| Published Application/Species/Sample/Dilution | Reference |
|---|
- western blot; human; 1:1000; loading ...; fig s3a
| Allan L, Skowyra A, Rogers K, Zeller D, Clarke P. Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest. EMBO J. 2018;37: pubmed publisher |
- western blot; human; loading ...; fig 1a
| Zhang J, Bu X, Wang H, Zhu Y, Geng Y, Nihira N, et al. Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance. Nature. 2018;553:91-95 pubmed publisher |
- immunoprecipitation; human; loading ...; fig 1e
- western blot; human; loading ...; fig 1e
| Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher |
- proximity ligation assay; human; 1:250; loading ...; fig s1d
| Li J, Dang N, Wood D, Huang J. The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells. Sci Rep. 2017;7:41072 pubmed publisher |
- immunoprecipitation; human; loading ...; fig 1d
- western blot; human; loading ...; fig 1b
| Salsi V, Fantini S, Zappavigna V. NUP98 fusion oncoproteins interact with the APC/C(Cdc20) as a pseudosubstrate and prevent mitotic checkpoint complex binding. Cell Cycle. 2016;15:2275-87 pubmed publisher |
- ELISA; human; 1:1000; fig 1c
- western blot; human; 1:1000
| Sedgwick G, Larsen M, Lischetti T, Streicher W, Jersie Christensen R, Olsen J, et al. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling. MAbs. 2016;8:689-97 pubmed publisher |
- western blot; human; 1:1000; fig 1
| Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher |
- immunoprecipitation; human; fig s4b
| Craney A, Kelly A, Jia L, Fedrigo I, Yu H, Rape M. Control of APC/C-dependent ubiquitin chain elongation by reversible phosphorylation. Proc Natl Acad Sci U S A. 2016;113:1540-5 pubmed publisher |
- other; human; loading ...; fig st1
| Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher |
- western blot; human; fig s1
| Kaisari S, Sitry Shevah D, Miniowitz Shemtov S, Hershko A. Intermediates in the assembly of mitotic checkpoint complexes and their role in the regulation of the anaphase-promoting complex. Proc Natl Acad Sci U S A. 2016;113:966-71 pubmed publisher |
- immunoprecipitation; human
- western blot; human
| Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher |
- immunoprecipitation; human; 1:500
| Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher |
- immunocytochemistry; human; fig 2
- western blot; human; 1:500; fig 3
| Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher |
- immunocytochemistry; human; fig 6a
| Han J, Vitre B, Fachinetti D, Cleveland D. Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling. Proc Natl Acad Sci U S A. 2014;111:E4185-93 pubmed publisher |
- immunohistochemistry; human; 1:500
- western blot; human
| Hu K, Liao D, Wu W, Han A, Shi H, Wang F, et al. Targeting the anaphase-promoting complex/cyclosome (APC/C)- bromodomain containing 7 (BRD7) pathway for human osteosarcoma. Oncotarget. 2014;5:3088-100 pubmed |
- immunohistochemistry; mouse; 1:100
- western blot; mouse
| Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher |
- western blot; human; 1:1000
| Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher |
| Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher |
| Chu Z, Zhang X, Li Q, Hu G, Lian C, Geng S. CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway. Int J Oncol. 2019;54:1534-1544 pubmed publisher |
| Wild T, Budzowska M, Hellmuth S, Eibes S, Karemore G, Barisic M, et al. Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint. Cell Rep. 2018;25:2317-2328.e5 pubmed publisher |
| Rashid M, Mazur T, Ji W, Liu S, Taylor W. Analysis of the role of GSK3 in the mitotic checkpoint. Sci Rep. 2018;8:14259 pubmed publisher |
| Skowyra A, Allan L, Saurin A, Clarke P. USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability. Cell Rep. 2018;23:852-865 pubmed publisher |
| Kaisari S, Sitry Shevah D, Miniowitz Shemtov S, Teichner A, Hershko A. Role of CCT chaperonin in the disassembly of mitotic checkpoint complexes. Proc Natl Acad Sci U S A. 2017;114:956-961 pubmed publisher |
| Zhang G, Méndez B, Sedgwick G, Nilsson J. Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. Nat Commun. 2016;7:12256 pubmed publisher |
| Eytan E, Sitry Shevah D, Teichner A, Hershko A. Roles of different pools of the mitotic checkpoint complex and the mechanisms of their disassembly. Proc Natl Acad Sci U S A. 2013;110:10568-73 pubmed publisher |
| Sedgwick G, Hayward D, Di Fiore B, Pardo M, Yu L, Pines J, et al. Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex. EMBO J. 2013;32:303-14 pubmed publisher |
| Uzunova K, Dye B, Schutz H, Ladurner R, Petzold G, Toyoda Y, et al. APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex. Nat Struct Mol Biol. 2012;19:1116-23 pubmed publisher |
| Izawa D, Pines J. Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregation. J Cell Biol. 2012;199:27-37 pubmed publisher |
| Lai F, Hu K, Wu Y, Tang J, Sang Y, Cao J, et al. Human KIAA1018/FAN1 nuclease is a new mitotic substrate of APC/C(Cdh1). Chin J Cancer. 2012;31:440-8 pubmed publisher |
| Lian G, Lu J, Hu J, Zhang J, Cross S, Ferland R, et al. Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation. J Neurosci. 2012;32:7672-84 pubmed publisher |
| Kato T, Daigo Y, Aragaki M, Ishikawa K, Sato M, Kaji M. Overexpression of CDC20 predicts poor prognosis in primary non-small cell lung cancer patients. J Surg Oncol. 2012;106:423-30 pubmed publisher |
| Tipton A, Tipton M, Yen T, Liu S. Closed MAD2 (C-MAD2) is selectively incorporated into the mitotic checkpoint complex (MCC). Cell Cycle. 2011;10:3740-50 pubmed publisher |
| Mansfeld J, Collin P, Collins M, Choudhary J, Pines J. APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Nat Cell Biol. 2011;13:1234-43 pubmed publisher |
| Tipton A, Wang K, Link L, Bellizzi J, Huang H, Yen T, et al. BUBR1 and closed MAD2 (C-MAD2) interact directly to assemble a functional mitotic checkpoint complex. J Biol Chem. 2011;286:21173-9 pubmed publisher |
| Miniowitz Shemtov S, Teichner A, Sitry Shevah D, Hershko A. ATP is required for the release of the anaphase-promoting complex/cyclosome from inhibition by the mitotic checkpoint. Proc Natl Acad Sci U S A. 2010;107:5351-6 pubmed publisher |
| Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa Saito M, et al. Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1. Mol Biol Cell. 2008;19:1007-21 pubmed |
| Braunstein I, Miniowitz S, Moshe Y, Hershko A. Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint. Proc Natl Acad Sci U S A. 2007;104:4870-5 pubmed |
| Eytan E, Moshe Y, Braunstein I, Hershko A. Roles of the anaphase-promoting complex/cyclosome and of its activator Cdc20 in functional substrate binding. Proc Natl Acad Sci U S A. 2006;103:2081-6 pubmed |
