product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
T-bet (4B10) Antibody
catalog :
sc-21749
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4B10
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, western blot knockout validation
citations: 27
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:100; loading ...; fig 1b
Hoegl S, Ehrentraut H, Brodsky K, Victorino F, Golden Mason L, Eltzschig H, et al. NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury. J Leukoc Biol. 2017;101:471-480 pubmed publisher
  • flow cytometry; mouse; fig s3b
Knudson K, Pritzl C, Saxena V, Altman A, Daniels M, Teixeiro E. NFκB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality. Proc Natl Acad Sci U S A. 2017;114:E1659-E1667 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2f
Allison K, Sajti E, Collier J, Gosselin D, Troutman T, Stone E, et al. Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells. elife. 2016;5: pubmed publisher
  • western blot; mouse; 1:200; fig 4
Castiglioni A, Corna G, Rigamonti E, Basso V, Vezzoli M, Monno A, et al. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration. PLoS ONE. 2015;10:e0128094 pubmed publisher
  • flow cytometry; mouse; fig 8
Pei B, Zhao M, Miller B, Véla J, Bruinsma M, Virgin H, et al. Invariant NKT cells require autophagy to coordinate proliferation and survival signals during differentiation. J Immunol. 2015;194:5872-84 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4f
  • western blot; human; loading ...; fig 4c
Chen H, Huang W, Yang L, Lin C. The PTEN-AKT-mTOR/RICTOR Pathway in Nasal Natural Killer Cell Lymphoma Is Activated by miR-494-3p via PTEN But Inhibited by miR-142-3p via RICTOR. Am J Pathol. 2015;185:1487-99 pubmed publisher
  • immunohistochemistry; human; 1:100
Perino G, Ricciardi B, Jerabek S, Martignoni G, Wilner G, Maass D, et al. Implant based differences in adverse local tissue reaction in failed total hip arthroplasties: a morphological and immunohistochemical study. BMC Clin Pathol. 2014;14:39 pubmed publisher
  • chromatin immunoprecipitation; mouse
Collier S, Henderson M, Tossberg J, Aune T. Regulation of the Th1 genomic locus from Ifng through Tmevpg1 by T-bet. J Immunol. 2014;193:3959-65 pubmed publisher
  • western blot; mouse
Tong Q, He S, Xie F, Mochizuki K, Liu Y, Mochizuki I, et al. Ezh2 regulates transcriptional and posttranslational expression of T-bet and promotes Th1 cell responses mediating aplastic anemia in mice. J Immunol. 2014;192:5012-22 pubmed publisher
  • western blot; mouse; fig 1
Oestreich K, Mohn S, Weinmann A. Molecular mechanisms that control the expression and activity of Bcl-6 in TH1 cells to regulate flexibility with a TFH-like gene profile. Nat Immunol. 2012;13:405-11 pubmed publisher
Manni M, Gupta S, Ricker E, Chinenov Y, Park S, Shi M, et al. Regulation of age-associated B cells by IRF5 in systemic autoimmunity. Nat Immunol. 2018;19:407-419 pubmed publisher
Muroyama Y, Baba A, Kitagawa M, Saito T. Olfactory Sensory Neurons Control Dendritic Complexity of Mitral Cells via Notch Signaling. PLoS Genet. 2016;12:e1006514 pubmed publisher
Caramori G, Lasagna L, Casalini A, Adcock I, Casolari P, Contoli M, et al. Immune response to Mycobacterium tuberculosis infection in the parietal pleura of patients with tuberculous pleurisy. PLoS ONE. 2011;6:e22637 pubmed publisher
Hwang S, Kim K, Lee G. Defective GATA-3 expression in Th2 LCR-deficient mice. Biochem Biophys Res Commun. 2011;410:866-71 pubmed publisher
Farley S, Wood L, Iordanov M. An epidermotypic model of interface dermatitis reveals individual functions of fas ligand and gamma interferon in hypergranulosis, cytoid body formation, and gene expression. Am J Dermatopathol. 2011;33:244-50 pubmed publisher
Placek K, Gasparian S, Coffre M, Maiella S, Sechet E, Bianchi E, et al. Integration of distinct intracellular signaling pathways at distal regulatory elements directs T-bet expression in human CD4+ T cells. J Immunol. 2009;183:7743-51 pubmed publisher
Ong C, Sedy J, Murphy K, Kopan R. Notch and presenilin regulate cellular expansion and cytokine secretion but cannot instruct Th1/Th2 fate acquisition. PLoS ONE. 2008;3:e2823 pubmed publisher
Nagamachi M, Sakata D, Kabashima K, Furuyashiki T, Murata T, Segi Nishida E, et al. Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1. J Exp Med. 2007;204:2865-74 pubmed
Daniel C, Sartory N, Zahn N, Radeke H, Stein J. Immune modulatory treatment of trinitrobenzene sulfonic acid colitis with calcitriol is associated with a change of a T helper (Th) 1/Th17 to a Th2 and regulatory T cell profile. J Pharmacol Exp Ther. 2008;324:23-33 pubmed
Ward F, Hall A, Cairns L, Leggat A, Urbaniak S, Vickers M, et al. Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia. Blood. 2008;111:680-7 pubmed
Naoe Y, Setoguchi R, Akiyama K, Muroi S, Kuroda M, Hatam F, et al. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the Il4 silencer. J Exp Med. 2007;204:1749-55 pubmed
Lund R, Löytömäki M, Naumanen T, Dixon C, Chen Z, Ahlfors H, et al. Genome-wide identification of novel genes involved in early Th1 and Th2 cell differentiation. J Immunol. 2007;178:3648-60 pubmed
Alcaide P, Jones T, Lord G, Glimcher L, Hallgren J, Arinobu Y, et al. Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue. J Exp Med. 2007;204:431-9 pubmed
Jankovic D, Kullberg M, Feng C, Goldszmid R, Collazo C, Wilson M, et al. Conventional T-bet(+)Foxp3(-) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection. J Exp Med. 2007;204:273-83 pubmed
Daniel C, Radeke H, Sartory N, Zahn N, Zuegel U, Steinmeyer A, et al. The new low calcemic vitamin D analog 22-ene-25-oxa-vitamin D prominently ameliorates T helper cell type 1-mediated colitis in mice. J Pharmacol Exp Ther. 2006;319:622-31 pubmed
Zhang F, Boothby M. T helper type 1-specific Brg1 recruitment and remodeling of nucleosomes positioned at the IFN-gamma promoter are Stat4 dependent. J Exp Med. 2006;203:1493-505 pubmed
Dunn S, Youssef S, Goldstein M, Prod homme T, Weber M, Zamvil S, et al. Isoprenoids determine Th1/Th2 fate in pathogenic T cells, providing a mechanism of modulation of autoimmunity by atorvastatin. J Exp Med. 2006;203:401-12 pubmed
product information
Catalog Number :
sc-21749
Product Name :
T-bet (4B10) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
4B10
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
T-bet
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, FCM, ELISA
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA