product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
PCAF (E-8) Antibody
catalog :
sc-13124
quantity :
200 µg/ml
price :
279 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E-8
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 34
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig s8e
  • western blot; human; loading ...; fig 5b
Perearnau A, Orlando S, Islam A, Gallastegui E, Martinez J, Jordan A, et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res. 2017;45:5086-5099 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 3a
Fournier M, Orpinell M, Grauffel C, Scheer E, Garnier J, Ye T, et al. KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification. Nat Commun. 2016;7:13227 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 5i
  • western blot; human; 1:200; loading ...; fig 1b, 4c
Bharathy N, Suriyamurthy S, Rao V, Ow J, Lim H, Chakraborty P, et al. P/CAF mediates PAX3-FOXO1-dependent oncogenesis in alveolar rhabdomyosarcoma. J Pathol. 2016;240:269-281 pubmed publisher
  • chromatin immunoprecipitation; human; fig 6
  • western blot; human; fig 3
Lu R, Hu X, Zhou J, Sun J, Zhu A, Xu X, et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ER?-positive breast cancer by degradation of NCoR. Nat Commun. 2016;7:12044 pubmed publisher
  • western blot; mouse; 1:1000
Tyler C, Hafez A, Solomon E, Allan A. Developmental exposure to 50 parts-per-billion arsenic influences histone modifications and associated epigenetic machinery in a region- and sex-specific manner in the adult mouse brain. Toxicol Appl Pharmacol. 2015;288:40-51 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 6
Almuzzaini B, Sarshad A, Farrants A, Percipalle P. Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation. BMC Biol. 2015;13:35 pubmed publisher
  • western blot; human
Zhang Z, Shi L, Song L, Ephrem E, Petri M, Sullivan K. Interferon regulatory factor 1 marks activated genes and can induce target gene expression in systemic lupus erythematosus. Arthritis Rheumatol. 2015;67:785-96 pubmed publisher
  • immunoprecipitation; human; fig s1
  • western blot; human; 1:500; fig s1
Crider A, Thakkar R, Ahmed A, Pillai A. Dysregulation of estrogen receptor beta (ERβ), aromatase (CYP19A1), and ER co-activators in the middle frontal gyrus of autism spectrum disorder subjects. Mol Autism. 2014;5:46 pubmed publisher
  • western blot; mouse; fig 6a
Herrera F, Yamaguchi T, Roelink H, Tjian R. Core promoter factor TAF9B regulates neuronal gene expression. elife. 2014;3:e02559 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; human
Sarshad A, Corcoran M, Al Muzzaini B, Borgonovo Brandter L, von Euler A, Lamont D, et al. Glycogen synthase kinase (GSK) 3? phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells. PLoS Genet. 2014;10:e1004390 pubmed publisher
  • chromatin immunoprecipitation; human; 3 ug/time
Gatta R, Mantovani R. Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters. Cell Cycle. 2010;9:2149-59 pubmed
Mi W, Zhang Y, Lyu J, Wang X, Tong Q, Peng D, et al. The ZZ-type zinc finger of ZZZ3 modulates the ATAC complex-mediated histone acetylation and gene activation. Nat Commun. 2018;9:3759 pubmed publisher
Qu T, Chen F, Wang J, Zhang Y, Cheng M, Sun W, et al. PCAF-mediated acetylation of Lin28B increases let-7 biogenesis in lung adenocarcinoma H1299 cells. BMC Cancer. 2018;18:27 pubmed publisher
Mohibi S, Srivastava S, Bele A, Mirza S, Band H, Band V. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol. 2016;36:2487-502 pubmed publisher
El Omari K, Hoosdally S, Tuladhar K, Karia D, Hall Ponsele E, Platonova O, et al. Structural basis for LMO2-driven recruitment of the SCL:E47bHLH heterodimer to hematopoietic-specific transcriptional targets. Cell Rep. 2013;4:135-47 pubmed publisher
Dangi Garimella S, Sahai V, Ebine K, Kumar K, Munshi H. Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression. PLoS ONE. 2013;8:e64566 pubmed publisher
Shen M, Zhou T, Xie W, Ling T, Zhu Q, Zong L, et al. The chromatin remodeling factor CSB recruits histone acetyltransferase PCAF to rRNA gene promoters in active state for transcription initiation. PLoS ONE. 2013;8:e62668 pubmed publisher
Sarshad A, Sadeghifar F, Louvet E, Mori R, Böhm S, Al Muzzaini B, et al. Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression. PLoS Genet. 2013;9:e1003397 pubmed publisher
Quinlan E, Culleton S, Wu S, Chiang C, Androphy E. Acetylation of conserved lysines in bovine papillomavirus E2 by p300. J Virol. 2013;87:1497-507 pubmed publisher
Gong A, Eischeid A, Xiao J, Zhao J, Chen D, Wang Z, et al. miR-17-5p targets the p300/CBP-associated factor and modulates androgen receptor transcriptional activity in cultured prostate cancer cells. BMC Cancer. 2012;12:492 pubmed publisher
Schlottmann S, Erkizan H, Barber Rotenberg J, Knights C, Cheema A, Uren A, et al. Acetylation Increases EWS-FLI1 DNA Binding and Transcriptional Activity. Front Oncol. 2012;2:107 pubmed publisher
Zhao J, Zhang L, Zhang Y, Shen Y. The differential regulation of Gap43 gene in the neuronal differentiation of P19 cells. J Cell Physiol. 2012;227:2645-53 pubmed publisher
Shiota M, Yokomizo A, Tada Y, Uchiumi T, Inokuchi J, Tatsugami K, et al. P300/CBP-associated factor regulates Y-box binding protein-1 expression and promotes cancer cell growth, cancer invasion and drug resistance. Cancer Sci. 2010;101:1797-806 pubmed publisher
Katsuno M, Adachi H, Minamiyama M, Waza M, Doi H, Kondo N, et al. Disrupted transforming growth factor-beta signaling in spinal and bulbar muscular atrophy. J Neurosci. 2010;30:5702-12 pubmed publisher
Leclerc G, Mou C, LeClerc G, Mian A, Barredo J. Histone deacetylase inhibitors induce FPGS mRNA expression and intracellular accumulation of long-chain methotrexate polyglutamates in childhood acute lymphoblastic leukemia: implications for combination therapy. Leukemia. 2010;24:552-62 pubmed publisher
Masatsugu T, Yamamoto K. Multiple lysine methylation of PCAF by Set9 methyltransferase. Biochem Biophys Res Commun. 2009;381:22-6 pubmed publisher
Mao X, Gluck N, Li D, Maine G, Li H, Zaidi I, et al. GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Genes Dev. 2009;23:849-61 pubmed publisher
Wu M, Zhang Y, Wu N, Shen Y. Histone marks and chromatin remodelers on the regulation of neurogenin1 gene in RA induced neuronal differentiation of P19 cells. J Cell Biochem. 2009;107:264-71 pubmed publisher
Kutluay S, DeVos S, Klomp J, Triezenberg S. Transcriptional coactivators are not required for herpes simplex virus type 1 immediate-early gene expression in vitro. J Virol. 2009;83:3436-49 pubmed publisher
Lee I, Finkel T. Regulation of autophagy by the p300 acetyltransferase. J Biol Chem. 2009;284:6322-8 pubmed publisher
Zhao L, Subramanian T, Chinnadurai G. Changes in C-terminal binding protein 2 (CtBP2) corepressor complex induced by E1A and modulation of E1A transcriptional activity by CtBP2. J Biol Chem. 2006;281:36613-23 pubmed
An B, Selva D, Hammond G, Rivero Muller A, Rahman N, Leung P. Steroid receptor coactivator-3 is required for progesterone receptor trans-activation of target genes in response to gonadotropin-releasing hormone treatment of pituitary cells. J Biol Chem. 2006;281:20817-24 pubmed
Tu Z, Prajapati S, Park K, Kelly N, Yamamoto Y, Gaynor R. IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression. J Biol Chem. 2006;281:6699-706 pubmed
Huang W, Chen C. Akt phosphorylation of p300 at Ser-1834 is essential for its histone acetyltransferase and transcriptional activity. Mol Cell Biol. 2005;25:6592-602 pubmed
product information
Product Name :
PCAF (E-8) Antibody
Gene Name :
KAT2B
Size :
200 µg/ml
Price :
279 USD
Catalog Number :
sc-13124
Type :
Monoclonal
Host :
mouse monoclonal
Antigen :
PCAF
reactive species  :
m, r, h
Application :
WB, IP, IF, IHC(P), ELISA
Epitope :
464-832 (h)
Delivery Time :
Next day delivery in US for orders placed by 3:00 PM; Two day delivery for orders shipped internationally from our US office; Next day delivery to European customers placing orders from our European office
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA