HT-29 Lysate | Rockland Immunochemicals W09-001-BS0 product information

product summary

company name :

Rockland Immunochemicals

product type :

other

product name :

HT-29 Lysate

catalog :

W09-001-BS0

quantity :

500 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

W09-001-BS0

Name :

HT-29 Whole Cell Lysate - W09-001-BS0

Display Name :

HT-29 Lysate

Application Note :

Ready-to-use HT-29 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.

Buffer :

1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)

Cell Line :

Human - colorectal carcinoma

Clone ID :

HT-29

Concentration Value :

1.0 mg/ml

Concentration Definition :

by modified Lowry assay

Conjugation :

(None)

Size :

500 µg

Default Unit :

µg

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is three (3) months from date of opening.

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Human Disclaimer Note :

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Induction :

None (Control)

Other Performance Data :

User Optimized

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

HT-29 cells were grown in Dulbecco’s medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate, 1 µM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

Species of Origin :

Human

Stabilizer :

10% (v/v) Glycerol

Storage :

Store HT-29 Whole Cell Lysate at -70° C or COLDER. For extended storage, whole cell lysate to minimize freeze/thaw cycles.

Synonyms :

HT29 Lysate, Cell Lysate, HT-29 lysate

Background :

Ready-to-use HT-29 Whole Cell Lysates produced by Rockland Immunochemicals are derived from cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Low Endotoxin :

Lysate Fractionation :

Whole Cell Lysate

Lysate Stimulation :

Not Stimulated

Lysate Tissue Culture vs Primary Tissue :

Tissue Culture

Sample Size :

Application Text :

Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Cell Lysates

Conjugation Name :

Unconjugated

more info or order :

Rockland Immunochemicals product webpage