product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
K-Ras (F234) Antibody
catalog :
sc-30
quantity :
100 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F234
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation, western blot knockout validation
citations: 119
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:200; fig 1a
Ischenko I, D Amico S, Rao M, Li J, Hayman M, Powers S, et al. KRAS drives immune evasion in a genetic model of pancreatic cancer. Nat Commun. 2021;12:1482 pubmed publisher
  • western blot knockout validation; mouse; fig 1
Fotiadou P, Takahashi C, Rajabi H, Ewen M. Wild-type NRas and KRas perform distinct functions during transformation. Mol Cell Biol. 2007;27:6742-55 pubmed
  • western blot; mouse; fig 2d
Kasuga A, Semba T, Sato R, Nobusue H, Sugihara E, Takaishi H, et al. Oncogenic KRAS-expressing organoids with biliary epithelial stem cell properties give rise to biliary tract cancer in mice. Cancer Sci. 2021;112:1822-1838 pubmed publisher
  • western blot; human; 1:500; loading ...; fig s1i
Kennedy S, Jarboui M, Srihari S, Raso C, Bryan K, Dernayka L, et al. Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRASG13D. Nat Commun. 2020;11:499 pubmed publisher
  • western blot; human; fig 9g
Kabayama H, Takeuchi M, Tokushige N, Muramatsu S, Kabayama M, Fukuda M, et al. An ultra-stable cytoplasmic antibody engineered for in vivo applications. Nat Commun. 2020;11:336 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 4f
Ramírez C, Hauser A, Vucic E, Bar Sagi D. Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis. Nature. 2019;576:477-481 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s1d
Bueno M, Jimenez Renard V, Samino S, Capellades J, Junza A, López Rodríguez M, et al. Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells. Nat Commun. 2019;10:5011 pubmed publisher
  • western blot; mouse; loading ...; fig 3d
Yao W, Rose J, Wang W, Seth S, Jiang H, Taguchi A, et al. Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer. Nature. 2019;: pubmed publisher
  • western blot; human; loading ...; fig 3e
Lee S, Cho Y, Cha P, Yoon J, Ro E, Jeong W, et al. A small molecule approach to degrade RAS with EGFR repression is a potential therapy for KRAS mutation-driven colorectal cancer resistance to cetuximab. Exp Mol Med. 2018;50:153 pubmed publisher
  • western blot; mouse; fig 1a
Ambrogio C, Köhler J, Zhou Z, Wang H, Paranal R, Li J, et al. KRAS Dimerization Impacts MEK Inhibitor Sensitivity and Oncogenic Activity of Mutant KRAS. Cell. 2018;172:857-868.e15 pubmed publisher
  • immunoprecipitation; human; loading ...; tbl 1
  • immunocytochemistry; human; loading ...; tbl 1
  • western blot; human; fig 1d
Waters A, Ozkan Dagliyan I, Vaseva A, Fer N, Strathern L, Hobbs G, et al. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. Sci Signal. 2017;10: pubmed publisher
  • western blot; human; loading ...; fig 2c
Chen X, Wu Q, Depeille P, Chen P, Thornton S, Kalirai H, et al. RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma. Cancer Cell. 2017;31:685-696.e6 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 3c
Shen H, Xing C, Cui K, Li Y, Zhang J, Du R, et al. MicroRNA-30a attenuates mutant KRAS-driven colorectal tumorigenesis via direct suppression of ME1. Cell Death Differ. 2017;24:1253-1262 pubmed publisher
  • western blot; human; fig 5a
Taoka R, Jinesh G, Xue W, Safe S, Kamat A. CF3DODA-Me induces apoptosis, degrades Sp1, and blocks the transformation phase of the blebbishield emergency program. Apoptosis. 2017;22:719-729 pubmed publisher
  • western blot; human; loading ...; fig 3b; 4a
  • western blot; mouse; loading ...; fig 3e
Forzati F, De Martino M, Esposito F, Sepe R, Pellecchia S, Malapelle U, et al. miR-155 is positively regulated by CBX7 in mouse embryonic fibroblasts and colon carcinomas, and targets the KRAS oncogene. BMC Cancer. 2017;17:170 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 2d
Vallejo A, Perurena N, Guruceaga E, Mazur P, Martínez Canarias S, Zandueta C, et al. An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer. Nat Commun. 2017;8:14294 pubmed publisher
  • western blot; human; loading ...; fig 7a
Dey K, Bharti R, Dey G, Pal I, Rajesh Y, Chavan S, et al. S100A7 has an oncogenic role in oral squamous cell carcinoma by activating p38/MAPK and RAB2A signaling pathway. Cancer Gene Ther. 2016;23:382-391 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 6h
Jinesh G, Molina J, Huang L, Laing N, Mills G, Bar Eli M, et al. Mitochondrial oligomers boost glycolysis in cancer stem cells to facilitate blebbishield-mediated transformation after apoptosis. Cell Death Discov. 2016;2:16003 pubmed publisher
  • western blot; human; loading ...; fig 1b
Li Y, Dillon T, Takahashi M, Earley K, Stork P. Protein Kinase A-independent Ras Protein Activation Cooperates with Rap1 Protein to Mediate Activation of the Extracellular Signal-regulated Kinases (ERK) by cAMP. J Biol Chem. 2016;291:21584-21595 pubmed
  • western blot; mouse; 1:1000
Weaver R, Limzerwala J, Naylor R, Jeganathan K, Baker D, van Deursen J. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. elife. 2016;5: pubmed publisher
  • western blot; human; 1:1000; fig s2
Anta B, Pérez Rodríguez A, Castro J, García Domínguez C, Ibiza S, Martínez N, et al. PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes. Cell Death Dis. 2016;7:e2311 pubmed publisher
  • western blot; mouse; fig 7
Petrova L, Gran C, Bjoras M, Doetsch P. Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells. PLoS ONE. 2016;11:e0158581 pubmed publisher
  • western blot; human; fig 2 a',' b
Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed publisher
  • western blot; human; 1:1000; fig 2
Tsang Y, Dogruluk T, Tedeschi P, Wardwell Ozgo J, Lu H, Espitia M, et al. Functional annotation of rare gene aberration drivers of pancreatic cancer. Nat Commun. 2016;7:10500 pubmed publisher
  • western blot; human; fig 1
Chung S, Moon H, Ju H, Kim D, Cho K, Ribback S, et al. Comparison of liver oncogenic potential among human RAS isoforms. Oncotarget. 2016;7:7354-66 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Chen Y, Zheng Y, You X, Yu M, Fu G, Su X, et al. Kras Is Critical for B Cell Lymphopoiesis. J Immunol. 2016;196:1678-85 pubmed publisher
  • western blot; human; fig 1
Kim J, Sato M, Choi J, Kim H, Yeh B, Larsen J, et al. Nuclear Receptor Expression and Function in Human Lung Cancer Pathogenesis. PLoS ONE. 2015;10:e0134842 pubmed publisher
  • western blot; human; fig 6
Brito H, Martins A, Lavrado J, Mendes E, Francisco A, Santos S, et al. Targeting KRAS Oncogene in Colon Cancer Cells with 7-Carboxylate Indolo[3,2-b]quinoline Tri-Alkylamine Derivatives. PLoS ONE. 2015;10:e0126891 pubmed publisher
  • western blot; human; 1:200; fig 1
Huang L, Counter C. Reduced HRAS G12V-Driven Tumorigenesis of Cell Lines Expressing KRAS C118S. PLoS ONE. 2015;10:e0123918 pubmed publisher
  • western blot; mouse; 1:1000
Hu S, Danilov A, Godek K, Orr B, Tafe L, Rodriguez Canales J, et al. CDK2 Inhibition Causes Anaphase Catastrophe in Lung Cancer through the Centrosomal Protein CP110. Cancer Res. 2015;75:2029-38 pubmed publisher
  • western blot; human; 1:25; fig 2c
Stolze B, Reinhart S, Bulllinger L, Fröhling S, Scholl C. Comparative analysis of KRAS codon 12, 13, 18, 61, and 117 mutations using human MCF10A isogenic cell lines. Sci Rep. 2015;5:8535 pubmed publisher
  • western blot; rat; 1:200; loading ...; fig 3
Gao J, Du J, Wang Y, Li J, Wei L, Guo M. Synergistic effects of curcumin and bevacizumab on cell signaling pathways in hepatocellular carcinoma. Oncol Lett. 2015;9:295-299 pubmed
  • western blot; mouse; 1:200; fig 1
Huang L, Carney J, Cardona D, Counter C. Decreased tumorigenesis in mice with a Kras point mutation at C118. Nat Commun. 2014;5:5410 pubmed publisher
  • western blot; human
Fujimura K, Wright T, Strnadel J, Kaushal S, Metildi C, Lowy A, et al. A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer. Cancer Res. 2014;74:6671-81 pubmed publisher
  • western blot; mouse; fig 4
Song J, An N, Chatterjee S, Kistner Griffin E, Mahajan S, Mehrotra S, et al. Deletion of Pim kinases elevates the cellular levels of reactive oxygen species and sensitizes to K-Ras-induced cell killing. Oncogene. 2015;34:3728-36 pubmed publisher
  • western blot; human; fig 5
Patel A, Burton D, Halvorsen K, Balkan W, Reiner T, Perez Stable C, et al. MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene. 2015;34:2586-96 pubmed publisher
  • western blot; human; 1:100; fig 6
Bauckman K, Haller E, Flores I, Nanjundan M. Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells. Cell Death Dis. 2013;4:e592 pubmed publisher
  • western blot; human
BENTLEY C, Jurinka S, Kljavin N, Vartanian S, Ramani S, Gonzalez L, et al. A requirement for wild-type Ras isoforms in mutant KRas-driven signalling and transformation. Biochem J. 2013;452:313-20 pubmed publisher
  • western blot; human
Vartanian S, BENTLEY C, Brauer M, Li L, Shirasawa S, Sasazuki T, et al. Identification of mutant K-Ras-dependent phenotypes using a panel of isogenic cell lines. J Biol Chem. 2013;288:2403-13 pubmed publisher
Neu J, Dziunycz P, Dzung A, Lefort K, Falke M, Denzler R, et al. miR-181a decelerates proliferation in cutaneous squamous cell carcinoma by targeting the proto-oncogene KRAS. PLoS ONE. 2017;12:e0185028 pubmed publisher
Takahashi M, Li Y, Dillon T, Kariya Y, Stork P. Phosphorylation of the C-Raf N Region Promotes Raf Dimerization. Mol Cell Biol. 2017;37: pubmed publisher
Sun T, Liu S, Zhou Y, Yao Z, Zhang D, Cao S, et al. Evolutionary biologic changes of gut microbiota in an 'adenoma-carcinoma sequence' mouse colorectal cancer model induced by 1, 2-Dimethylhydrazine. Oncotarget. 2017;8:444-457 pubmed publisher
Shimizu K, Kobayashi Y, Nakatsuji E, Yamazaki M, Shimba S, Sakimura K, et al. SCOP/PHLPP1? mediates circadian regulation of long-term recognition memory. Nat Commun. 2016;7:12926 pubmed publisher
Caiola E, Brunelli L, Marabese M, Broggini M, Lupi M, Pastorelli R. Different metabolic responses to PI3K inhibition in NSCLC cells harboring wild-type and G12C mutant KRAS. Oncotarget. 2016;7:51462-51472 pubmed publisher
Klein K, Werner K, Teske C, Schenk M, Giese T, Weitz J, et al. Role of TFEB-driven autophagy regulation in pancreatic cancer treatment. Int J Oncol. 2016;49:164-72 pubmed publisher
Song C, Liu L, Pei X, Liu X, Yang L, Ye F, et al. miR-200c inhibits breast cancer proliferation by targeting KRAS. Oncotarget. 2015;6:34968-78 pubmed publisher
Unni A, Lockwood W, Zejnullahu K, Lee Lin S, Varmus H. Evidence that synthetic lethality underlies the mutual exclusivity of oncogenic KRAS and EGFR mutations in lung adenocarcinoma. elife. 2015;4:e06907 pubmed publisher
Jin X, Sun Y, Yang H, Li J, Yu S, Chang X, et al. Deregulation of the MiR-193b-KRAS Axis Contributes to Impaired Cell Growth in Pancreatic Cancer. PLoS ONE. 2015;10:e0125515 pubmed publisher
Ju H, Calvisi D, Moon H, Baek S, Ribback S, Dombrowski F, et al. Transgenic mouse model expressing P53(R172H), luciferase, EGFP, and KRAS(G12D) in a single open reading frame for live imaging of tumor. Sci Rep. 2015;5:8053 pubmed publisher
Sun Y, Chen C, Zhang P, Xie H, Hou L, Hui Z, et al. Reduced miR-3127-5p expression promotes NSCLC proliferation/invasion and contributes to dasatinib sensitivity via the c-Abl/Ras/ERK pathway. Sci Rep. 2014;4:6527 pubmed publisher
Qi X, Xie C, Hou S, Li G, Yin N, Dong L, et al. Identification of a ternary protein-complex as a therapeutic target for K-Ras-dependent colon cancer. Oncotarget. 2014;5:4269-82 pubmed
Okada M, Shibuya K, Sato A, Seino S, Suzuki S, Seino M, et al. Targeting the K-Ras--JNK axis eliminates cancer stem-like cells and prevents pancreatic tumor formation. Oncotarget. 2014;5:5100-12 pubmed
Shukla S, Allam U, Ahsan A, Chen G, Krishnamurthy P, Marsh K, et al. KRAS protein stability is regulated through SMURF2: UBCH5 complex-mediated β-TrCP1 degradation. Neoplasia. 2014;16:115-28 pubmed publisher
Ceccarelli V, Nocentini G, Billi M, Racanicchi S, Riccardi C, Roberti R, et al. Eicosapentaenoic acid activates RAS/ERK/C/EBP? pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. PLoS ONE. 2014;9:e85025 pubmed publisher
Le Floc h A, Tanaka Y, Bantilan N, Voisinne G, Altan Bonnet G, Fukui Y, et al. Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapse. J Exp Med. 2013;210:2721-37 pubmed publisher
Azrak S, Ginel Picardo A, Drosten M, Barbacid M, Santos E. Reversible, interrelated mRNA and miRNA expression patterns in the transcriptome of Rasless fibroblasts: functional and mechanistic implications. BMC Genomics. 2013;14:731 pubmed publisher
Johnson J, de Mejia E. Flavonoid apigenin modified gene expression associated with inflammation and cancer and induced apoptosis in human pancreatic cancer cells through inhibition of GSK-3?/NF-?B signaling cascade. Mol Nutr Food Res. 2013;57:2112-27 pubmed publisher
Commisso C, Davidson S, Soydaner Azeloglu R, Parker S, Kamphorst J, Hackett S, et al. Macropinocytosis of protein is an amino acid supply route in Ras-transformed cells. Nature. 2013;497:633-7 pubmed publisher
Son J, Lyssiotis C, Ying H, Wang X, Hua S, Ligorio M, et al. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Nature. 2013;496:101-5 pubmed publisher
Valtorta E, Misale S, Sartore Bianchi A, Nagtegaal I, Paraf F, Lauricella C, et al. KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy. Int J Cancer. 2013;133:1259-65 pubmed publisher
Huang H, Daniluk J, Liu Y, Chu J, Li Z, Ji B, et al. Oncogenic K-Ras requires activation for enhanced activity. Oncogene. 2014;33:532-5 pubmed publisher
Ma Y, Gu Y, Zhang Q, Han Y, Yu S, Lu Z, et al. Targeted degradation of KRAS by an engineered ubiquitin ligase suppresses pancreatic cancer cell growth in vitro and in vivo. Mol Cancer Ther. 2013;12:286-94 pubmed publisher
Shao D, Tsherniak A, Gopal S, Weir B, Tamayo P, Stransky N, et al. ATARiS: computational quantification of gene suppression phenotypes from multisample RNAi screens. Genome Res. 2013;23:665-78 pubmed publisher
Wang Y, Ren T, Cai Y, He X. MicroRNA let-7a inhibits the proliferation and invasion of nonsmall cell lung cancer cell line 95D by regulating K-Ras and HMGA2 gene expression. Cancer Biother Radiopharm. 2013;28:131-7 pubmed publisher
Hofmann I, Weiss A, Elain G, Schwaederle M, Sterker D, Romanet V, et al. K-RAS mutant pancreatic tumors show higher sensitivity to MEK than to PI3K inhibition in vivo. PLoS ONE. 2012;7:e44146 pubmed publisher
Li C, Ge M, Yin Y, Luo M, Chen D. Silencing expression of ribosomal protein L26 and L29 by RNA interfering inhibits proliferation of human pancreatic cancer PANC-1 cells. Mol Cell Biochem. 2012;370:127-39 pubmed publisher
Ikonomou G, Kostourou V, Shirasawa S, Sasazuki T, Samiotaki M, Panayotou G. Interplay between oncogenic K-Ras and wild-type H-Ras in Caco2 cell transformation. J Proteomics. 2012;75:5356-69 pubmed publisher
Kent O, Fox Talbot K, Halushka M. RREB1 repressed miR-143/145 modulates KRAS signaling through downregulation of multiple targets. Oncogene. 2013;32:2576-85 pubmed publisher
Misale S, Yaeger R, Hobor S, Scala E, Janakiraman M, Liska D, et al. Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer. Nature. 2012;486:532-6 pubmed publisher
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Kim C, Vo M, Kim H, Lee H, Yoon N, Lee B, et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res. 2012;40:3856-69 pubmed publisher
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Psahoulia F, Moumtzi S, Roberts M, Sasazuki T, Shirasawa S, Pintzas A. Quercetin mediates preferential degradation of oncogenic Ras and causes autophagy in Ha-RAS-transformed human colon cells. Carcinogenesis. 2007;28:1021-31 pubmed
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product information
Catalog Number :
sc-30
Product Name :
K-Ras (F234) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
F234
Isotype :
IgG2a
Size :
100 µg/ml
List Price :
316 USD
Immunogen :
K-Ras
Specificity :
mouse, rat, human
Uses :
WB, IP, IF
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA