This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
IkappaB-alpha (C-21)
catalog :
sc-371
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry
citations: 78
Published Application/Species/Sample/DilutionReference
  • western blot; human
Korsensky L, Haif S, Heller R, Rabinovitz S, Haddad Halloun J, Dahan N, et al. The tumor suppressor Sef is a scaffold for the classical NF-κB/RELA:P50 signaling module. Cell Signal. 2019;59:110-121 pubmed publisher
  • western blot; human; loading ...; fig 1c
Qiang L, Wang J, Zhang Y, Ge P, Chai Q, Li B, et al. Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation. Cell Mol Immunol. 2018;: pubmed publisher
  • western blot; rat; 1:1000; loading ...; fig 3a
Gugliandolo E, Fusco R, D Amico R, Militi A, Oteri G, Wallace J, et al. Anti-inflammatory effect of ATB-352, a H2S -releasing ketoprofen derivative, on lipopolysaccharide-induced periodontitis in rats. Pharmacol Res. 2018;132:220-231 pubmed publisher
  • western blot; human; loading ...; fig 8c
Pryke K, Abraham J, Sali T, Gall B, Archer I, Liu A, et al. A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017;8: pubmed publisher
  • western blot; mouse; loading ...; fig 5b
Shin C, Ito Y, Ichikawa S, Tokunaga M, Sakata Sogawa K, Tanaka T. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. Sci Rep. 2017;7:46097 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 6a
Paterniti I, Campolo M, Siracusa R, Cordaro M, Di Paola R, Calabrese V, et al. Liver X receptors activation, through TO901317 binding, reduces neuroinflammation in Parkinson's disease. PLoS ONE. 2017;12:e0174470 pubmed publisher
  • western blot; mouse; loading ...; fig 4c
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  • western blot; human; fig 5a
Gatla H, Zou Y, Uddin M, Singha B, Bu P, Vancura A, et al. Histone Deacetylase (HDAC) Inhibition Induces IκB Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells. J Biol Chem. 2017;292:5043-5054 pubmed publisher
  • western blot; mouse; loading ...; fig 3b
Zhang H, Wang W, Ren L, Zhao X, Wang Z, Zhuang D, et al. The mTORC2/Akt/NFκB Pathway-Mediated Activation of TRPC6 Participates in Adriamycin-Induced Podocyte Apoptosis. Cell Physiol Biochem. 2016;40:1079-1093 pubmed
  • western blot; mouse; loading ...; fig 2a
Huh H, Ra E, Lee T, Kang S, Park A, Lee E, et al. STRAP Acts as a Scaffolding Protein in Controlling the TLR2/4 Signaling Pathway. Sci Rep. 2016;6:38849 pubmed publisher
  • western blot; human; loading ...; fig 2b
Lim S, Hwang S, Yu J, Lim J, Kim H. Lycopene inhibits regulator of calcineurin 1-mediated apoptosis by reducing oxidative stress and down-regulating Nucling in neuronal cells. Mol Nutr Food Res. 2017;61: pubmed publisher
  • western blot; mouse; loading ...; fig 6c
Filliol A, Piquet Pellorce C, Le Seyec J, Farooq M, Genet V, Lucas Clerc C, et al. RIPK1 protects from TNF-α-mediated liver damage during hepatitis. Cell Death Dis. 2016;7:e2462 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 7a
Okabe T, Chavan R, Fonseca Costa S, Brenna A, Ripperger J, Albrecht U. REV-ERB? influences the stability and nuclear localization of the glucocorticoid receptor. J Cell Sci. 2016;129:4143-4154 pubmed
  • immunohistochemistry; human; 1:500; loading ...; fig 2d
  • western blot; human; loading ...; fig 1c
Wurster K, Hummel F, Richter J, Giefing M, Hartmann S, Hansmann M, et al. Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma. Leukemia. 2017;31:602-613 pubmed publisher
  • western blot; human; loading ...; fig 4a
Radke D, Ungefroren H, Helm O, Voigt S, Alp G, Braun H, et al. Negative control of TRAIL-R1 signaling by transforming growth factor ?1 in pancreatic tumor cells involves Smad-dependent down regulation of TRAIL-R1. Cell Signal. 2016;28:1652-62 pubmed publisher
  • western blot; mouse; loading ...; fig 1b
Lesina M, Wörmann S, Morton J, Diakopoulos K, Korneeva O, Wimmer M, et al. RelA regulates CXCL1/CXCR2-dependent oncogene-induced senescence in murine Kras-driven pancreatic carcinogenesis. J Clin Invest. 2016;126:2919-32 pubmed publisher
  • western blot; mouse; loading ...; fig 2a
Kang J, Park S, Jeong S, Han M, Lee C, Lee K, et al. Epigenetic regulation of Kcna3-encoding Kv1.3 potassium channel by cereblon contributes to regulation of CD4+ T-cell activation. Proc Natl Acad Sci U S A. 2016;113:8771-6 pubmed publisher
  • western blot; human; loading ...; fig 1b
Kwon H, Choi G, Ryu S, Kwon S, Kim S, Booth C, et al. Stepwise phosphorylation of p65 promotes NF-?B activation and NK cell responses during target cell recognition. Nat Commun. 2016;7:11686 pubmed publisher
  • western blot; human; loading ...; fig 7
Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed publisher
  • western blot; mouse; fig 6a
Bugajev V, Hálová I, Dráberová L, Bambousková M, Potůčková L, Draberova H, et al. Negative regulatory roles of ORMDL3 in the FcεRI-triggered expression of proinflammatory mediators and chemotactic response in murine mast cells. Cell Mol Life Sci. 2016;73:1265-85 pubmed publisher
  • western blot; mouse; fig 1
Zhang J, Li L, Baldwin A, Friedman A, Paz Priel I. Loss of IKKβ but Not NF-κB p65 Skews Differentiation towards Myeloid over Erythroid Commitment and Increases Myeloid Progenitor Self-Renewal and Functional Long-Term Hematopoietic Stem Cells. PLoS ONE. 2015;10:e0130441 pubmed publisher
Schmitz M, Dreute J, Pfisterer M, Günther S, Kracht M, Chillappagari S. SIAH ubiquitin E3 ligases as modulators of inflammatory gene expression. Heliyon. 2022;8:e09029 pubmed publisher
Oleszycka E, Rodgers A, Xu L, Moynagh P. Dendritic Cell-Specific Role for Pellino2 as a Mediator of TLR9 Signaling Pathway. J Immunol. 2021;207:2325-2336 pubmed publisher
Lee S, Ha J, Park J, Kang E, Jeon S, Han S, et al. Antioxidant and Anti-Inflammatory Effects of Bischofia javanica (Blume) Leaf Methanol Extracts through the Regulation of Nrf2 and TAK1. Antioxidants (Basel). 2021;10: pubmed publisher
Yasuda K, Matsubara T, Shirakawa T, Kawamoto T, Kokabu S. Protein phosphatase 1 regulatory subunit 18 suppresses the transcriptional activity of NFATc1 via regulation of c-fos. Bone Rep. 2021;15:101114 pubmed publisher
Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
Palomer X, Román Azcona M, Pizarro Delgado J, Planavila A, Villarroya F, Valenzuela Alcaraz B, et al. SIRT3-mediated inhibition of FOS through histone H3 deacetylation prevents cardiac fibrosis and inflammation. Signal Transduct Target Ther. 2020;5:14 pubmed publisher
Zhao J, Zhang L, Lu A, Han Y, Colangelo D, Bukata C, et al. ATM is a key driver of NF-κB-dependent DNA-damage-induced senescence, stem cell dysfunction and aging. Aging (Albany NY). 2020;12:4688-4710 pubmed publisher
Park Y, Lee H, Lim J, Kim H. Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells. Antioxidants (Basel). 2019;8: pubmed publisher
DeSisto J, Flannery P, Lemma R, Pathak A, Mestnik S, Philips N, et al. Exportin 1 Inhibition Induces Nerve Growth Factor Receptor Expression to Inhibit the NF-κB Pathway in Preclinical Models of Pediatric High-Grade Glioma. Mol Cancer Ther. 2020;19:540-551 pubmed publisher
Yu T, Gan S, Zhu Q, Dai D, Li N, Wang H, et al. Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24. Nat Commun. 2019;10:4353 pubmed publisher
Waters M, Gupta A, Mockenhaupt K, Brown L, Biswas D, Kordula T. RelB acts as a molecular switch driving chronic inflammation in glioblastoma multiforme. Oncogenesis. 2019;8:37 pubmed publisher
Gupta A, Waters M, Biswas D, Brown L, Surace M, Floros C, et al. RelB controls adaptive responses of astrocytes during sterile inflammation. Glia. 2019;67:1449-1461 pubmed publisher
Jeon H, Ham S, Kim J, Jin X, Lee S, Shin Y, et al. Ly6G+ inflammatory cells enable the conversion of cancer cells to cancer stem cells in an irradiated glioblastoma model. Cell Death Differ. 2019;: pubmed publisher
Pereira D, Gomes S, Borralho P, Rodrigues C. MEK5/ERK5 activation regulates colon cancer stem-like cell properties. Cell Death Discov. 2019;5:68 pubmed publisher
Li Y, Mooney E, Holden S, Xia X, Cohen D, Walsh S, et al. A20 Orchestrates Inflammatory Response in the Oral Mucosa through Restraining NF-κB Activity. J Immunol. 2019;202:2044-2056 pubmed publisher
Yoon M, Kang S, Lee S, Woo T, Oh A, Park S, et al. p53 induces senescence through Lamin A/C stabilization-mediated nuclear deformation. Cell Death Dis. 2019;10:107 pubmed publisher
Alameda J, Ramirez A, Garcia Fernandez R, Navarro M, Page A, Segovia J, et al. Premature aging and cancer development in transgenic mice lacking functional CYLD. Aging (Albany NY). 2019;11:127-159 pubmed publisher
Enguita M, Razquin N, Pamplona R, Quiroga J, Prieto J, Fortes P. The cirrhotic liver is depleted of docosahexaenoic acid (DHA), a key modulator of NF-κB and TGFβ pathways in hepatic stellate cells. Cell Death Dis. 2019;10:14 pubmed publisher
Aloor J, Azzam K, Guardiola J, Gowdy K, Madenspacher J, Gabor K, et al. Leucine-rich repeats and calponin homology containing 4 (Lrch4) regulates the innate immune response. J Biol Chem. 2019;294:1997-2008 pubmed publisher
Murahashi Y, Yano F, Kobayashi H, Makii Y, Iba K, Yamashita T, et al. Intra-articular administration of IκBα kinase inhibitor suppresses mouse knee osteoarthritis via downregulation of the NF-κB/HIF-2α axis. Sci Rep. 2018;8:16475 pubmed publisher
Leonardi M, Perna E, Tronnolone S, Colecchia D, Chiariello M. Activated kinase screening identifies the IKBKE oncogene as a positive regulator of autophagy. Autophagy. 2018;:1-15 pubmed publisher
Choi J, Yeo I, Kim K, Choi W, Jung J, Han S, et al. K284-6111 prevents the amyloid beta-induced neuroinflammation and impairment of recognition memory through inhibition of NF-κB-mediated CHI3L1 expression. J Neuroinflammation. 2018;15:224 pubmed publisher
Prinz E, Aviram S, Aronheim A. WDR62 mediates TNFα-dependent JNK activation via TRAF2-MLK3 axis. Mol Biol Cell. 2018;29:2470-2480 pubmed publisher
Wang P, Liu Z, Zhang X, Li J, Sun L, Ju Z, et al. CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells. Protein Cell. 2018;9:945-965 pubmed publisher
Brenke J, Popowicz G, Schorpp K, Rothenaigner I, Roesner M, Meininger I, et al. Targeting TRAF6 E3 ligase activity with a small-molecule inhibitor combats autoimmunity. J Biol Chem. 2018;293:13191-13203 pubmed publisher
Kimura A, Kitajima M, Nishida K, Serada S, Fujimoto M, Naka T, et al. NQO1 inhibits the TLR-dependent production of selective cytokines by promoting IκB-ζ degradation. J Exp Med. 2018;215:2197-2209 pubmed publisher
Bigas J, Sevilla L, Carceller E, Boix J, Perez P. Epidermal glucocorticoid and mineralocorticoid receptors act cooperatively to regulate epidermal development and counteract skin inflammation. Cell Death Dis. 2018;9:588 pubmed publisher
Zhu K, Liang W, Ma Z, Xu D, Cao S, Lu X, et al. Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression. Cell Death Dis. 2018;9:500 pubmed publisher
Baek J, Cheon Y, Kwak S, Jun H, Yoon K, Lee M, et al. Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling. Exp Mol Med. 2018;50:50 pubmed publisher
Zhang X, Wang Y, Yuan J, Li N, Pei S, Xu J, et al. Macrophage/microglial Ezh2 facilitates autoimmune inflammation through inhibition of Socs3. J Exp Med. 2018;215:1365-1382 pubmed publisher
Annibaldi A, Wicky John S, Vanden Berghe T, Swatek K, Ruan J, Liccardi G, et al. Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2. Mol Cell. 2018;69:566-580.e5 pubmed publisher
Zhang F, Brenner M, Yang W, Wang P. A cold-inducible RNA-binding protein (CIRP)-derived peptide attenuates inflammation and organ injury in septic mice. Sci Rep. 2018;8:3052 pubmed publisher
Jiang M, Xu C, Guo Y, Luo Q, Li L, Liu H, et al. ?-arrestin 2 attenuates lipopolysaccharide-induced liver injury via inhibition of TLR4/NF-?B signaling pathway-mediated inflammation in mice. World J Gastroenterol. 2018;24:216-225 pubmed publisher
Lao Y, Yang K, Wang Z, Sun X, Zou Q, Yu X, et al. DeSUMOylation of MKK7 kinase by the SUMO2/3 protease SENP3 potentiates lipopolysaccharide-induced inflammatory signaling in macrophages. J Biol Chem. 2018;293:3965-3980 pubmed publisher
Tsuchiya Y, Osaki K, Kanamoto M, Nakao Y, Takahashi E, Higuchi T, et al. Distinct B Subunits of PP2A Regulate the NF-?B Signaling Pathway through Dephosphorylation of IKK?, I?B?, and RelA. FEBS Lett. 2017;: pubmed publisher
Meshram S, Paul D, Manne R, Choppara S, Sankaran G, Agrawal Y, et al. FBXO32 activates NF-κB through IκBα degradation in inflammatory and genotoxic stress. Int J Biochem Cell Biol. 2017;92:134-140 pubmed publisher
Yoon S, Kovalenko A, Bogdanov K, Wallach D. MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation. Immunity. 2017;47:51-65.e7 pubmed publisher
Petersheim D, Massaad M, Lee S, Scarselli A, Cancrini C, Moriya K, et al. Mechanisms of genotype-phenotype correlation in autosomal dominant anhidrotic ectodermal dysplasia with immune deficiency. J Allergy Clin Immunol. 2018;141:1060-1073.e3 pubmed publisher
Van T, Polykratis A, Straub B, Kondylis V, Papadopoulou N, Pasparakis M. Kinase-independent functions of RIPK1 regulate hepatocyte survival and liver carcinogenesis. J Clin Invest. 2017;127:2662-2677 pubmed publisher
van Wijk S, Fricke F, Herhaus L, Gupta J, Hötte K, Pampaloni F, et al. Linear ubiquitination of cytosolic Salmonella Typhimurium activates NF-?B and restricts bacterial proliferation. Nat Microbiol. 2017;2:17066 pubmed publisher
Lundh M, Bugliani M, Dahlby T, Chou D, Wagner B, Ghiasi S, et al. The immunoproteasome is induced by cytokines and regulates apoptosis in human islets. J Endocrinol. 2017;233:369-379 pubmed publisher
Choo M, Sano Y, Kim C, Yasuda K, Li X, Lin X, et al. TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. J Exp Med. 2017;214:1297-1311 pubmed publisher
Ju H, Ying H, Tian T, Ling J, Fu J, Lu Y, et al. Mutant Kras- and p16-regulated NOX4 activation overcomes metabolic checkpoints in development of pancreatic ductal adenocarcinoma. Nat Commun. 2017;8:14437 pubmed publisher
Chen Y, Li C, Xie H, Fan Y, Yang Z, Ma J, et al. Infiltrating mast cells promote renal cell carcinoma angiogenesis by modulating PI3K→︀AKT→︀GSK3β→︀AM signaling. Oncogene. 2017;36:2879-2888 pubmed publisher
Della Mina E, Borghesi A, Zhou H, Bougarn S, Boughorbel S, Israel L, et al. Inherited human IRAK-1 deficiency selectively impairs TLR signaling in fibroblasts. Proc Natl Acad Sci U S A. 2017;114:E514-E523 pubmed publisher
Rivera Serrano E, Sherry B. NF-?B activation is cell type-specific in the heart. Virology. 2017;502:133-143 pubmed publisher
Lin J, Kato M, Nagata K, Okuwaki M. Efficient DNA binding of NF-κB requires the chaperone-like function of NPM1. Nucleic Acids Res. 2017;45:3707-3723 pubmed publisher
Fern ndez Majada V, Welz P, Ermolaeva M, Schell M, Adam A, Dietlein F, et al. The tumour suppressor CYLD regulates the p53 DNA damage response. Nat Commun. 2016;7:12508 pubmed publisher
Oh B, Kim S, Lee Y, Hong H, Kim T, Kim S, et al. Twist1-induced epithelial-mesenchymal transition according to microsatellite instability status in colon cancer cells. Oncotarget. 2016;7:57066-57076 pubmed publisher
Zhu L, Luo T, Xu X, Guo Y, Zhao X, Wang T, et al. E3 ubiquitin ligase Cbl-b negatively regulates C-type lectin receptor-mediated antifungal innate immunity. J Exp Med. 2016;213:1555-70 pubmed publisher
Jiang C, Zhou Z, Quan Y, Zhang S, Wang T, Zhao X, et al. CARMA3 Is a Host Factor Regulating the Balance of Inflammatory and Antiviral Responses against Viral Infection. Cell Rep. 2016;14:2389-401 pubmed publisher
Fauster A, Rebsamen M, Huber K, Bigenzahn J, Stukalov A, Lardeau C, et al. A cellular screen identifies ponatinib and pazopanib as inhibitors of necroptosis. Cell Death Dis. 2015;6:e1767 pubmed publisher
Kearns J, Basak S, Werner S, Huang C, Hoffmann A. IkappaBepsilon provides negative feedback to control NF-kappaB oscillations, signaling dynamics, and inflammatory gene expression. J Cell Biol. 2006;173:659-64 pubmed
Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt Supprian M, et al. Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury. J Clin Invest. 2005;115:849-59 pubmed
Xanthoulea S, Pasparakis M, Kousteni S, Brakebusch C, Wallach D, Bauer J, et al. Tumor necrosis factor (TNF) receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases. J Exp Med. 2004;200:367-76 pubmed
Matsushima A, Kaisho T, Rennert P, Nakano H, Kurosawa K, Uchida D, et al. Essential role of nuclear factor (NF)-kappaB-inducing kinase and inhibitor of kappaB (IkappaB) kinase alpha in NF-kappaB activation through lymphotoxin beta receptor, but not through tumor necrosis factor receptor I. J Exp Med. 2001;193:631-6 pubmed
Bajpai U, Zhang K, Teutsch M, Sen R, Wortis H. Bruton's tyrosine kinase links the B cell receptor to nuclear factor kappaB activation. J Exp Med. 2000;191:1735-44 pubmed
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA