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
  • western blot; human; 1:1000; fig 1
Chang T, Chen C, Wu Y, Liu J, Kuo Y, Lee K, et al. Inflammation Promotes Expression of Stemness-Related Properties in HBV-Related Hepatocellular Carcinoma. PLoS ONE. 2016;11:e0149897 pubmed publisher
  • immunocytochemistry; human; 1:500
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
  • immunohistochemistry; human; fig 3
  • western blot; human; fig 3
Castaño J, Morera C, Sesé B, Boue S, Bonet Costa C, Marti M, et al. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. PLoS ONE. 2016;11:e0149502 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 3b
Kang L, Yao C, Khodadadi Jamayran A, Xu W, Zhang R, Banerjee N, et al. The Universal 3D3 Antibody of Human PODXL Is Pluripotent Cytotoxic, and Identifies a Residual Population After Extended Differentiation of Pluripotent Stem Cells. Stem Cells Dev. 2016;25:556-68 pubmed publisher
  • chromatin immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 2
Morales Hernández A, González Rico F, Román A, Rico Leo E, Alvarez Barrientos A, Sánchez L, et al. Alu retrotransposons promote differentiation of human carcinoma cells through the aryl hydrocarbon receptor. Nucleic Acids Res. 2016;44:4665-83 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 1a
  • western blot; mouse; fig 1b
Shin J, Kim T, Kim H, Kim H, Suh M, Lee S, et al. Aurkb/PP1-mediated resetting of Oct4 during the cell cycle determines the identity of embryonic stem cells. elife. 2016;5:e10877 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1a
Li Q, Lex R, Chung H, Giovanetti S, Ji Z, Ji H, et al. The Pluripotency Factor NANOG Binds to GLI Proteins and Represses Hedgehog-mediated Transcription. J Biol Chem. 2016;291:7171-82 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • immunocytochemistry; human; 1:100; fig s1
Pan H, Guan D, Liu X, Li J, Wang L, Wu J, et al. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016;26:190-205 pubmed publisher
  • western blot; human; 1:750; fig 1
Klawitter S, Fuchs N, Upton K, Muñoz Lopez M, Shukla R, Wang J, et al. Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells. Nat Commun. 2016;7:10286 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 5s1a
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
  • immunocytochemistry; human; fig 6
Higuchi A, Kao S, Ling Q, Chen Y, Li H, Alarfaj A, et al. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep. 2015;5:18136 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 2b
Ye S, Zhang D, Cheng F, Wilson D, Mackay J, He K, et al. Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal. J Cell Sci. 2016;129:269-76 pubmed publisher
  • western blot; human; fig 4a
Wongtrakoongate P, Riddick G, Fucharoen S, Felsenfeld G. Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes. PLoS Genet. 2015;11:e1005615 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1
Freedman B, Brooks C, Lam A, Fu H, Morizane R, Agrawal V, et al. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Commun. 2015;6:8715 pubmed publisher
  • western blot; human; fig 1
Lee J, Lee S, Heo S, Kim K, Kim C, Kim D, et al. Novel Function of Lysine Methyltransferase G9a in the Regulation of Sox2 Protein Stability. PLoS ONE. 2015;10:e0141118 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1
Epsztejn Litman S, Cohen Hadad Y, Aharoni S, Altarescu G, Renbaum P, Levy Lahad E, et al. Establishment of Homozygote Mutant Human Embryonic Stem Cells by Parthenogenesis. PLoS ONE. 2015;10:e0138893 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 3
  • immunocytochemistry; human; fig 3
Donzelli S, Mori F, Bellissimo T, Sacconi A, Casini B, Frixa T, et al. Epigenetic silencing of miR-145-5p contributes to brain metastasis. Oncotarget. 2015;6:35183-201 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 6
Straccia M, Garcia Díaz Barriga G, Sanders P, Bombau G, Carrere J, Mairal P, et al. Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell-derived medium spiny neurons. Mol Ther Methods Clin Dev. 2015;2:15030 pubmed publisher
  • immunocytochemistry; human; fig 4
Agu C, Soares F, Alderton A, Patel M, Ansari R, Patel S, et al. Successful Generation of Human Induced Pluripotent Stem Cell Lines from Blood Samples Held at Room Temperature for up to 48 hr. Stem Cell Reports. 2015;5:660-71 pubmed publisher
  • immunocytochemistry; human; 5 ug/ml; loading ...; fig 3d
Hung S, Pébay A, Wong R. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J Vis Exp. 2015;:e53174 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:50; loading ...; fig 4c
Runesson E, Ackermann P, Karlsson J, Eriksson B. Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing. BMC Musculoskelet Disord. 2015;16:212 pubmed publisher
  • immunocytochemistry; bovine; 1:25; fig 3
Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, et al. Generation of Naïve Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors. PLoS ONE. 2015;10:e0135403 pubmed publisher
  • immunocytochemistry; human; 1:300; fig 2
Ishikawa M, Ohnishi H, Skerleva D, Sakamoto T, Yamamoto N, Hotta A, et al. Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae. J Tissue Eng Regen Med. 2017;11:1766-1778 pubmed publisher
  • immunocytochemistry; rat; 1:100
Mohammadi A, Attari F, Babapour V, Hassani S, Masoudi N, Shahverdi A, et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J. 2015;17:288-95 pubmed
  • immunocytochemistry; human; 1:100; fig 1
Moslem M, Eberle I, Weber I, Henschler R, Cantz T. Mesenchymal Stem/Stromal Cells Derived from Induced Pluripotent Stem Cells Support CD34(pos) Hematopoietic Stem Cell Propagation and Suppress Inflammatory Reaction. Stem Cells Int. 2015;2015:843058 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 7
Xu J, Wan P, Wang M, Zhang J, Gao X, Hu B, et al. AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment. Cell Death Dis. 2015;6:e1818 pubmed publisher
  • immunocytochemistry; human; 1:100; fig st1
Jones A, Gokhale P, Allison T, Sampson B, Athwal S, Grant S, et al. Evidence for bystander signalling between human trophoblast cells and human embryonic stem cells. Sci Rep. 2015;5:11694 pubmed publisher
  • immunocytochemistry; mouse; 1:250; fig 1
O Shaughnessy Kirwan A, Signolet J, Costello I, Gharbi S, Hendrich B. Constraint of gene expression by the chromatin remodelling protein CHD4 facilitates lineage specification. Development. 2015;142:2586-97 pubmed publisher
  • immunocytochemistry; African green monkey; 1:100; fig 1s2
Gallego Romero I, Pavlovic B, Hernando Herraez I, Zhou X, WARD M, Banovich N, et al. A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. elife. 2015;4:e07103 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig 6
  • western blot; mouse; 1:1000; fig 6
Liu J, Han Q, Peng T, Peng M, Wei B, Li D, et al. The oncogene c-Jun impedes somatic cell reprogramming. Nat Cell Biol. 2015;17:856-67 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 3
Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, et al. Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Nat Biotechnol. 2015;33:769-74 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 1d
  • western blot; mouse; 1:1000
Szlachcic W, Switonski P, Krzyzosiak W, Figlerowicz M, Figiel M. Huntington disease iPSCs show early molecular changes in intracellular signaling, the expression of oxidative stress proteins and the p53 pathway. Dis Model Mech. 2015;8:1047-57 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
  • western blot; human
Huang X, Hu Q, Braun G, Pallaoro A, Morales D, ZASADZINSKI J, et al. Light-activated RNA interference in human embryonic stem cells. Biomaterials. 2015;63:70-9 pubmed publisher
  • flow cytometry; mouse; 1:50
Vrbsky J, Tereh T, Kyrylenko S, Dvorak P, Krejci L. MEK and TGF-beta Inhibition Promotes Reprogramming without the Use of Transcription Factor. PLoS ONE. 2015;10:e0127739 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 3d
Higuchi Y, Nguyen C, Yasuda S, McMillan M, Hasegawa K, Kahn M. Specific Direct Small Molecule p300/?-Catenin Antagonists Maintain Stem Cell Potency. Curr Mol Pharmacol. 2016;9:272-279 pubmed
  • immunocytochemistry; mouse; 1:200; fig 1
  • immunocytochemistry; human; 1:200; fig 5
  • immunocytochemistry; rhesus macaque; 1:200; fig s10
Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, et al. An alternative pluripotent state confers interspecies chimaeric competency. Nature. 2015;521:316-21 pubmed publisher
  • immunoprecipitation; mouse; fig 3
Kim T, Kang B, Jang H, Kwak S, Shin J, Kim H, et al. Ctbp2 Modulates NuRD-Mediated Deacetylation of H3K27 and Facilitates PRC2-Mediated H3K27me3 in Active Embryonic Stem Cell Genes During Exit from Pluripotency. Stem Cells. 2015;33:2442-55 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 1
Jung K, Gupta N, Wang P, Lewis J, Gopal K, Wu F, et al. Triple negative breast cancers comprise a highly tumorigenic cell subpopulation detectable by its high responsiveness to a Sox2 regulatory region 2 (SRR2) reporter. Oncotarget. 2015;6:10366-73 pubmed
  • immunohistochemistry; mouse; 1:1000; fig 2
Adams K, Rousso D, Umbach J, Novitch B. Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells. Nat Commun. 2015;6:6778 pubmed publisher
  • immunocytochemistry; human; 1:500; fig s2
Sun Y, Florer J, Mayhew C, Jia Z, Zhao Z, Xu K, et al. Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology. PLoS ONE. 2015;10:e0118771 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig s2
  • immunocytochemistry; human; fig s2
Li S, Wu X, Dong C, Xie X, Wu J, Zhang X. The differential expression of OCT4 isoforms in cervical carcinoma. PLoS ONE. 2015;10:e0118033 pubmed publisher
  • western blot; mouse; fig 1
Lu W, Fang L, Ouyang B, Zhang X, Zhan S, Feng X, et al. Actl6a protects embryonic stem cells from differentiating into primitive endoderm. Stem Cells. 2015;33:1782-93 pubmed publisher
  • flow cytometry; human; 1:100; fig 1c
Sugawa F, Araúzo Bravo M, Yoon J, Kim K, Aramaki S, Wu G, et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J. 2015;34:1009-24 pubmed publisher
  • immunocytochemistry; mouse; fig 2
Nukaya D, Minami K, Hoshikawa R, Yokoi N, Seino S. Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas. Genes Cells. 2015;20:367-81 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s5a
Nakamura T, Yabuta Y, Okamoto I, Aramaki S, Yokobayashi S, Kurimoto K, et al. SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression. Nucleic Acids Res. 2015;43:e60 pubmed publisher
  • western blot; mouse
Yan H, Solozobova V, Zhang P, Armant O, Kuehl B, Brenner Weiss G, et al. p53 is active in murine stem cells and alters the transcriptome in a manner that is reminiscent of mutant p53. Cell Death Dis. 2015;6:e1662 pubmed publisher
  • western blot; human; fig 5
Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El Sayed K, et al. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-β. PLoS ONE. 2015;10:e0118097 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Liskovykh M, Ponomartsev S, Popova E, Bader M, Kouprina N, Larionov V, et al. Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice. Cell Cycle. 2015;14:1268-73 pubmed publisher
  • immunocytochemistry; human; 1:100
Fritz A, Adil M, Mao S, Schaffer D. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming. Mol Ther. 2015;23:952-963 pubmed publisher
  • western blot; human; fig 5
Rao R, Dhele N, Cheemadan S, Ketkar A, Jayandharan G, Palakodeti D, et al. Ezh2 mediated H3K27me3 activity facilitates somatic transition during human pluripotent reprogramming. Sci Rep. 2015;5:8229 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 5c
  • immunocytochemistry; mouse; 1:100; fig 5a
Choi S, Lee H, Choi J, Kim J, Park C, Joo H, et al. Cyclosporin A induces cardiac differentiation but inhibits hemato-endothelial differentiation of P19 cells. PLoS ONE. 2015;10:e0117410 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 3
Piatti P, Lim C, Nat R, Villunger A, Geley S, Shue Y, et al. Embryonic stem cell differentiation requires full length Chd1. Sci Rep. 2015;5:8007 pubmed publisher
  • western blot; rat
Ndisang J, Tiwari S. Mechanisms by which heme oxygenase rescue renal dysfunction in obesity. Redox Biol. 2014;2:1029-37 pubmed publisher
  • immunocytochemistry; human; 1:300
Wilson P, Payne T. Genetic reprogramming of human amniotic cells with episomal vectors: neural rosettes as sentinels in candidate selection for validation assays. Peerj. 2014;2:e668 pubmed publisher
  • immunohistochemistry; human; 1:200; loading ...; fig 3a
  • western blot; human; 1:1000; loading ...; fig 3c
Abu Hassan D, Li X, Ryan E, Acott T, Kelley M. Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma. Stem Cells. 2015;33:751-61 pubmed publisher
  • immunohistochemistry; mouse
Wainwright E, Svingen T, Ng E, Wicking C, Koopman P. Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice. Dev Biol. 2014;395:342-54 pubmed publisher
  • immunocytochemistry; rat; fig 2
Chau M, Deveau T, Song M, Gu X, Chen D, Wei L. iPSC Transplantation increases regeneration and functional recovery after ischemic stroke in neonatal rats. Stem Cells. 2014;32:3075-87 pubmed publisher
  • immunocytochemistry; human; 1:75
Ovchinnikov D, Titmarsh D, Fortuna P, Hidalgo A, Alharbi S, Whitworth D, et al. Transgenic human ES and iPS reporter cell lines for identification and selection of pluripotent stem cells in vitro. Stem Cell Res. 2014;13:251-61 pubmed publisher
  • immunohistochemistry; human; 1:100
Mosbech C, Svingen T, Nielsen J, Toft B, Rechnitzer C, Petersen B, et al. Expression pattern of clinically relevant markers in paediatric germ cell- and sex-cord stromal tumours is similar to adult testicular tumours. Virchows Arch. 2014;465:567-77 pubmed publisher
  • western blot; mouse
Ueda J, Ho J, Lee K, Kitajima S, Yang H, Sun W, et al. The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth. Mol Cell Biol. 2014;34:3702-20 pubmed publisher
  • immunocytochemistry; human
Fritz A, Mao S, West M, Schaffer D. A medium-throughput analysis of signaling pathways involved in early stages of stem cell reprogramming. Biotechnol Bioeng. 2015;112:209-19 pubmed publisher
  • western blot; human; 1:500
Tan G, Chan E, Molnar A, Sarkar R, Alexieva D, Isa I, et al. 5' isomiR variation is of functional and evolutionary importance. Nucleic Acids Res. 2014;42:9424-35 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1
Liu G, Suzuki K, Li M, Qu J, Montserrat N, Tarantino C, et al. Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs. Nat Commun. 2014;5:4330 pubmed publisher
  • immunocytochemistry; mouse; 1:100
Hassani S, Pakzad M, Asgari B, Taei A, Baharvand H. Suppression of transforming growth factor ? signaling promotes ground state pluripotency from single blastomeres. Hum Reprod. 2014;29:1739-48 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 3a
Soltanian S, Dehghani H, Matin M, Bahrami A. Expression analysis of BORIS during pluripotent, differentiated, cancerous, and non-cancerous cell states. Acta Biochim Biophys Sin (Shanghai). 2014;46:647-58 pubmed publisher
  • EMSA; human; fig 4
Qian X, Zhao F. Collaborative interaction of Oct-2 with Oct-1 in transactivation of lactogenic hormones-induced ?-casein gene expression in mammary epithelial cells. Gen Comp Endocrinol. 2014;204:185-94 pubmed publisher
  • western blot; mouse; fig 1b
Chelmicki T, Dündar F, Turley M, Khanam T, Aktas T, Ramirez F, et al. MOF-associated complexes ensure stem cell identity and Xist repression. elife. 2014;3:e02024 pubmed publisher
  • immunocytochemistry; mouse; 1:500; loading ...; fig 3b
Li T, Yang D, Li J, Tang Y, Yang J, Le W. Critical role of Tet3 in neural progenitor cell maintenance and terminal differentiation. Mol Neurobiol. 2015;51:142-54 pubmed publisher
  • immunocytochemistry; human; 1:250
Krutá M, Šeneklová M, Raška J, Salykin A, Zerzankova L, Pesl M, et al. Mutation frequency dynamics in HPRT locus in culture-adapted human embryonic stem cells and induced pluripotent stem cells correspond to their differentiated counterparts. Stem Cells Dev. 2014;23:2443-54 pubmed publisher
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Seki M, Masaki H, Arauchi T, Nakauchi H, Sugano S, Suzuki Y. A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells. PLoS ONE. 2014;9:e95374 pubmed publisher
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Svingen T, Jørgensen A, Rajpert De Meyts E. Validation of endogenous normalizing genes for expression analyses in adult human testis and germ cell neoplasms. Mol Hum Reprod. 2014;20:709-18 pubmed publisher
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Kanemura H, Go M, Shikamura M, Nishishita N, Sakai N, Kamao H, et al. Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration. PLoS ONE. 2014;9:e85336 pubmed publisher
  • In-Cell Western; dogs; 1:200; fig 4
Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, et al. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med. 2014;3:334-45 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 2
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Izumikawa T, Sato B, Kitagawa H. Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells. Sci Rep. 2014;4:3701 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 1
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Abdelalim E, Tooyama I. Knockdown of p53 suppresses Nanog expression in embryonic stem cells. Biochem Biophys Res Commun. 2014;443:652-7 pubmed publisher
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  • western blot; mouse
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product information
Product Name :
Oct-3/4 (C-10) Antibody
Gene Name :
POU5F1
Size :
200 µg/ml
Catalog Number :
sc-5279
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Oct-3/4
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
1-134 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA