product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
HIF-1alpha (28b) Antibody
catalog :
sc-13515
quantity :
200 µg/ml
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
28b
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 38
Published Application/Species/Sample/DilutionReference
  • chromatin immunoprecipitation; human; loading ...; fig 10e
Du J, Yu Q, Liu Y, Du S, Huang L, Xu D, et al. A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis. Theranostics. 2021;11:4207-4231 pubmed publisher
  • western blot; human; 1:300; loading ...; fig 6a
Potilinski M, Ortiz G, Salica J, Lopez E, Fernández Acquier M, Chuluyan E, et al. Elucidating the mechanism of action of alpha-1-antitrypsin using retinal pigment epithelium cells exposed to high glucose. Potential use in diabetic retinopathy. PLoS ONE. 2020;15:e0228895 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 4e
Tang H, Feng H, Wang M, Zhu Q, Liu Y, Jiang Y. In vivo longitudinal and multimodal imaging of hypoxia-inducible factor 1α and angiogenesis in breast cancer. Chin Med J (Engl). 2020;133:205-211 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5a
  • western blot; mouse; loading ...; fig 4g
Jassim A, Inman D. Evidence of Hypoxic Glial Cells in a Model of Ocular Hypertension. Invest Ophthalmol Vis Sci. 2019;60:1-15 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; fig 2a
Badowska Kozakiewicz A, Sobol M, Patera J. Expression of multidrug resistance protein P-glycoprotein in correlation with markers of hypoxia (HIF-1?, EPO, EPO-R) in invasive breast cancer with metastasis to lymph nodes. Arch Med Sci. 2017;13:1303-1314 pubmed publisher
  • western blot; human; loading ...; fig 2a
Li S, Liu H, Tang S, Li X, Wang X. MicroRNA-150 regulates glycolysis by targeting von Hippel-Lindau in glioma cells. Am J Transl Res. 2017;9:1058-1066 pubmed
  • western blot; human; 1:500; loading ...; fig 3A
Guo Q, He J, Shen F, Zhang W, Yang X, Zhang C, et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett. 2017;13:949-954 pubmed publisher
  • immunohistochemistry; human; fig 1e
Oh E, Kim J, Kim J, Kim S, Lee J, Hong S, et al. NQO1 inhibits proteasome-mediated degradation of HIF-1α. Nat Commun. 2016;7:13593 pubmed publisher
  • immunoprecipitation; human; 1:200; loading ...; fig 2f
  • western blot; human; 1:1000; loading ...; fig 2f
Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher
  • western blot; human; 1:500; fig 1
Shen G, Ning N, Zhao X, Liu X, Wang G, Wang T, et al. Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. Mol Med Rep. 2015;12:8055-61 pubmed publisher
  • immunocytochemistry; human; fig 4
Rodríguez C, Reidel S, Bal de Kier Joffé E, Jasnis M, Fiszman G. Autophagy Protects from Trastuzumab-Induced Cytotoxicity in HER2 Overexpressing Breast Tumor Spheroids. PLoS ONE. 2015;10:e0137920 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; fig 1
  • western blot; human; 1:1000; fig 1
Li X, Yang X, Biskup E, Zhou J, Li H, Wu Y, et al. Co-expression of CXCL8 and HIF-1α is associated with metastasis and poor prognosis in hepatocellular carcinoma. Oncotarget. 2015;6:22880-9 pubmed
  • immunohistochemistry - paraffin section; human; fig 4
  • western blot; human; loading ...; fig 3
Zhang Y, Fan N, Yang J. Expression and clinical significance of hypoxia-inducible factor 1?, Snail and E-cadherin in human ovarian cancer cell lines. Mol Med Rep. 2015;12:3393-3399 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 4
  • western blot; human; fig 4
Basu S, Majumder S, Bhowal A, Ghosh A, Naskar S, Nandy S, et al. A study of molecular signals deregulating mismatch repair genes in prostate cancer compared to benign prostatic hyperplasia. PLoS ONE. 2015;10:e0125560 pubmed publisher
  • EMSA; human; 1 ug
Schaffner C, Mwinyi J, Gai Z, Thasler W, Eloranta J, Kullak Ublick G. The organic solute transporters alpha and beta are induced by hypoxia in human hepatocytes. Liver Int. 2015;35:1152-61 pubmed publisher
Qiu H, Chen F, Chen M. MicroRNA-138 negatively regulates the hypoxia-inducible factor 1α to suppress melanoma growth and metastasis. Biol Open. 2019;8: pubmed publisher
You Z, Yu M, Zhang Y, Shi K. Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll‑like receptor‑4‑NADPH oxidase 4 signaling pathway. Mol Med Rep. 2019;19:382-390 pubmed publisher
Ji W, Wang L, He S, Yan L, Li T, Wang J, et al. Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle. PLoS ONE. 2018;13:e0208474 pubmed publisher
Kondratskyi A, Kondratska K, Vanden Abeele F, Gordienko D, Dubois C, Toillon R, et al. Ferroquine, the next generation antimalarial drug, has antitumor activity. Sci Rep. 2017;7:15896 pubmed publisher
Zhang Y, Wang T, Wei J, He J, Wang T, Liu Y, et al. What's the remedy for the distal necrosis of DIEP flap, better venous drain or more arterial supply?. PLoS ONE. 2017;12:e0171651 pubmed publisher
Xu W, Zhou W, Cheng M, Wang J, Liu Z, He S, et al. Hypoxia activates Wnt/β-catenin signaling by regulating the expression of BCL9 in human hepatocellular carcinoma. Sci Rep. 2017;7:40446 pubmed publisher
Li S, Shao J, Zhou Y, Friis T, Yao J, Shi B, et al. The impact of Wnt signalling and hypoxia on osteogenic and cementogenic differentiation in human periodontal ligament cells. Mol Med Rep. 2016;14:4975-4982 pubmed publisher
Gao C, Li S, Zhao T, Chen J, Ren H, Zhang H, et al. SCF, regulated by HIF-1α, promotes pancreatic ductal adenocarcinoma cell progression. PLoS ONE. 2015;10:e0121338 pubmed publisher
Wang B, Ding Y, Fan P, Wang B, Xu J, Wang W. Expression and significance of MMP2 and HIF-1? in hepatocellular carcinoma. Oncol Lett. 2014;8:539-546 pubmed
Talaei F, Van Praag V, Shishavan M, Landheer S, Buikema H, Henning R. Increased protein aggregation in Zucker diabetic fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide. BMC Cell Biol. 2014;15:1 pubmed publisher
Li F, Lu Y, Ding M, Napierala D, Abbassi S, Chen Y, et al. Runx2 contributes to murine Col10a1 gene regulation through direct interaction with its cis-enhancer. J Bone Miner Res. 2011;26:2899-910 pubmed publisher
Heeg S, Hirt N, Queisser A, Schmieg H, Thaler M, Kunert H, et al. EGFR overexpression induces activation of telomerase via PI3K/AKT-mediated phosphorylation and transcriptional regulation through Hif1-alpha in a cellular model of oral-esophageal carcinogenesis. Cancer Sci. 2011;102:351-60 pubmed publisher
Wang W, Reiser Erkan C, Michalski C, Raggi M, Quan L, Yupei Z, et al. Hypoxia inducible BHLHB2 is a novel and independent prognostic marker in pancreatic ductal adenocarcinoma. Biochem Biophys Res Commun. 2010;401:422-8 pubmed publisher
Yu R, Ng P, Tan T, Chu D, Wu R, Kong R. Enhancement of hypoxia-induced gene expression in fish liver by the aryl hydrocarbon receptor (AhR) ligand, benzo[a]pyrene (BaP). Aquat Toxicol. 2008;90:235-42 pubmed publisher
Okami N, Kawamata T, Yamamoto G, Okada Y, Hori T, Tachikawa T. Laser microdissection-based analysis of hypoxia- and thioredoxin-related genes in human stable carotid plaques. Cardiovasc Pathol. 2009;18:294-300 pubmed publisher
Pisano C, Zuco V, De Cesare M, Benedetti V, Vesci L, Foderà R, et al. Intracellular accumulation and DNA damage persistence as determinants of human squamous cell carcinoma hypersensitivity to the novel camptothecin ST1968. Eur J Cancer. 2008;44:1332-40 pubmed publisher
Tal R, Shaish A, Bangio L, Peled M, Breitbart E, Harats D. Activation of C-transactivation domain is essential for optimal HIF-1 alpha-mediated transcriptional and angiogenic effects. Microvasc Res. 2008;76:1-6 pubmed publisher
Han Z, Ren H, Zhao H, Chi Y, Chen K, Zhou B, et al. Hypoxia-inducible factor (HIF)-1 alpha directly enhances the transcriptional activity of stem cell factor (SCF) in response to hypoxia and epidermal growth factor (EGF). Carcinogenesis. 2008;29:1853-61 pubmed publisher
Shi L, Huang J, Han B. Hypoxia inducible factor-1alpha mediates protective effects of ischemic preconditioning on ECV-304 endothelial cells. World J Gastroenterol. 2007;13:2369-73 pubmed
Ioachim E, Michael M, Salmas M, Michael M, Stavropoulos N, Malamou Mitsi V. Hypoxia-inducible factors HIF-1alpha and HIF-2alpha expression in bladder cancer and their associations with other angiogenesis-related proteins. Urol Int. 2006;77:255-63 pubmed
Yoo Y, Kong G, Lee M. Metastasis-associated protein 1 enhances stability of hypoxia-inducible factor-1alpha protein by recruiting histone deacetylase 1. EMBO J. 2006;25:1231-41 pubmed
Anderson C, Hoare S, Ashcroft M, Bilsland A, Keith W. Hypoxic regulation of telomerase gene expression by transcriptional and post-transcriptional mechanisms. Oncogene. 2006;25:61-9 pubmed
Kyzas P, Stefanou D, Batistatou A, Agnantis N. Hypoxia-induced tumor angiogenic pathway in head and neck cancer: an in vivo study. Cancer Lett. 2005;225:297-304 pubmed
product information
Product Name :
HIF-1alpha (28b) Antibody
Gene Name :
HIF1A
Size :
200 µg/ml
Catalog Number :
sc-13515
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
HIF-1alpha
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P)
Epitope :
329-530 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA