product summary
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company name :
Santa Cruz Biotechnology
product type :
antibody
product name :
myogenin (F5D) Antibody
catalog :
sc-12732
quantity :
200 µg/ml
price :
316 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
F5D
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 84
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:250; loading ...
Pal A, Leung J, Ang G, Rao V, Pignata L, Lim H, et al. EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma. elife. 2020;9: pubmed publisher
  • immunocytochemistry; mouse; 1:200; loading ...; fig 9c
Srikuea R, Hirunsai M, Charoenphandhu N. Regulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing. Sci Rep. 2020;10:8239 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 4d
Fujimaki S, Seko D, Kitajima Y, Yoshioka K, Tsuchiya Y, Masuda S, et al. Notch1 and Notch2 Coordinately Regulate Stem Cell Function in the Quiescent and Activated States of Muscle Satellite Cells. Stem Cells. 2018;36:278-285 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 2b
Wang X, Zeng R, Xu H, Xu Z, Zuo B. The nuclear protein-coding gene ANKRD23 negatively regulates myoblast differentiation. Gene. 2017;629:68-75 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig s7a
  • western blot; mouse; loading ...; fig 1f, 2n, 3b, 4f
Kokabu S, Nakatomi C, Matsubara T, Ono Y, Addison W, Lowery J, et al. The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J Biol Chem. 2017;292:12885-12894 pubmed publisher
  • western blot; mouse; 1:200; fig 4d
Guo Y, Wang J, Zhu M, Zeng R, Xu Z, Li G, et al. Identification of MyoD-Responsive Transcripts Reveals a Novel Long Non-coding RNA (lncRNA-AK143003) that Negatively Regulates Myoblast Differentiation. Sci Rep. 2017;7:2828 pubmed publisher
  • western blot; mouse; 1:500; loading ...; fig 1
Mandai S, Furukawa S, Kodaka M, Hata Y, Mori T, Nomura N, et al. Loop diuretics affect skeletal myoblast differentiation and exercise-induced muscle hypertrophy. Sci Rep. 2017;7:46369 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 6b
Wang C, Wang M, Arrington J, Shan T, Yue F, Nie Y, et al. Ascl2 inhibits myogenesis by antagonizing the transcriptional activity of myogenic regulatory factors. Development. 2017;144:235-247 pubmed publisher
  • immunohistochemistry; human; 1:50
Yu L, Li J, Xu S, Navia Miranda M, Wang G, Duan Y. An Xp11.2 translocation renal cell carcinoma with SMARCB1 (INI1) inactivation in adult end-stage renal disease: a case report. Diagn Pathol. 2016;11:98 pubmed
  • immunohistochemistry; mouse; 1:200; fig 6
Southard S, Kim J, Low S, Tsika R, Lepper C. Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency. elife. 2016;5: pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 2b
Zhong Y, Zou L, Wang Z, Pan Y, Dai Z, Liu X, et al. Lrrc75b is a novel negative regulator of C2C12 myogenic differentiation. Int J Mol Med. 2016;38:1411-1418 pubmed publisher
  • western blot; mouse; loading ...; fig 1c
Ramazzotti G, Billi A, Manzoli L, Mazzetti C, Ruggeri A, Erneux C, et al. IPMK and β-catenin mediate PLC-β1-dependent signaling in myogenic differentiation. Oncotarget. 2016;7:84118-84127 pubmed publisher
  • western blot; human; 1:1000; fig 1
Moretti I, Ciciliot S, Dyar K, Abraham R, Murgia M, Agatea L, et al. MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. Nat Commun. 2016;7:12397 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 8c
  • EMSA; mouse; fig 7a
Li A, Zhang Y, Zhao Z, Wang M, Zan L. Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene. PLoS ONE. 2016;11:e0157445 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 3c
Murphy S, Kiely M, Jakeman P, Kiely P, Carson B. Optimization of an in vitro bioassay to monitor growth and formation of myotubes in real time. Biosci Rep. 2016;36: pubmed publisher
  • western blot; human; fig 2
Hansen N, Hjort L, Broholm C, Gillberg L, Schrölkamp M, Schultz H, et al. Metabolic and Transcriptional Changes in Cultured Muscle Stem Cells from Low Birth Weight Subjects. J Clin Endocrinol Metab. 2016;101:2254-64 pubmed publisher
  • chromatin immunoprecipitation; mouse
  • western blot; mouse; 1:1000; fig 3
Park S, Yun Y, Lim J, Kim M, Kim S, Kim J, et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun. 2016;7:10871 pubmed publisher
  • western blot; mouse; fig 1
Panda A, Abdelmohsen K, Martindale J, Di Germanio C, Yang X, Grammatikakis I, et al. Novel RNA-binding activity of MYF5 enhances Ccnd1/Cyclin D1 mRNA translation during myogenesis. Nucleic Acids Res. 2016;44:2393-408 pubmed publisher
  • western blot; human; 1:1000; fig 1
Martone J, Briganti F, Legnini I, Morlando M, Picillo E, Sthandier O, et al. The lack of the Celf2a splicing factor converts a Duchenne genotype into a Becker phenotype. Nat Commun. 2016;7:10488 pubmed publisher
  • immunocytochemistry; human; 1:400; fig 1
  • western blot; human; 1:3000; fig 2
  • chromatin immunoprecipitation; mouse; fig 6
  • immunocytochemistry; mouse; 1:400; fig 1
  • western blot; mouse; 1:3000; fig 6
Lee S, Won J, Yang J, Lee J, Kim S, Lee E, et al. AKAP6 inhibition impairs myoblast differentiation and muscle regeneration: Positive loop between AKAP6 and myogenin. Sci Rep. 2015;5:16523 pubmed publisher
  • western blot; mouse; 1:200; loading ...; fig 2b
Zhang Y, Li W, Zhu M, Li Y, Xu Z, Zuo B. FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB. Cell Signal. 2016;28:60-73 pubmed publisher
  • western blot; mouse; 1:200; fig 4c
Anderson K, Russell A, Foletta V. NDRG2 promotes myoblast proliferation and caspase 3/7 activities during differentiation, and attenuates hydrogen peroxide - But not palmitate-induced toxicity. FEBS Open Bio. 2015;5:668-81 pubmed publisher
  • western blot; mouse; fig 2
Yoo M, Kim B, Lee S, Jeong H, Park J, Seo D, et al. Syntaxin 4 regulates the surface localization of a promyogenic receptor Cdo thereby promoting myogenic differentiation. Skelet Muscle. 2015;5:28 pubmed publisher
  • western blot; mouse; 1:500
Feeney S, McGrath M, Sriratana A, Gehrig S, Lynch G, D Arcy C, et al. FHL1 reduces dystrophy in transgenic mice overexpressing FSHD muscular dystrophy region gene 1 (FRG1). PLoS ONE. 2015;10:e0117665 pubmed publisher
  • western blot; rat; 1:200; fig 7
Eilers W, Jaspers R, de Haan A, Ferrié C, Valdivieso P, Flück M. CaMKII content affects contractile, but not mitochondrial, characteristics in regenerating skeletal muscle. BMC Physiol. 2014;14:7 pubmed publisher
  • western blot; human
Ballarino M, Cazzella V, D Andrea D, Grassi L, Bisceglie L, Cipriano A, et al. Novel long noncoding RNAs (lncRNAs) in myogenesis: a miR-31 overlapping lncRNA transcript controls myoblast differentiation. Mol Cell Biol. 2015;35:728-36 pubmed publisher
  • western blot; human; 1:250; fig 3
Kabadi A, Thakore P, Vockley C, Ousterout D, Gibson T, Guilak F, et al. Enhanced MyoD-induced transdifferentiation to a myogenic lineage by fusion to a potent transactivation domain. ACS Synth Biol. 2015;4:689-99 pubmed publisher
  • western blot; human; fig s7
Huertas Martínez J, Rello Varona S, Herrero Martín D, Barrau I, García Monclús S, Sáinz Jaspeado M, et al. Caveolin-1 is down-regulated in alveolar rhabdomyosarcomas and negatively regulates tumor growth. Oncotarget. 2014;5:9744-55 pubmed
  • immunocytochemistry; mouse
Tanaka M, Kishimoto K, Okuno H, Saito H, Itoi E. Vitamin D receptor gene silencing effects on differentiation of myogenic cell lines. Muscle Nerve. 2014;49:700-8 pubmed publisher
Zhang S, Yang F, Huang Y, He L, Li Y, Wan Y, et al. ATF3 induction prevents precocious activation of skeletal muscle stem cell by regulating H2B expression. Nat Commun. 2023;14:4978 pubmed publisher
Tedesco B, Vendredy L, Adriaenssens E, Cozzi M, Asselbergh B, Crippa V, et al. HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathies. Autophagy. 2023;19:2217-2239 pubmed publisher
Singh A, Rai A, Weber A, Posern G. miRNA mediated downregulation of cyclase-associated protein 1 (CAP1) is required for myoblast fusion. Front Cell Dev Biol. 2022;10:899917 pubmed publisher
Chiu H, Loh A, Taneja R. Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma. Cell Death Dis. 2022;13:419 pubmed publisher
Becker R, Vergarajauregui S, Billing F, Sharkova M, Lippolis E, Mamchaoui K, et al. Myogenin controls via AKAP6 non-centrosomal microtubule-organizing center formation at the nuclear envelope. elife. 2021;10: pubmed publisher
Lv W, Jin J, Xu Z, Luo H, Guo Y, Wang X, et al. lncMGPF is a novel positive regulator of muscle growth and regeneration. J Cachexia Sarcopenia Muscle. 2020;11:1723-1746 pubmed publisher
Addison W, Hall K, Kokabu S, Matsubara T, Fu M, Gori F, et al. Zfp423 Regulates Skeletal Muscle Regeneration and Proliferation. Mol Cell Biol. 2019;39: pubmed publisher
Li J, Aponte Paris S, Thakur H, Kapiloff M, Dodge Kafka K. Muscle A-kinase-anchoring protein-β-bound calcineurin toggles active and repressive transcriptional complexes of myocyte enhancer factor 2D. J Biol Chem. 2019;294:2543-2554 pubmed publisher
Cichewicz M, Kiran M, Przanowska R, Sobierajska E, Shibata Y, Dutta A. MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD. Mol Cell Biol. 2018;38: pubmed publisher
Manandhar D, Song L, Kabadi A, Kwon J, Edsall L, Ehrlich M, et al. Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies. Nucleic Acids Res. 2017;45:11684-11699 pubmed publisher
Thomas J, Sznajder Ł, Bardhi O, Aslam F, Anastasiadis Z, Scotti M, et al. Disrupted prenatal RNA processing and myogenesis in congenital myotonic dystrophy. Genes Dev. 2017;31:1122-1133 pubmed publisher
Pryce B, Al Zahrani K, Dufresne S, Belkina N, Labrèche C, Patino Lopez G, et al. Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness. Skelet Muscle. 2017;7:3 pubmed publisher
Faralli H, Wang C, Nakka K, Benyoucef A, Sebastian S, Zhuang L, et al. UTX demethylase activity is required for satellite cell-mediated muscle regeneration. J Clin Invest. 2016;126:1555-65 pubmed publisher
Morena D, Maestro N, Bersani F, Forni P, Lingua M, Foglizzo V, et al. Hepatocyte Growth Factor-mediated satellite cells niche perturbation promotes development of distinct sarcoma subtypes. elife. 2016;5: pubmed publisher
Marchildon F, Lamarche É, Lala Tabbert N, St Louis C, Wiper Bergeron N. Expression of CCAAT/Enhancer Binding Protein Beta in Muscle Satellite Cells Inhibits Myogenesis in Cancer Cachexia. PLoS ONE. 2015;10:e0145583 pubmed publisher
Kaspar P, Zikova M, Bartunek P, Sterba J, Strnad H, Kren L, et al. The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin. Sci Rep. 2015;5:15090 pubmed publisher
Chen J, Wang Y, Hamed M, Lacroix N, Li Q. Molecular Basis for the Regulation of Transcriptional Coactivator p300 in Myogenic Differentiation. Sci Rep. 2015;5:13727 pubmed publisher
Fujita N, Ono M, Tomioka T, Deie M. Effects of hyperbaric oxygen at 1.25 atmospheres absolute with normal air on macrophage number and infiltration during rat skeletal muscle regeneration. PLoS ONE. 2014;9:e115685 pubmed publisher
Zhang D, Wang X, Li Y, Zhao L, Lu M, Yao X, et al. Thyroid hormone regulates muscle fiber type conversion via miR-133a1. J Cell Biol. 2014;207:753-66 pubmed publisher
Jiang C, Wen Y, Kuroda K, Hannon K, Rudnicki M, Kuang S. Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy. Dis Model Mech. 2014;7:997-1004 pubmed publisher
Storbeck C, Al Zahrani K, Sriram R, Kawesa S, O Reilly P, Daniel K, et al. Distinct roles for Ste20-like kinase SLK in muscle function and regeneration. Skelet Muscle. 2013;3:16 pubmed publisher
Romer A, Singh J, Rattan S, Krauss R. Smooth muscle fascicular reorientation is required for esophageal morphogenesis and dependent on Cdo. J Cell Biol. 2013;201:309-23 pubmed publisher
Bichsel C, Neeld D, Hamazaki T, Chang L, Yang L, Terada N, et al. Direct reprogramming of fibroblasts to myocytes via bacterial injection of MyoD protein. Cell Reprogram. 2013;15:117-25 pubmed publisher
Ambrosio R, Damiano V, Sibilio A, De Stefano M, Avvedimento V, Salvatore D, et al. Epigenetic control of type 2 and 3 deiodinases in myogenesis: role of Lysine-specific Demethylase enzyme and FoxO3. Nucleic Acids Res. 2013;41:3551-62 pubmed publisher
Suzuki T, Do M, Sato Y, Ojima K, Hara M, Mizunoya W, et al. Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression. Int J Biochem Cell Biol. 2013;45:476-82 pubmed publisher
Marzi M, Puggioni E, Dall olio V, Bucci G, Bernard L, Bianchi F, et al. Differentiation-associated microRNAs antagonize the Rb-E2F pathway to restrict proliferation. J Cell Biol. 2012;199:77-95 pubmed publisher
Tsai C, Huang T, Ma H, Chiang E, Hung S. Isolation of mesenchymal stem cells from shoulder rotator cuff: a potential source for muscle and tendon repair. Cell Transplant. 2013;22:413-22 pubmed publisher
Cazzella V, Martone J, Pinnarò C, Santini T, Twayana S, Sthandier O, et al. Exon 45 skipping through U1-snRNA antisense molecules recovers the Dys-nNOS pathway and muscle differentiation in human DMD myoblasts. Mol Ther. 2012;20:2134-42 pubmed publisher
Tran T, Shi X, Zaia J, Ai X. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem. 2012;287:32651-64 pubmed
Gruenbaum Cohen Y, Harel I, Umansky K, Tzahor E, Snapper S, Shilo B, et al. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A. 2012;109:11211-6 pubmed publisher
Faenza I, Blalock W, Bavelloni A, Schoser B, Fiume R, Pacella S, et al. A role for PLC?1 in myotonic dystrophies type 1 and 2. FASEB J. 2012;26:3042-8 pubmed publisher
Stains C, Tedford N, Walkup T, Luković E, Goguen B, Griffith L, et al. Interrogating signaling nodes involved in cellular transformations using kinase activity probes. Chem Biol. 2012;19:210-7 pubmed publisher
Pronsato L, Boland R, Milanesi L. Testosterone exerts antiapoptotic effects against H2O2 in C2C12 skeletal muscle cells through the apoptotic intrinsic pathway. J Endocrinol. 2012;212:371-81 pubmed publisher
Lahariya C, Mishra A, Nandan D, Gautam P, Gupta S. Additional cash incentive within a conditional cash transfer scheme: a 'controlled before and during' design evaluation study from India. Indian J Public Health. 2011;55:115-20 pubmed publisher
Kim J, Jang S, Kim C, An J, Kang E, Choi K. Tip60 regulates myoblast differentiation by enhancing the transcriptional activity of MyoD via their physical interactions. FEBS J. 2011;278:4394-404 pubmed publisher
Xiao F, Wang H, Fu X, Li Y, Ma K, Sun L, et al. Oncostatin M inhibits myoblast differentiation and regulates muscle regeneration. Cell Res. 2011;21:350-64 pubmed publisher
Miyake M, Hayashi S, Iwasaki S, Uchida T, Watanabe K, Ohwada S, et al. TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells. J Cell Physiol. 2011;226:1128-36 pubmed publisher
Henningsen J, Rigbolt K, Blagoev B, Pedersen B, Kratchmarova I. Dynamics of the skeletal muscle secretome during myoblast differentiation. Mol Cell Proteomics. 2010;9:2482-96 pubmed publisher
Gao C, Liu Y, Lam M, Kao H. Histone deacetylase 7 (HDAC7) regulates myocyte migration and differentiation. Biochim Biophys Acta. 2010;1803:1186-97 pubmed publisher
Kitabayashi K, Siltanen A, Pätilä T, Mahar M, Tikkanen I, Koponen J, et al. Bcl-2 expression enhances myoblast sheet transplantation therapy for acute myocardial infarction. Cell Transplant. 2010;19:573-88 pubmed publisher
Di Felice V, Ardizzone N, De Luca A, Marciano V, Marino Gammazza A, Macaluso F, et al. OPLA scaffold, collagen I, and horse serum induce an higher degree of myogenic differentiation of adult rat cardiac stem cells. J Cell Physiol. 2009;221:729-39 pubmed publisher
Crassous B, Richard Bulteau H, Deldicque L, Serrurier B, Pasdeloup M, Francaux M, et al. Lack of effects of creatine on the regeneration of soleus muscle after injury in rats. Med Sci Sports Exerc. 2009;41:1761-9 pubmed publisher
Foletta V, Prior M, Stupka N, Carey K, Segal D, Jones S, et al. NDRG2, a novel regulator of myoblast proliferation, is regulated by anabolic and catabolic factors. J Physiol. 2009;587:1619-34 pubmed publisher
Wang K, Wang C, Xiao F, Wang H, Wu Z. JAK2/STAT2/STAT3 are required for myogenic differentiation. J Biol Chem. 2008;283:34029-36 pubmed publisher
Ramazzotti G, Faenza I, Gaboardi G, Piazzi M, Bavelloni A, Fiume R, et al. Catalytic activity of nuclear PLC-beta(1) is required for its signalling function during C2C12 differentiation. Cell Signal. 2008;20:2013-21 pubmed publisher
Kook S, Son Y, Lee K, Lee H, Chung W, Choi K, et al. Hypoxia affects positively the proliferation of bovine satellite cells and their myogenic differentiation through up-regulation of MyoD. Cell Biol Int. 2008;32:871-8 pubmed publisher
Håkelien A, Delbarre E, Gaustad K, Buendia B, Collas P. Expression of the myodystrophic R453W mutation of lamin A in C2C12 myoblasts causes promoter-specific and global epigenetic defects. Exp Cell Res. 2008;314:1869-80 pubmed publisher
Richard Bulteau H, Serrurier B, Crassous B, Banzet S, Peinnequin A, Bigard X, et al. Recovery of skeletal muscle mass after extensive injury: positive effects of increased contractile activity. Am J Physiol Cell Physiol. 2008;294:C467-76 pubmed
Kook S, Choi K, Son Y, Lee K, Hwang I, Lee H, et al. Involvement of p38 MAPK-mediated signaling in the calpeptin-mediated suppression of myogenic differentiation and fusion in C2C12 cells. Mol Cell Biochem. 2008;310:85-92 pubmed
Inoue M, Wu H. Immunohistochemical detection of myogenin and p21 in methylcholanthrene-induced mouse rhabdomyosarcomas. Int J Exp Pathol. 2006;87:445-50 pubmed
Faenza I, Ramazzotti G, Bavelloni A, Fiume R, Gaboardi G, Follo M, et al. Inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter. Endocrinology. 2007;148:1108-17 pubmed
Ko J, Kimura Y, Matsuura K, Yamamoto H, Gondo T, Inui M. PDZRN3 (LNX3, SEMCAP3) is required for the differentiation of C2C12 myoblasts into myotubes. J Cell Sci. 2006;119:5106-13 pubmed
Kim H, Lee Y, Sivaprasad U, Malhotra A, Dutta A. Muscle-specific microRNA miR-206 promotes muscle differentiation. J Cell Biol. 2006;174:677-87 pubmed
Jones N, Tyner K, Nibarger L, Stanley H, Cornelison D, Fedorov Y, et al. The p38alpha/beta MAPK functions as a molecular switch to activate the quiescent satellite cell. J Cell Biol. 2005;169:105-16 pubmed
Huh M, Parker M, Scime A, Parks R, Rudnicki M. Rb is required for progression through myogenic differentiation but not maintenance of terminal differentiation. J Cell Biol. 2004;166:865-76 pubmed
product information
Catalog Number :
sc-12732
Product Name :
myogenin (F5D) Antibody
Product Type :
Monoclonal
Host Species :
mouse
Clonality :
monoclonal
Clone Name :
F5D
Isotype :
IgG1
Size :
200 µg/ml
List Price :
316 USD
Immunogen :
myogenin
Specificity :
mouse, rat, human
Uses :
WB, IP, IF, IHC(P)
company information
Santa Cruz Biotechnology
2145 Delaware Avenue
Santa Cruz, CA 95060
scbt@scbt.com
https://www.scbt.com
1.800.457.3801
headquarters: USA