product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
ERK 2 (D-2) Antibody
catalog :
sc-1647
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
D-2
reactivity :
human, mouse, rat, Xenopus laevis
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 83
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig s11a
An W, Lin H, Ma L, Zhang C, Zheng Y, Cheng Q, et al. Progesterone activates GPR126 to promote breast cancer development via the Gi pathway. Proc Natl Acad Sci U S A. 2022;119:e2117004119 pubmed publisher
  • western blot; mouse; loading ...; fig 2b
Myers D, Abram C, Wildes D, Belwafa A, Welsh A, Schulze C, et al. Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity. Front Immunol. 2020;11:576310 pubmed publisher
  • western blot; mouse; loading ...; fig 3g, s7c
Grohmann M, Wiede F, Dodd G, Gurzov E, Ooi G, Butt T, et al. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC. Cell. 2018;175:1289-1306.e20 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s7a
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens P, Neel B. Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2. Nat Commun. 2017;8:466 pubmed publisher
  • western blot; human; loading ...; fig 1a
Asensio Juan E, Fueyo R, PAPPA S, Iacobucci S, Badosa C, Lois S, et al. The histone demethylase PHF8 is a molecular safeguard of the IFNγ response. Nucleic Acids Res. 2017;45:3800-3811 pubmed publisher
  • western blot; human; loading ...; fig 1a
Takahashi M, Li Y, Dillon T, Stork P. Phosphorylation of Rap1 by cAMP-dependent Protein Kinase (PKA) Creates a Binding Site for KSR to Sustain ERK Activation by cAMP. J Biol Chem. 2017;292:1449-1461 pubmed publisher
  • western blot; human; loading ...; fig 1a
Li Y, Dillon T, Takahashi M, Earley K, Stork P. Protein Kinase A-independent Ras Protein Activation Cooperates with Rap1 Protein to Mediate Activation of the Extracellular Signal-regulated Kinases (ERK) by cAMP. J Biol Chem. 2016;291:21584-21595 pubmed
  • western blot; mouse; fig 1
Hey F, Giblett S, Forrest S, Herbert C, Pritchard C. Phosphorylations of Serines 21/9 in Glycogen Synthase Kinase 3α/β Are Not Required for Cell Lineage Commitment or WNT Signaling in the Normal Mouse Intestine. PLoS ONE. 2016;11:e0156877 pubmed publisher
  • western blot; human; loading ...; fig 5a
Blee A, Liu S, Wang L, Huang H. BET bromodomain-mediated interaction between ERG and BRD4 promotes prostate cancer cell invasion. Oncotarget. 2016;7:38319-38332 pubmed publisher
  • western blot; human; fig 4
Choi H, Kim M, Choi Y, Shin Y, Cho S, Ko S. Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation. BMC Complement Altern Med. 2016;16:122 pubmed publisher
  • western blot; human; fig 7
Pivonello C, Negri M, De Martino M, Napolitano M, De Angelis C, Provvisiero D, et al. The dual targeting of insulin and insulin-like growth factor 1 receptor enhances the mTOR inhibitor-mediated antitumor efficacy in hepatocellular carcinoma. Oncotarget. 2016;7:9718-31 pubmed publisher
  • western blot; mouse; 1:2000; fig s3
Drilon A, Somwar R, Wagner J, Vellore N, Eide C, Zabriskie M, et al. A Novel Crizotinib-Resistant Solvent-Front Mutation Responsive to Cabozantinib Therapy in a Patient with ROS1-Rearranged Lung Cancer. Clin Cancer Res. 2016;22:2351-8 pubmed publisher
  • immunohistochemistry; Xenopus laevis; 1:50; fig 5
  • immunocytochemistry; mouse; 1:200; fig 2
  • western blot; mouse; 1:1000; fig 2
Sipieter F, Cappe B, Gonzalez Pisfil M, Spriet C, Bodart J, Cailliau Maggio K, et al. Novel Reporter for Faithful Monitoring of ERK2 Dynamics in Living Cells and Model Organisms. PLoS ONE. 2015;10:e0140924 pubmed publisher
  • western blot; human; 1:2000
Davare M, Vellore N, Wagner J, Eide C, Goodman J, Drilon A, et al. Structural insight into selectivity and resistance profiles of ROS1 tyrosine kinase inhibitors. Proc Natl Acad Sci U S A. 2015;112:E5381-90 pubmed publisher
  • western blot; mouse; fig s4
Wightman S, Uppal A, Pitroda S, Ganai S, Burnette B, Stack M, et al. Oncogenic CXCL10 signalling drives metastasis development and poor clinical outcome. Br J Cancer. 2015;113:327-35 pubmed publisher
  • western blot; human; fig 1
Chan S, Selth L, Li Y, Nyquist M, Miao L, Bradner J, et al. Targeting chromatin binding regulation of constitutively active AR variants to overcome prostate cancer resistance to endocrine-based therapies. Nucleic Acids Res. 2015;43:5880-97 pubmed publisher
  • immunoprecipitation; human; fig 3
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 4
Warren D, Tajsic T, Porter L, Minaisah R, Cobb A, Jacob A, et al. Nesprin-2-dependent ERK1/2 compartmentalisation regulates the DNA damage response in vascular smooth muscle cell ageing. Cell Death Differ. 2015;22:1540-50 pubmed publisher
  • immunocytochemistry; human; 1:200
  • western blot; human
Gonzalez Granado J, Navarro Puche A, Molina Sánchez P, Blanco Berrocal M, Viana R, Font de Mora J, et al. Sorting nexin 6 enhances lamin a synthesis and incorporation into the nuclear envelope. PLoS ONE. 2014;9:e115571 pubmed publisher
  • western blot; human
Kugel C, Hartsough E, Davies M, Setiady Y, Aplin A. Function-blocking ERBB3 antibody inhibits the adaptive response to RAF inhibitor. Cancer Res. 2014;74:4122-32 pubmed publisher
  • western blot; human
Kardos G, Dai M, Robertson G. Growth inhibitory effects of large subunit ribosomal proteins in melanoma. Pigment Cell Melanoma Res. 2014;27:801-12 pubmed publisher
  • western blot; human
Li Y, Takahashi M, Stork P. Ras-mutant cancer cells display B-Raf binding to Ras that activates extracellular signal-regulated kinase and is inhibited by protein kinase A phosphorylation. J Biol Chem. 2013;288:27646-57 pubmed publisher
  • western blot; human; 1:500
Slowik A, Merres J, Elfgen A, Jansen S, Mohr F, Wruck C, et al. Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells. Mol Neurodegener. 2012;7:55 pubmed publisher
  • western blot; human; 1:500
Brandenburg L, Jansen S, Albrecht L, Merres J, Gerber J, Pufe T, et al. CpG oligodeoxynucleotides induce the expression of the antimicrobial peptide cathelicidin in glial cells. J Neuroimmunol. 2013;255:18-31 pubmed publisher
  • immunohistochemistry; mouse
Mizrak S, Renault Mihara F, Parraga M, Bogerd J, van de Kant H, Lopez Casas P, et al. Phosphoprotein enriched in astrocytes-15 is expressed in mouse testis and protects spermatocytes from apoptosis. Reproduction. 2007;133:743-51 pubmed
Wang W, Zhu Y, Sun Z, Jin C, Wang X. Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-β/SMAD2 signaling. Arthritis Res Ther. 2021;23:84 pubmed publisher
Schattauer S, Bedini A, Summers F, Reilly Treat A, Andrews M, Land B, et al. Reactive oxygen species (ROS) generation is stimulated by κ opioid receptor activation through phosphorylated c-Jun N-terminal kinase and inhibited by p38 mitogen-activated protein kinase (MAPK) activation. J Biol Chem. 2019;294:16884-16896 pubmed publisher
Ma L, Yan Y, Bai Y, Yang Y, Pan Y, Gang X, et al. Overcoming EZH2 Inhibitor Resistance by Taxane in PTEN-Mutated Cancer. Theranostics. 2019;9:5020-5034 pubmed publisher
Chiche J, Reverso Meinietti J, Mouchotte A, Rubio Patiño C, Mhaidly R, Villa E, et al. GAPDH Expression Predicts the Response to R-CHOP, the Tumor Metabolic Status, and the Response of DLBCL Patients to Metabolic Inhibitors. Cell Metab. 2019;29:1243-1257.e10 pubmed publisher
Torrino S, Roustan F, Kaminski L, Bertero T, Pisano S, Ambrosetti D, et al. UBTD1 is a mechano-regulator controlling cancer aggressiveness. EMBO Rep. 2019;20: pubmed publisher
Ohanna M, Cerezo M, Nottet N, Bille K, Didier R, Béranger G, et al. Pivotal role of NAMPT in the switch of melanoma cells toward an invasive and drug-resistant phenotype. Genes Dev. 2018;32:448-461 pubmed publisher
Rubio Patiño C, Bossowski J, De Donatis G, Mondragon L, Villa E, Aira L, et al. Low-Protein Diet Induces IRE1α-Dependent Anticancer Immunosurveillance. Cell Metab. 2018;27:828-842.e7 pubmed publisher
Yang Y, Blee A, Wang D, An J, Pan Y, Yan Y, et al. Loss of FOXO1 Cooperates with TMPRSS2-ERG Overexpression to Promote Prostate Tumorigenesis and Cell Invasion. Cancer Res. 2017;77:6524-6537 pubmed publisher
Niewolik D, Peter I, Butscher C, Schwarz K. Autoinhibition of the Nuclease ARTEMIS Is Mediated by a Physical Interaction between Its Catalytic and C-terminal Domains. J Biol Chem. 2017;292:3351-3365 pubmed publisher
Wang Z, Luo Y, Shao Q, Kinlock B, Wang C, Hildreth J, et al. Heat-stable molecule derived from Streptococcus cristatus induces APOBEC3 expression and inhibits HIV-1 replication. PLoS ONE. 2014;9:e106078 pubmed publisher
Subramani A, Alsidawi S, Jagannathan S, Sumita K, Sasaki A, Aronow B, et al. The brain microenvironment negatively regulates miRNA-768-3p to promote K-ras expression and lung cancer metastasis. Sci Rep. 2013;3:2392 pubmed publisher
Botton T, Yeh I, Nelson T, Vemula S, Sparatta A, Garrido M, et al. Recurrent BRAF kinase fusions in melanocytic tumors offer an opportunity for targeted therapy. Pigment Cell Melanoma Res. 2013;26:845-51 pubmed publisher
Ahmadiantehrani S, Ron D. Dopamine D2 receptor activation leads to an up-regulation of glial cell line-derived neurotrophic factor via G??-Erk1/2-dependent induction of Zif268. J Neurochem. 2013;125:193-204 pubmed publisher
Kaplan F, Kugel C, Dadpey N, Shao Y, Abel E, Aplin A. SHOC2 and CRAF mediate ERK1/2 reactivation in mutant NRAS-mediated resistance to RAF inhibitor. J Biol Chem. 2012;287:41797-807 pubmed publisher
Lv J, Zhao Z, Chen Y, Wang Q, Tao Y, Yang L, et al. The chinese herbal decoction danggui buxue tang inhibits angiogenesis in a rat model of liver fibrosis. Evid Based Complement Alternat Med. 2012;2012:284963 pubmed publisher
Jeseta M, Marin M, Tichovska H, Melicharova P, Cailliau Maggio K, Martoriati A, et al. Nitric oxide-donor SNAP induces Xenopus eggs activation. PLoS ONE. 2012;7:e41509 pubmed publisher
Takahashi N, Sakurai T, Bozdagi Gunal O, Dorr N, Moy J, Krug L, et al. Increased expression of receptor phosphotyrosine phosphatase-?/? is associated with molecular, cellular, behavioral and cognitive schizophrenia phenotypes. Transl Psychiatry. 2011;1:e8 pubmed publisher
Amatangelo M, Goodyear S, Varma D, Stearns M. c-Myc expression and MEK1-induced Erk2 nuclear localization are required for TGF-beta induced epithelial-mesenchymal transition and invasion in prostate cancer. Carcinogenesis. 2012;33:1965-75 pubmed publisher
Chan S, Li Y, Dehm S. Androgen receptor splice variants activate androgen receptor target genes and support aberrant prostate cancer cell growth independent of canonical androgen receptor nuclear localization signal. J Biol Chem. 2012;287:19736-49 pubmed publisher
Shao Y, Aplin A. ERK2 phosphorylation of serine 77 regulates Bmf pro-apoptotic activity. Cell Death Dis. 2012;3:e253 pubmed publisher
Baens M, Finalet Ferreiro J, Tousseyn T, Urbankova H, Michaux L, de Leval L, et al. t(X;14)(p11.4;q32.33) is recurrent in marginal zone lymphoma and up-regulates GPR34. Haematologica. 2012;97:184-8 pubmed publisher
Makrodouli E, Oikonomou E, Koc M, Andera L, Sasazuki T, Shirasawa S, et al. BRAF and RAS oncogenes regulate Rho GTPase pathways to mediate migration and invasion properties in human colon cancer cells: a comparative study. Mol Cancer. 2011;10:118 pubmed publisher
Menon M, Kotlyarov A, Gaestel M. SB202190-induced cell type-specific vacuole formation and defective autophagy do not depend on p38 MAP kinase inhibition. PLoS ONE. 2011;6:e23054 pubmed publisher
Ohanna M, Giuliano S, Bonet C, Imbert V, Hofman V, Zangari J, et al. Senescent cells develop a PARP-1 and nuclear factor-{kappa}B-associated secretome (PNAS). Genes Dev. 2011;25:1245-61 pubmed publisher
Liu B, Willette Brown J, Liu S, Chen X, Fischer S, Hu Y. IKK? represses a network of inflammation and proliferation pathways and elevates c-Myc antagonists and differentiation in a dose-dependent manner in the skin. Cell Death Differ. 2011;18:1854-64 pubmed publisher
Hedden L, Benes C, Soltoff S. P2X(7) receptor antagonists display agonist-like effects on cell signaling proteins. Biochim Biophys Acta. 2011;1810:532-42 pubmed publisher
Majumdar A, Ghosh A, Datta S, Prudner B, Datta B. p67/MetAP2 suppresses K-RasV12-mediated transformation of NIH3T3 mouse fibroblasts in culture and in athymic mice. Biochemistry. 2010;49:10146-57 pubmed publisher
Horrix C, Raviv Z, Flescher E, Voss C, Berger M. Plant ribosome-inactivating proteins type II induce the unfolded protein response in human cancer cells. Cell Mol Life Sci. 2011;68:1269-81 pubmed publisher
Soltoff S, Asara J, Hedden L. Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells. J Biol Chem. 2010;285:36330-8 pubmed publisher
Pinto A, Crespo P. Analysis of ERKs' dimerization by electrophoresis. Methods Mol Biol. 2010;661:335-42 pubmed publisher
Dong Q, Cheng Z, Chang W, Blackman B, Conte F, Hu J, et al. Naturally-occurring mutation in the calcium-sensing receptor reveals the significance of extracellular domain loop III region for class C G-protein-coupled receptor function. J Clin Endocrinol Metab. 2010;95:E245-52 pubmed publisher
Kim T, Franco H, Jung S, Qin J, Broaddus R, Lydon J, et al. The synergistic effect of Mig-6 and Pten ablation on endometrial cancer development and progression. Oncogene. 2010;29:3770-80 pubmed publisher
Xia X, Park E, Liu B, Willette Brown J, Gong W, Wang J, et al. Reduction of IKKalpha expression promotes chronic ultraviolet B exposure-induced skin inflammation and carcinogenesis. Am J Pathol. 2010;176:2500-8 pubmed publisher
Soltoff S, Hedden L. Isoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells. J Biol Chem. 2010;285:13337-48 pubmed publisher
Lee D, Li Y, Chang S, Zhou J, Ho H, Chiu J, et al. Oscillatory flow-induced proliferation of osteoblast-like cells is mediated by alphavbeta3 and beta1 integrins through synergistic interactions of focal adhesion kinase and Shc with phosphatidylinositol 3-kinase and the Akt/mTOR/p70S6K pathway. J Biol Chem. 2010;285:30-42 pubmed publisher
Warren D, Tajsic T, Mellad J, Searles R, Zhang Q, Shanahan C. Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation. J Biol Chem. 2010;285:1311-20 pubmed publisher
Yeh C, Chiu J, Lee C, Lee P, Shih Y, Sun J, et al. Estrogen augments shear stress-induced signaling and gene expression in osteoblast-like cells via estrogen receptor-mediated expression of beta1-integrin. J Bone Miner Res. 2010;25:627-39 pubmed publisher
Ronca R, Gualandi L, Crescini E, Calza S, Presta M, Dell Era P. Fibroblast growth factor receptor-1 phosphorylation requirement for cardiomyocyte differentiation in murine embryonic stem cells. J Cell Mol Med. 2009;13:1489-98 pubmed publisher
Turchi L, Fareh M, Aberdam E, Kitajima S, Simpson F, Wicking C, et al. ATF3 and p15PAF are novel gatekeepers of genomic integrity upon UV stress. Cell Death Differ. 2009;16:728-37 pubmed publisher
Greenberg J, Shields D, Barillas S, Acevedo L, Murphy E, Huang J, et al. A role for VEGF as a negative regulator of pericyte function and vessel maturation. Nature. 2008;456:809-13 pubmed publisher
Medawar A, Virolle T, Rostagno P, de la Forest Divonne S, Gambaro K, Rouleau M, et al. DeltaNp63 is essential for epidermal commitment of embryonic stem cells. PLoS ONE. 2008;3:e3441 pubmed publisher
Janes S, Ofstad T, Campbell D, Eddaoudi A, Warnes G, Davies D, et al. PI3-kinase-dependent activation of apoptotic machinery occurs on commitment of epidermal keratinocytes to terminal differentiation. Cell Res. 2009;19:328-39 pubmed publisher
Soltoff S, Hedden L. Regulation of ERK1/2 by ouabain and Na-K-ATPase-dependent energy utilization and AMPK activation in parotid acinar cells. Am J Physiol Cell Physiol. 2008;295:C590-9 pubmed publisher
Turchi L, Aberdam E, Mazure N, Pouyssegur J, Deckert M, Kitajima S, et al. Hif-2alpha mediates UV-induced apoptosis through a novel ATF3-dependent death pathway. Cell Death Differ. 2008;15:1472-80 pubmed publisher
Dufour C, Holy X, Marie P. Transforming growth factor-beta prevents osteoblast apoptosis induced by skeletal unloading via PI3K/Akt, Bcl-2, and phospho-Bad signaling. Am J Physiol Endocrinol Metab. 2008;294:E794-801 pubmed publisher
Lee D, Yeh C, Chang S, Lee P, Chien S, Cheng C, et al. Integrin-mediated expression of bone formation-related genes in osteoblast-like cells in response to fluid shear stress: roles of extracellular matrix, Shc, and mitogen-activated protein kinase. J Bone Miner Res. 2008;23:1140-9 pubmed publisher
Dufour C, Holy X, Marie P. Skeletal unloading induces osteoblast apoptosis and targets alpha5beta1-PI3K-Bcl-2 signaling in rat bone. Exp Cell Res. 2007;313:394-403 pubmed
Hahn C, Wang H, Cho D, Talbot K, Gur R, Berrettini W, et al. Altered neuregulin 1-erbB4 signaling contributes to NMDA receptor hypofunction in schizophrenia. Nat Med. 2006;12:824-8 pubmed
Ueda Y, Neel N, Schutyser E, Raman D, Richmond A. Deletion of the COOH-terminal domain of CXC chemokine receptor 4 leads to the down-regulation of cell-to-cell contact, enhanced motility and proliferation in breast carcinoma cells. Cancer Res. 2006;66:5665-75 pubmed
Lien S, Usami S, Chien S, Chiu J. Phosphatidylinositol 3-kinase/Akt pathway is involved in transforming growth factor-beta1-induced phenotypic modulation of 10T1/2 cells to smooth muscle cells. Cell Signal. 2006;18:1270-8 pubmed
Plourde D, Soltoff S. Ouabain potentiates the activation of ERK1/2 by carbachol in parotid gland epithelial cells; inhibition of ERK1/2 reduces Na(+)-K(+)-ATPase activity. Am J Physiol Cell Physiol. 2006;290:C702-10 pubmed
Roberts M, Drosopoulos K, Vasileiou I, Stricker M, Taoufik E, Maercker C, et al. Microarray analysis of the differential transformation mediated by Kirsten and Harvey Ras oncogenes in a human colorectal adenocarcinoma cell line. Int J Cancer. 2006;118:616-27 pubmed
Drosopoulos K, Roberts M, Cermak L, Sasazuki T, Shirasawa S, Andera L, et al. Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005;280:22856-67 pubmed
Laurent Matha V, Maruani Herrmann S, Prébois C, Beaujouin M, Glondu M, Noel A, et al. Catalytically inactive human cathepsin D triggers fibroblast invasive growth. J Cell Biol. 2005;168:489-99 pubmed
Calderwood S. Regulatory interfaces between the stress protein response and other gene expression programs in the cell. Methods. 2005;35:139-48 pubmed
Thyss R, Virolle V, Imbert V, Peyron J, Aberdam D, Virolle T. NF-kappaB/Egr-1/Gadd45 are sequentially activated upon UVB irradiation to mediate epidermal cell death. EMBO J. 2005;24:128-37 pubmed
Datta B, Datta R, Majumdar A, Ghosh A. The stability of eukaryotic initiation factor 2-associated glycoprotein, p67, increases during skeletal muscle differentiation and that inhibits the phosphorylation of extracellular signal-regulated kinases 1 and 2. Exp Cell Res. 2005;303:174-82 pubmed
Teramoto H, Castellone M, Malek R, Letwin N, Frank B, Gutkind J, et al. Autocrine activation of an osteopontin-CD44-Rac pathway enhances invasion and transformation by H-RasV12. Oncogene. 2005;24:489-501 pubmed
Janes S, Watt F. Switch from alphavbeta5 to alphavbeta6 integrin expression protects squamous cell carcinomas from anoikis. J Cell Biol. 2004;166:419-31 pubmed
product information
Product Name :
ERK 2 (D-2) Antibody
Gene Name :
MAPK1
Size :
200 µg/ml
Catalog Number :
sc-1647
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
ERK 2
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
C-terminal (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA