product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
LAMP-1 (H4A3) Antibody
catalog :
sc-20011
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H4A3
reactivity :
fission yeast, human, mouse, brewer's yeast
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 109
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:200; loading ...; fig s5f
Pan R, Yu Y, Zhu H, Zhang W, Qin Y, Ye L, et al. RSPO2 promotes progression of ovarian cancer through dual receptor-mediated FAK/Src signaling activation. iScience. 2022;25:105184 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig s6b
Zhu J, Pittman S, Dhavale D, French R, Patterson J, Kaleelurrrahuman M, et al. VCP suppresses proteopathic seeding in neurons. Mol Neurodegener. 2022;17:30 pubmed publisher
  • immunocytochemistry; human; 1:400; loading ...
Soldati C, Lopez Fabuel I, Wanderlingh L, García Macia M, Monfregola J, Esposito A, et al. Repurposing of tamoxifen ameliorates CLN3 and CLN7 disease phenotype. EMBO Mol Med. 2021;13:e13742 pubmed publisher
  • western blot; human; 1:200; loading ...; fig 6f
Kasahara Y, Osuka S, Takasaki N, Bayasula -, Koya Y, Nakanishi N, et al. Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells. Cell Death Discov. 2021;7:186 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3a
Singh M, Zangoui P, Yamanaka Y, Kenney L. Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors. elife. 2021;10: pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig 4i
Tamargo Gómez I, Martínez García G, Suarez M, Rey V, Fueyo A, Codina Martínez H, et al. ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration. Cell Death Differ. 2021;: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 6e
Ryan B, Bengoa Vergniory N, Williamson M, Kirkiz E, Roberts R, Corda G, et al. REST protects dopaminergic neurons from mitochondrial and α-synuclein oligomer pathology in an alpha synuclein overexpressing BAC-transgenic mouse model. J Neurosci. 2021;: pubmed publisher
  • western blot; mouse; 1:5000; loading ...; fig 4a
Lanzillotta C, Zuliani I, Vasavda C, Snyder S, Paul B, Perluigi M, et al. BVR-A Deficiency Leads to Autophagy Impairment through the Dysregulation of AMPK/mTOR Axis in the Brain-Implications for Neurodegeneration. Antioxidants (Basel). 2020;9: pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 5a
Adapala N, Swarnkar G, Arra M, Shen J, Mbalaviele G, Ke K, et al. Inflammatory osteolysis is regulated by site-specific ISGylation of the scaffold protein NEMO. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 4a
Pietila M, Sahgal P, Peuhu E, Jäntti N, Paatero I, Närvä E, et al. SORLA regulates endosomal trafficking and oncogenic fitness of HER2. Nat Commun. 2019;10:2340 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig s2
Sahgal P, Alanko J, Icha J, Paatero I, Hamidi H, Arjonen A, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. J Cell Sci. 2019;132: pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 7b
Saito T, Kuma A, Sugiura Y, Ichimura Y, Obata M, Kitamura H, et al. Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nat Commun. 2019;10:1567 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 5a
Sapmaz A, Berlin I, Bos E, Wijdeven R, Janssen H, Konietzny R, et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun. 2019;10:1454 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2c
Zhang J, He J, Johnson J, Rahman F, Gavathiotis E, Cuervo A, et al. Chaperone-Mediated Autophagy Upregulation Rescues Megalin Expression and Localization in Cystinotic Proximal Tubule Cells. Front Endocrinol (Lausanne). 2019;10:21 pubmed publisher
  • western blot; human; loading ...; fig 2f
Wang D, Xu Q, Yuan Q, Jia M, Niu H, Liu X, et al. Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene. 2019;38:3458-3474 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Walwyn Brown K, Guldevall K, Saeed M, Pende D, Önfelt B, MacDonald A, et al. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1. J Immunol. 2018;201:2028-2041 pubmed publisher
  • western blot; human; loading ...; fig 5a
Wang W, Xia Z, Farre J, Subramani S. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy. 2018;14:1574-1585 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s2c
Zhao Y, Wu X, Li X, Jiang L, Gui X, Liu Y, et al. TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function. Neuron. 2018;97:1023-1031.e7 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1f
Wei J, Xu H, Meng W. Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk. FEBS Lett. 2017;591:2379-2393 pubmed publisher
  • western blot; human; loading ...; fig 1c
Wang W, Xia Z, Farré J, Subramani S. TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import. J Cell Biol. 2017;216:2843-2858 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 4a
Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig s4
Geraets R, Langin L, Cain J, Parker C, Beraldi R, Kovács A, et al. A tailored mouse model of CLN2 disease: A nonsense mutant for testing personalized therapies. PLoS ONE. 2017;12:e0176526 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s3a
Allison R, Edgar J, Pearson G, Rizo T, Newton T, Günther S, et al. Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia. J Cell Biol. 2017;216:1337-1355 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 3c
Kang H, Park J, Choi K, Kim Y, Choi H, Jung C, et al. Chemical screening identifies ATM as a target for alleviating senescence. Nat Chem Biol. 2017;13:616-623 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1b
  • immunocytochemistry; mouse
Hubber A, Kubori T, Coban C, Matsuzawa T, Ogawa M, Kawabata T, et al. Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis. Sci Rep. 2017;7:44795 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 3B
Miles A, Burr S, Grice G, Nathan J. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels. elife. 2017;6: pubmed publisher
  • immunocytochemistry; human; fig 4a1
Zimmermann Meisse G, Prevost G, Jover E. Above and beyond C5a Receptor Targeting by Staphylococcal Leucotoxins: Retrograde Transport of Panton-Valentine Leucocidin and ?-Hemolysin. Toxins (Basel). 2017;9: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1d
Nakajima S, Aikawa C, Nozawa T, Minowa Nozawa A, Toh H, Nakagawa I. Bcl-xL Affects Group A Streptococcus-Induced Autophagy Directly, by Inhibiting Fusion between Autophagosomes and Lysosomes, and Indirectly, by Inhibiting Bacterial Internalization via Interaction with Beclin 1-UVRAG. PLoS ONE. 2017;12:e0170138 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 1h
Muranen T, Iwanicki M, Curry N, Hwang J, DuBois C, Coloff J, et al. Starved epithelial cells uptake extracellular matrix for survival. Nat Commun. 2017;8:13989 pubmed publisher
  • immunocytochemistry; mouse; 1:100
  • western blot; mouse; 1:200; loading ...; fig s5a
Calamita P, Miluzio A, Russo A, Pesce E, Ricciardi S, Khanim F, et al. SBDS-Deficient Cells Have an Altered Homeostatic Equilibrium due to Translational Inefficiency Which Explains their Reduced Fitness and Provides a Logical Framework for Intervention. PLoS Genet. 2017;13:e1006552 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 4c
Żarska M, Novotný F, Havel F, Sramek M, Babelova A, Benada O, et al. Two-Step Mechanism of Cellular Uptake of Cationic Gold Nanoparticles Modified by (16-Mercaptohexadecyl)trimethylammonium Bromide. Bioconjug Chem. 2016;27:2558-2574 pubmed
  • western blot; human; 1:2000; loading ...; fig 5d
Fernández B, Fdez E, Gomez Suaga P, Gil F, Molina Villalba I, Ferrer I, et al. Iron overload causes endolysosomal deficits modulated by NAADP-regulated 2-pore channels and RAB7A. Autophagy. 2016;12:1487-506 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6b
Xie N, Yuan K, Zhou L, Wang K, Chen H, Lei Y, et al. PRKAA/AMPK restricts HBV replication through promotion of autophagic degradation. Autophagy. 2016;12:1507-20 pubmed publisher
  • western blot; brewer's yeast; 1:1000; fig s4
Andersson A, Andersson B, Lorell C, Raffetseder J, Larsson M, Blomgran R. Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages. Sci Rep. 2016;6:28171 pubmed publisher
  • immunocytochemistry; human
  • immunocytochemistry; mouse
Nakamura T, Furukawa A, Uchida K, Ogawa T, Tamura T, Sakonishi D, et al. Autophagy Induced by Intracellular Infection of Propionibacterium acnes. PLoS ONE. 2016;11:e0156298 pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 4a
Scharn C, Collins A, Nair V, Stamm C, MARCIANO D, Graviss E, et al. Heme Oxygenase-1 Regulates Inflammation and Mycobacterial Survival in Human Macrophages during Mycobacterium tuberculosis Infection. J Immunol. 2016;196:4641-9 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 4
Karvela M, Baquero P, Kuntz E, Mukhopadhyay A, Mitchell R, Allan E, et al. ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells. Autophagy. 2016;12:936-48 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; 1:1000; fig 2
Gschweitl M, Ulbricht A, Barnes C, Enchev R, Stoffel Studer I, Meyer Schaller N, et al. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes. elife. 2016;5:e13841 pubmed publisher
  • western blot; human; 1:200; tbl 2
Piras A, Collin L, Grüninger F, Graff C, Rönnbäck A. Autophagic and lysosomal defects in human tauopathies: analysis of post-mortem brain from patients with familial Alzheimer disease, corticobasal degeneration and progressive supranuclear palsy. Acta Neuropathol Commun. 2016;4:22 pubmed publisher
  • immunocytochemistry; human; fig 1
Oda S, Nozawa T, Nozawa Minowa A, Tanaka M, Aikawa C, Harada H, et al. Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. PLoS ONE. 2016;11:e0147061 pubmed publisher
  • immunocytochemistry; human
Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T. APP controls the formation of PI(3,5)P(2) vesicles through its binding of the PIKfyve complex. Cell Mol Life Sci. 2016;73:393-408 pubmed publisher
  • immunocytochemistry; human; fig 3
  • western blot; human
Su X, Yu Y, Zhong Y, Giannopoulou E, Hu X, Liu H, et al. Interferon-γ regulates cellular metabolism and mRNA translation to potentiate macrophage activation. Nat Immunol. 2015;16:838-849 pubmed publisher
  • immunocytochemistry; human; fig s4b
Munson M, Allen G, Toth R, Campbell D, Lucocq J, Ganley I. mTOR activates the VPS34-UVRAG complex to regulate autolysosomal tubulation and cell survival. EMBO J. 2015;34:2272-90 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 6
Okatsu K, Koyano F, Kimura M, Kosako H, Saeki Y, Tanaka K, et al. Phosphorylated ubiquitin chain is the genuine Parkin receptor. J Cell Biol. 2015;209:111-28 pubmed publisher
  • western blot; human; fig 3
Gotink K, Rovithi M, de Haas R, Honeywell R, Dekker H, Poel D, et al. Cross-resistance to clinically used tyrosine kinase inhibitors sunitinib, sorafenib and pazopanib. Cell Oncol (Dordr). 2015;38:119-29 pubmed publisher
  • immunocytochemistry; fission yeast; fig 2
Kira S, Tabata K, Shirahama Noda K, Nozoe A, Yoshimori T, Noda T. Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy. Autophagy. 2014;10:1565-78 pubmed publisher
  • immunohistochemistry; human; 1:100
Hagel C, Krasemann S, Löffler J, Puschel K, Magnus T, Glatzel M. Upregulation of Shiga toxin receptor CD77/Gb3 and interleukin-1? expression in the brain of EHEC patients with hemolytic uremic syndrome and neurologic symptoms. Brain Pathol. 2015;25:146-56 pubmed publisher
  • immunocytochemistry; human; fig 3
Huttunen M, Waris M, Kajander R, Hyypia T, Marjomaki V. Coxsackievirus A9 infects cells via nonacidic multivesicular bodies. J Virol. 2014;88:5138-51 pubmed publisher
  • flow cytometry; human
Cardoso C, Groth Pedersen L, Høyer Hansen M, Kirkegaard T, Corcelle E, Andersen J, et al. Depletion of kinesin 5B affects lysosomal distribution and stability and induces peri-nuclear accumulation of autophagosomes in cancer cells. PLoS ONE. 2009;4:e4424 pubmed publisher
  • immunocytochemistry; human
Garver W, Jelinek D, Francis G, Murphy B. The Niemann-Pick C1 gene is downregulated by feedback inhibition of the SREBP pathway in human fibroblasts. J Lipid Res. 2008;49:1090-102 pubmed publisher
Basbous S, Dif L, Dantzer C, Di Tommaso S, Dupuy J, Bioulac Sage P, et al. Loss of RND3/RHOE controls entosis through LAMP1 expression in hepatocellular carcinoma. Cell Death Dis. 2024;15:46 pubmed publisher
Krogsaeter E, McKetney J, Marquez A, Cakir Z, Stevenson E, Jang G, et al. Lysosomal proteomics reveals mechanisms of neuronal apoE4associated lysosomal dysfunction. bioRxiv. 2023;: pubmed publisher
Rose K, Jepson T, Shukla S, Maya Romero A, Kampmann M, Xu K, et al. Tau fibrils induce nanoscale membrane damage and nucleate cytosolic tau at lysosomes. bioRxiv. 2023;: pubmed publisher
Coppola V, Marino I, Warnken U, Falchi M, Pasquini L, Biffoni M, et al. The autophagic protein FYCO1 controls TNFRSF10/TRAIL receptor induced apoptosis and is inactivated by CASP8 (caspase 8). Autophagy. 2023;:1-19 pubmed publisher
Tedesco B, Vendredy L, Adriaenssens E, Cozzi M, Asselbergh B, Crippa V, et al. HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathies. Autophagy. 2023;19:2217-2239 pubmed publisher
Choi Y, Choi Y, Ko S, Cheon C, Kim T. Investigation of Molecular Mechanisms Involved in Sensitivity to the Anti-Cancer Activity of Costunolide in Breast Cancer Cells. Int J Mol Sci. 2023;24: pubmed publisher
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Tan J, Finkel T. A phosphoinositide signalling pathway mediates rapid lysosomal repair. Nature. 2022;609:815-821 pubmed publisher
Desideri E, Castelli S, Dorard C, Toifl S, Grazi G, Ciriolo M, et al. Impaired degradation of YAP1 and IL6ST by chaperone-mediated autophagy promotes proliferation and migration of normal and hepatocellular carcinoma cells. Autophagy. 2022;:1-11 pubmed publisher
Oh C, Dolatabadi N, Cieplak P, Diaz Meco M, Moscat J, Nolan J, et al. S-Nitrosylation of p62 Inhibits Autophagic Flux to Promote α-Synuclein Secretion and Spread in Parkinson's Disease and Lewy Body Dementia. J Neurosci. 2022;42:3011-3024 pubmed publisher
Lisowski C, Dias J, Costa S, Silva R, Mano M, Eulalio A. Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication. Autophagy. 2021;:1-16 pubmed publisher
Sulkshane P, Ram J, Thakur A, Reis N, Kleifeld O, Glickman M. Ubiquitination and receptor-mediated mitophagy converge to eliminate oxidation-damaged mitochondria during hypoxia. Redox Biol. 2021;45:102047 pubmed publisher
Du Y, Wang J, Xiong J, Fang N, Ji W. VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions. Mol Biol Cell. 2021;32:1474-1486 pubmed publisher
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Scotto Rosato A, Montefusco S, Soldati C, Di Paola S, Capuozzo A, Monfregola J, et al. TRPML1 links lysosomal calcium to autophagosome biogenesis through the activation of the CaMKKβ/VPS34 pathway. Nat Commun. 2019;10:5630 pubmed publisher
Guarascio R, Salih D, Yasvoina M, Edwards F, Cheetham M, van der Spuy J. Negative Regulator of Ubiquitin-Like Protein 1 modulates the autophagy-lysosomal pathway via p62 to facilitate the extracellular release of tau following proteasome impairment. Hum Mol Genet. 2020;29:80-96 pubmed publisher
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Kasitinon S, Eskiocak U, Martin M, Bezwada D, Khivansara V, Tasdogan A, et al. TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling. Cell Rep. 2019;28:2293-2305.e9 pubmed publisher
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Kasper L, König A, Koenig P, Gresnigt M, Westman J, Drummond R, et al. The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes. Nat Commun. 2018;9:4260 pubmed publisher
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Schulze R, Weller S, Schroeder B, Krueger E, Chi S, Casey C, et al. Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes. J Cell Biol. 2013;203:315-26 pubmed publisher
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product information
Catalog Number :
sc-20011
Product Name :
LAMP-1 (H4A3) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
H4A3
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
LAMP-1
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, IHC(P), FCM
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA