product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
H-Ras (259) Antibody
catalog :
sc-35
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
259
reactivity :
human, mouse, pigs , rhesus macaque
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 76
Published Application/Species/Sample/DilutionReference
  • western blot; pigs ; fig 2
  • western blot; rhesus macaque; fig 2
Simões M, Martins C, Ferreira F. Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus. Virus Res. 2015;210:1-7 pubmed publisher
  • western blot; human
Funes J, Quintero M, Henderson S, Martinez D, Qureshi U, Westwood C, et al. Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production. Proc Natl Acad Sci U S A. 2007;104:6223-8 pubmed
Zhou M, Wiener H, Su W, Zhou Y, Liot C, Ahearn I, et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. J Cell Biol. 2016;214:445-58 pubmed publisher
Fritsch K, Mernberger M, Nist A, Stiewe T, Brehm A, Jacob R. Galectin-3 interacts with components of the nuclear ribonucleoprotein complex. BMC Cancer. 2016;16:502 pubmed publisher
Saha S, Choi H, Kim B, Dayem A, Yang G, Kim K, et al. KRT19 directly interacts with β-catenin/RAC1 complex to regulate NUMB-dependent NOTCH signaling pathway and breast cancer properties. Oncogene. 2017;36:332-349 pubmed publisher
Ohsaki Y, Kawai T, Yoshikawa Y, Cheng J, Jokitalo E, Fujimoto T. PML isoform II plays a critical role in nuclear lipid droplet formation. J Cell Biol. 2016;212:29-38 pubmed publisher
McCuaig R, Dunn J, Li J, Masch A, Knaute T, Schutkowski M, et al. PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation. Front Immunol. 2015;6:562 pubmed publisher
Walker E, Jensen L, Croft S, Ghildyal R. Variation in the nuclear effects of infection by different human rhinovirus serotypes. Front Microbiol. 2015;6:875 pubmed publisher
Rodríguez Muñoz R, Cárdenas Aguayo M, Alemán V, Osorio B, Chávez González O, Rendon A, et al. Novel Nuclear Protein Complexes of Dystrophin 71 Isoforms in Rat Cultured Hippocampal GABAergic and Glutamatergic Neurons. PLoS ONE. 2015;10:e0137328 pubmed publisher
Stockley J, Markert E, Zhou Y, Robson C, Elliott D, Lindberg J, et al. The RNA-binding protein Sam68 regulates expression and transcription function of the androgen receptor splice variant AR-V7. Sci Rep. 2015;5:13426 pubmed publisher
Schraivogel D, Schindler S, Danner J, Kremmer E, Pfaff J, Hannus S, et al. Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. Nucleic Acids Res. 2015;43:7447-61 pubmed publisher
Díaz Hernández J, Sebastián Serrano A, Gomez Villafuertes R, Diaz Hernandez M, Miras Portugal M. Age-related nuclear translocation of P2X6 subunit modifies splicing activity interacting with splicing factor 3A1. PLoS ONE. 2015;10:e0123121 pubmed publisher
Chang S, Chang W, Lu C, Tarn W. Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res. 2014;42:13788-98 pubmed publisher
Janardhan S, Marks R, Gajewski T. Primary murine CD4+ T cells fail to acquire the ability to produce effector cytokines when active Ras is present during Th1/Th2 differentiation. PLoS ONE. 2014;9:e112831 pubmed publisher
Guida M, Maraldi T, Beretti F, Follo M, Manzoli L, De Pol A. Nuclear Nox4-derived reactive oxygen species in myelodysplastic syndromes. Biomed Res Int. 2014;2014:456937 pubmed publisher
Choudhury R, Roy S, Tsai Y, Tripathy A, Graves L, Wang Z. The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 2014;5:3078 pubmed publisher
Ping X, Sun B, Wang L, Xiao W, Yang X, Wang W, et al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Res. 2014;24:177-89 pubmed publisher
Derlig K, Gießl A, Brandstätter J, Enz R, Dahlhaus R. Identification and characterisation of Simiate, a novel protein linked to the fragile X syndrome. PLoS ONE. 2013;8:e83007 pubmed publisher
Mbonye U, Gokulrangan G, Datt M, Dobrowolski C, Cooper M, Chance M, et al. Phosphorylation of CDK9 at Ser175 enhances HIV transcription and is a marker of activated P-TEFb in CD4(+) T lymphocytes. PLoS Pathog. 2013;9:e1003338 pubmed publisher
Vivarelli S, Lenzken S, Ruepp M, Ranzini F, Maffioletti A, Alvarez R, et al. Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. PLoS ONE. 2013;8:e61980 pubmed publisher
Werwein E, Dzuganova M, Usadel C, Klempnauer K. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis. 2013;4:e511 pubmed publisher
Li S, Guo W, Dewey C, Greaser M. Rbm20 regulates titin alternative splicing as a splicing repressor. Nucleic Acids Res. 2013;41:2659-72 pubmed publisher
Gu B, Eick D, Bensaude O. CTD serine-2 plays a critical role in splicing and termination factor recruitment to RNA polymerase II in vivo. Nucleic Acids Res. 2013;41:1591-603 pubmed publisher
Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
Layerenza J, Gonzalez P, García de Bravo M, Polo M, Sisti M, Ves Losada A. Nuclear lipid droplets: a novel nuclear domain. Biochim Biophys Acta. 2013;1831:327-40 pubmed publisher
Jeong W, Rho J, Yoon Y, Yoo S, Jeong N, Ryu W, et al. Cytoplasmic and nuclear anti-apoptotic roles of ?B-crystallin in retinal pigment epithelial cells. PLoS ONE. 2012;7:e45754 pubmed publisher
Kumar P, Kumar P, Lindberg L, Thirkill T, Ji J, Martsching L, et al. The MUC1 extracellular domain subunit is found in nuclear speckles and associates with spliceosomes. PLoS ONE. 2012;7:e42712 pubmed publisher
Kasper S, Breitenbuecher F, Heidel F, Hoffarth S, Markova B, Schuler M, et al. Targeting MCL-1 sensitizes FLT3-ITD-positive leukemias to cytotoxic therapies. Blood Cancer J. 2012;2:e60 pubmed publisher
Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, et al. BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signaling in angiogenesis. Blood. 2012;119:2688-98 pubmed publisher
Janardhan S, Praveen K, Marks R, Gajewski T. Evidence implicating the Ras pathway in multiple CD28 costimulatory functions in CD4+ T cells. PLoS ONE. 2011;6:e24931 pubmed publisher
Lenos K, de Lange J, Teunisse A, Lodder K, Verlaan de Vries M, Wiercinska E, et al. Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblasts. Mol Cancer. 2011;10:111 pubmed publisher
Puppin C, Passon N, Lavarone E, Di Loreto C, Frasca F, Vella V, et al. Levels of histone acetylation in thyroid tumors. Biochem Biophys Res Commun. 2011;411:679-83 pubmed publisher
Ballester M, Rodríguez Cariño C, Perez M, Gallardo C, Rodriguez J, Salas M, et al. Disruption of nuclear organization during the initial phase of African swine fever virus infection. J Virol. 2011;85:8263-9 pubmed publisher
Nagarkatti Gude D, Jaimez R, Henderson S, Teves M, Zhang Z, Strauss J. Spag16, an axonemal central apparatus gene, encodes a male germ cell nuclear speckle protein that regulates SPAG16 mRNA expression. PLoS ONE. 2011;6:e20625 pubmed publisher
Qian W, Liang H, Shi J, Jin N, Grundke Iqbal I, Iqbal K, et al. Regulation of the alternative splicing of tau exon 10 by SC35 and Dyrk1A. Nucleic Acids Res. 2011;39:6161-71 pubmed publisher
Dai Y, Walker S, de Vet E, Cook S, Welch H, Lockyer P. Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities. J Biol Chem. 2011;286:19905-16 pubmed publisher
Harrigan J, Belotserkovskaya R, Coates J, Dimitrova D, Polo S, Bradshaw C, et al. Replication stress induces 53BP1-containing OPT domains in G1 cells. J Cell Biol. 2011;193:97-108 pubmed publisher
Scharf A, Grozdanov P, Veith R, Kubitscheck U, Meier U, von Mikecz A. Distant positioning of proteasomal proteolysis relative to actively transcribed genes. Nucleic Acids Res. 2011;39:4612-27 pubmed publisher
Kim Y, Lee J, Oh K, Araki M, Araki K, Yamamura K, et al. NSrp70 is a novel nuclear speckle-related protein that modulates alternative pre-mRNA splicing in vivo. Nucleic Acids Res. 2011;39:4300-14 pubmed publisher
Sytnikova Y, Kubarenko A, Sch fer A, Weber A, Niehrs C. Gadd45a is an RNA binding protein and is localized in nuclear speckles. PLoS ONE. 2011;6:e14500 pubmed publisher
Shevtsov S, Dundr M. Nucleation of nuclear bodies by RNA. Nat Cell Biol. 2011;13:167-73 pubmed publisher
Kaddoum L, Magdeleine E, Waldo G, Joly E, Cabantous S. One-step split GFP staining for sensitive protein detection and localization in mammalian cells. Biotechniques. 2010;49:727-8, 730, 732 passim pubmed publisher
Belzile J, Abrahamyan L, Gerard F, Rougeau N, Cohen E. Formation of mobile chromatin-associated nuclear foci containing HIV-1 Vpr and VPRBP is critical for the induction of G2 cell cycle arrest. PLoS Pathog. 2010;6:e1001080 pubmed publisher
Favale N, Fernández Tome M, Pescio L, Sterin Speziale N. The rate-limiting enzyme in phosphatidylcholine synthesis is associated with nuclear speckles under stress conditions. Biochim Biophys Acta. 2010;1801:1184-94 pubmed publisher
Wang X, He S, Sun J, Delcuve G, Davie J. Selective association of peroxiredoxin 1 with genomic DNA and COX-2 upstream promoter elements in estrogen receptor negative breast cancer cells. Mol Biol Cell. 2010;21:2987-95 pubmed publisher
Huen M, Sy S, Leung K, Ching Y, Tipoe G, Man C, et al. SON is a spliceosome-associated factor required for mitotic progression. Cell Cycle. 2010;9:2679-85 pubmed publisher
Ohshiro K, Mudvari P, Meng Q, Rayala S, Sahin A, Fuqua S, et al. Identification of a novel estrogen receptor-alpha variant and its upstream splicing regulator. Mol Endocrinol. 2010;24:914-22 pubmed publisher
Navaraj A, Finnberg N, Dicker D, Yang W, Matthew E, El Deiry W. Reduced cell death, invasive and angiogenic features conferred by BRCA1-deficiency in mammary epithelial cells transformed with H-Ras. Cancer Biol Ther. 2009;8:2417-44 pubmed
Rajan P, Dalgliesh C, Bourgeois C, Heiner M, Emami K, Clark E, et al. Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 Nuclear Bodies. BMC Cell Biol. 2009;10:82 pubmed publisher
Moumtzi S, Roberts M, Joyce T, Evangelidou M, Probert L, Frillingos S, et al. Gene expression profile associated with oncogenic ras-induced senescence, cell death, and transforming properties in human cells. Cancer Invest. 2010;28:563-87 pubmed publisher
Hancock R, Hadj Sahraoui Y. Isolation of cell nuclei using inert macromolecules to mimic the crowded cytoplasm. PLoS ONE. 2009;4:e7560 pubmed publisher
Zolotukhin A, Uranishi H, Lindtner S, Bear J, Pavlakis G, Felber B. Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA. Nucleic Acids Res. 2009;37:7151-62 pubmed publisher
Wang X, Belguise K, O Neill C, Sánchez Morgan N, Romagnoli M, Eddy S, et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol. 2009;29:3832-44 pubmed publisher
Garcia M, Byfield R, Robek M. Hepatitis B virus replication and release are independent of core lysine ubiquitination. J Virol. 2009;83:4923-33 pubmed publisher
Tousseyn T, Thathiah A, Jorissen E, Raemaekers T, Konietzko U, Reiss K, et al. ADAM10, the rate-limiting protease of regulated intramembrane proteolysis of Notch and other proteins, is processed by ADAMS-9, ADAMS-15, and the gamma-secretase. J Biol Chem. 2009;284:11738-47 pubmed publisher
Yan P, Fu J, Qu Z, Li S, Tanaka T, Grusby M, et al. PDLIM2 suppresses human T-cell leukemia virus type I Tax-mediated tumorigenesis by targeting Tax into the nuclear matrix for proteasomal degradation. Blood. 2009;113:4370-80 pubmed publisher
Tapia R, Huerta M, Islas S, Avila Flores A, Lopez Bayghen E, Weiske J, et al. Zona occludens-2 inhibits cyclin D1 expression and cell proliferation and exhibits changes in localization along the cell cycle. Mol Biol Cell. 2009;20:1102-17 pubmed publisher
MacPherson M, Beatty L, Zhou W, Du M, Sadowski P. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol. 2009;29:714-25 pubmed publisher
Salsman J, Zimmerman N, Chen T, Domagala M, Frappier L. Genome-wide screen of three herpesviruses for protein subcellular localization and alteration of PML nuclear bodies. PLoS Pathog. 2008;4:e1000100 pubmed publisher
Xu M, Cook P. Similar active genes cluster in specialized transcription factories. J Cell Biol. 2008;181:615-23 pubmed publisher
Konietzko U, Goodger Z, Meyer M, Kohli B, Bosset J, Lahiri D, et al. Co-localization of the amyloid precursor protein and Notch intracellular domains in nuclear transcription factories. Neurobiol Aging. 2010;31:58-73 pubmed publisher
Huerta M, Munoz R, Tapia R, Soto Reyes E, Ramírez L, Recillas Targa F, et al. Cyclin D1 is transcriptionally down-regulated by ZO-2 via an E box and the transcription factor c-Myc. Mol Biol Cell. 2007;18:4826-36 pubmed
Tanaka T, Grusby M, Kaisho T. PDLIM2-mediated termination of transcription factor NF-kappaB activation by intranuclear sequestration and degradation of the p65 subunit. Nat Immunol. 2007;8:584-91 pubmed
Kobayakawa S, Miike K, Nakao M, Abe K. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells. Genes Cells. 2007;12:447-60 pubmed
Hivin P, Basbous J, Raymond F, Henaff D, Arpin André C, Robert Hebmann V, et al. The HBZ-SP1 isoform of human T-cell leukemia virus type I represses JunB activity by sequestration into nuclear bodies. Retrovirology. 2007;4:14 pubmed
Brooks W, Banerjee S, Crawford D. G2E3 is a nucleo-cytoplasmic shuttling protein with DNA damage responsive localization. Exp Cell Res. 2007;313:665-76 pubmed
Psahoulia F, Moumtzi S, Roberts M, Sasazuki T, Shirasawa S, Pintzas A. Quercetin mediates preferential degradation of oncogenic Ras and causes autophagy in Ha-RAS-transformed human colon cells. Carcinogenesis. 2007;28:1021-31 pubmed
Markus M, Morris B. Lark is the splicing factor RBM4 and exhibits unique subnuclear localization properties. DNA Cell Biol. 2006;25:457-64 pubmed
Watzke A, Köhn M, Gutierrez Rodriguez M, Wacker R, Schröder H, Breinbauer R, et al. Site-selective protein immobilization by Staudinger ligation. Angew Chem Int Ed Engl. 2006;45:1408-12 pubmed
Barboric M, Kohoutek J, Price J, Blazek D, Price D, Peterlin B. Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb. EMBO J. 2005;24:4291-303 pubmed
Boito R, Menniti M, Amato R, Palmieri C, Marinaro C, Iuliano R, et al. RFX-1, a putative alpha Adducin interacting protein in a human kidney library. FEBS Lett. 2005;579:6439-43 pubmed
Roberts M, Drosopoulos K, Vasileiou I, Stricker M, Taoufik E, Maercker C, et al. Microarray analysis of the differential transformation mediated by Kirsten and Harvey Ras oncogenes in a human colorectal adenocarcinoma cell line. Int J Cancer. 2006;118:616-27 pubmed
Abu Baker A, Laganiere S, Fan X, Laganiere J, Brais B, Rouleau G. Cytoplasmic targeting of mutant poly(A)-binding protein nuclear 1 suppresses protein aggregation and toxicity in oculopharyngeal muscular dystrophy. Traffic. 2005;6:766-79 pubmed
Miele A, Braastad C, Holmes W, Mitra P, Medina R, Xie R, et al. HiNF-P directly links the cyclin E/CDK2/p220NPAT pathway to histone H4 gene regulation at the G1/S phase cell cycle transition. Mol Cell Biol. 2005;25:6140-53 pubmed
Vemuganti R, Dempsey R. Carotid atherosclerotic plaques from symptomatic stroke patients share the molecular fingerprints to develop in a neoplastic fashion: a microarray analysis study. Neuroscience. 2005;131:359-74 pubmed
Kamachi M, Le T, Kim S, Geiger M, Anderson P, Utz P. Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway. J Exp Med. 2002;196:1213-25 pubmed
product information
Catalog Number :
sc-35
Product Name :
H-Ras (259) Antibody
Product Type :
Monoclonal
Host Species :
rat
Clonality :
monoclonal
Clone Name :
259
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
H-Ras
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, FCM, ELISA
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA