product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
AIF (E-1) Antibody
catalog :
sc-13116
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E-1
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, other
citations: 52
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 4, 5, 7
  • western blot; human; 1:250; loading ...; fig 2
Letkovska K, Babal P, Cierna Z, Schmidtova S, Lišková V, Kalavska K, et al. Prognostic Value of Apoptosis-Inducing Factor (AIF) in Germ Cell Tumors. Cancers (Basel). 2021;13: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 4b
Signes A, Cerutti R, Dickson A, Benincá C, Hinchy E, Ghezzi D, et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med. 2019;11: pubmed publisher
  • western blot; human; fig 1b
Sugiura A, Mattie S, Prudent J, McBride H. Newly born peroxisomes are a hybrid of mitochondrial and ER-derived pre-peroxisomes. Nature. 2017;542:251-254 pubmed publisher
  • western blot; human; 1:700; loading ...; fig 4a
Lu H, Yang X, Tian X, Tang S, Li L, Zhao S, et al. The in vitro and vivo anti-tumor effects and molecular mechanisms of suberoylanilide hydroxamic acid (SAHA) and MG132 on the aggressive phenotypes of gastric cancer cells. Oncotarget. 2016;7:56508-56525 pubmed publisher
  • western blot; human; fig 2
Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  • western blot; human; fig s8
Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • western blot; mouse; fig 6
  • western blot; rat; fig 2
Sabirzhanov B, Stoica B, Zhao Z, Loane D, Wu J, Dorsey S, et al. miR-711 upregulation induces neuronal cell death after traumatic brain injury. Cell Death Differ. 2016;23:654-68 pubmed publisher
  • western blot; human; 1:1000; fig 5
Loureiro R, Magalhães Novais S, Mesquita K, Baldeiras I, Sousa I, Tavares L, et al. Melatonin antiproliferative effects require active mitochondrial function in embryonal carcinoma cells. Oncotarget. 2015;6:17081-96 pubmed
  • immunocytochemistry; human; tbl 1
  • western blot; human; 1:1000; tbl 1
Vega Naredo I, Cunha Oliveira T, Serafim T, Sardao V, Oliveira P. Analysis of pro-apoptotic protein trafficking to and from mitochondria. Methods Mol Biol. 2015;1241:163-80 pubmed publisher
  • western blot; human; fig 4
Iwaniuk A, Jabłońska E, Jabłoński J, Ratajczak Wrona W, Garley M. Expression of selected proteins of the extrinsic and intrinsic pathways of apoptosis in human leukocytes exposed to N-nitrosodimethylamine. Hum Exp Toxicol. 2015;34:591-600 pubmed publisher
  • western blot; human
  • western blot; rat
  • western blot; mouse
Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C, et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014;34:10055-71 pubmed publisher
Yang M, Wang C, Zhou M, Bao L, Wang Y, Kumar A, et al. KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response. Nucleic Acids Res. 2022;50:6313-6331 pubmed publisher
Yang L, Li Y, Bhattacharya A, Zhang Y. Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants. Commun Biol. 2021;4:1373 pubmed publisher
Ivanova I, Perkins N. Hypoxia induces rapid, STAT3 and ROS dependent, mitochondrial translocation of RelA(p65) and IκBα. Biosci Rep. 2019;39: pubmed publisher
Ding L, Li J, Li W, Fang Z, Li N, Wu S, et al. p53- and ROS-mediated AIF pathway involved in TGEV-induced apoptosis. J Vet Med Sci. 2018;80:1775-1781 pubmed publisher
Scott A, Walker S, Krank J, Wilkinson A, Johnson K, Lewis E, et al. AIF promotes a JNK1-mediated cadherin switch independently of respiratory chain stabilization. J Biol Chem. 2018;293:14707-14722 pubmed publisher
Wang R, Li C, Qiao P, Xue Y, Zheng X, Chen H, et al. OGG1-initiated base excision repair exacerbates oxidative stress-induced parthanatos. Cell Death Dis. 2018;9:628 pubmed publisher
Grassilli E, Ianzano L, Bonomo S, Missaglia C, Cerrito M, Giovannoni R, et al. GSK3A is redundant with GSK3B in modulating drug resistance and chemotherapy-induced necroptosis. PLoS ONE. 2014;9:e100947 pubmed publisher
Teng F, Tang B. Nogo/RTN4 isoforms and RTN3 expression protect SH-SY5Y cells against multiple death insults. Mol Cell Biochem. 2013;384:7-19 pubmed publisher
Sosna J, Voigt S, Mathieu S, Lange A, Thon L, Davarnia P, et al. TNF-induced necroptosis and PARP-1-mediated necrosis represent distinct routes to programmed necrotic cell death. Cell Mol Life Sci. 2014;71:331-48 pubmed publisher
Lu J, Yu J, Lim S, Son Y, Kim D, Lee S, et al. Cellular mechanisms of the cytotoxic effects of the zearalenone metabolites ?-zearalenol and ?-zearalenol on RAW264.7 macrophages. Toxicol In Vitro. 2013;27:1007-17 pubmed publisher
Lewis E, Wilkinson A, Jackson J, Mehra R, Varambally S, Chinnaiyan A, et al. The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. J Biol Chem. 2012;287:43862-75 pubmed publisher
Sabirzhanov B, Stoica B, Hanscom M, Piao C, Faden A. Over-expression of HSP70 attenuates caspase-dependent and caspase-independent pathways and inhibits neuronal apoptosis. J Neurochem. 2012;123:542-54 pubmed publisher
Namkung J, Song J, Jo H, Kim M, Lew Y, Donahoe P, et al. Mullerian inhibiting substance induces apoptosis of human endometrial stromal cells in endometriosis. J Clin Endocrinol Metab. 2012;97:3224-30 pubmed publisher
Cotrim C, Fabris V, Doria M, Lindberg K, Gustafsson J, Amado F, et al. Estrogen receptor beta growth-inhibitory effects are repressed through activation of MAPK and PI3K signalling in mammary epithelial and breast cancer cells. Oncogene. 2013;32:2390-402 pubmed publisher
Favreau D, Meessen Pinard M, Desforges M, Talbot P. Human coronavirus-induced neuronal programmed cell death is cyclophilin d dependent and potentially caspase dispensable. J Virol. 2012;86:81-93 pubmed publisher
An H, Shin H, Jo S, Kim Y, Lee J, Paik S, et al. The survival effect of mitochondrial Higd-1a is associated with suppression of cytochrome C release and prevention of caspase activation. Biochim Biophys Acta. 2011;1813:2088-98 pubmed publisher
Yu J, Zheng Z, Son Y, Shi X, Jang Y, Lee J. Mycotoxin zearalenone induces AIF- and ROS-mediated cell death through p53- and MAPK-dependent signaling pathways in RAW264.7 macrophages. Toxicol In Vitro. 2011;25:1654-63 pubmed publisher
Ryan M, Jackson J, Hao Y, Leonard S, Alway S. Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice. Free Radic Biol Med. 2011;51:38-52 pubmed publisher
Ballot C, Kluza J, Lancel S, Martoriati A, Hassoun S, Mortier L, et al. Inhibition of mitochondrial respiration mediates apoptosis induced by the anti-tumoral alkaloid lamellarin D. Apoptosis. 2010;15:769-81 pubmed publisher
Son Y, Heo J, Kim T, Jeon Y, Kim J, Lee J. Over-expression of JunB inhibits mitochondrial stress and cytotoxicity in human lymphoma cells exposed to chronic oxidative stress. BMB Rep. 2010;43:57-61 pubmed
Levin V, Panchabhai S, Shen L, Kornblau S, Qiu Y, Baggerly K. Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines. J Proteome Res. 2010;9:179-91 pubmed publisher
Park K, Lim J, Kim H. Inhibitory mechanism of omega-3 fatty acids in pancreatic inflammation and apoptosis. Ann N Y Acad Sci. 2009;1171:421-7 pubmed publisher
Liu L, Yang C, Herzog C, Seth R, Kaushal G. Proteasome inhibitors prevent cisplatin-induced mitochondrial release of apoptosis-inducing factor and markedly ameliorate cisplatin nephrotoxicity. Biochem Pharmacol. 2010;79:137-46 pubmed publisher
Son Y, Jang Y, Heo J, Chung W, Choi K, Lee J. Apoptosis-inducing factor plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Apoptosis. 2009;14:796-808 pubmed publisher
Joshi A, Bondada V, Geddes J. Mitochondrial micro-calpain is not involved in the processing of apoptosis-inducing factor. Exp Neurol. 2009;218:221-7 pubmed publisher
Pontes H, Sousa C, Silva R, Fernandes E, Carmo H, Remião F, et al. Synergistic toxicity of ethanol and MDMA towards primary cultured rat hepatocytes. Toxicology. 2008;254:42-50 pubmed publisher
Wang Y, Luo J, Chen X, Chen H, Cramer S, Sun D. Gene inactivation of Na+/H+ exchanger isoform 1 attenuates apoptosis and mitochondrial damage following transient focal cerebral ischemia. Eur J Neurosci. 2008;28:51-61 pubmed publisher
Liu Y, Templeton D. Initiation of caspase-independent death in mouse mesangial cells by Cd2+: involvement of p38 kinase and CaMK-II. J Cell Physiol. 2008;217:307-18 pubmed publisher
Castera L, Hatzfeld Charbonnier A, Ballot C, Charbonnel F, Dhuiege E, Velu T, et al. Apoptosis-related mitochondrial dysfunction defines human monocyte-derived dendritic cells with impaired immuno-stimulatory capacities. J Cell Mol Med. 2009;13:1321-35 pubmed publisher
Havasi A, Li Z, Wang Z, Martin J, Botla V, Ruchalski K, et al. Hsp27 inhibits Bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem. 2008;283:12305-13 pubmed publisher
Gallego M, Ballot C, Kluza J, Hajji N, Martoriati A, Castera L, et al. Overcoming chemoresistance of non-small cell lung carcinoma through restoration of an AIF-dependent apoptotic pathway. Oncogene. 2008;27:1981-92 pubmed
Vaishnav R, Getchell M, Huang L, HERSH M, Stromberg A, Getchell T. Cellular and molecular characterization of oxidative stress in olfactory epithelium of Harlequin mutant mouse. J Neurosci Res. 2008;86:165-82 pubmed
Ferreira R, Neuparth M, Vitorino R, Appell H, Amado F, Duarte J. Evidences of apoptosis during the early phases of soleus muscle atrophy in hindlimb suspended mice. Physiol Res. 2008;57:601-11 pubmed
Kook S, Son Y, Chung S, Lee S, Kim J, Jeon Y, et al. Caspase-independent death of human osteosarcoma cells by flavonoids is driven by p53-mediated mitochondrial stress and nuclear translocation of AIF and endonuclease G. Apoptosis. 2007;12:1289-98 pubmed
Yoon Y, Yoon D, Lim I, Yoon S, Chung H, Rojo M, et al. Formation of elongated giant mitochondria in DFO-induced cellular senescence: involvement of enhanced fusion process through modulation of Fis1. J Cell Physiol. 2006;209:468-80 pubmed
Wang S, Yan Neale Y, Cai R, Alimov I, Cohen D. Activation of mitochondrial pathway is crucial for tumor selective induction of apoptosis by LAQ824. Cell Cycle. 2006;5:1662-8 pubmed
Parise R, Egorin M, Kanterewicz B, Taimi M, Petkovich M, Lew A, et al. CYP24, the enzyme that catabolizes the antiproliferative agent vitamin D, is increased in lung cancer. Int J Cancer. 2006;119:1819-28 pubmed
Strosznajder R, Gajkowska B. Effect of 3-aminobenzamide on Bcl-2, Bax and AIF localization in hippocampal neurons altered by ischemia-reperfusion injury. the immunocytochemical study. Acta Neurobiol Exp (Wars). 2006;66:15-22 pubmed
Ruchalski K, Mao H, Li Z, Wang Z, Gillers S, Wang Y, et al. Distinct hsp70 domains mediate apoptosis-inducing factor release and nuclear accumulation. J Biol Chem. 2006;281:7873-80 pubmed
Wu Y, Muldoon L, Neuwelt E. The chemoprotective agent N-acetylcysteine blocks cisplatin-induced apoptosis through caspase signaling pathway. J Pharmacol Exp Ther. 2005;312:424-31 pubmed
product information
Catalog Number :
sc-13116
Product Name :
AIF (E-1) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
E-1
Isotype :
IgG2b
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
AIF
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, IHC(P), FCM, ELISA
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA