product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Lck (3A5) Antibody
catalog :
sc-433
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3A5
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 40
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 6a
Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley L, et al. Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol. 2018;19:594-605 pubmed publisher
  • western blot; human; loading ...; fig s7
Li L, Guo X, Shi X, Li C, Wu W, Yan C, et al. Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling. Proc Natl Acad Sci U S A. 2017;114:E5891-E5899 pubmed publisher
  • western blot; human; loading ...; fig 3c
Chattopadhyay R, Raghavan S, Rao G. Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function. Redox Biol. 2017;12:438-455 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1a
Silva J, Martins N, Henriques R, Soares H. HIV-1 Nef Impairs the Formation of Calcium Membrane Territories Controlling the Signaling Nanoarchitecture at the Immunological Synapse. J Immunol. 2016;197:4042-4052 pubmed
  • flow cytometry; human; fig 1c
Trautz B, Pierini V, Wombacher R, Stolp B, Chase A, Pizzato M, et al. The Antagonism of HIV-1 Nef to SERINC5 Particle Infectivity Restriction Involves the Counteraction of Virion-Associated Pools of the Restriction Factor. J Virol. 2016;90:10915-10927 pubmed publisher
  • immunocytochemistry; human; fig 4
Pageon S, Tabarin T, Yamamoto Y, Ma Y, Nicovich P, Bridgeman J, et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. Proc Natl Acad Sci U S A. 2016;113:E5454-63 pubmed publisher
  • western blot; human; loading ...; fig 3a
Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, et al. Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?. PLoS ONE. 2016;11:e0154737 pubmed publisher
  • western blot; mouse; fig 5
Hu H, Wang H, Xiao Y, Jin J, Chang J, Zou Q, et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med. 2016;213:399-414 pubmed publisher
  • western blot; mouse; fig 6
Franciosa G, Diluvio G, Gaudio F, Giuli M, Palermo R, Grazioli P, et al. Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression. Oncogene. 2016;35:4741-51 pubmed publisher
  • western blot; mouse; fig s4
De Mario A, Castellani A, Peggion C, Massimino M, Lim D, Hill A, et al. The prion protein constitutively controls neuronal store-operated Ca(2+) entry through Fyn kinase. Front Cell Neurosci. 2015;9:416 pubmed publisher
  • western blot; mouse; fig 6a
Zou Q, Jin J, Xiao Y, Hu H, Zhou X, Jie Z, et al. T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. J Exp Med. 2015;212:1323-36 pubmed publisher
  • western blot; mouse; fig 1
Silva O, Crocetti J, Humphries L, Burkhardt J, Miceli M. Discs Large Homolog 1 Splice Variants Regulate p38-Dependent and -Independent Effector Functions in CD8+ T Cells. PLoS ONE. 2015;10:e0133353 pubmed publisher
  • western blot; human; loading ...; fig 1c
Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks S, Gunnarsson J, et al. MALT1 auto-proteolysis is essential for NF-κB-dependent gene transcription in activated lymphocytes. PLoS ONE. 2014;9:e103774 pubmed publisher
  • western blot; human; fig 3
Sakamoto T, Kobayashi M, Tada K, Shinohara M, Io K, Nagata K, et al. CKIP-1 is an intrinsic negative regulator of T-cell activation through an interaction with CARMA1. PLoS ONE. 2014;9:e85762 pubmed publisher
  • western blot; human
Kloog Y, Mor A. Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1. Mol Cell Biol. 2014;34:978-88 pubmed publisher
  • western blot; human
Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez Riaño A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
  • western blot; human; fig 1
Ngai J, Inngjerdingen M, Berge T, Tasken K. Interplay between the heterotrimeric G-protein subunits Galphaq and Galphai2 sets the threshold for chemotaxis and TCR activation. BMC Immunol. 2009;10:27 pubmed publisher
Rajasekaran K, Kumar P, Schuldt K, Peterson E, Vanhaesebroeck B, Dixit V, et al. Signaling by Fyn-ADAP via the Carma1-Bcl-10-MAP3K7 signalosome exclusively regulates inflammatory cytokine production in NK cells. Nat Immunol. 2013;14:1127-36 pubmed publisher
Gupta R, Palchaudhuri S, Chattopadhyay D. Glutamate induces neutrophil cell migration by activating class I metabotropic glutamate receptors. Amino Acids. 2013;44:757-67 pubmed publisher
Scales T, Derkinderen P, Leung K, Byers H, Ward M, Price C, et al. Tyrosine phosphorylation of tau by the SRC family kinases lck and fyn. Mol Neurodegener. 2011;6:12 pubmed publisher
Harr M, McColl K, Zhong F, Molitoris J, Distelhorst C. Glucocorticoids downregulate Fyn and inhibit IP(3)-mediated calcium signaling to promote autophagy in T lymphocytes. Autophagy. 2010;6:912-21 pubmed publisher
Thien C, Dagger S, Steer J, Koentgen F, Jansen E, Scott C, et al. c-Cbl promotes T cell receptor-induced thymocyte apoptosis by activating the phosphatidylinositol 3-kinase/Akt pathway. J Biol Chem. 2010;285:10969-81 pubmed publisher
Hollmann C, Tzankov A, Martinez Marignac V, Baker K, Grygorczyk C, Grygorczyk R, et al. Therapeutic implications of Src independent calcium mobilization in diffuse large B-cell lymphoma. Leuk Res. 2010;34:585-93 pubmed publisher
Ngai J, Methi T, Andressen K, Levy F, Torgersen K, Vang T, et al. The heterotrimeric G-protein alpha-subunit Galphaq regulates TCR-mediated immune responses through an Lck-dependent pathway. Eur J Immunol. 2008;38:3208-18 pubmed publisher
Emaduddin M, Edelmann M, Kessler B, Feller S. Odin (ANKS1A) is a Src family kinase target in colorectal cancer cells. Cell Commun Signal. 2008;6:7 pubmed publisher
Jennings B, Nadolski M, Ling Y, Baker M, Harrison M, Deschenes R, et al. 2-Bromopalmitate and 2-(2-hydroxy-5-nitro-benzylidene)-benzo[b]thiophen-3-one inhibit DHHC-mediated palmitoylation in vitro. J Lipid Res. 2009;50:233-42 pubmed publisher
Methi T, Berge T, Torgersen K, Tasken K. Reduced Cbl phosphorylation and degradation of the zeta-chain of the T-cell receptor/CD3 complex in T cells with low Lck levels. Eur J Immunol. 2008;38:2557-63 pubmed publisher
Naito M, Komohara Y, Ishihara Y, Noguchi M, Yamashita Y, Shirakusa T, et al. Identification of Lck-derived peptides applicable to anti-cancer vaccine for patients with human leukocyte antigen-A3 supertype alleles. Br J Cancer. 2007;97:1648-54 pubmed
Methi T, Ngai J, Vang T, Torgersen K, Tasken K. Hypophosphorylated TCR/CD3zeta signals through a Grb2-SOS1-Ras pathway in Lck knockdown cells. Eur J Immunol. 2007;37:2539-48 pubmed
Bunnell S, Singer A, Hong D, Jacque B, Jordan M, Seminario M, et al. Persistence of cooperatively stabilized signaling clusters drives T-cell activation. Mol Cell Biol. 2006;26:7155-66 pubmed
Nagy G, Ward J, Mosser D, Koncz A, Gergely P, Stancato C, et al. Regulation of CD4 expression via recycling by HRES-1/RAB4 controls susceptibility to HIV infection. J Biol Chem. 2006;281:34574-91 pubmed
Lee M, Beauchamp R, Beyer K, Gusella J, Ramesh V. Magicin associates with the Src-family kinases and is phosphorylated upon CD3 stimulation. Biochem Biophys Res Commun. 2006;348:826-31 pubmed
Kinoshita D, Hirota F, Kaisho T, Kasai M, Izumi K, Bando Y, et al. Essential role of IkappaB kinase alpha in thymic organogenesis required for the establishment of self-tolerance. J Immunol. 2006;176:3995-4002 pubmed
Marti F, Garcia G, Lapinski P, Macgregor J, King P. Essential role of the T cell-specific adapter protein in the activation of LCK in peripheral T cells. J Exp Med. 2006;203:281-7 pubmed
Methi T, Ngai J, Mahic M, Amarzguioui M, Vang T, Tasken K. Short-interfering RNA-mediated Lck knockdown results in augmented downstream T cell responses. J Immunol. 2005;175:7398-406 pubmed
Lygren B, Tasken K, Carlson C. A fast and sensitive method for isolation of detergent-resistant membranes from T cells. J Immunol Methods. 2005;305:199-205 pubmed
Zhao Y, Ramakrishnan A, Kim K, Rabson A. Regulation of Bcl-3 through interaction with the Lck tyrosine kinase. Biochem Biophys Res Commun. 2005;335:865-73 pubmed
Landsverk H, Kirkhus M, Bollen M, Küntziger T, Collas P. PNUTS enhances in vitro chromosome decondensation in a PP1-dependent manner. Biochem J. 2005;390:709-17 pubmed
Felli M, Vacca A, Calce A, Bellavia D, Campese A, Grillo R, et al. PKC theta mediates pre-TCR signaling and contributes to Notch3-induced T-cell leukemia. Oncogene. 2005;24:992-1000 pubmed
Panigada M, Porcellini S, Barbier E, Hoeflinger S, Cazenave P, Gu H, et al. Constitutive endocytosis and degradation of the pre-T cell receptor. J Exp Med. 2002;195:1585-97 pubmed
product information
Product Name :
Lck (3A5) Antibody
Gene Name :
LCK
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-433
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Lck
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM
Epitope :
1-225 (m)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA