product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
Plk (F-8) Antibody
catalog :
sc-17783
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F-8
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 72
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:100; loading ...; fig 7e
van de Kooij B, Creixell P, van Vlimmeren A, Joughin B, Miller C, Haider N, et al. Comprehensive substrate specificity profiling of the human Nek kinome reveals unexpected signaling outputs. elife. 2019;8: pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Li W, Wang H, Zhao X, Duan H, Cheng B, Liu Y, et al. A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair. Sci Adv. 2019;5:eaau7566 pubmed publisher
  • western blot; human; loading ...; fig 1a
Zhang J, Bu X, Wang H, Zhu Y, Geng Y, Nihira N, et al. Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance. Nature. 2018;553:91-95 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig s6d
Takaki T, Montagner M, Serres M, Le Berre M, Russell M, Collinson L, et al. Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Nat Commun. 2017;8:16013 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 6e; S6i
Vallejo A, Perurena N, Guruceaga E, Mazur P, Martínez Canarias S, Zandueta C, et al. An integrative approach unveils FOSL1 as an oncogene vulnerability in KRAS-driven lung and pancreatic cancer. Nat Commun. 2017;8:14294 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s7r
Shumilov A, Tsai M, Schlosser Y, Kratz A, Bernhardt K, Fink S, et al. Epstein-Barr virus particles induce centrosome amplification and chromosomal instability. Nat Commun. 2017;8:14257 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2b
Ruf S, Heberle A, Langelaar Makkinje M, Gelino S, Wilkinson D, Gerbeth C, et al. PLK1 (polo like kinase 1) inhibits MTOR complex 1 and promotes autophagy. Autophagy. 2017;13:486-505 pubmed publisher
  • immunohistochemistry; human; 1:25; fig 1
Cholewa B, Ndiaye M, Huang W, Liu X, Ahmad N. Small molecule inhibition of polo-like kinase 1 by volasertib (BI 6727) causes significant melanoma growth delay and regression in vivo. Cancer Lett. 2017;385:179-187 pubmed publisher
  • western blot; human; loading ...; fig 1c
Liu J, Wan L, Liu J, Yuan Z, Zhang J, Guo J, et al. Cdh1 inhibits WWP2-mediated ubiquitination of PTEN to suppress tumorigenesis in an APC-independent manner. Cell Discov. 2016;2:15044 pubmed publisher
  • western blot; human; loading ...; fig 4h
Chung H, Park J, Lee N, Kim H, Jang C. Phosphorylation of Astrin Regulates Its Kinetochore Function. J Biol Chem. 2016;291:17579-92 pubmed publisher
  • western blot; human; fig 5
Wang Z, Shen L, Chang C, Zhang X, Chen Z, Li L, et al. Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity. Sci Rep. 2016;6:25385 pubmed publisher
  • immunocytochemistry; human; fig 3
Zhao W, Liu J, Zhang X, Deng L. MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1. J Cell Biol. 2016;212:829-43 pubmed publisher
  • western blot; human; 1:50; fig 3
Ercilla A, Llopis A, Feu S, Aranda S, Ernfors P, Freire R, et al. New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress. Nucleic Acids Res. 2016;44:4745-62 pubmed publisher
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 1
Lasek A, McPherson B, Trueman N, Burkard M. The Functional Significance of Posttranslational Modifications on Polo-Like Kinase 1 Revealed by Chemical Genetic Complementation. PLoS ONE. 2016;11:e0150225 pubmed publisher
  • western blot; human; fig 2
Chen N, Chyau C, Lee Y, Tseng H, Chou F. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells. Sci Rep. 2016;6:20417 pubmed publisher
  • western blot; mouse; loading ...; fig 9c
Naylor R, Jeganathan K, Cao X, van Deursen J. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016;126:543-59 pubmed publisher
  • immunocytochemistry; mouse; 1:300; fig 4
Marangos P, Stevense M, Niaka K, Lagoudaki M, Nabti I, Jessberger R, et al. DNA damage-induced metaphase I arrest is mediated by the spindle assembly checkpoint and maternal age. Nat Commun. 2015;6:8706 pubmed publisher
  • western blot; human; fig 4a
Nestal de Moraes G, Khongkow P, Gong C, Yao S, Gomes A, Ji Z, et al. Forkhead box K2 modulates epirubicin and paclitaxel sensitivity through FOXO3a in breast cancer. Oncogenesis. 2015;4:e167 pubmed publisher
  • western blot; human; 1:500; fig s13
Shukla A, Kong D, Sharma M, Magidson V, Loncarek J. Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation. Nat Commun. 2015;6:8077 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1
Tut T, Lim S, Dissanayake I, Descallar J, Chua W, Ng W, et al. Upregulated Polo-Like Kinase 1 Expression Correlates with Inferior Survival Outcomes in Rectal Cancer. PLoS ONE. 2015;10:e0129313 pubmed publisher
  • western blot; human; fig 1
Zhang Z, Chen L, Wang H, Ahmad N, Liu X. Inhibition of Plk1 represses androgen signaling pathway in castration-resistant prostate cancer. Cell Cycle. 2015;14:2142-8 pubmed publisher
  • western blot; human; fig 1
Kobayashi A, Hashizume C, Dowaki T, Wong R. Therapeutic potential of mitotic interaction between the nucleoporin Tpr and aurora kinase A. Cell Cycle. 2015;14:1447-58 pubmed publisher
  • western blot; human
Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  • western blot; human; fig 5
Kratz A, Bärenz F, Richter K, Hoffmann I. Plk4-dependent phosphorylation of STIL is required for centriole duplication. Biol Open. 2015;4:370-7 pubmed publisher
  • western blot; human; fig 5
Thomas Y, Peter M, Mechali F, Blanchard J, Coux O, Baldin V. Kizuna is a novel mitotic substrate for CDC25B phosphatase. Cell Cycle. 2014;13:3867-77 pubmed publisher
  • western blot; human
Bastos R, Cundell M, Barr F. KIF4A and PP2A-B56 form a spatially restricted feedback loop opposing Aurora B at the anaphase central spindle. J Cell Biol. 2014;207:683-93 pubmed publisher
  • western blot; human
Shao C, Ahmad N, Hodges K, Kuang S, Ratliff T, Liu X. Inhibition of polo-like kinase 1 (Plk1) enhances the antineoplastic activity of metformin in prostate cancer. J Biol Chem. 2015;290:2024-33 pubmed publisher
  • western blot; human
Wang D, Zhang P, Gao K, Tang Y, Jin X, Zhang Y, et al. PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation. PLoS ONE. 2014;9:e110296 pubmed publisher
  • western blot; human; 1:2000; fig 2
Chan Y, West S. Spatial control of the GEN1 Holliday junction resolvase ensures genome stability. Nat Commun. 2014;5:4844 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Van Der Meer R, Song H, Park S, Abdulkadir S, Roh M. RNAi screen identifies a synthetic lethal interaction between PIM1 overexpression and PLK1 inhibition. Clin Cancer Res. 2014;20:3211-21 pubmed publisher
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Matthess Y, Raab M, Knecht R, Becker S, Strebhardt K. Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Mol Oncol. 2014;8:596-608 pubmed publisher
  • western blot; human; fig 2
Laguette N, Brégnard C, Hue P, Basbous J, Yatim A, Larroque M, et al. Premature activation of the SLX4 complex by Vpr promotes G2/M arrest and escape from innate immune sensing. Cell. 2014;156:134-45 pubmed publisher
  • immunocytochemistry; human; 1:500
Gao M, Rendtlew Danielsen J, Wei L, Zhou D, Xu Q, Li M, et al. A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity. PLoS ONE. 2012;7:e49687 pubmed publisher
  • western blot; human
Gao D, Inuzuka H, Tseng A, Chin R, Toker A, Wei W. Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction. Nat Cell Biol. 2009;11:397-408 pubmed publisher
Lindel F, Dodt C, Weidner N, Noll M, Bergemann F, Behrendt R, et al. TraFo-CRISPR: Enhanced Genome Engineering by Transient Foamy Virus Vector-Mediated Delivery of CRISPR/Cas9 Components. Mol Ther Nucleic Acids. 2019;18:708-726 pubmed publisher
Gul N, Karlsson J, Tängemo C, Linsefors S, Tuyizere S, Perkins R, et al. The MTH1 inhibitor TH588 is a microtubule-modulating agent that eliminates cancer cells by activating the mitotic surveillance pathway. Sci Rep. 2019;9:14667 pubmed publisher
Jin Z, Suk N, Kim N. TP53BP1 regulates chromosome alignment and spindle bipolarity in mouse oocytes. Mol Reprod Dev. 2019;: pubmed publisher
Adriaans I, Basant A, Ponsioen B, Glotzer M, Lens S. PLK1 plays dual roles in centralspindlin regulation during cytokinesis. J Cell Biol. 2019;218:1250-1264 pubmed publisher
Wang H, Qiu Z, Liu B, Wu Y, Ren J, Liu Y, et al. PLK1 targets CtIP to promote microhomology-mediated end joining. Nucleic Acids Res. 2018;46:10724-10739 pubmed publisher
Jiang M, Rao R, Wang J, Wang J, Xu L, Wu L, et al. The TSC1-mTOR-PLK axis regulates the homeostatic switch from Schwann cell proliferation to myelination in a stage-specific manner. Glia. 2018;66:1947-1959 pubmed publisher
Liu K, Fang L, Sun H, Pan Z, Zhang J, Chen J, et al. Targeting Polo-like Kinase 1 by a Novel Pyrrole-Imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth In Vivo. Mol Cancer Ther. 2018;17:988-1002 pubmed publisher
Lv C, Zhao G, Sun X, Wang P, Xie N, Luo J, et al. Acetylation of FOXM1 is essential for its transactivation and tumor growth stimulation. Oncotarget. 2016;7:60366-60382 pubmed publisher
Müllers E, Silva Cascales H, Jaiswal H, Saurin A, Lindqvist A. Nuclear translocation of Cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase. Cell Cycle. 2014;13:2733-43 pubmed publisher
Kong D, Farmer V, Shukla A, James J, Gruskin R, Kiriyama S, et al. Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles. J Cell Biol. 2014;206:855-65 pubmed publisher
Charles J, Fuchs J, Hefter M, Vischedyk J, Kleint M, Vogiatzi F, et al. Monitoring the dynamics of clonal tumour evolution in vivo using secreted luciferases. Nat Commun. 2014;5:3981 pubmed publisher
Nunes Bastos R, Gandhi S, Baron R, Gruneberg U, Nigg E, Barr F. Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A. J Cell Biol. 2013;202:605-21 pubmed publisher
Choudhary A, Lera R, Martowicz M, Oxendine K, Laffin J, Weaver B, et al. Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis. Proc Natl Acad Sci U S A. 2013;110:13026-31 pubmed publisher
Hou X, Li Z, Huang W, Li J, Staiger C, Kuang S, et al. Plk1-dependent microtubule dynamics promotes androgen receptor signaling in prostate cancer. Prostate. 2013;73:1352-63 pubmed publisher
Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J. Efficient and specific gene knockdown by small interfering RNAs produced in bacteria. Nat Biotechnol. 2013;31:350-6 pubmed publisher
Baker D, Dawlaty M, Wijshake T, Jeganathan K, Malureanu L, van Ree J, et al. Increased expression of BubR1 protects against aneuploidy and cancer and extends healthy lifespan. Nat Cell Biol. 2013;15:96-102 pubmed publisher
Liu X, Song B, Tang J, Liu W, Kuang S, Liu X. Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment. Mol Cell Biol. 2012;32:4053-67 pubmed publisher
Hu C, Ozlu N, Coughlin M, Steen J, Mitchison T. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell. 2012;23:2702-11 pubmed publisher
Kachaner D, Filipe J, Laplantine E, Bauch A, Bennett K, Superti Furga G, et al. Plk1-dependent phosphorylation of optineurin provides a negative feedback mechanism for mitotic progression. Mol Cell. 2012;45:553-66 pubmed publisher
Hu C, Coughlin M, Mitchison T. Midbody assembly and its regulation during cytokinesis. Mol Biol Cell. 2012;23:1024-34 pubmed publisher
Lee K, Rhee K. PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis. J Cell Biol. 2011;195:1093-101 pubmed publisher
Song B, Liu X, Davis K, Liu X. Plk1 phosphorylation of Orc2 promotes DNA replication under conditions of stress. Mol Cell Biol. 2011;31:4844-56 pubmed publisher
Song B, Davis K, Liu X, Lee H, Smith M, Liu X. Inhibition of Polo-like kinase 1 reduces beta-amyloid-induced neuronal cell death in Alzheimer's disease. Aging (Albany NY). 2011;3:846-51 pubmed
Foley E, Maldonado M, Kapoor T. Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase. Nat Cell Biol. 2011;13:1265-71 pubmed publisher
Ke Y, Huh J, Warrington R, Wu N, Leng M, Zhang J, et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J. 2011;30:3309-21 pubmed publisher
Cheng N, Zhang W, Chen W, Jin J, Cui X, Butte N, et al. A mammalian monothiol glutaredoxin, Grx3, is critical for cell cycle progression during embryogenesis. FEBS J. 2011;278:2525-2539 pubmed publisher
Zeng K, Bastos R, Barr F, Gruneberg U. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2010;191:1315-32 pubmed publisher
Bastos R, Barr F. Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission. J Cell Biol. 2010;191:751-60 pubmed publisher
Studach L, Wang W, Weber G, Tang J, Hullinger R, Malbrue R, et al. Polo-like kinase 1 activated by the hepatitis B virus X protein attenuates both the DNA damage checkpoint and DNA repair resulting in partial polyploidy. J Biol Chem. 2010;285:30282-93 pubmed publisher
Liu X, Li H, Song B, Liu X. Polo-like kinase 1 phosphorylation of G2 and S-phase-expressed 1 protein is essential for p53 inactivation during G2 checkpoint recovery. EMBO Rep. 2010;11:626-32 pubmed publisher
Amato A, Schillaci T, Lentini L, Di Leonardo A. CENPA overexpression promotes genome instability in pRb-depleted human cells. Mol Cancer. 2009;8:119 pubmed publisher
Amato A, Lentini L, Schillaci T, Iovino F, Di Leonardo A. RNAi mediated acute depletion of retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation. BMC Cell Biol. 2009;10:79 pubmed publisher
Yang X, Li H, Deng A, Liu X. Plk1 phosphorylation of Topors is involved in its degradation. Mol Biol Rep. 2010;37:3023-8 pubmed publisher
Yang X, Li H, Liu X, Deng A, Liu X. Cdc2-mediated phosphorylation of CLIP-170 is essential for its inhibition of centrosome reduplication. J Biol Chem. 2009;284:28775-82 pubmed publisher
Steinhauser I, Langer K, Strebhardt K, Spänkuch B. Uptake of plasmid-loaded nanoparticles in breast cancer cells and effect on Plk1 expression. J Drug Target. 2009;17:627-37 pubmed publisher
Gao D, Inuzuka H, Korenjak M, Tseng A, Wu T, Wan L, et al. Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways. Mol Biol Cell. 2009;20:3305-16 pubmed publisher
Wu Z, Yang X, Weber G, Liu X. Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem. 2008;283:25503-13 pubmed publisher
Siddiqui H, Fox S, Gunawardena R, Knudsen E. Loss of RB compromises specific heterochromatin modifications and modulates HP1alpha dynamics. J Cell Physiol. 2007;211:131-7 pubmed
product information
Product Name :
Plk (F-8) Antibody
Gene Name :
PLK1
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-17783
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
Plk
reactive species  :
m, r, h
Application :
WB, IP, IF, ELISA
Epitope :
261-412 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA