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company name :
Rockland Immunochemicals
product type :
other
product name :
HeLa Whole Cell Lysate MG-132 Treated
catalog :
W09-001-GV9
quantity :
500 µg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
W09-001-GV9
Name :
HeLa Whole Cell Lysate - MG-132 Stimulated - W09-001-GV9
Display Name :
HeLa Whole Cell Lysate MG-132 Treated
Application Note :
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
Buffer :
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Cell Line :
HeLa - Human epidermoid carcinoma
Concentration Value :
1mg/mL
Concentration Definition :
by modified Lowry assay
Conjugation :
(None)
Size :
500 µg
Default Unit :
µg
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is three (3) months from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Induction :
MG-132
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
HeLa cells were grown in Dulbecco’s medium supplemented with 10% fetal bovine serum. Cells were treated O/N with 0.2µg/mL of MG-132 in order to reduce degradation of ubiquitin-conjugated proteins by the proteasome. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.
Species of Origin :
Human
Stabilizer :
10% (v/v) Glycerol
Storage :
Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Synonyms :
HeLa Lysate MG132 stimulated, Cell Lysate, MG-132 Stimulated Lysate HeLa cell, HeLa lysate MG-132 treated
Background :
Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
Low Endotoxin :
No
Lysate Fractionation :
Whole Cell Lysate
Lysate Stimulation :
MG-132
Lysate Tissue Culture vs Primary Tissue :
Tissue Culture
Sample Size :
No
Application Text :
Western Blot,
Other :
User Optimized
Category :
Cell Lysates
Conjugation Name :
Unconjugated
Primary Image Name :
SDS-PAGE of HeLa Whole Cell Lysate MG-132 Stimulated
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.