product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
GATA-1 (N6) Antibody
catalog :
sc-265
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
N6
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 72
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; fig 2c
Grevet J, Lan X, Hamagami N, Edwards C, Sankaranarayanan L, Ji X, et al. Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells. Science. 2018;361:285-290 pubmed publisher
  • western blot; mouse; loading ...; fig 5c
Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 7
Saatcioglu H, Cuevas I, Castrillon D. Control of Oocyte Reawakening by Kit. PLoS Genet. 2016;12:e1006215 pubmed publisher
  • immunocytochemistry; mouse; 8 ug/ml; loading ...; fig 3b
Hoppe P, Schwarzfischer M, Loeffler D, Kokkaliaris K, Hilsenbeck O, Moritz N, et al. Early myeloid lineage choice is not initiated by random PU.1 to GATA1 protein ratios. Nature. 2016;535:299-302 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 1
  • western blot; human; loading ...; fig s1b
  • chromatin immunoprecipitation; mouse; loading ...; fig 5a
Burda P, Vargova J, Curik N, Salek C, Papadopoulos G, Strouboulis J, et al. GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia. PLoS ONE. 2016;11:e0152234 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 7
Minas T, Han J, Javaheri T, Hong S, Schlederer M, SaygideÄŸer Kont Y, et al. YK-4-279 effectively antagonizes EWS-FLI1 induced leukemia in a transgenic mouse model. Oncotarget. 2015;6:37678-94 pubmed publisher
  • western blot; mouse; fig 2b
Yashiro T, Kubo M, Ogawa H, Okumura K, Nishiyama C. PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells. PLoS ONE. 2015;10:e0137699 pubmed publisher
  • chromatin immunoprecipitation; human
Krivega I, Byrnes C, de Vasconcellos J, Lee Y, Kaushal M, Dean A, et al. Inhibition of G9a methyltransferase stimulates fetal hemoglobin production by facilitating LCR/γ-globin looping. Blood. 2015;126:665-72 pubmed publisher
  • chromatin immunoprecipitation; human; fig 4,5,6
Stonestrom A, Hsu S, Jahn K, Huang P, Keller C, Giardine B, et al. Functions of BET proteins in erythroid gene expression. Blood. 2015;125:2825-34 pubmed publisher
  • chromatin immunoprecipitation; mouse
Moriguchi T, Suzuki M, Yu L, Takai J, Ohneda K, Yamamoto M. Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression. Mol Cell Biol. 2015;35:805-15 pubmed publisher
  • western blot; mouse; 1:200
Weston W, Zayas J, Perez R, George J, Jurecic R. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep. 2014;4:5199 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; rat
Ohmori S, Takai J, Ishijima Y, Suzuki M, Moriguchi T, Philipsen S, et al. Regulation of GATA factor expression is distinct between erythroid and mast cell lineages. Mol Cell Biol. 2012;32:4742-55 pubmed publisher
  • western blot; mouse; fig 5c
Yang Y, Ahn Y, Gibbons D, Zang Y, Lin W, Thilaganathan N, et al. The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice. J Clin Invest. 2011;121:1373-85 pubmed publisher
  • chromatin immunoprecipitation; human
Steger D, Lefterova M, Ying L, Stonestrom A, Schupp M, Zhuo D, et al. DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells. Mol Cell Biol. 2008;28:2825-39 pubmed publisher
  • chromatin immunoprecipitation; human
  • EMSA; human
De Gobbi M, Viprakasit V, Hughes J, Fisher C, Buckle V, Ayyub H, et al. A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter. Science. 2006;312:1215-7 pubmed
Ortiz Rivero S, Baquero C, Hernández Cano L, Roldán Etcheverry J, Gutiérrez Herrero S, Fernández Infante C, et al. C3G, through its GEF activity, induces megakaryocytic differentiation and proplatelet formation. Cell Commun Signal. 2018;16:101 pubmed publisher
Shen Y, Bassett M, Gurumurthy A, Nar R, Knudson I, Guy C, et al. Identification of a Novel Enhancer/Chromatin Opening Element Associated with High-Level γ-Globin Gene Expression. Mol Cell Biol. 2018;38: pubmed publisher
Ioannou M, Papageorgiou D, Ogryzko V, Strouboulis J. Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase. BMC Res Notes. 2018;11:390 pubmed publisher
García Carpizo V, Ruiz Llorente S, Sarmentero J, Graña Castro O, Pisano D, Barrero M. CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation. Epigenetics Chromatin. 2018;11:30 pubmed publisher
Tusi B, Wolock S, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, et al. Population snapshots predict early haematopoietic and erythroid hierarchies. Nature. 2018;555:54-60 pubmed publisher
Minkina A, Lindeman R, Gearhart M, Chassot A, Chaboissier M, Ghyselinck N, et al. Retinoic acid signaling is dispensable for somatic development and function in the mammalian ovary. Dev Biol. 2017;424:208-220 pubmed publisher
Mendoza Parra M, Saravaki V, Cholley P, Blum M, Billoré B, Gronemeyer H. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification. F1000Res. 2016;5:54 pubmed publisher
Meinders M, Hoogenboezem M, Scheenstra M, De Cuyper I, Papadopoulos P, Nemeth T, et al. Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment. PLoS ONE. 2016;11:e0154342 pubmed publisher
Gely Pernot A, Hao C, Becker E, Stuparevic I, Kervarrec C, Chalmel F, et al. The epigenetic processes of meiosis in male mice are broadly affected by the widely used herbicide atrazine. BMC Genomics. 2015;16:885 pubmed publisher
Amanatiadou E, Papadopoulos G, Strouboulis J, Vizirianakis I. GATA1 and PU.1 Bind to Ribosomal Protein Genes in Erythroid Cells: Implications for Ribosomopathies. PLoS ONE. 2015;10:e0140077 pubmed publisher
French C, Ye F, Revetta F, Zhang B, Coffey R, Washington M, et al. Linking patient outcome to high throughput protein expression data identifies novel regulators of colorectal adenocarcinoma aggressiveness. F1000Res. 2015;4:99 pubmed publisher
Papageorgiou D, Demmers J, Strouboulis J. NP-40 reduces contamination by endogenous biotinylated carboxylases during purification of biotin tagged nuclear proteins. Protein Expr Purif. 2013;89:80-3 pubmed publisher
Su M, Steiner L, Bogardus H, Mishra T, Schulz V, Hardison R, et al. Identification of biologically relevant enhancers in human erythroid cells. J Biol Chem. 2013;288:8433-44 pubmed publisher
Lavery R, Chassot A, Pauper E, Gregoire E, Klopfenstein M, de Rooij D, et al. Testicular differentiation occurs in absence of R-spondin1 and Sox9 in mouse sex reversals. PLoS Genet. 2012;8:e1003170 pubmed publisher
Costa F, Fedosyuk H, Chazelle A, Neades R, Peterson K. Mi2? is required for ?-globin gene silencing: temporal assembly of a GATA-1-FOG-1-Mi2 repressor complex in ?-YAC transgenic mice. PLoS Genet. 2012;8:e1003155 pubmed publisher
Yocum A, Steiner L, Seidel N, Cline A, Rout E, Lin J, et al. A tissue-specific chromatin loop activates the erythroid ankyrin-1 promoter. Blood. 2012;120:3586-93 pubmed publisher
Kang H, Voleti B, Hajszan T, Rajkowska G, Stockmeier C, Licznerski P, et al. Decreased expression of synapse-related genes and loss of synapses in major depressive disorder. Nat Med. 2012;18:1413-7 pubmed
Baba Y, Maeda K, Yashiro T, Inage E, Kasakura K, Suzuki R, et al. GATA2 is a critical transactivator for the human IL1RL1/ST2 promoter in mast cells/basophils: opposing roles for GATA2 and GATA1 in human IL1RL1/ST2 gene expression. J Biol Chem. 2012;287:32689-96 pubmed
Pellegrino J, Castrillon D, David G. Chromatin associated Sin3A is essential for male germ cell lineage in the mouse. Dev Biol. 2012;369:349-55 pubmed publisher
Thiaville M, Stoeck A, Chen L, Wu R, Magnani L, Oidtman J, et al. Identification of PBX1 target genes in cancer cells by global mapping of PBX1 binding sites. PLoS ONE. 2012;7:e36054 pubmed publisher
Kang Y, Sanalkumar R, O Geen H, Linnemann A, Chang C, Bouhassira E, et al. Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 2012;32:226-39 pubmed publisher
Bonello G, Pham M, Begum K, Sigala J, Sataranatarajan K, Mummidi S. An evolutionarily conserved TNF-alpha-responsive enhancer in the far upstream region of human CCL2 locus influences its gene expression. J Immunol. 2011;186:7025-38 pubmed publisher
Weider K, Bergmann M, Giese S, Guillou F, Failing K, Brehm R. Altered differentiation and clustering of Sertoli cells in transgenic mice showing a Sertoli cell specific knockout of the connexin 43 gene. Differentiation. 2011;82:38-49 pubmed publisher
Zhou J, Pan J, Eckardt S, Leu N, McLaughlin K, Wang P. Nxf3 is expressed in Sertoli cells, but is dispensable for spermatogenesis. Mol Reprod Dev. 2011;78:241-9 pubmed publisher
Amigo J, Yu M, Troadec M, Gwynn B, Cooney J, Lambert A, et al. Identification of distal cis-regulatory elements at mouse mitoferrin loci using zebrafish transgenesis. Mol Cell Biol. 2011;31:1344-56 pubmed publisher
Guo Wei Z, Rui Feng Y, Xiang L, Mitchell W, De Pei L, Chih Chuan L. NF-E2: a novel regulator of alpha-hemoglobin stabilizing protein gene expression. Chin Med Sci J. 2010;25:193-8 pubmed
Cantù C, Grande V, Alborelli I, Cassinelli L, Cantù I, Colzani M, et al. A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells. Nucleic Acids Res. 2011;39:486-501 pubmed publisher
Snow J, Kim J, Currie C, Xu J, Orkin S. Sumoylation regulates interaction of FOG1 with C-terminal-binding protein (CTBP). J Biol Chem. 2010;285:28064-75 pubmed publisher
Kong K, Owens K, Rogers J, Mullenix J, Velu C, Grimes H, et al. MIR-23A microRNA cluster inhibits B-cell development. Exp Hematol. 2010;38:629-640.e1 pubmed publisher
Hernández A, Villegas A, Anguita E. Human promoter mutations unveil Oct-1 and GATA-1 opposite action on Gfi1b regulation. Ann Hematol. 2010;89:759-65 pubmed publisher
Chen R, Chou Y, Lan Y, Huang T, Shen C. Developmental silencing of human zeta-globin gene expression is mediated by the transcriptional repressor RREB1. J Biol Chem. 2010;285:10189-97 pubmed publisher
Miccio A, Wang Y, Hong W, Gregory G, Wang H, Yu X, et al. NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development. EMBO J. 2010;29:442-56 pubmed publisher
Burda P, Curik N, Kokavec J, Basova P, Mikulenkova D, Skoultchi A, et al. PU.1 activation relieves GATA-1-mediated repression of Cebpa and Cbfb during leukemia differentiation. Mol Cancer Res. 2009;7:1693-703 pubmed publisher
Anguita E, Villegas A, Iborra F, Hernández A. GFI1B controls its own expression binding to multiple sites. Haematologica. 2010;95:36-46 pubmed publisher
Amigo J, Ackermann G, Cope J, Yu M, Cooney J, Ma D, et al. The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish. Blood. 2009;114:4654-63 pubmed publisher
Ohneda K, Ohmori S, Ishijima Y, Nakano M, Yamamoto M. Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development. J Biol Chem. 2009;284:30187-99 pubmed publisher
Steiner L, Maksimova Y, Schulz V, Wong C, Raha D, Mahajan M, et al. Chromatin architecture and transcription factor binding regulate expression of erythrocyte membrane protein genes. Mol Cell Biol. 2009;29:5399-412 pubmed publisher
Tallack M, Keys J, Humbert P, Perkins A. EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2. J Biol Chem. 2009;284:20966-74 pubmed publisher
Furuhata A, Murakami M, Ito H, Gao S, Yoshida K, Sobue S, et al. GATA-1 and GATA-2 binding to 3' enhancer of WT1 gene is essential for its transcription in acute leukemia and solid tumor cell lines. Leukemia. 2009;23:1270-7 pubmed publisher
Samuels A, Klinken S, Ingley E. Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin-induced differentiation. Blood. 2009;113:3845-56 pubmed publisher
Di L, Wang L, Zhou G, Wu X, Guo Z, Ke X, et al. Identification of long range regulatory elements of mouse alpha-globin gene cluster by quantitative associated chromatin trap (QACT). J Cell Biochem. 2008;105:301-12 pubmed publisher
Aispuru G, Aguirre M, Aquino Esperanza J, Lettieri C, Juaristi J, Brandan N. Erythroid expansion and survival in response to acute anemia stress: the role of EPO receptor, GATA-1, Bcl-xL and caspase-3. Cell Biol Int. 2008;32:966-78 pubmed publisher
Pina C, May G, Soneji S, Hong D, Enver T. MLLT3 regulates early human erythroid and megakaryocytic cell fate. Cell Stem Cell. 2008;2:264-73 pubmed publisher
Du M, Lv X, Hao D, Zhao G, Wu X, Wu F, et al. MafK/NF-E2 p18 is required for beta-globin genes activation by mediating the proximity of LCR and active beta-globin genes in MEL cell line. Int J Biochem Cell Biol. 2008;40:1481-93 pubmed publisher
Palstra R, Simonis M, Klous P, Brasset E, Eijkelkamp B, de Laat W. Maintenance of long-range DNA interactions after inhibition of ongoing RNA polymerase II transcription. PLoS ONE. 2008;3:e1661 pubmed publisher
Ghinassi B, Verrucci M, Jelicic K, Di Noia A, Migliaccio G, Migliaccio A. Interleukin-3 and erythropoietin cooperate in the regulation of the expression of erythroid-specific transcription factors during erythroid differentiation. Exp Hematol. 2007;35:735-47 pubmed
Kim S, Bardwell V, Zarkower D. Cell type-autonomous and non-autonomous requirements for Dmrt1 in postnatal testis differentiation. Dev Biol. 2007;307:314-27 pubmed
Kim S, Namekawa S, Niswander L, Ward J, Lee J, Bardwell V, et al. A mammal-specific Doublesex homolog associates with male sex chromatin and is required for male meiosis. PLoS Genet. 2007;3:e62 pubmed
Layon M, Ackley C, West R, Lowrey C. Expression of GATA-1 in a non-hematopoietic cell line induces beta-globin locus control region chromatin structure remodeling and an erythroid pattern of gene expression. J Mol Biol. 2007;366:737-44 pubmed
Vernet N, Dennefeld C, Guillou F, Chambon P, Ghyselinck N, Mark M. Prepubertal testis development relies on retinoic acid but not rexinoid receptors in Sertoli cells. EMBO J. 2006;25:5816-25 pubmed
Keys J, Tallack M, Hodge D, Cridland S, David R, Perkins A. Genomic organisation and regulation of murine alpha haemoglobin stabilising protein by erythroid Kruppel-like factor. Br J Haematol. 2007;136:150-7 pubmed
Karur V, Lowell C, Besmer P, Agosti V, Wojchowski D. Lyn kinase promotes erythroblast expansion and late-stage development. Blood. 2006;108:1524-32 pubmed
Suh H, Gooya J, Renn K, Friedman A, Johnson P, Keller J. C/EBPalpha determines hematopoietic cell fate in multipotential progenitor cells by inhibiting erythroid differentiation and inducing myeloid differentiation. Blood. 2006;107:4308-16 pubmed
Gutierrez L, Lindeboom F, Ferreira R, Drissen R, Grosveld F, Whyatt D, et al. A hanging drop culture method to study terminal erythroid differentiation. Exp Hematol. 2005;33:1083-91 pubmed
Hong W, Nakazawa M, Chen Y, Kori R, Vakoc C, Rakowski C, et al. FOG-1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA-1. EMBO J. 2005;24:2367-78 pubmed
Ingley E, McCarthy D, Pore J, Sarna M, Adenan A, Wright M, et al. Lyn deficiency reduces GATA-1, EKLF and STAT5, and induces extramedullary stress erythropoiesis. Oncogene. 2005;24:336-43 pubmed
Martowicz M, Grass J, Boyer M, Guend H, Bresnick E. Dynamic GATA factor interplay at a multicomponent regulatory region of the GATA-2 locus. J Biol Chem. 2005;280:1724-32 pubmed
product information
Product Name :
GATA-1 (N6) Antibody
Gene Name :
GATA1
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-265
Type :
Monoclonal
Host :
rat monoclonal
Antigen :
GATA-1
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P)
Epitope :
FL (m)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA