product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
PLZF (D-9) Antibody
catalog :
sc-28319
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D-9
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 45
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 4g
Qin J, Huang T, Wang J, Xu L, Dang Q, Xu X, et al. RAD51 is essential for spermatogenesis and male fertility in mice. Cell Death Discov. 2022;8:118 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2e
Huang G, Liu L, Wang H, Gou M, Gong P, Tian C, et al. Tet1 Deficiency Leads to Premature Reproductive Aging by Reducing Spermatogonia Stem Cells and Germ Cell Differentiation. iScience. 2020;23:100908 pubmed publisher
  • immunocytochemistry; mouse; 1:100; loading ...; fig 3b
Xie Y, Chen H, Luo D, Yang X, Yao J, Zhang C, et al. Inhibiting Necroptosis of Spermatogonial Stem Cell as a Novel Strategy for Male Fertility Preservation. Stem Cells Dev. 2020;29:475-487 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4g
Varuzhanyan G, Rojansky R, Sweredoski M, Graham R, Hess S, Ladinsky M, et al. Mitochondrial fusion is required for spermatogonial differentiation and meiosis. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3b
  • western blot; mouse; 1:1000; loading ...; fig 3d
Gao X, Chen H, Liu J, Shen S, Wang Q, Clement T, et al. The REGγ-Proteasome Regulates Spermatogenesis Partially by P53-PLZF Signaling. Stem Cell Reports. 2019;13:559-571 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1g
Cao J, Lin Z, Tong M, Zhang Y, Li Y, Zhou Y. Mechanistic target of rapamycin kinase (Mtor) is required for spermatogonial proliferation and differentiation in mice. Asian J Androl. 2020;22:169-176 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 7b
Katsumata O, Mori M, Sawane Y, Niimura T, Ito A, Okamoto H, et al. Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues. Histochem Cell Biol. 2017;148:577-596 pubmed publisher
  • flow cytometry; mouse; fig 5g
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 4c,4e
Liu W, Wang F, Xu Q, Shi J, Zhang X, Lu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 1
  • immunocytochemistry; mouse; 1:200; fig 1
Bai Y, Feng M, Liu S, Wei H, Li L, Zhang X, et al. Differential gene expression in mouse spermatogonial stem cells and embryonic stem cells. Int J Mol Med. 2016;38:423-32 pubmed publisher
  • immunocytochemistry; mouse; 1:100; fig '9a'
Suzuki A, Hirasaki M, Hishida T, Wu J, Okamura D, Ueda A, et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun. 2016;7:11056 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 3
Liu Y, Liu C, Chang Z, Wadas B, Brower C, Song Z, et al. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016;291:7426-38 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 4
Hu X, Tang Z, Li Y, Liu W, Zhang S, Wang B, et al. Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice. Sci Rep. 2015;5:12982 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 5
  • western blot; mouse; 1:500; fig 1
Xu J, Wan P, Wang M, Zhang J, Gao X, Hu B, et al. AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment. Cell Death Dis. 2015;6:e1818 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 1
  • western blot; mouse; fig 5
Ferder I, Wang N. Hypermaintenance and hypofunction of aged spermatogonia: insight from age-related increase of Plzf expression. Oncotarget. 2015;6:15891-901 pubmed
  • flow cytometry; mouse; fig 8
Pei B, Zhao M, Miller B, Véla J, Bruinsma M, Virgin H, et al. Invariant NKT cells require autophagy to coordinate proliferation and survival signals during differentiation. J Immunol. 2015;194:5872-84 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:500; fig 1
Xiao G, Unger P, Yang Q, Kinoshita Y, Singh K, McMahon L, et al. Loss of PLZF expression in prostate cancer by immunohistochemistry correlates with tumor aggressiveness and metastasis. PLoS ONE. 2015;10:e0121318 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • flow cytometry; mouse; 2 ug/ml
Dobenecker M, Kim J, Marcello J, Fang T, Prinjha R, Bosselut R, et al. Coupling of T cell receptor specificity to natural killer T cell development by bivalent histone H3 methylation. J Exp Med. 2015;212:297-306 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Jiang X, Ma T, Zhang Y, Zhang H, Yin S, Zheng W, et al. Specific deletion of Cdh2 in Sertoli cells leads to altered meiotic progression and subfertility of mice. Biol Reprod. 2015;92:79 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3
  • western blot; mouse; 1:200; fig 4
Gao X, Ma W, Nie J, Zhang C, Zhang J, Yao G, et al. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation. Cell Death Dis. 2015;6:e1610 pubmed publisher
  • immunohistochemistry - paraffin section; human
Sachs C, Robinson B, Andres Martin L, Webster T, Gilbert M, Lo H, et al. Evaluation of candidate spermatogonial markers ID4 and GPR125 in testes of adult human cadaveric organ donors. Andrology. 2014;2:607-14 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:1000
Momeni M, Kalir T, Farag S, Kinoshita Y, Roman T, Chuang L, et al. Immunohistochemical detection of promyelocytic leukemia zinc finger and histone 1.5 in uterine leiomyosarcoma and leiomyoma. Reprod Sci. 2014;21:1171-6 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Zhang J, Hatakeyama J, Eto K, Abe S. Reconstruction of a seminiferous tubule-like structure in a 3 dimensional culture system of re-aggregated mouse neonatal testicular cells within a collagen matrix. Gen Comp Endocrinol. 2014;205:121-32 pubmed publisher
  • immunohistochemistry; human; 1:1000
Hechtman J, Beasley M, Kinoshita Y, Ko H, Hao K, Burstein D. Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors: a pilot immunohistochemical study. Hum Pathol. 2013;44:1400-5 pubmed publisher
  • western blot; mouse; 1:50
Gallagher S, Kofman A, Huszar J, Dannenberg J, Depinho R, Braun R, et al. Distinct requirements for Sin3a in perinatal male gonocytes and differentiating spermatogonia. Dev Biol. 2013;373:83-94 pubmed publisher
Lee S, Park J, Lee D, Otsu K, Kim P, Mizuno S, et al. Mast4 knockout shows the regulation of spermatogonial stem cell self-renewal via the FGF2/ERM pathway. Cell Death Differ. 2021;28:1441-1454 pubmed publisher
Wong J, Chiang Y, Shih Y, Chiu C, Chen H, Shieh T, et al. Salvia sclarea L. Essential Oil Extract and Its Antioxidative Phytochemical Sclareol Inhibit Oxytocin-Induced Uterine Hypercontraction Dysmenorrhea Model by Inhibiting the Ca2+-MLCK-MLC20 Signaling Cascade: An Ex Vivo and In Vivo Study. Antioxidants (Basel). 2020;9: pubmed publisher
Huang L, Zhang Y, Li Z, Zhao X, Xi Z, Chen H, et al. MiR-4319 suppresses colorectal cancer progression by targeting ABTB1. United European Gastroenterol J. 2019;7:517-528 pubmed publisher
Maezawa S, Hasegawa K, Alavattam K, Funakoshi M, Sato T, Barski A, et al. SCML2 promotes heterochromatin organization in late spermatogenesis. J Cell Sci. 2018;131: pubmed publisher
Bellelli R, Borel V, Logan C, Svendsen J, Cox D, Nye E, et al. Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell. 2018;70:707-721.e7 pubmed publisher
Vasiliauskaite L, Berrens R, Ivanova I, Carrieri C, Reik W, Enright A, et al. Defective germline reprogramming rewires the spermatogonial transcriptome. Nat Struct Mol Biol. 2018;25:394-404 pubmed publisher
Schafler E, Thomas P, Ha S, Wang Y, Bermudez Hernandez K, Tang Z, et al. UXT is required for spermatogenesis in mice. PLoS ONE. 2018;13:e0195747 pubmed publisher
Adams S, Maezawa S, Alavattam K, Abe H, Sakashita A, Shroder M, et al. RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet. 2018;14:e1007233 pubmed publisher
Onizuka S, Iwata T, Park S, Nakai K, Yamato M, Okano T, et al. ZBTB16 as a Downstream Target Gene of Osterix Regulates Osteoblastogenesis of Human Multipotent Mesenchymal Stromal Cells. J Cell Biochem. 2016;117:2423-34 pubmed publisher
Xiang X, Deng L, Zhang J, Zhang X, Lei T, Luan G, et al. A distinct expression pattern of cyclin K in mammalian testes suggests a functional role in spermatogenesis. PLoS ONE. 2014;9:e101539 pubmed publisher
Reichwald K, Jørgensen T, Tougaard P, Skov S. TL1A induces TCR independent IL-6 and TNF-? production and growth of PLZF? leukocytes. PLoS ONE. 2014;9:e85793 pubmed publisher
Wang X, Wang L, Guo S, Bao Y, Ma Y, Yan F, et al. Hypermethylation reduces expression of tumor-suppressor PLZF and regulates proliferation and apoptosis in non-small-cell lung cancers. FASEB J. 2013;27:4194-203 pubmed publisher
Zaade D, Schmitz J, Benke E, Klare S, Seidel K, Kirsch S, et al. Distinct signal transduction pathways downstream of the (P)RR revealed by microarray and ChIP-chip analyses. PLoS ONE. 2013;8:e57674 pubmed publisher
Hou M, Stukenborg J, Nurmio M, Andersson M, Toppari J, Soder O, et al. Ontogenesis of Ap-2? expression in rat testes. Sex Dev. 2011;5:188-96 pubmed publisher
Peppi M, Kujawa S, Sewell W. A corticosteroid-responsive transcription factor, promyelocytic leukemia zinc finger protein, mediates protection of the cochlea from acoustic trauma. J Neurosci. 2011;31:735-41 pubmed publisher
Seidel K, Kirsch S, Lucht K, Zaade D, Reinemund J, Schmitz J, et al. The promyelocytic leukemia zinc finger (PLZF) protein exerts neuroprotective effects in neuronal cells and is dysregulated in experimental stroke. Brain Pathol. 2011;21:31-43 pubmed publisher
Cheung M, Pei J, Pei Y, Jhanwar S, Pass H, Testa J. The promyelocytic leukemia zinc-finger gene, PLZF, is frequently downregulated in malignant mesothelioma cells and contributes to cell survival. Oncogene. 2010;29:1633-40 pubmed publisher
Sadri Ardekani H, Mizrak S, van Daalen S, Korver C, Roepers Gajadien H, Koruji M, et al. Propagation of human spermatogonial stem cells in vitro. JAMA. 2009;302:2127-34 pubmed publisher
Wiebe M, Nichols R, Molitor T, Lindgren J, Traktman P. Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia. Biol Reprod. 2010;82:182-93 pubmed publisher
Barrionuevo F, Georg I, Scherthan H, Lecureuil C, Guillou F, Wegner M, et al. Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009;327:301-12 pubmed publisher
product information
Catalog Number :
sc-28319
Product Name :
PLZF (D-9) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
D-9
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
PLZF
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, IHC(P), ELISA
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA