product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
IkappaB-alpha (H-4) Antibody
catalog :
sc-1643
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
H-4
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 67
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:500; loading ...; fig 3a
Wedgwood S, Gerard K, Halloran K, Hanhauser A, Monacelli S, Warford C, et al. Intestinal Dysbiosis and the Developing Lung: The Role of Toll-Like Receptor 4 in the Gut-Lung Axis. Front Immunol. 2020;11:357 pubmed publisher
  • western blot; human; loading ...; fig 4a
Zoi I, Karamouzis M, Xingi E, Sarantis P, Thomaidou D, Lembessis P, et al. Combining RANK/RANKL and ERBB-2 targeting as a novel strategy in ERBB-2-positive breast carcinomas. Breast Cancer Res. 2019;21:132 pubmed publisher
  • western blot; human; loading ...; fig 1a
Meshram S, Paul D, Manne R, Choppara S, Sankaran G, Agrawal Y, et al. FBXO32 activates NF-κB through IκBα degradation in inflammatory and genotoxic stress. Int J Biochem Cell Biol. 2017;92:134-140 pubmed publisher
  • western blot; human; loading ...; fig 5g
Amigo Jiménez I, Bailón E, Aguilera Montilla N, García Marco J, García Pardo A. Gene expression profile induced by arsenic trioxide in chronic lymphocytic leukemia cells reveals a central role for heme oxygenase-1 in apoptosis and regulation of matrix metalloproteinase-9. Oncotarget. 2016;7:83359-83377 pubmed publisher
  • western blot; mouse; fig 5
Yu Y, Cui Y, Zhao Y, Liu S, Song G, Jiao P, et al. The binding capability of plasma phospholipid transfer protein, but not HDL pool size, is critical to repress LPS induced inflammation. Sci Rep. 2016;6:20845 pubmed publisher
  • western blot; human; fig 3
Amigo Jiménez I, Bailón E, Aguilera Montilla N, Terol M, García Marco J, García Pardo A. Bone marrow stroma-induced resistance of chronic lymphocytic leukemia cells to arsenic trioxide involves Mcl-1 upregulation and is overcome by inhibiting the PI3Kδ or PKCβ signaling pathways. Oncotarget. 2015;6:44832-48 pubmed publisher
  • western blot; mouse
He L, Zang A, Du M, Ma D, Yuan C, Zhou C, et al. mTOR regulates TLR-induced c-fos and Th1 responses to HBV and HCV vaccines. Virol Sin. 2015;30:174-89 pubmed publisher
  • immunocytochemistry; human; fig 7
House C, Wang B, Ceniccola K, Williams R, Simaan M, Olender J, et al. Voltage-gated Na+ Channel Activity Increases Colon Cancer Transcriptional Activity and Invasion Via Persistent MAPK Signaling. Sci Rep. 2015;5:11541 pubmed publisher
  • western blot; rat; fig 2
Neira Peña T, Rojas Mancilla E, Munoz Vio V, Perez R, Gutierrez Hernandez M, Bustamante D, et al. Perinatal asphyxia leads to PARP-1 overactivity, p65 translocation, IL-1β and TNF-α overexpression, and apoptotic-like cell death in mesencephalon of neonatal rats: prevention by systemic neonatal nicotinamide administration. Neurotox Res. 2015;27:453-65 pubmed publisher
  • western blot; rat; 1:200
Ortiz F, Acuña Castroviejo D, Doerrier C, Dayoub J, López L, Venegas C, et al. Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation-induced oral mucositis. J Pineal Res. 2015;58:34-49 pubmed publisher
  • western blot; human; 1:100
Chondrogiannis G, Kastamoulas M, Kanavaros P, Vartholomatos G, Bai M, Baltogiannis D, et al. Cytokine effects on cell viability and death of prostate carcinoma cells. Biomed Res Int. 2014;2014:536049 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 8e
Clauzure M, Valdivieso A, Massip Copiz M, Schulman G, Teiber M, Santa Coloma T. Disruption of interleukin-1? autocrine signaling rescues complex I activity and improves ROS levels in immortalized epithelial cells with impaired cystic fibrosis transmembrane conductance regulator (CFTR) function. PLoS ONE. 2014;9:e99257 pubmed publisher
  • western blot; mouse
Hou J, Xia Y, Jiang R, Chen D, Xu J, Deng L, et al. PTPRO plays a dual role in hepatic ischemia reperfusion injury through feedback activation of NF-?B. J Hepatol. 2014;60:306-12 pubmed publisher
  • immunoprecipitation; human
  • western blot; human; 1:200
Fernandez G, Zaikos T, Khan S, Jacobi A, Behlke M, Zeichner S. Targeting I?B proteins for HIV latency activation: the role of individual I?B and NF-?B proteins. J Virol. 2013;87:3966-78 pubmed publisher
Barbero G, Castro M, Villanueva M, Quezada M, Fernández N, Demorrow S, et al. An Autocrine Wnt5a Loop Promotes NF-κB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma. Cells. 2019;8: pubmed publisher
Zhang Y, Dittmer D, Mieczkowski P, Host K, Fusco W, Duncan J, et al. RIG-I Detects Kaposi's Sarcoma-Associated Herpesvirus Transcripts in a RNA Polymerase III-Independent Manner. MBio. 2018;9: pubmed publisher
Shukla K, Sonowal H, Saxena A, Ramana K. Didymin prevents hyperglycemia-induced human umbilical endothelial cells dysfunction and death. Biochem Pharmacol. 2018;152:1-10 pubmed publisher
De Cicco P, Panza E, Armogida C, Ercolano G, Taglialatela Scafati O, Shokoohinia Y, et al. The Hydrogen Sulfide Releasing Molecule Acetyl Deacylasadisulfide Inhibits Metastatic Melanoma. Front Pharmacol. 2017;8:65 pubmed publisher
Deruy E, Nassour J, Martin N, Vercamer C, Malaquin N, Bertout J, et al. Level of macroautophagy drives senescent keratinocytes into cell death or neoplastic evasion. Cell Death Dis. 2014;5:e1577 pubmed publisher
Fasano E, Serini S, Mondella N, Trombino S, Celleno L, Lanza P, et al. Antioxidant and anti-inflammatory effects of selected natural compounds contained in a dietary supplement on two human immortalized keratinocyte lines. Biomed Res Int. 2014;2014:327452 pubmed publisher
Salazar N, Vallejos X, Siryk A, Rengo G, Cannavo A, Liccardo D, et al. GRK2 blockade with ?ARKct is essential for cardiac ?2-adrenergic receptor signaling towards increased contractility. Cell Commun Signal. 2013;11:64 pubmed publisher
Kastamoulas M, Chondrogiannis G, Kanavaros P, Vartholomatos G, Bai M, Briasoulis E, et al. Cytokine effects on cell survival and death of A549 lung carcinoma cells. Cytokine. 2013;61:816-25 pubmed publisher
Chiba T, Itoh T, Tabuchi M, Nakazawa T, Satou T. Interleukin-1? accelerates the onset of stroke in stroke-prone spontaneously hypertensive rats. Mediators Inflamm. 2012;2012:701976 pubmed publisher
Zhang N, Sun Q, Chen Z, Liu F, Jiang Y. Homeostatic regulatory role of Pokemon in NF-?B signaling: stimulating both p65 and I?B? expression in human hepatocellular carcinoma cells. Mol Cell Biochem. 2013;372:57-64 pubmed publisher
Huang W, Shang W, Wang H, Wu W, Hou S. Sirt1 overexpression protects murine osteoblasts against TNF-?-induced injury in vitro by suppressing the NF-?B signaling pathway. Acta Pharmacol Sin. 2012;33:668-74 pubmed publisher
Ndour P, Brocqueville G, Ouk T, Goormachtigh G, Morales O, Mougel A, et al. Inhibition of latent membrane protein 1 impairs the growth and tumorigenesis of latency II Epstein-Barr virus-transformed T cells. J Virol. 2012;86:3934-43 pubmed publisher
Li C, Chao L, Wang S, Chang H, Tsai M, Fang S, et al. Honokiol inhibits LPS-induced maturation and inflammatory response of human monocyte-derived dendritic cells. J Cell Physiol. 2011;226:2338-49 pubmed publisher
Kacimi R, Giffard R, Yenari M. Endotoxin-activated microglia injure brain derived endothelial cells via NF-?B, JAK-STAT and JNK stress kinase pathways. J Inflamm (Lond). 2011;8:7 pubmed publisher
Janda E, Visalli V, Colica C, Aprigliano S, Musolino V, Vadalà N, et al. The protective effect of tianeptine on Gp120-induced apoptosis in astroglial cells: role of GS and NOS, and NF-?B suppression. Br J Pharmacol. 2011;164:1590-9 pubmed publisher
Valovka T, Hottiger M. p65 controls NF-?B activity by regulating cellular localization of I?B?. Biochem J. 2011;434:253-63 pubmed publisher
Bhaskaran N, Shukla S, Srivastava J, Gupta S. Chamomile: an anti-inflammatory agent inhibits inducible nitric oxide synthase expression by blocking RelA/p65 activity. Int J Mol Med. 2010;26:935-40 pubmed
Marcora E, Kennedy M. The Huntington's disease mutation impairs Huntingtin's role in the transport of NF-?B from the synapse to the nucleus. Hum Mol Genet. 2010;19:4373-84 pubmed publisher
Zhu F, Dollé M, Berton T, Kuiper R, Capps C, Espejo A, et al. Mouse models for the p53 R72P polymorphism mimic human phenotypes. Cancer Res. 2010;70:5851-9 pubmed publisher
Andújar I, Recio M, Bacelli T, Giner R, Rios J. Shikonin reduces oedema induced by phorbol ester by interfering with IkappaBalpha degradation thus inhibiting translocation of NF-kappaB to the nucleus. Br J Pharmacol. 2010;160:376-88 pubmed publisher
Umenai T, Hirai H, Shime N, Nakaya T, Asahara T, Nomoto K, et al. Eradication of the commensal intestinal microflora by oral antimicrobials interferes with the host response to lipopolysaccharide. Eur J Clin Microbiol Infect Dis. 2010;29:633-41 pubmed publisher
Cohen Saidon C, Cohen A, Sigal A, Liron Y, Alon U. Dynamics and variability of ERK2 response to EGF in individual living cells. Mol Cell. 2009;36:885-93 pubmed publisher
Haddad O, Chotard Ghodsnia R, Verdier C, Duperray A. Tumor cell/endothelial cell tight contact upregulates endothelial adhesion molecule expression mediated by NFkappaB: differential role of the shear stress. Exp Cell Res. 2010;316:615-26 pubmed publisher
Cetindere T, Nambiar S, Santourlidis S, Essmann F, Hassan M. Induction of indoleamine 2, 3-dioxygenase by death receptor activation contributes to apoptosis of melanoma cells via mitochondrial damage-dependent ROS accumulation. Cell Signal. 2010;22:197-211 pubmed publisher
Bhushan S, Malik F, Kumar A, Isher H, Kaur I, Taneja S, et al. Activation of p53/p21/PUMA alliance and disruption of PI-3/Akt in multimodal targeting of apoptotic signaling cascades in cervical cancer cells by a pentacyclic triterpenediol from Boswellia serrata. Mol Carcinog. 2009;48:1093-108 pubmed publisher
Petrof E, Claud E, Sun J, Abramova T, Guo Y, Waypa T, et al. Bacteria-free solution derived from Lactobacillus plantarum inhibits multiple NF-kappaB pathways and inhibits proteasome function. Inflamm Bowel Dis. 2009;15:1537-47 pubmed publisher
Benitez D, Hermoso M, Pozo Guisado E, Fernandez Salguero P, Castellon E. Regulation of cell survival by resveratrol involves inhibition of NF kappa B-regulated gene expression in prostate cancer cells. Prostate. 2009;69:1045-54 pubmed publisher
Nunes E, Kuczera D, Brito G, Bonatto S, Yamazaki R, Tanhoffer R, et al. Beta-hydroxy-beta-methylbutyrate supplementation reduces tumor growth and tumor cell proliferation ex vivo and prevents cachexia in Walker 256 tumor-bearing rats by modifying nuclear factor-kappaB expression. Nutr Res. 2008;28:487-93 pubmed publisher
Wright C, Zhuang T, La P, Yang G, Dennery P. Hyperoxia-induced NF-kappaB activation occurs via a maturationally sensitive atypical pathway. Am J Physiol Lung Cell Mol Physiol. 2009;296:L296-306 pubmed publisher
Jin X, Qiu L, Zhang D, Zhang M, Wang Z, Guo Z, et al. Chemosensitization in non-small cell lung cancer cells by IKK inhibitor occurs via NF-kappaB and mitochondrial cytochrome c cascade. J Cell Mol Med. 2009;13:4596-607 pubmed publisher
Webster C, Kelly S, Koike M, Chock V, Giffard R, Yenari M. Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia. Neurobiol Dis. 2009;33:301-12 pubmed publisher
Pogson M, Holcombe M, Smallwood R, Qwarnstrom E. Introducing spatial information into predictive NF-kappaB modelling--an agent-based approach. PLoS ONE. 2008;3:e2367 pubmed publisher
Jin X, Wang Z, Qiu L, Zhang D, Guo Z, Gao Z, et al. Potential biomarkers involving IKK/RelA signal in early stage non-small cell lung cancer. Cancer Sci. 2008;99:582-9 pubmed publisher
Cai S, Brandt C. Induction of interleukin-6 in human retinal epithelial cells by an attenuated Herpes simplex virus vector requires viral replication and NFkappaB activation. Exp Eye Res. 2008;86:178-88 pubmed
Kim H, Yoon D, Lee W, Han S, Shrestha B, Kim C, et al. Phellinus linteus inhibits inflammatory mediators by suppressing redox-based NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophage. J Ethnopharmacol. 2007;114:307-15 pubmed
Feng G, Liu S, Wang G, Liu G. Lidocaine attenuates lipopolysaccharide-induced acute lung injury through inhibiting NF-kappaB activation. Pharmacology. 2008;81:32-40 pubmed
Merling R, Chen C, Hong S, Zhang L, Liu M, Kuo Y, et al. HTLV-1 Tax mutants that do not induce G1 arrest are disabled in activating the anaphase promoting complex. Retrovirology. 2007;4:35 pubmed
Zheng Z, Kim J, Ma H, Lee J, Yenari M. Anti-inflammatory effects of the 70 kDa heat shock protein in experimental stroke. J Cereb Blood Flow Metab. 2008;28:53-63 pubmed
Kim H, Yoon D, Kim C, Shrestha B, Chang W, Lim S, et al. Ethanol extract of Inonotus obliquus inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophage cells. J Med Food. 2007;10:80-9 pubmed
Romanatto T, Cesquini M, Amaral M, Roman E, Moraes J, Torsoni M, et al. TNF-alpha acts in the hypothalamus inhibiting food intake and increasing the respiratory quotient--effects on leptin and insulin signaling pathways. Peptides. 2007;28:1050-8 pubmed
Chiu J, Chen L, Lee C, Lee P, Lee D, Tsai M, et al. Mechanisms of induction of endothelial cell E-selectin expression by smooth muscle cells and its inhibition by shear stress. Blood. 2007;110:519-28 pubmed
Fernández Majada V, Aguilera C, Villanueva A, Vilardell F, Robert Moreno A, Aytes A, et al. Nuclear IKK activity leads to dysregulated notch-dependent gene expression in colorectal cancer. Proc Natl Acad Sci U S A. 2007;104:276-81 pubmed
Aguilera C, Fernández Majada V, Inglés Esteve J, Rodilla V, Bigas A, Espinosa L. Efficient nuclear export of p65-IkappaBalpha complexes requires 14-3-3 proteins. J Cell Sci. 2006;119:3695-704 pubmed
Hanashiro K, Sunagawa M, Tokeshi Y, Nakasone T, Ohta S, Nakamura M, et al. Antiallergic drugs, azelastine hydrochloride and epinastine hydrochloride, inhibit ongoing IgE secretion of rat IgE-producing hybridoma FE-3 cells. Eur J Pharmacol. 2006;547:174-83 pubmed
Wissel H, Burkhardt W, Rupp J, Wauer R, Rudiger M. Perfluorocarbons decrease Chlamydophila pneumoniae-mediated inflammatory responses of rat type II pneumocytes in vitro. Pediatr Res. 2006;60:264-9 pubmed
Redondo Muñoz J, Escobar Díaz E, Samaniego R, Terol M, Garcia Marco J, Garcia Pardo A. MMP-9 in B-cell chronic lymphocytic leukemia is up-regulated by alpha4beta1 integrin or CXCR4 engagement via distinct signaling pathways, localizes to podosomes, and is involved in cell invasion and migration. Blood. 2006;108:3143-51 pubmed
Goormachtigh G, Ouk T, Mougel A, Tranchand Bunel D, Masy E, Le Clorennec C, et al. Autoactivation of the Epstein-Barr virus oncogenic protein LMP1 during type II latency through opposite roles of the NF-kappaB and JNK signaling pathways. J Virol. 2006;80:7382-93 pubmed
Ogino T, Hosako M, Hiramatsu K, Omori M, Ozaki M, Okada S. Oxidative modification of IkappaB by monochloramine inhibits tumor necrosis factor alpha-induced NF-kappaB activation. Biochim Biophys Acta. 2005;1746:135-42 pubmed
Mun H, Aosai F, Norose K, Piao L, Fang H, Akira S, et al. Toll-like receptor 4 mediates tolerance in macrophages stimulated with Toxoplasma gondii-derived heat shock protein 70. Infect Immun. 2005;73:4634-42 pubmed
Conde R, Xavier J, McLoughlin C, Chinkers M, Ovsenek N. Protein phosphatase 5 is a negative modulator of heat shock factor 1. J Biol Chem. 2005;280:28989-96 pubmed
Yang J, Chen J, Luo L, Lin S, Li D, Hu S. Tetramethylpyrazine protected photoreceptor cells of rats by modulating nuclear translocation of NF-kappaB. Acta Pharmacol Sin. 2005;26:887-92 pubmed
Dieckhoff K, Graf P, Beinhauer B, Schwaerzler C, Carballido J, Neumann C, et al. Deficient translocation of c-Rel is associated with impaired Th1 cytokine production in T cells from atopic dermatitis patients. Exp Dermatol. 2005;14:17-25 pubmed
Löscher M, Fortschegger K, Ritter G, Wostry M, Voglauer R, Schmid J, et al. Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome. Biochem J. 2005;388:593-603 pubmed
product information
Product Name :
IkappaB-alpha (H-4) Antibody
Gene Name :
NFKBIA
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-1643
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
IkappaB-alpha
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), FCM, ELISA
Epitope :
1-317 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA