product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
UBF (F-9) Antibody
catalog :
sc-13125
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F-9
reactivity :
Lagranja shrew, human, mouse, rat, chicken
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunocytochemistry knockout validation
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry knockout validation; chicken; 1:250; fig 6
Nielsen C, Huttner D, Bizard A, Hirano S, Li T, Palmai Pallag T, et al. PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nat Commun. 2015;6:8962 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3g
  • western blot; human; loading ...; fig 3c
Lafita Navarro M, Kim M, Borenstein Auerbach N, Venkateswaran N, Hao Y, Ray R, et al. The aryl hydrocarbon receptor regulates nucleolar activity and protein synthesis in MYC-expressing cells. Genes Dev. 2018;32:1303-1308 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 1a
Chen J, Lobb I, Morin P, Novo S, Simpson J, Kennerknecht K, et al. Identification of a novel TIF-IA-NF-κB nucleolar stress response pathway. Nucleic Acids Res. 2018;46:6188-6205 pubmed publisher
  • western blot; human; loading ...; fig 3a
Edvardson S, Nicolae C, Agrawal P, Mignot C, Payne K, Prasad A, et al. Heterozygous De Novo UBTF Gain-of-Function Variant Is Associated with Neurodegeneration in Childhood. Am J Hum Genet. 2017;101:267-273 pubmed publisher
  • immunocytochemistry; human; 1:150; loading ...; fig 3b
Lee K, Zhang P, Kim H, Mitrea D, Sarkar M, Freibaum B, et al. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell. 2016;167:774-788.e17 pubmed publisher
  • immunocytochemistry; human; 1:250; fig 4
Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
  • immunocytochemistry; human; 1:500; fig s4
Feringa F, Krenning L, Koch A, van den Berg J, van den Broek B, Jalink K, et al. Hypersensitivity to DNA damage in antephase as a safeguard for genome stability. Nat Commun. 2016;7:12618 pubmed publisher
  • western blot; rat; loading ...; fig 5b
Ogasawara R, Fujita S, Hornberger T, Kitaoka Y, Makanae Y, Nakazato K, et al. The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise. Sci Rep. 2016;6:31142 pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig s2b
  • western blot; human; 1:500; loading ...; fig 3d
Dass R, Sarshad A, Carson B, Feenstra J, Kaur A, Obrdlik A, et al. Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I. PLoS Genet. 2016;12:e1006217 pubmed publisher
  • western blot; human; fig s2b
Chen S, Blank M, Iyer A, Huang B, Wang L, Grummt I, et al. SIRT7-dependent deacetylation of the U3-55k protein controls pre-rRNA processing. Nat Commun. 2016;7:10734 pubmed publisher
  • western blot; human; fig 5A
Yu F, Shen X, Fan L, Yu Z. Analysis of histone modifications at human ribosomal DNA in liver cancer cell. Sci Rep. 2015;5:18100 pubmed publisher
  • immunocytochemistry; human; fig 7a
Wandrey F, Montellese C, Koos K, Badertscher L, Bammert L, Cook A, et al. The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology. Mol Cell Biol. 2015;35:3491-503 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 2e
Voit R, Seiler J, Grummt I. Cooperative Action of Cdk1/cyclin B and SIRT1 Is Required for Mitotic Repression of rRNA Synthesis. PLoS Genet. 2015;11:e1005246 pubmed publisher
  • western blot; mouse; fig 2
Ide S, Déjardin J. End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter. Nat Commun. 2015;6:6674 pubmed publisher
  • immunocytochemistry; human
Ehm P, Nalaskowski M, Wundenberg T, Jücker M. The tumor suppressor SHIP1 colocalizes in nucleolar cavities with p53 and components of PML nuclear bodies. Nucleus. 2015;6:154-64 pubmed publisher
  • immunocytochemistry; human; fig 1
Schertzer M, Jouravleva K, Perderiset M, Dingli F, Loew D, Le Guen T, et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Res. 2015;43:1834-47 pubmed publisher
  • immunocytochemistry; human
Khalouei S, Chow A, Brown I. Localization of heat shock protein HSPA6 (HSP70B') to sites of transcription in cultured differentiated human neuronal cells following thermal stress. J Neurochem. 2014;131:743-54 pubmed publisher
  • immunocytochemistry; human; 1:300; fig s5
Larsen D, Hari F, Clapperton J, Gwerder M, Gutsche K, Altmeyer M, et al. The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Nat Cell Biol. 2014;16:792-803 pubmed publisher
  • immunocytochemistry; human; fig 2
Smith C, Matheson T, Trombly D, Sun X, Campeau E, Han X, et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014;25:2866-81 pubmed publisher
  • immunocytochemistry; Lagranja shrew
Zhdanova N, Draskovic I, Minina J, Karamysheva T, Novo C, Liu W, et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells. Mol Cell Biol. 2014;34:2786-99 pubmed publisher
  • immunocytochemistry; human; 1:50
  • western blot; human; 1:1000
Miyazawa N, Yoshikawa H, Magae S, Ishikawa H, Izumikawa K, Terukina G, et al. Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA. Genes Cells. 2014;19:273-86 pubmed publisher
  • immunocytochemistry; human
Wolf A, Mantri M, Heim A, Müller U, Fichter E, Mackeen M, et al. The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization. Biochem J. 2013;453:357-70 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Wang J, Leung J, Gong Z, Feng L, Shi X, Chen J. PHF6 regulates cell cycle progression by suppressing ribosomal RNA synthesis. J Biol Chem. 2013;288:3174-83 pubmed publisher
Potapova T, Unruh J, Yu Z, Rancati G, Li H, Stampfer M, et al. Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes. J Cell Biol. 2019;: pubmed publisher
Donnio L, Lagarou A, Sueur G, Mari P, Giglia Mari G. CSB-Dependent Cyclin-Dependent Kinase 9 Degradation and RNA Polymerase II Phosphorylation during Transcription-Coupled Repair. Mol Cell Biol. 2019;39: pubmed publisher
Hayashi Y, Fujimura A, Kato K, Udagawa R, Hirota T, Kimura K. Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Sci Adv. 2018;4:eaap7777 pubmed publisher
Pefani D, Tognoli M, Pirincci Ercan D, Gorgoulis V, O Neill E. MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B. EMBO J. 2018;37: pubmed publisher
You J, Dooley M, Kim C, Kim E, Xu W, Goodman C, et al. A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling. Sci Signal. 2018;11: pubmed publisher
Dilworth D, Upadhyay S, Bonnafous P, Edoo A, Bourbigot S, Pesek Jardim F, et al. The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module. Nucleic Acids Res. 2017;45:11989-12004 pubmed publisher
Gonyo P, Bergom C, Brandt A, Tsaih S, Sun Y, Bigley T, et al. SmgGDS is a transient nucleolar protein that protects cells from nucleolar stress and promotes the cell cycle by regulating DREAM complex gene expression. Oncogene. 2017;36:6873-6883 pubmed publisher
Xie Q, Li C, Song X, Wu L, Jiang Q, Qiu Z, et al. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription. Nucleic Acids Res. 2017;45:2472-2489 pubmed publisher
Ponti D, Bellenchi G, Puca R, Bastianelli D, Maroder M, Ragona G, et al. The transcription factor EGR1 localizes to the nucleolus and is linked to suppression of ribosomal precursor synthesis. PLoS ONE. 2014;9:e96037 pubmed publisher
Sobol M, Yildirim S, Philimonenko V, Marášek P, Castano E, Hozak P. UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity. Nucleus. 2013;4:478-86 pubmed publisher
Cong R, Das S, Douet J, Wong J, Buschbeck M, Mongelard F, et al. macroH2A1 histone variant represses rDNA transcription. Nucleic Acids Res. 2014;42:181-92 pubmed publisher
Cong R, Das S, Ugrinova I, Kumar S, Mongelard F, Wong J, et al. Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription. Nucleic Acids Res. 2012;40:9441-54 pubmed publisher
Rosa Garrido M, Ceballos L, Alonso Lecue P, Abraira C, Delgado M, Gandarillas A. A cell cycle role for the epigenetic factor CTCF-L/BORIS. PLoS ONE. 2012;7:e39371 pubmed publisher
Dieriks B, Van Oostveldt P. Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. Int J Mol Med. 2012;29:1031-8 pubmed publisher
van de Nobelen S, Rosa Garrido M, Leers J, Heath H, Soochit W, Joosen L, et al. CTCF regulates the local epigenetic state of ribosomal DNA repeats. Epigenetics Chromatin. 2010;3:19 pubmed publisher
Castle C, Cassimere E, Lee J, Denicourt C. Las1L is a nucleolar protein required for cell proliferation and ribosome biogenesis. Mol Cell Biol. 2010;30:4404-14 pubmed publisher
Sharov A, Masui S, Sharova L, Piao Y, Aiba K, Matoba R, et al. Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data. BMC Genomics. 2008;9:269 pubmed publisher
Frescas D, Guardavaccaro D, Bassermann F, Koyama Nasu R, Pagano M. JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes. Nature. 2007;450:309-13 pubmed
Berciano M, Novell M, Villagra N, Casafont I, Bengoechea R, Val Bernal J, et al. Cajal body number and nucleolar size correlate with the cell body mass in human sensory ganglia neurons. J Struct Biol. 2007;158:410-20 pubmed
Kopp K, Gasiorowski J, Chen D, Gilmore R, Norton J, Wang C, et al. Pol I transcription and pre-rRNA processing are coordinated in a transcription-dependent manner in mammalian cells. Mol Biol Cell. 2007;18:394-403 pubmed
product information
Product Name :
UBF (F-9) Antibody
Gene Name :
UBTF
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-13125
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
UBF
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), ELISA
Epitope :
1-220 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA