product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
acetylated α Tubulin (6-11B-1) Antibody
catalog :
sc-23950
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6-11B-1
reactivity :
human, mouse, rat, Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 69
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; rat; 1:10,000; loading ...; fig 2b
  • western blot; rat; 1:10,000; loading ...; fig 2a
Chen L, Hu Q, Liu H, Zhao Y, Chan S, Wang J. Nogo-A Induced Polymerization of Microtubule Is Involved in the Inflammatory Heat Hyperalgesia in Rat Dorsal Root Ganglion Neurons. Int J Mol Sci. 2021;22: pubmed publisher
  • immunocytochemistry; human; 1:100; fig 7c
Lamers M, Mykytyn A, Breugem T, Wang Y, Wu D, Riesebosch S, et al. Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation. elife. 2021;10: pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 1s1a
Mykytyn A, Breugem T, Riesebosch S, Schipper D, van den Doel P, Rottier R, et al. SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site. elife. 2021;10: pubmed publisher
  • immunocytochemistry; human; 1:160; loading ...; fig 3a
Bozal Basterra L, Gonzalez Santamarta M, Muratore V, Bermejo Arteagabeitia A, Da Fonseca C, Barroso Gomila O, et al. LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome. elife. 2020;9: pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 3d
  • western blot; rat; loading ...; fig 3e, s4b
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3a
  • immunocytochemistry; human; 1:200; loading ...; fig 2a
  • western blot; human; loading ...; fig 4e, 5a
Li S, Wei Z, Li G, Zhang Q, Niu S, Xu D, et al. Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics. 2020;10:1719-1732 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2g
Douanne T, André Grégoire G, Thys A, Trillet K, Gavard J, Bidere N. CYLD Regulates Centriolar Satellites Proteostasis by Counteracting the E3 Ligase MIB1. Cell Rep. 2019;27:1657-1665.e4 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 1c
Sachs N, Papaspyropoulos A, Zomer van Ommen D, Heo I, Böttinger L, Klay D, et al. Long-term expanding human airway organoids for disease modeling. EMBO J. 2019;38: pubmed publisher
  • western blot; mouse; loading ...; fig 6a
  • immunocytochemistry; human; loading ...; fig 6g
  • western blot; human; loading ...; fig 6e
Li W, Yue F, Dai Y, Shi B, Xu G, Jiang X, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ. 2018;: pubmed publisher
  • western blot; mouse; fig 6g
Beauchemin H, Shooshtarizadeh P, Vadnais C, Vassen L, Pastore Y, Moroy T. Gfi1b controls integrin signaling-dependent cytoskeleton dynamics and organization in megakaryocytes. Haematologica. 2017;102:484-497 pubmed publisher
  • immunocytochemistry; rat; loading ...; fig 2a
Xiaojun W, Yan L, Hong X, Xianghong Z, Shifeng L, Dingjie X, et al. Acetylated ?-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica. Sci Rep. 2016;6:32257 pubmed publisher
  • western blot; mouse; fig 7
Furukawa Y, Tanemura K, Igarashi K, Ideta Otsuka M, Aisaki K, Kitajima S, et al. Learning and Memory Deficits in Male Adult Mice Treated with a Benzodiazepine Sleep-Inducing Drug during the Juvenile Period. Front Neurosci. 2016;10:339 pubmed publisher
  • immunohistochemistry; Xenopus laevis; 1:500; fig s1
Chu C, Ossipova O, Ioannou A, Sokol S. Prickle3 synergizes with Wtip to regulate basal body organization and cilia growth. Sci Rep. 2016;6:24104 pubmed publisher
  • western blot; mouse; 1 ug/ml; fig 5
Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
  • western blot; mouse; fig 1
  • western blot; human; fig 1
M L, P P, T K, M P, E S, J P, et al. Essential role of HDAC6 in the regulation of PD-L1 in melanoma. Mol Oncol. 2016;10:735-750 pubmed publisher
  • western blot; human; fig 3
Seidel C, Schnekenburger M, Mazumder A, Teiten M, Kirsch G, Dicato M, et al. 4-Hydroxybenzoic acid derivatives as HDAC6-specific inhibitors modulating microtubular structure and HSP90α chaperone activity against prostate cancer. Biochem Pharmacol. 2016;99:31-52 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 5a
Ragot A, Pietropaolo S, Vincent J, Delage P, Zhang H, Allinquant B, et al. Genetic deletion of the Histone Deacetylase 6 exacerbates selected behavioral deficits in the R6/1 mouse model for Huntington's disease. Brain Behav. 2015;5:e00361 pubmed publisher
  • western blot; mouse
Woan K, Lienlaf M, Perez Villaroel P, Lee C, Cheng F, Knox T, et al. Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation. Mol Oncol. 2015;9:1447-1457 pubmed publisher
  • immunocytochemistry; human; 1:1000
Bailey J, Fields A, Cheng K, Lee A, Wagenaar E, Lagrois R, et al. WD repeat-containing protein 5 (WDR5) localizes to the midbody and regulates abscission. J Biol Chem. 2015;290:8987-9001 pubmed publisher
  • western blot; mouse; fig 4
Cheng F, Lienlaf M, Wang H, Perez Villarroel P, Lee C, Woan K, et al. A novel role for histone deacetylase 6 in the regulation of the tolerogenic STAT3/IL-10 pathway in APCs. J Immunol. 2014;193:2850-62 pubmed publisher
  • western blot; human; 1:250
Erdozain A, Morentin B, Bedford L, King E, Tooth D, Brewer C, et al. Alcohol-related brain damage in humans. PLoS ONE. 2014;9:e93586 pubmed publisher
  • immunocytochemistry; human
Klinger M, Wang W, Kuhns S, Bärenz F, Dräger Meurer S, Pereira G, et al. The novel centriolar satellite protein SSX2IP targets Cep290 to the ciliary transition zone. Mol Biol Cell. 2014;25:495-507 pubmed publisher
  • western blot; mouse; 1:2000
Schreiner A, Durry S, Aida T, Stock M, Ruther U, Tanaka K, et al. Laminar and subcellular heterogeneity of GLAST and GLT-1 immunoreactivity in the developing postnatal mouse hippocampus. J Comp Neurol. 2014;522:204-24 pubmed publisher
Liu S, Casey Clyde T, Cho N, Swinderman J, Pekmezci M, Dougherty M, et al. Epigenetic reprogramming shapes the cellular landscape of schwannoma. Nat Commun. 2024;15:476 pubmed publisher
Wang Y, Risteski P, Yang Y, Chen H, Droby G, Walens A, et al. The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation. Nucleic Acids Res. 2023;51:10568-10589 pubmed publisher
Boegholm N, Petriman N, Loureiro L xf3 pez M, Wang J, Vela M, Liu B, et al. The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport. EMBO J. 2023;42:e111807 pubmed publisher
Shim S, Goyal R, Panoutsopoulos A, Balashova O, Lee D, Borodinsky L. Calcium dynamics at the neural cell primary cilium regulate Hedgehog signaling-dependent neurogenesis in the embryonic neural tube. Proc Natl Acad Sci U S A. 2023;120:e2220037120 pubmed publisher
Lyu J, Li Z, Roberts J, Qi Y, Xiong J. The short-chain fatty acid acetate coordinates with CD30 to modulate T-cell survival. Mol Biol Cell. 2023;34:br11 pubmed publisher
Lee J, Jeong S, Li Z, Kim H, Kim H, Yoo M, et al. Development of a screening platform to discover natural products active against SARS-CoV-2 infection using lung organoid models. Biomater Res. 2023;27:18 pubmed publisher
Czerny C, Borschel A, Cai M, Otto M, Hoyer Fender S. FOXA1 is a transcriptional activator of Odf2/Cenexin and regulates primary ciliation. Sci Rep. 2022;12:21468 pubmed publisher
Mouron S, Bueno M, Lluch A, Manso L, Calvo I, Cortes J, et al. Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A. Nat Commun. 2022;13:7529 pubmed publisher
Tang L, Spezia M, Chen T, Shin J, Wang F, Stappenbeck F, et al. Oxysterol derivatives Oxy186 and Oxy210 inhibit WNT signaling in non-small cell lung cancer. Cell Biosci. 2022;12:119 pubmed publisher
Miyake K, Takano N, Kazama H, Kikuchi H, Hiramoto M, Tsukahara K, et al. Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. Int J Oncol. 2022;60: pubmed publisher
Okubo Y, Ohtake F, Igarashi K, Yasuhiko Y, Hirabayashi Y, Saga Y, et al. Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways. Development. 2021;148: pubmed publisher
Rasamizafy S, Delsert C, Rabeharivelo G, Cau J, Morin N, Van Dijk J. Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis. Cells. 2021;10: pubmed publisher
Roncato F, Regev O, Yadav S, Alon R. Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells. Cell Adh Migr. 2021;15:166-179 pubmed publisher
Findakly S, Daggubati V, Garcia G, LaStella S, Choudhury A, Tran C, et al. Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway. J Cell Biol. 2021;220: pubmed publisher
Schotthöfer S, Bohrmann J. Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange. BMC Dev Biol. 2020;20:15 pubmed publisher
Schotthöfer S, Bohrmann J. Bioelectrical and cytoskeletal patterns correlate with altered axial polarity in the follicular epithelium of the Drosophila mutant gurken. BMC Dev Biol. 2020;20:5 pubmed publisher
Sarcinelli C, Dragic H, Piecyk M, Barbet V, Duret C, Barthelaix A, et al. ATF4-Dependent NRF2 Transcriptional Regulation Promotes Antioxidant Protection during Endoplasmic Reticulum Stress. Cancers (Basel). 2020;12: pubmed publisher
Weiß I, Bohrmann J. Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary. BMC Dev Biol. 2019;19:22 pubmed publisher
Rozario T, Quinn E, Wang J, Davis R, Newmark P. Region-specific regulation of stem cell-driven regeneration in tapeworms. elife. 2019;8: pubmed publisher
Menden H, Xia S, Mabry S, Noel MacDonnell J, Rajasingh J, Qing Ye S, et al. Histone Deacetylase 6 Regulates Endothelial MyD88-dependent canonical TLR signaling, Lung inflammation, and Alveolar Remodeling in the developing lung. Am J Physiol Lung Cell Mol Physiol. 2019;: pubmed publisher
Adamakis I, Panteris E, Eleftheriou E. Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum. Biomolecules. 2019;9: pubmed publisher
Kiseleva A, Korobeynikov V, Nikonova A, Zhang P, Makhov P, Deneka A, et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs. Clin Cancer Res. 2019;: pubmed publisher
Zhang P, Kiseleva A, Korobeynikov V, Liu H, Einarson M, Golemis E. Microscopy-Based Automated Live Cell Screening for Small Molecules That Affect Ciliation. Front Genet. 2019;10:75 pubmed publisher
Denu R, Sass M, Johnson J, Potts G, Choudhary A, Coon J, et al. Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131). J Biol Chem. 2019;294:6531-6549 pubmed publisher
Van Dijk J, Bompard G, Cau J, Kunishima S, Rabeharivelo G, Mateos Langerak J, et al. Microtubule polyglutamylation and acetylation drive microtubule dynamics critical for platelet formation. BMC Biol. 2018;16:116 pubmed publisher
Labisso W, Raulin A, Nwidu L, Kocon A, Wayne D, Erdozain A, et al. The Loss of α- and β-Tubulin Proteins Are a Pathological Hallmark of Chronic Alcohol Consumption and Natural Brain Ageing. Brain Sci. 2018;8: pubmed publisher
Chen X, Fang F, Florio J, Rockenstein E, Masliah E, Mobley W, et al. T-complex protein 1-ring complex enhances retrograde axonal transport by modulating tau phosphorylation. Traffic. 2018;19:840-853 pubmed publisher
Pathak G, Shah R, Kennedy B, Murphy J, Clements D, Konda P, et al. RTN4 Knockdown Dysregulates the AKT Pathway, Destabilizes the Cytoskeleton, and Enhances Paclitaxel-Induced Cytotoxicity in Cancers. Mol Ther. 2018;26:2019-2033 pubmed publisher
Romanick S, Ulrich C, Schlauch K, Hostler A, Payne J, Woolsey R, et al. Obesity-mediated regulation of cardiac protein acetylation: parallel analysis of total and acetylated proteins via TMT-tagged mass spectrometry. Biosci Rep. 2018;38: pubmed publisher
Price M, Case A, Pavlik J, Devasure J, Wyatt T, Zimmerman M, et al. S-nitrosation of protein phosphatase 1 mediates alcohol-induced ciliary dysfunction. Sci Rep. 2018;8:9701 pubmed publisher
Kannan M, Bayam E, Wagner C, Rinaldi B, Kretz P, Tilly P, et al. WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy. Proc Natl Acad Sci U S A. 2017;114:E9308-E9317 pubmed publisher
Yanda M, Liu Q, Cebotaru V, Guggino W, Cebotaru L. Histone deacetylase 6 inhibition reduces cysts by decreasing cAMP and Ca2+ in knock-out mouse models of polycystic kidney disease. J Biol Chem. 2017;292:17897-17908 pubmed publisher
Jiang P, Li Y, Poleshko A, Medvedeva V, Baulina N, Zhang Y, et al. The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. EBioMedicine. 2017;22:28-43 pubmed publisher
Salemi L, Maitland M, Yefet E, Schild Poulter C. Inhibition of HDAC6 activity through interaction with RanBPM and its associated CTLH complex. BMC Cancer. 2017;17:460 pubmed publisher
Li X, Thome S, Ma X, Amrute Nayak M, Finigan A, Kitt L, et al. MARK4 regulates NLRP3 positioning and inflammasome activation through a microtubule-dependent mechanism. Nat Commun. 2017;8:15986 pubmed publisher
Geng F, Cheng X, Wu X, Yoo J, Cheng C, Guo J, et al. Inhibition of SOAT1 Suppresses Glioblastoma Growth via Blocking SREBP-1-Mediated Lipogenesis. Clin Cancer Res. 2016;22:5337-5348 pubmed
Sun H, He L, Wu H, Pan F, Wu X, Zhao J, et al. The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation. Oncogene. 2017;36:194-207 pubmed publisher
Chen B, Li R, Yan N, Chen G, Qian W, Jiang H, et al. Astragaloside IV controls collagen reduction in photoaging skin by improving transforming growth factor-β/Smad signaling suppression and inhibiting matrix metalloproteinase-1. Mol Med Rep. 2015;11:3344-8 pubmed publisher
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McGowan S, McCoy D. Platelet-derived growth factor-A and sonic hedgehog signaling direct lung fibroblast precursors during alveolar septal formation. Am J Physiol Lung Cell Mol Physiol. 2013;305:L229-39 pubmed publisher
Giannoutsou E, Galatis B, Zachariadis M, Apostolakos P. Formation of an endoplasmic reticulum ring associated with acetylated microtubules in the angiosperm preprophase band. Cytoskeleton (Hoboken). 2012;69:252-65 pubmed publisher
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product information
Catalog Number :
sc-23950
Product Name :
acetylated α Tubulin (6-11B-1) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
6-11B-1
Isotype :
IgG2b
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
alpha Tubulin
Specificity :
mammalian species, zebrafish, Drosophila, Xenopus
Uses :
WB, IP, IF, IHC(P)
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA